Loss of susceptibility to anoikis signals is a crucial step in metastasis.Anoikis resistance therefore represents a promising adjuvant therapeutic target for cancer management.In this study,we have conducted a rationa...Loss of susceptibility to anoikis signals is a crucial step in metastasis.Anoikis resistance therefore represents a promising adjuvant therapeutic target for cancer management.In this study,we have conducted a rationalized screening to search for novel leading anoikis sensitizer from daily foods.Among 19 tested dietary phytochemicals,the best results were obtained with apigenin,a natural component of celery.Phenotypically,apigenin sensitized breast cancer cells to anoikis,lowered the number of circulating tumor cells,and protected against breast cancer metastasis to lung in mice.Mechanistically,we demonstrated that the thromboxane A_(2)(TXA_(2))-TXA_(2)receptor(TP)axis has a critical role in acquired anoikis resistance by activating PI3K-Akt signaling pathway.Blockage of TXA_(2)signaling up-regulated p53 as well as its target gene p21,caused a G1 phase arrest,and finally led to apoptosis in breast cancer cells.TXA_(2)level was positively correlated with breast cancer cell anoikis rate,and apigenin significantly inhibited TXA_(2)biosynthesis in vitro and in vivo.Collectively,we identified apigenin as a potent anoikis sensitizer with anti-metastatic properties in a mouse model of breast cancer,and these findings might provide a rationale for introducing apigenin supplementation to breast cancer patients.展开更多
A microelectrode glucose biosensor based on a three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits.The nanostructure was fab...A microelectrode glucose biosensor based on a three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits.The nanostructure was fabricated by a two-step modification method on a microelectrode for loading a larger amount of glucose oxidase.The nanoporous structure was prepared on the surface of the platinum microelectrode by electrochemical etching,and then graphene oxide was deposited on the prepared nanoporous electrode by electrochemical deposition.The nanoporous platinum/graphene oxide nanostructure had the advantage of improving the effective surface area of the electrode and the loading quantity of glucose oxidase.As a result,the biosensor achieved a wide range of 0.1-20.0 mmol/L in glucose detection,which had the ability to accurately detect the glucose content.It was found that the three-dimensional hybrid nanostructure on the electrode surface realized the rapid direct electrochemistry of glucose oxidase.Therefore,the biosensor achieved high glucose detection sensitivity 11.64μA·L/(mmol.cm^(2)),low detection limit(13μmol/L)and rapid response time(reaching 95%steady-state response within 3 s),when calibrating in glucose standard solution.In agricultural application,the as-prepared biosensor was employed to detect the glucose concentration of tomato and cucumber samples.The results showed that the relative deviation of this method was less than 5%when compared with that of high-performance liquid chromatography,implying high accuracy of the presented biosensor in glucose detection in plants.展开更多
基金supported by the National Natural Science Foundation of China (81773064,31972973,32021005)National Youth 1000 Talents Plan+2 种基金the Jiangsu Specially-Appointed Professor ProgramJiangsu Province Recruitment Plan for High-level,Innovative and Entrepreneurial Talents (Innovative Research Team)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
文摘Loss of susceptibility to anoikis signals is a crucial step in metastasis.Anoikis resistance therefore represents a promising adjuvant therapeutic target for cancer management.In this study,we have conducted a rationalized screening to search for novel leading anoikis sensitizer from daily foods.Among 19 tested dietary phytochemicals,the best results were obtained with apigenin,a natural component of celery.Phenotypically,apigenin sensitized breast cancer cells to anoikis,lowered the number of circulating tumor cells,and protected against breast cancer metastasis to lung in mice.Mechanistically,we demonstrated that the thromboxane A_(2)(TXA_(2))-TXA_(2)receptor(TP)axis has a critical role in acquired anoikis resistance by activating PI3K-Akt signaling pathway.Blockage of TXA_(2)signaling up-regulated p53 as well as its target gene p21,caused a G1 phase arrest,and finally led to apoptosis in breast cancer cells.TXA_(2)level was positively correlated with breast cancer cell anoikis rate,and apigenin significantly inhibited TXA_(2)biosynthesis in vitro and in vivo.Collectively,we identified apigenin as a potent anoikis sensitizer with anti-metastatic properties in a mouse model of breast cancer,and these findings might provide a rationale for introducing apigenin supplementation to breast cancer patients.
基金funded by the Key-Area Research and Development Program of Guangdong Province (2019B020219002)the Characteristic Innovation Project of Ordinary University of Guangdong Province (2019KTSCX018)+1 种基金the Guangdong Natural Science Funds for Distinguished Young Scholar (2014A030306005)the Guangdong Basic and Applied Basic Research Foundation (2019A1515110929),China.
文摘A microelectrode glucose biosensor based on a three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits.The nanostructure was fabricated by a two-step modification method on a microelectrode for loading a larger amount of glucose oxidase.The nanoporous structure was prepared on the surface of the platinum microelectrode by electrochemical etching,and then graphene oxide was deposited on the prepared nanoporous electrode by electrochemical deposition.The nanoporous platinum/graphene oxide nanostructure had the advantage of improving the effective surface area of the electrode and the loading quantity of glucose oxidase.As a result,the biosensor achieved a wide range of 0.1-20.0 mmol/L in glucose detection,which had the ability to accurately detect the glucose content.It was found that the three-dimensional hybrid nanostructure on the electrode surface realized the rapid direct electrochemistry of glucose oxidase.Therefore,the biosensor achieved high glucose detection sensitivity 11.64μA·L/(mmol.cm^(2)),low detection limit(13μmol/L)and rapid response time(reaching 95%steady-state response within 3 s),when calibrating in glucose standard solution.In agricultural application,the as-prepared biosensor was employed to detect the glucose concentration of tomato and cucumber samples.The results showed that the relative deviation of this method was less than 5%when compared with that of high-performance liquid chromatography,implying high accuracy of the presented biosensor in glucose detection in plants.
