This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid...This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid methyl esters （FAMEs）, and a polymerase chain reaction （PCR） test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.展开更多
为提高蝴蝶兰开花品质,以蝴蝶兰品种"V31"(Dtps.Tailin Red Angel"V31")为材料,研究花芽分化后期不同肥水、光照、温度等因素对开花品质的影响,结果表明:N∶P2O5∶K2O为10∶30∶20的花多多专用肥、肥料浓度1 500倍...为提高蝴蝶兰开花品质,以蝴蝶兰品种"V31"(Dtps.Tailin Red Angel"V31")为材料,研究花芽分化后期不同肥水、光照、温度等因素对开花品质的影响,结果表明:N∶P2O5∶K2O为10∶30∶20的花多多专用肥、肥料浓度1 500倍、2肥1水的肥水管理模式、光照强度处于15 000～25 000Lx、日温/夜温为26～28℃/16～18℃等条件下蝴蝶兰花苞数最多,花穗最长;施用N∶P2O5∶K2O为20∶20∶20的花多多专用肥,蝴蝶兰花径最大;温度、光照强度对开花进度影响显著,高温、强光条件下,30d可比低温、弱光照条件下多开2～4朵花。展开更多
基金the National High Technology Research and Development Program of China (2006AA10A211)National Natural Science Foundation of China (30671397)Hangzhou Agricultural Development Foundation,China (2007-2008)
文摘This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch＇s postulate test, Biolog, fatty acid methyl esters （FAMEs）, and a polymerase chain reaction （PCR） test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.
