The Chinese herbs Herba Epimedii, Fructus Ligustri Lucidi and Rhizoma Polygonati were injected into Parkinson＇s disease mice established via intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridin...The Chinese herbs Herba Epimedii, Fructus Ligustri Lucidi and Rhizoma Polygonati were injected into Parkinson＇s disease mice established via intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine hydrochloride. The selective monoamine oxidase B inhibitor selegiline was used as a positive control drug. After successive administration for 4 weeks, Herba Epimedii could downregulate the expression of caspase-3 and increase the brain-derived neurotrophic factor level, as well as increase tyrosine hydroxylase activity in the substantia nigra of Parkinson＇s disease mouse models. Rhizoma Polygonaticould downregulate the expression of caspase-3 and FasL, and increase neural growth factor and brain-derived neurotrophic factor levels. Fructus Ligustn Lucidi could downregulate caspase-3 expression. Rhizoma Polygonati and Fructus Ligustn Lucidi did not produce obvious effects on tyrosine hydroxylase activity. Herba Epimedii and Fructus Ligustri Lucidi yielded similar effects on apoptosis-promoting factors to those elicited by selegiline. Herba Epimedii and Rhizoma Polygonati significantly increased the levels of neurotrophic factors compared with selegiline. Herba Epimedii significantly increased tyrosine hydroxylase activity compared with selegiline. It is indicated that the kidney-tonifying Chinese herbal preparation can downregulate the expression of apoptosis-promoting factors, increase neurotrophic factors levels in the substantia nigra and striatum, as well as increase tyrosine hydroxylase activity in the substantia nigra of Parkinson＇s disease mouse models, thereby exerting a stronger or similar neuroprotective effects compared with selegiline.展开更多
The present study involved a questionnaire survey of 156 mothers that gave birth to children with neural tube defects or had a history of pregnancy resulting in children with neural tube defects （case group） and 156...The present study involved a questionnaire survey of 156 mothers that gave birth to children with neural tube defects or had a history of pregnancy resulting in children with neural tube defects （case group） and 156 control mothers with concurrent healthy children （control group） as well as detection of mitochondrial membrane transporter protein gene [uncoupling protein 2 （UCP2）] polymorphism. The maternal UCP2 3＇ untranslated region （UTR） D/D genotype and D allele frequency were significantly higher in the case group compared with the control group （odds ratio （OR） 3.233; 95% confidence interval （C/） 1.103 9.476; P= 0.040; OR： 3.484; 95% CI： for neural tube defects 2.109 5.753; P 〈 0.001）. Univariate and multivariate logistic regression analysis of risk factors for neural tube defects showed that a matemal UCP2 3＇ UTR D/D genotype was negatively interacted with the mothers＇ consumption of frequent fresh fruit and vegetables （S = 0.007）, positively interacted with the mothers＇ frequency of germinated potato consumption （S = 2.15） and positively interacted with the mothers＇ body mass index （S = 3.50）. These findings suggest that maternal UCP2 3＇ UTR gene polymorphism, pregnancy time, consumption of germinated potatoes and body mass index are associated with an increased risk for neural tube defects in children from mothers living in Shanxi province, China. Moreover, there is an apparent gene-environment interaction involved in the development of neural tube defects in offspring.展开更多
In the process of engineering construction such as tunnels and slopes,rock mass is frequently subjected to multiple levels of loading and unloading,while previous research ignores the impact of unloading rate on the s...In the process of engineering construction such as tunnels and slopes,rock mass is frequently subjected to multiple levels of loading and unloading,while previous research ignores the impact of unloading rate on the stability of rock mass.A number of uniaxial multi-level cyclic loading-unloading experiments were conducted to better understand the effect of unloading rate on the deformation behavior,energy evolution,and damage properties of rock-like material.The experimental results demonstrated that the unloading rate and relative cyclic number clearly influence the deformation behavior and energy evo-lution of rock-like samples.In particular,as the relative cyclic number rises,the total strain and reversible strain both increase linearly,while the total energy density,elastic energy density,and dissipated energy density all rise nonlinearly.In contrast,the irreversible strain first decreases quickly,then stabilizes,and finally rises slowly.As the unloading rate increases,the total strain and reversible strain both increase,while the irreversible strain decreases.The dissipated energy damage was examined in light of the aforementioned experimental findings.The accuracy of the proposed damage model,which takes into account the impact of the unloading rate and relative cyclic number,is then confirmed by examining the consistency between the model predicted and the experimental results.The proposed damage model will make it easier to foresee how the multi-level loading-unloading cycles will affect the rock-like materials.展开更多
OBJECTIVE： To investigate the distribution characteristics of TCM syndromes and the related herbal prescriptions for malignant tumors （MT）. METHODS： A clinical database of the TCM syndromes and the herbal prescrip...OBJECTIVE： To investigate the distribution characteristics of TCM syndromes and the related herbal prescriptions for malignant tumors （MT）. METHODS： A clinical database of the TCM syndromes and the herbal prescriptions in treatment of 136 MT patients were established. The data were then analyzed using cluster and frequency analysis. RESULTS： According to the cluster analysis, the TCM syndromes in MT patients mainly included two patterns： deficiency of both Qi and Yin and internal accumulation of toxic heat. The commonlyprescribed herbs were Huangqi （Astraglus）, Nuizhenzi （Fructus Ligustri Lucidi）, Lingzhi （Ganoderma Lucidum）, Huaishan （Dioscorea Opposita）, Xiakucao （Prunella Vulgaris）, and Baihuasheshecao （Herba Hedyotidis）. CONCLUSION： Deficiency of Qi and Yin is the pri- mary syndrome of MT, and internal accumulation of toxic heat is the secondary syndrome. The herbs for Qi mainly heat-cl supplementation and Yin houris used, with earance and the assistance of detoxification. hment are herbs for展开更多
OBJECTIVE： To test the in vitro antiviral activity of a crude tissue extract （CTE/from the earthworm Eisenia fetida, determine any effective components in the CTE, andelucidate possiblemechanismsofaction. METHODS： ...OBJECTIVE： To test the in vitro antiviral activity of a crude tissue extract （CTE/from the earthworm Eisenia fetida, determine any effective components in the CTE, andelucidate possiblemechanismsofaction. METHODS： A CTE was made by homogenizing earthworms, followed by treatment with ammoni- um sulfate, then thermal denaturation. Inhibition of virus-induced cytopathic effect （CPE） was used to assess antiviral activity. Chromatographic analy- sis was used to identify effective components in the CTE. RESULTS： The CTE inhibited viral CPE at non-cyto- toxic concentrations. Chromatography indicated that antiviral components corresponded to three active peaks indicative of proteases, nucleases and lysozymes. For adenoviruses, reduction in viral ac- tivity occurred for 100 lag/mL CTE. The reduction in adenoviral activity for four fractions was 100%, 91.8%, 86.9%, and 94.7%. For influenza viruses, re- duction in viral activity of 100%, 86.6%, 69.1% and 88.3% was observed for 37 pg/mL CTE. In addition, three active fractions mixture had stronger antiviral activity （98.7% and 96.7%） than three fractions alone.Gel electrophoresis results indicated that nu- cleases from E. fetida could degrade the genome of influenza viruses and adenoviruses. CONCLUSION： The earthworm CTE displayed non-specific antiviral properties, possibly mediated by a combination of proteases, nucleases and lyso- zymes. Nucleases likely participate in the antiviral process, and degrade the genome of the virus thereby preventing further replication.展开更多
OBJECTIVE:This study screened serum tumor biomarkers by surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) to establish a subset which could be used for the prediction of Qi de...OBJECTIVE:This study screened serum tumor biomarkers by surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) to establish a subset which could be used for the prediction of Qi deficiency syndrome and phlegm and blood stasis in patients with non-small cell lung cancer;and as diagnostic model of Chinese medicine.METHODS:Serum samples from 63 lung cancer patients with Qi deficiency syndrome and phlegm and blood stasis,and 28 lung cancer patients with non-Qi deficiency syndrome and phlegm and blood stasis were analyzed using SELDI-TOF-MS with a PBS II-C protein chip reader.Protein profiles were generated using immobilized metal affinity capture(IMAC3) protein chips.Differentially-expressed proteins were screened.Protein peak clustering and classification analyses were performed using Biomarker Wizard and Biomarker Pattern software packages,respectively.RESULTS:A total of 268 effective protein peaks were detected in the 1,000-10,000 Da molecular range for the 15 serum proteins screened(P<0.05).The decision tree model was M 2284.97,with a sensitivity of 96.2% and a specificity of 66.7%.CONCLUSION:SELDI-TOF-MS techniques,combined with a decision tree model,can help identify serum proteomic biomarkers related to Qi deficiency syndrome and phlegm and blood stasis in lung cancer patients;and the predictive model can be used to discriminate between Chinese medicine diagnostic models of disease.展开更多
Inductive pulsed power supply(IPPS)is a promising type of power supplies for electromagnetic launch,but its advantage in energy density is strongly restricted by the primary power source.Fortunately,batteries become a...Inductive pulsed power supply(IPPS)is a promising type of power supplies for electromagnetic launch,but its advantage in energy density is strongly restricted by the primary power source.Fortunately,batteries become a suitable and practical choice for the primary source.In this paper,the working process of battery powering IPPS is analysed in detail.Various indicators of the charging performance are calculated,which are then proved to be irrelevant to most of the parameters of IPPS.Based on this,an optimization method for battery and IPPS system is proposed,in which the charging and discharging processes can be decoupled.And for the decoupled battery charging process,the bi-objective optimization model is suggested.Several examples illustrate the feasibility and effectiveness of the proposed optimization methods.In order to further improve the performance of the battery and IPPS system,the inherent time constant of the inductors and the output power density of the battery cell should be increased.展开更多
In order to ensure the safety of connected and automated vehicles(CAVs)threatened by cyberattack in the confluence area and mitigate the adverse impact of cyberattack propagation,a framework is built to depict the imp...In order to ensure the safety of connected and automated vehicles(CAVs)threatened by cyberattack in the confluence area and mitigate the adverse impact of cyberattack propagation,a framework is built to depict the impact of cyberattacks on traffic operation.Based on this framework,corresponding propagation suppression strategies are proposed for different types of cyberattacks in different periods.Under centralized control,game theory is used to solve the confluence sequence corresponding to the strategies.The results show that the proposed method can effectively inhibit the spread of cyberattacks on the premise of security.The initial control effect is the best.Compared with uncontrolled condition,in the 100 timesteps,11 susceptible vehicles are finally added,and the second is the immunity period,in which 10 susceptible vehicles were protected from cyberattack.Outbreak and latency control strategies also protect some vehicles.Under the control strategy of each stage,the peak value of infected vehicles and the duration of cyberattack are improved compared with the uncontrolled strategy.In addition,the traffic efficiency in the confluence area is also improved.This method can also be extended to such road types as diverging section,weaving section and intersection,so as to reduce the impact of cyberattacks on road network scale.展开更多
The insulating properties of silicone gel used for silicon carbide-insulated gate bipolar transistors encapsulation may deteriorate seriously under ultra-fast pulsed electric fields.The essence of insulation degradati...The insulating properties of silicone gel used for silicon carbide-insulated gate bipolar transistors encapsulation may deteriorate seriously under ultra-fast pulsed electric fields.The essence of insulation degradation lies in the deterioration of materials caused by dynamic phenomena at microscopic scale,such as charge trapping and detrapping.Different from the steady-state operating condition,insulating materials exhibit a sharp decrease in their insulating properties when subjected to a rapidly changing electric field.To investigate the insulation failure of silicone gel materials under an ultra-fast pulsed electric field,Marcus hopping mechanism for charge response is proposed.By calculating the relaxation time with different defects,we characterise the degradation of the materials.According to the force analysis of space charge,the authors establish a relationship between insulation failures and charge relaxation time.Combined with the experimental results on electrical treeing in silicone gel,the feasibility of the theory is verified.The experimental phenomenon can be well explained,that is,the initial voltage of the electrical trees decreased sharply with shortening the edge time of the pulsed electric field,especially on the nanosecond time scale.展开更多
基金supported by the Natural Science Foundation of Fujian Province, No. 2009J06018
文摘The Chinese herbs Herba Epimedii, Fructus Ligustri Lucidi and Rhizoma Polygonati were injected into Parkinson＇s disease mice established via intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine hydrochloride. The selective monoamine oxidase B inhibitor selegiline was used as a positive control drug. After successive administration for 4 weeks, Herba Epimedii could downregulate the expression of caspase-3 and increase the brain-derived neurotrophic factor level, as well as increase tyrosine hydroxylase activity in the substantia nigra of Parkinson＇s disease mouse models. Rhizoma Polygonaticould downregulate the expression of caspase-3 and FasL, and increase neural growth factor and brain-derived neurotrophic factor levels. Fructus Ligustn Lucidi could downregulate caspase-3 expression. Rhizoma Polygonati and Fructus Ligustn Lucidi did not produce obvious effects on tyrosine hydroxylase activity. Herba Epimedii and Fructus Ligustri Lucidi yielded similar effects on apoptosis-promoting factors to those elicited by selegiline. Herba Epimedii and Rhizoma Polygonati significantly increased the levels of neurotrophic factors compared with selegiline. Herba Epimedii significantly increased tyrosine hydroxylase activity compared with selegiline. It is indicated that the kidney-tonifying Chinese herbal preparation can downregulate the expression of apoptosis-promoting factors, increase neurotrophic factors levels in the substantia nigra and striatum, as well as increase tyrosine hydroxylase activity in the substantia nigra of Parkinson＇s disease mouse models, thereby exerting a stronger or similar neuroprotective effects compared with selegiline.
基金sponsored by the National Natural Science Foundation of China, No. 31140012, 31040056,31140079the Natural Science Foundation of Shanxi Province,No. 2006011113
文摘The present study involved a questionnaire survey of 156 mothers that gave birth to children with neural tube defects or had a history of pregnancy resulting in children with neural tube defects （case group） and 156 control mothers with concurrent healthy children （control group） as well as detection of mitochondrial membrane transporter protein gene [uncoupling protein 2 （UCP2）] polymorphism. The maternal UCP2 3＇ untranslated region （UTR） D/D genotype and D allele frequency were significantly higher in the case group compared with the control group （odds ratio （OR） 3.233; 95% confidence interval （C/） 1.103 9.476; P= 0.040; OR： 3.484; 95% CI： for neural tube defects 2.109 5.753; P 〈 0.001）. Univariate and multivariate logistic regression analysis of risk factors for neural tube defects showed that a matemal UCP2 3＇ UTR D/D genotype was negatively interacted with the mothers＇ consumption of frequent fresh fruit and vegetables （S = 0.007）, positively interacted with the mothers＇ frequency of germinated potato consumption （S = 2.15） and positively interacted with the mothers＇ body mass index （S = 3.50）. These findings suggest that maternal UCP2 3＇ UTR gene polymorphism, pregnancy time, consumption of germinated potatoes and body mass index are associated with an increased risk for neural tube defects in children from mothers living in Shanxi province, China. Moreover, there is an apparent gene-environment interaction involved in the development of neural tube defects in offspring.
基金the Water Conservancy Science and Technology Major Project of Hunan Province,China(Project XSKJ2019081-10)the China Scholarship Council(Grant No.202006370344)the First-class Project Special Funding of Yellow River Laboratory,China(Grant No.YRL22YL07).
文摘In the process of engineering construction such as tunnels and slopes,rock mass is frequently subjected to multiple levels of loading and unloading,while previous research ignores the impact of unloading rate on the stability of rock mass.A number of uniaxial multi-level cyclic loading-unloading experiments were conducted to better understand the effect of unloading rate on the deformation behavior,energy evolution,and damage properties of rock-like material.The experimental results demonstrated that the unloading rate and relative cyclic number clearly influence the deformation behavior and energy evo-lution of rock-like samples.In particular,as the relative cyclic number rises,the total strain and reversible strain both increase linearly,while the total energy density,elastic energy density,and dissipated energy density all rise nonlinearly.In contrast,the irreversible strain first decreases quickly,then stabilizes,and finally rises slowly.As the unloading rate increases,the total strain and reversible strain both increase,while the irreversible strain decreases.The dissipated energy damage was examined in light of the aforementioned experimental findings.The accuracy of the proposed damage model,which takes into account the impact of the unloading rate and relative cyclic number,is then confirmed by examining the consistency between the model predicted and the experimental results.The proposed damage model will make it easier to foresee how the multi-level loading-unloading cycles will affect the rock-like materials.
基金Supported by National TCM Project Application in the 11th Five-Year Period (NO. 2007BAI10B01-041)International Science Joint Project of the Ministry of Science and Technology of the People's Republic of China (No. 2008DFA32200)Natural Science Foundation of Fujian Province, China (No.2010J01197)
文摘OBJECTIVE： To investigate the distribution characteristics of TCM syndromes and the related herbal prescriptions for malignant tumors （MT）. METHODS： A clinical database of the TCM syndromes and the herbal prescriptions in treatment of 136 MT patients were established. The data were then analyzed using cluster and frequency analysis. RESULTS： According to the cluster analysis, the TCM syndromes in MT patients mainly included two patterns： deficiency of both Qi and Yin and internal accumulation of toxic heat. The commonlyprescribed herbs were Huangqi （Astraglus）, Nuizhenzi （Fructus Ligustri Lucidi）, Lingzhi （Ganoderma Lucidum）, Huaishan （Dioscorea Opposita）, Xiakucao （Prunella Vulgaris）, and Baihuasheshecao （Herba Hedyotidis）. CONCLUSION： Deficiency of Qi and Yin is the pri- mary syndrome of MT, and internal accumulation of toxic heat is the secondary syndrome. The herbs for Qi mainly heat-cl supplementation and Yin houris used, with earance and the assistance of detoxification. hment are herbs for
文摘OBJECTIVE： To test the in vitro antiviral activity of a crude tissue extract （CTE/from the earthworm Eisenia fetida, determine any effective components in the CTE, andelucidate possiblemechanismsofaction. METHODS： A CTE was made by homogenizing earthworms, followed by treatment with ammoni- um sulfate, then thermal denaturation. Inhibition of virus-induced cytopathic effect （CPE） was used to assess antiviral activity. Chromatographic analy- sis was used to identify effective components in the CTE. RESULTS： The CTE inhibited viral CPE at non-cyto- toxic concentrations. Chromatography indicated that antiviral components corresponded to three active peaks indicative of proteases, nucleases and lysozymes. For adenoviruses, reduction in viral ac- tivity occurred for 100 lag/mL CTE. The reduction in adenoviral activity for four fractions was 100%, 91.8%, 86.9%, and 94.7%. For influenza viruses, re- duction in viral activity of 100%, 86.6%, 69.1% and 88.3% was observed for 37 pg/mL CTE. In addition, three active fractions mixture had stronger antiviral activity （98.7% and 96.7%） than three fractions alone.Gel electrophoresis results indicated that nu- cleases from E. fetida could degrade the genome of influenza viruses and adenoviruses. CONCLUSION： The earthworm CTE displayed non-specific antiviral properties, possibly mediated by a combination of proteases, nucleases and lyso- zymes. Nucleases likely participate in the antiviral process, and degrade the genome of the virus thereby preventing further replication.
基金Supported by the National Natural Science Foundation of China(No.30572293)the "Eleventh Five" TCM Foundation for Major Clinical Research of PLA(No.2006051002)the Natural Science Foundation of Fujian Province,China(No. 2010J01197)
文摘OBJECTIVE:This study screened serum tumor biomarkers by surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) to establish a subset which could be used for the prediction of Qi deficiency syndrome and phlegm and blood stasis in patients with non-small cell lung cancer;and as diagnostic model of Chinese medicine.METHODS:Serum samples from 63 lung cancer patients with Qi deficiency syndrome and phlegm and blood stasis,and 28 lung cancer patients with non-Qi deficiency syndrome and phlegm and blood stasis were analyzed using SELDI-TOF-MS with a PBS II-C protein chip reader.Protein profiles were generated using immobilized metal affinity capture(IMAC3) protein chips.Differentially-expressed proteins were screened.Protein peak clustering and classification analyses were performed using Biomarker Wizard and Biomarker Pattern software packages,respectively.RESULTS:A total of 268 effective protein peaks were detected in the 1,000-10,000 Da molecular range for the 15 serum proteins screened(P<0.05).The decision tree model was M 2284.97,with a sensitivity of 96.2% and a specificity of 66.7%.CONCLUSION:SELDI-TOF-MS techniques,combined with a decision tree model,can help identify serum proteomic biomarkers related to Qi deficiency syndrome and phlegm and blood stasis in lung cancer patients;and the predictive model can be used to discriminate between Chinese medicine diagnostic models of disease.
基金National Natural Science Foundation of China,Grant/Award Number:61877063。
文摘Inductive pulsed power supply(IPPS)is a promising type of power supplies for electromagnetic launch,but its advantage in energy density is strongly restricted by the primary power source.Fortunately,batteries become a suitable and practical choice for the primary source.In this paper,the working process of battery powering IPPS is analysed in detail.Various indicators of the charging performance are calculated,which are then proved to be irrelevant to most of the parameters of IPPS.Based on this,an optimization method for battery and IPPS system is proposed,in which the charging and discharging processes can be decoupled.And for the decoupled battery charging process,the bi-objective optimization model is suggested.Several examples illustrate the feasibility and effectiveness of the proposed optimization methods.In order to further improve the performance of the battery and IPPS system,the inherent time constant of the inductors and the output power density of the battery cell should be increased.
基金supported by Key Research and Development Program of Shaanxi (Grant No.2023-YBGY-118)Scientific Research Project of Department of Transport of Shaanxi Province (Grant No.22-13X)。
文摘In order to ensure the safety of connected and automated vehicles(CAVs)threatened by cyberattack in the confluence area and mitigate the adverse impact of cyberattack propagation,a framework is built to depict the impact of cyberattacks on traffic operation.Based on this framework,corresponding propagation suppression strategies are proposed for different types of cyberattacks in different periods.Under centralized control,game theory is used to solve the confluence sequence corresponding to the strategies.The results show that the proposed method can effectively inhibit the spread of cyberattacks on the premise of security.The initial control effect is the best.Compared with uncontrolled condition,in the 100 timesteps,11 susceptible vehicles are finally added,and the second is the immunity period,in which 10 susceptible vehicles were protected from cyberattack.Outbreak and latency control strategies also protect some vehicles.Under the control strategy of each stage,the peak value of infected vehicles and the duration of cyberattack are improved compared with the uncontrolled strategy.In addition,the traffic efficiency in the confluence area is also improved.This method can also be extended to such road types as diverging section,weaving section and intersection,so as to reduce the impact of cyberattacks on road network scale.
基金Natural Science Foundation for Youth of Shandong Province,Grant/Award Number:ZR2023QE303China Postdoctoral Science Foundation,Grant/Award Number:2023M742055National Natural Science Foundation of China,Grant/Award Numbers:52277155,51907105。
文摘The insulating properties of silicone gel used for silicon carbide-insulated gate bipolar transistors encapsulation may deteriorate seriously under ultra-fast pulsed electric fields.The essence of insulation degradation lies in the deterioration of materials caused by dynamic phenomena at microscopic scale,such as charge trapping and detrapping.Different from the steady-state operating condition,insulating materials exhibit a sharp decrease in their insulating properties when subjected to a rapidly changing electric field.To investigate the insulation failure of silicone gel materials under an ultra-fast pulsed electric field,Marcus hopping mechanism for charge response is proposed.By calculating the relaxation time with different defects,we characterise the degradation of the materials.According to the force analysis of space charge,the authors establish a relationship between insulation failures and charge relaxation time.Combined with the experimental results on electrical treeing in silicone gel,the feasibility of the theory is verified.The experimental phenomenon can be well explained,that is,the initial voltage of the electrical trees decreased sharply with shortening the edge time of the pulsed electric field,especially on the nanosecond time scale.
