目的:探讨纤维内镜评估吞咽功能(fiberoptic endoscopic evaluation of swallowing, FEES)在累及舌根的舌癌(both the oral tongue and the base of the tongue, OBOT)患者中的临床应用。方法:对2022年4月至2023年5月于我院初诊为累及O...目的:探讨纤维内镜评估吞咽功能(fiberoptic endoscopic evaluation of swallowing, FEES)在累及舌根的舌癌(both the oral tongue and the base of the tongue, OBOT)患者中的临床应用。方法:对2022年4月至2023年5月于我院初诊为累及OBOT病变的患者术前、术后1周、1月和1年进行吞咽功能评估。吞咽功能的评估包括MD安德森吞咽困难量表(MD Anderson Dysphagia Inventory, MDADI)、华盛顿大学生活质量问卷(University of Washington Quality-of-Life Questionnaire, UW-QOL)、头颈癌表现状态量表(Performance Status Scale for Head and Neck Cancer, PSS-HN)、洼田饮水试验(water swallow test, WST)、舌运动测量和FEES[包括渗透-吸入量表(Penetration-Aspiration Scale, PAS)和口咽吞咽效率(oropharyngeal swallowing efficiency, OPSE)]。并对FEES的评估OBOT的可靠性进行统计分析。结果:共有21例患者纳入研究。FEES具有较高的诊断价值(ROC曲线下面积=0.916)。FEES结果与WST和MDADI评分显著相关(P<0.001)。术后1年,累及OBOT病变患者的吞咽功能恢复到术前水平,但OPSE明显下降,生活质量仍受影响(P<0.001)。结论:FEES是评估累及OBOT病变患者术后吞咽功能的可靠方法,值得临床推广。在术后随访中,虽然这些患者的吞咽功能可以恢复,但应注意吞咽效率和生活质量。展开更多
Background:The role of Claudin-1 in tongue squamous cell carcinoma(TSCC)metastasis needs further clarification,particularly its impact on cell migration.Herein,our study aims to investigate the role of Claudin-1 in TS...Background:The role of Claudin-1 in tongue squamous cell carcinoma(TSCC)metastasis needs further clarification,particularly its impact on cell migration.Herein,our study aims to investigate the role of Claudin-1 in TSCC cell migration and its underlying mechanisms.Methods:36 TSCC tissue samples underwent immunohistochemical staining for Claudin-1.Western blotting and immunofluorescence analyses were conducted to evaluate Claudin-1 expression and distribution in TSCC cells.Claudin-1 knockdown cell lines were established using short hairpin RNA transfection.Migration effects were assessed through wound healing assays.Furthermore,the expression of EMTassociated molecules was measured via western blotting.Results:Claudin-1 expression decreased as TSCC malignancy increased.Adenosine monophosphate–activated protein kinase(AMPK)activation led to increased Claudin-1 expression and membrane translocation,inhibiting TSCC cell migration and epithelial–mesenchymal transition(EMT).Conversely,Claudin-1 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.Conclusions:Our results indicated that AMPK activation suppresses TSCC cell migration by targeting Claudin-1 and EMT pathways.展开更多
文摘目的:探讨纤维内镜评估吞咽功能(fiberoptic endoscopic evaluation of swallowing, FEES)在累及舌根的舌癌(both the oral tongue and the base of the tongue, OBOT)患者中的临床应用。方法:对2022年4月至2023年5月于我院初诊为累及OBOT病变的患者术前、术后1周、1月和1年进行吞咽功能评估。吞咽功能的评估包括MD安德森吞咽困难量表(MD Anderson Dysphagia Inventory, MDADI)、华盛顿大学生活质量问卷(University of Washington Quality-of-Life Questionnaire, UW-QOL)、头颈癌表现状态量表(Performance Status Scale for Head and Neck Cancer, PSS-HN)、洼田饮水试验(water swallow test, WST)、舌运动测量和FEES[包括渗透-吸入量表(Penetration-Aspiration Scale, PAS)和口咽吞咽效率(oropharyngeal swallowing efficiency, OPSE)]。并对FEES的评估OBOT的可靠性进行统计分析。结果:共有21例患者纳入研究。FEES具有较高的诊断价值(ROC曲线下面积=0.916)。FEES结果与WST和MDADI评分显著相关(P<0.001)。术后1年,累及OBOT病变患者的吞咽功能恢复到术前水平,但OPSE明显下降,生活质量仍受影响(P<0.001)。结论:FEES是评估累及OBOT病变患者术后吞咽功能的可靠方法,值得临床推广。在术后随访中,虽然这些患者的吞咽功能可以恢复,但应注意吞咽效率和生活质量。
基金supported by grants from National Natural Science Foundation of China(no.:82174020 and no.:31301137)Shanxi Basic Research Program of China(202103021224378)Shanxi Bethune Hospital Talent Introduction Research Start-up Fund of China(2022RC13)。
文摘Background:The role of Claudin-1 in tongue squamous cell carcinoma(TSCC)metastasis needs further clarification,particularly its impact on cell migration.Herein,our study aims to investigate the role of Claudin-1 in TSCC cell migration and its underlying mechanisms.Methods:36 TSCC tissue samples underwent immunohistochemical staining for Claudin-1.Western blotting and immunofluorescence analyses were conducted to evaluate Claudin-1 expression and distribution in TSCC cells.Claudin-1 knockdown cell lines were established using short hairpin RNA transfection.Migration effects were assessed through wound healing assays.Furthermore,the expression of EMTassociated molecules was measured via western blotting.Results:Claudin-1 expression decreased as TSCC malignancy increased.Adenosine monophosphate–activated protein kinase(AMPK)activation led to increased Claudin-1 expression and membrane translocation,inhibiting TSCC cell migration and epithelial–mesenchymal transition(EMT).Conversely,Claudin-1 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.Conclusions:Our results indicated that AMPK activation suppresses TSCC cell migration by targeting Claudin-1 and EMT pathways.
