To investigate the levels of polymorphisms and Mendelian segregation ratio in clone “TB01” (P. tomentosa×P. bolleana) ×clone “LM50” (P. tomentosa) BC 1 population at the entire genome level, ampl...To investigate the levels of polymorphisms and Mendelian segregation ratio in clone “TB01” (P. tomentosa×P. bolleana) ×clone “LM50” (P. tomentosa) BC 1 population at the entire genome level, amplified fragment length polymorphisms (AFLPs) analysis was conducted for both parents and 120 progenies. Forty one pairs of selective primers were used to detect 2?707 bands, of which 712 (26.4%) were polymorphic. Chi\|square tests were performed to examine if the observed genotypic frequencies of AFLP loci deviated from expected 1∶1 Mendelian segregation ratio ( P <0.01) in BC 1 population. Among the 712 loci 571 (80.2%) fit to Mendelian 1∶1 segregation ratio, corresponding to DNA polymorphisms heterozygous in one parent and a null in the other. The result shows that the AFLP markers are very suitable for fingerprinting and genetic mapping in the Chinese white poplar (Populus tomentosa Carr.).展开更多
In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector...In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector strategy,we first inserted the remolded tPA gene into the Ti-derived plasmid, PGA643 and then transferred,in a triparental mating system,the plasmid into a strain of Agrobacterium tumefaciens,A281, that contained vir-DNA.Finally poplar cells were transformed with A. tumefaciens using leaf discus method. The transformed cells were selected in culture containing kanamycin and in siyu hybridization.Successful generation of transgenic poplar was demonstrated by molecular hybridization in the cultivated plants and detection of tPA gene product.展开更多
文摘To investigate the levels of polymorphisms and Mendelian segregation ratio in clone “TB01” (P. tomentosa×P. bolleana) ×clone “LM50” (P. tomentosa) BC 1 population at the entire genome level, amplified fragment length polymorphisms (AFLPs) analysis was conducted for both parents and 120 progenies. Forty one pairs of selective primers were used to detect 2?707 bands, of which 712 (26.4%) were polymorphic. Chi\|square tests were performed to examine if the observed genotypic frequencies of AFLP loci deviated from expected 1∶1 Mendelian segregation ratio ( P <0.01) in BC 1 population. Among the 712 loci 571 (80.2%) fit to Mendelian 1∶1 segregation ratio, corresponding to DNA polymorphisms heterozygous in one parent and a null in the other. The result shows that the AFLP markers are very suitable for fingerprinting and genetic mapping in the Chinese white poplar (Populus tomentosa Carr.).
文摘In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector strategy,we first inserted the remolded tPA gene into the Ti-derived plasmid, PGA643 and then transferred,in a triparental mating system,the plasmid into a strain of Agrobacterium tumefaciens,A281, that contained vir-DNA.Finally poplar cells were transformed with A. tumefaciens using leaf discus method. The transformed cells were selected in culture containing kanamycin and in siyu hybridization.Successful generation of transgenic poplar was demonstrated by molecular hybridization in the cultivated plants and detection of tPA gene product.
