Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited ...Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited number of countries,primarily as a result of imported cases(El Eid et al.,2022;Tan and Gao,2022).展开更多
Since the outbreak of the coronavirus disease 2019(COVID-19)epidemic in 2019,the public health system has faced enormous challenges.Tracking the individuals who test positive for severe acute respiratory syndrome coro...Since the outbreak of the coronavirus disease 2019(COVID-19)epidemic in 2019,the public health system has faced enormous challenges.Tracking the individuals who test positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a key step for interrupting chains of transmission of SARS-CoV-2 and reducing COVID-19-associated mortality.With the increasing of asymptomatic infections,it is difficult to track asymptomatic infections through epidemiological surveys and virus whole-genome sequencing.However,due to the cross-reactivity of neutralizing antibodies produced by multiple virus subtypes,neutralizing antibody detection cannot be used to determine whether an individual has a history of infection with a specific subtype of SARS-CoV-2.We recruited 4 human leukocyte antigen A2(HLA-A2)infections,15 individuals who received three doses of inactivated vaccines,and 30 breakthrough infections after vaccination and discussed a case-tracking approach to detect epitope-specific CD8^(+)T cells in the peripheral blood of close contacts,including accurate HLA typing based on ribonucleic acid(RNA)-sequencing and flow cytometry data and the comparison and characterization of SARS-CoV-2 HLA-A2 and HLA-A24 epitope-specific CD8^(+)T cells.From individuals who received three doses of inactivated vaccine,we observed that the CD8^(+)T cell specificity for ancestral epitopes was significantly higher than for mutated epitopes,and the fold change of CD8^(+)T cells corresponding to mutated epitopes relative to ancestral epitopes was less than 1.The enzyme-linked immunospot(ELISpot)results further validate this result.This study forms a“method for understanding the infection history of SARS-CoV-2 subtypes based on the proportion of epitope-specific CD8^(+)T cells in the peripheral blood of subjects”,covering up to 46%of the population,including HLA-A2+and HLA-A24+donors,providing a novel method for SARS-CoV-2 infected case tracing.展开更多
This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen,China,encompassing clinical symptomatology,therapeutic approaches,epidemiological research,and comprehensive laboratory tests,aiming to...This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen,China,encompassing clinical symptomatology,therapeutic approaches,epidemiological research,and comprehensive laboratory tests,aiming to establish a robust reference for future monkeypox mitigation and management strategies.The investigation involved a thorough investigation of all identified positive cases,including extensive molecular analysis by nucleic acid detection and whole-genome sequencing of the monkeypox virus.Contact tracing and containment of the infected individuals were also undertaken.Three distinct monkeypox cases were identified in this unique outbreak,exhibiting mild and atypical clinical manifestations,primarily fever and rash.All cases were associated with a single transmission chain,primarily facilitated through close contact and homosexual behavior,indicative of a high-risk factor for monkeypox transmission.展开更多
基金supported in part by the National Key Research and Development Program of China (2022YFC2303401, 2021YFA1201003, 2021YFC2301605, 2022YFC2304100, 2022YFC2304101, 2022YFC0869900)the National Natural Science Foundation of China (82241066)。
文摘Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited number of countries,primarily as a result of imported cases(El Eid et al.,2022;Tan and Gao,2022).
基金the Key Project of Shenzhen Science and Technology Innovation Commission(JCYJ20210324115411030)Natural Science Foundation of China(92169102)+10 种基金R&D Program of Guangzhou Laboratory(SRPG22-006)Sanming Project of Medicine in Shenzhen(SZSM202211023)GuangDong Basic and Applied Basic Research Foundation(2022B1515120043)the Open Project Fund of Guangdong Provincial People’s Hospital(YKY-KF202208)National Natural Science Foundation of China(81902097)Funding by Science and Technology Projects in Guangzhou(SL2023A04J01160)the Guangdong Basic andApplied Basic Research Foundation(2023A1515140117)the fellowship of China Postdoctoral Science Foundation(2023TQ0136,2023M741379)supported by grants from the National Key Research and Development Plan(2023YFE0118700,2021YFC2301604)the Fundamental Research Funds for the Central Q4 Universities(21623406).
文摘Since the outbreak of the coronavirus disease 2019(COVID-19)epidemic in 2019,the public health system has faced enormous challenges.Tracking the individuals who test positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a key step for interrupting chains of transmission of SARS-CoV-2 and reducing COVID-19-associated mortality.With the increasing of asymptomatic infections,it is difficult to track asymptomatic infections through epidemiological surveys and virus whole-genome sequencing.However,due to the cross-reactivity of neutralizing antibodies produced by multiple virus subtypes,neutralizing antibody detection cannot be used to determine whether an individual has a history of infection with a specific subtype of SARS-CoV-2.We recruited 4 human leukocyte antigen A2(HLA-A2)infections,15 individuals who received three doses of inactivated vaccines,and 30 breakthrough infections after vaccination and discussed a case-tracking approach to detect epitope-specific CD8^(+)T cells in the peripheral blood of close contacts,including accurate HLA typing based on ribonucleic acid(RNA)-sequencing and flow cytometry data and the comparison and characterization of SARS-CoV-2 HLA-A2 and HLA-A24 epitope-specific CD8^(+)T cells.From individuals who received three doses of inactivated vaccine,we observed that the CD8^(+)T cell specificity for ancestral epitopes was significantly higher than for mutated epitopes,and the fold change of CD8^(+)T cells corresponding to mutated epitopes relative to ancestral epitopes was less than 1.The enzyme-linked immunospot(ELISpot)results further validate this result.This study forms a“method for understanding the infection history of SARS-CoV-2 subtypes based on the proportion of epitope-specific CD8^(+)T cells in the peripheral blood of subjects”,covering up to 46%of the population,including HLA-A2+and HLA-A24+donors,providing a novel method for SARS-CoV-2 infected case tracing.
基金the Science and Technology Planning Project of Guangdong Province of China(Grant 2021 B1212030009)Sanming Project of Medicine in Shenzhen(No.SZSM202011008)+1 种基金Research Foundation of Shenzhen Science and Technology Emergency Key Technology Program(JSGG20220301090007009)Shenzhen Key Medical Discipline Construction Fund(SZXK064).
文摘This paper comprehensively analyses the first-ever monkeypox outbreak in Shenzhen,China,encompassing clinical symptomatology,therapeutic approaches,epidemiological research,and comprehensive laboratory tests,aiming to establish a robust reference for future monkeypox mitigation and management strategies.The investigation involved a thorough investigation of all identified positive cases,including extensive molecular analysis by nucleic acid detection and whole-genome sequencing of the monkeypox virus.Contact tracing and containment of the infected individuals were also undertaken.Three distinct monkeypox cases were identified in this unique outbreak,exhibiting mild and atypical clinical manifestations,primarily fever and rash.All cases were associated with a single transmission chain,primarily facilitated through close contact and homosexual behavior,indicative of a high-risk factor for monkeypox transmission.
