Asian leaf-litter toads of the genus Leptobrachella represent a great anuran diversification in Asia.Previous studies have suggested that the diversity of this genus is still underestimated. During herpetological surv...Asian leaf-litter toads of the genus Leptobrachella represent a great anuran diversification in Asia.Previous studies have suggested that the diversity of this genus is still underestimated. During herpetological surveys from 2013 to 2018, a series of Leptobrachella specimens were collected from the international border areas in the southern and western parts of Yunnan Province, China.Subsequent analyses based on morphological and molecular data revealed three distinct and previously unknown lineages, which we formally describe as three new species herein. Among them, we describe a new species that occurs at the highest known elevation for Leptobrachella in China. Four species of Leptobrachella, including two new species, are found in the same reserve. Furthermore, our results suggest that the population from Longchuan County,Yunnan, may represent an additional new species of Leptobrachella, although we tentatively assigned it to Leptobrachella cf. yingjiangensis due to the small sample size examined. Lastly, we provide the first description of females of L. yingjiangensis. Our results further highlight that both micro-endemism and sympatric distributions of species are common patterns in Leptobrachella, that contribute to taxonomic and conservation challenges in these frogs. We provide an identification key for Leptobrachella known to occur in Yunnan. Given the lack of knowledge on species diversity of Leptobrachella along international border areas, we recommend that future studies include transboundary collaborative surveys.展开更多
Evolutionary biologists are always interested in deciphering the geographic context of diversification,therefore they introduced the concept of comparative phylogeography, which helps to identify common mechanisms tha...Evolutionary biologists are always interested in deciphering the geographic context of diversification,therefore they introduced the concept of comparative phylogeography, which helps to identify common mechanisms that contribute to shared genetic structures among organisms from the same region.Here, we used multi-locus genetic data along with environmental data to investigate shared phylogeographic patterns among three Asianendemic newt genera, Cynops, Paramesotriton and Pachytriton, which occurred in montane/submontane streams or ponds in southern China. Our 222samples from 78 localities covered the entire range of the three genera and represented the largest dataset of this group to date. We reconstructed matrilineal genealogies from two protein-coding,mitochondrial genes, and gene network from two nuclear genes. We also estimated divergence times of major cladogenetic events and used occurrence data to evaluate niche difference and similarity between lineages. Our results revealed a common basal split in all three genera that corresponds to the separation of two geographic terrains of southern China.Those ancient divergence occurred during middle to late Miocene and likely correlate with paleoclimatic fluctuations caused by the uplift of the Qinghai-Xizang (Tibet) Plateau (QTP).Particularly,the strengthening and weakening of Asian summer monsoons during the Miocene may have profoundly impacted southern China and led to repeatedly vicariance in those newts.However,despite differences in realized niches between lineages,there is no evidence for divergence of fundamental niches.Preservation of old newt matriline lineages in mountains of southern China suggests that the region acts as both museums and cradles of speciation.Based on those results,we advocate a multi-pronged protection strategy for newts in the three genera.展开更多
AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we de...AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we detected expression of lysosome-associated membrane protein 2a(Lamp-2a), which is the key protein of CMA, by western blot in Hep G2 and SMMC7721 cells after irradiation. We further used sh RNA Lamp-2a HCC cells to verify the radioresistance induced by CMA. Next, we detected the HMGB1 and p53 expression after irradiation by western blot, and we further used RNA interference and ethyl pyruvate(EP), as a HMGB1 inhibitor, to observe changes of p53 expression. Finally, an immunoprecipitation assay was conducted to explore the interaction between Lamp-2a and HMGB1, and the data were analyzed. RESULTS We found the expression of Lamp-2a was increased on irradiation while apoptosis decreased in Hep G2 and SMMC7721 cells. The apoptosis was increased markedly in the sh RNA Lamp-2a Hep G2 and SMMC7721 cells as detected by western blot and colony formation assay. Next, we found p53 expression was gradually reduced on irradiation but obviously increased in sh RNA Lamp-2a cells. Furthermore, p53 increased the cell apoptosis on irradiation in Hep3B(p53-/-) cells. Finally, p53 levels were regulated by HMGB1 as measured through RNA interference and the EP treatment. HMGB1 was able to combine with Lamp-2a as seen by immunoprecipitation assay and was degraded via the CMA pathway. The decreased HMGB1 inhibited p53 expression induced by irradiation and further reduced the apoptosis in HCC cells. CONCLUSION CMA pathway activation appears to down-regulate the susceptibility of HCC to irradiation by degrading HMGB1 with further impact on p53 expression. These findings have clinical relevance for radiotherapy of HCC.展开更多
We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed th...We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed three distinct lineages,namely the newly described Hebius weixiensis sp.nov.,as well as H.octolineatus(Boulenger,1904),and H.septemlineatus comb.nov.(Schmidt 1925),which is removed from synonymy with H.octolineatus.Based on mitochondrial genealogy,Hebius weixiensis sp.nov.is sister to H.septemlineatus comb.nov.,while H.octolineatus is sister to H.bitaeniatus.The new species and H.septemlineatus comb.展开更多
We describe a new species of frog in the dicroglossid genus Fejervarya from Ban Monjong, Omkoi District, Chiang Mai Province, northern Thailand. Analysis of DNA sequence data from the mitochondrial gene 16S, advertise...We describe a new species of frog in the dicroglossid genus Fejervarya from Ban Monjong, Omkoi District, Chiang Mai Province, northern Thailand. Analysis of DNA sequence data from the mitochondrial gene 16S, advertisement calls, and morphological distinctiveness support recognition of the new species. Matrilineal genealogy suggests that the new population from Chiang Mai is a sister taxon to the South Asian clade that includes F. syhadrensis, F. granosa, and F. pierreL The new species, Fejervarya chiangmaiensis sp. nov., differs morphologically from its congeners by its relatively small body size and proportions and the presence of dorsal warts and dermal ridges. Discovery of this new species indicates that the biodiversity of amphibians in this region remains underestimated.展开更多
We describe a new species, Fejervarya muangkanensis sp. nov., based on a series of specimens collected from Ban Tha Khanun, Thong Pha Phum District, Kanchanaburi Province, Thailand. The new species is easily distingui...We describe a new species, Fejervarya muangkanensis sp. nov., based on a series of specimens collected from Ban Tha Khanun, Thong Pha Phum District, Kanchanaburi Province, Thailand. The new species is easily distinguished from its congeners by morphological and molecular data, and can be diagnosed by the following characters： （1） small size （adult male snout-vent length （SVL） 33.5 mm; female SVL 40.0-40.9 mm）; （2） tympanum small, discernible but unclear; （3） poorly developed toe webbing; （4） no lateral line system in adults; （5） characteristic ＂Fejervaryan＂ lines present in females; and （6） femoral glands absent. Molecular phylogenetic analysis of mitochondrial 16S rRNA further supports it as a distinct lineage and distinguishes it from its congeners for which sequences are available.展开更多
DEAR EDITOR,Based upon morphological and molecular evidence,the authors revised the genus Rohanixalus Biju,Garg,Gokulakrishnan,Chandrakasan,Thammachoti,Ren,Gopika,Bisht,Hamidy and Shouche,2020(Anura:Rhacophoridae)in C...DEAR EDITOR,Based upon morphological and molecular evidence,the authors revised the genus Rohanixalus Biju,Garg,Gokulakrishnan,Chandrakasan,Thammachoti,Ren,Gopika,Bisht,Hamidy and Shouche,2020(Anura:Rhacophoridae)in China through describing one new species,adding one species to the fauna(R.shyamrupus)and supplementing data on one species(Rohanixalus hansenae;Supplementary Materials).展开更多
OBJECTIVE To identify risk factors for relapse and death in patients with T1 to T2 breast cancer with 0-3 positive axillary lymph nodes.METHODS The case files of 540 breast cancer patients with T1-T2 tumors with 0-3 p...OBJECTIVE To identify risk factors for relapse and death in patients with T1 to T2 breast cancer with 0-3 positive axillary lymph nodes.METHODS The case files of 540 breast cancer patients with T1-T2 tumors with 0-3 positive nodes were reviewed retrospectively. Ten-year locoregional recurrence （LRR）, distant recurrence （DR）, disease-free survival （DFS） and overall survival （OS） of the patients were analyzed. Univariate statistical analysis and Cox proportional hazards models were carried out with SPSS so ware v.16.0.RESULTS The median follow-up of all the patients was 7.2 years. On multivariate analysis, 〉 20% positive axillary nodes was the only variable that influenced LRR adversely （hazard ratio[HR], 12.816; 95% confidence interval, 4.657-35.266, P 〈 0.001）; 〉 20% positive axillary nodes and ductal carcinoma were variables that influenced DR adversely （HR, 11.088, 95% confidence interval, 3.807-32.297, P 〈 0.001; HR, 0.390, 95% confidence interval, 0.179-0.851, P = 0.018）; 1-3 positive axillary nodes and 〉 20% positive axillary nodes were the only variables that had negative e. ect on 10-year OS （HR, 2.110, 95% confi dence interval, 1.364-3.264, P = 0.001; HR, 10.244, 95% confidence interval, 3.497-30.011, P 〈 0.001） and they were also adverse prognostic variables on 10-year DFS （HR, 1.634, 95% confidence interval, 1.171-2.279, P = 0.004; HR, 7.339, 95% confi dence interval,2.906-18.530, P 〈 0.001）.CONCLUSION Axillary lymph nodal status is the only risk factor with a signifi cant impact on 10-year LRR, DR, OS and DFS.Patients with T1-T2 breast cancer with 0-3 positive lymph nodes have the LRR and DR of over 10 years, and the OS and DFS of less than 10 years, compared to patients with negative lymph nodes.Histology in primary tumors is a signifi cant prognostic factor for the 10-year DR.展开更多
South China and Indochina host striking species diversity and endemism. Complex tectonic and climatic evolutions appear to be the main drivers of the biogeographic patterns. In this study, based on the geologic histor...South China and Indochina host striking species diversity and endemism. Complex tectonic and climatic evolutions appear to be the main drivers of the biogeographic patterns. In this study, based on the geologic history of this region, we test 2 hypotheses using the evolutionary history of Microhyla fissipes species complex. Using DNA sequence data from both mitochondrial and nuclear genes, we first test the hypothesis that the Red River is a barrier to gene flow and dispersal. Second, we test the hypothesis that Pleistocene climatic cycling affected the genetic structure and population history of these frogs. We detect 2 major genetic splits that associate with the Red River. Time estimation suggests that late Miocene tectonic movement associated with the Red River drove their diversification. Species distribution modeling （SDM） resolves significant ecolo- gical differences between sides of the Red River. Thus, ecological divergence also probably promoted and maintained the diversification. Genogeography, historical demography, and SDM associate patterns in southern China with climate changes of the last glacial maximum （LGM）, but not Indochina. Differences in geography and climate between the 2 areas best explain the discovery. Responses to the Pleistocene glacial-interglacial cycling vary among species and regions.展开更多
基金the National Natural Science Foundation of China(31900323 to J.M.C.,31622052 to J.C.)Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(CAS)(Y4ZK111B01:2017CASSEABRIQG002)Nanjing Institute of Environmental Sciences,Ministry of Environmental Protection of China,and the Animal Branch of the Germplasm Bank of Wild Species,CAS(Large Research Infrastructure Funding)to J.C.,Russian Science Foundation(19-14-00050)to N.A.P.,Biodiversity Investigation,Observation and Assessment Program(2019-2023)of Ministry of Ecology and Environment of China to Z.Y.Y.,and Unit of Excellence 2020 on Biodiversity and Natural Resources Management,University of Phayao to C.S.
文摘Asian leaf-litter toads of the genus Leptobrachella represent a great anuran diversification in Asia.Previous studies have suggested that the diversity of this genus is still underestimated. During herpetological surveys from 2013 to 2018, a series of Leptobrachella specimens were collected from the international border areas in the southern and western parts of Yunnan Province, China.Subsequent analyses based on morphological and molecular data revealed three distinct and previously unknown lineages, which we formally describe as three new species herein. Among them, we describe a new species that occurs at the highest known elevation for Leptobrachella in China. Four species of Leptobrachella, including two new species, are found in the same reserve. Furthermore, our results suggest that the population from Longchuan County,Yunnan, may represent an additional new species of Leptobrachella, although we tentatively assigned it to Leptobrachella cf. yingjiangensis due to the small sample size examined. Lastly, we provide the first description of females of L. yingjiangensis. Our results further highlight that both micro-endemism and sympatric distributions of species are common patterns in Leptobrachella, that contribute to taxonomic and conservation challenges in these frogs. We provide an identification key for Leptobrachella known to occur in Yunnan. Given the lack of knowledge on species diversity of Leptobrachella along international border areas, we recommend that future studies include transboundary collaborative surveys.
基金supported by the Strategic Priority Research Program,CAS (XDB31040202,XDA19050303)China’s Biodiversity Observation Network (Sino-BON),Digitalization,Development and Application of Biotic Resource (202002AA100007)+5 种基金Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of Sciences (Large Research Infrastructure Funding)National Natural Science Foundation of China (32170478)Yunnan FundamentalResearchProject(202001AW070016,202005AC160046)Young Talent Project of China Association for Science and Technology (2019-2021QNRC001)Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0501)supported by the CAS President’s International Fellowship Initiative (PIFI) to R.W.M.
文摘Evolutionary biologists are always interested in deciphering the geographic context of diversification,therefore they introduced the concept of comparative phylogeography, which helps to identify common mechanisms that contribute to shared genetic structures among organisms from the same region.Here, we used multi-locus genetic data along with environmental data to investigate shared phylogeographic patterns among three Asianendemic newt genera, Cynops, Paramesotriton and Pachytriton, which occurred in montane/submontane streams or ponds in southern China. Our 222samples from 78 localities covered the entire range of the three genera and represented the largest dataset of this group to date. We reconstructed matrilineal genealogies from two protein-coding,mitochondrial genes, and gene network from two nuclear genes. We also estimated divergence times of major cladogenetic events and used occurrence data to evaluate niche difference and similarity between lineages. Our results revealed a common basal split in all three genera that corresponds to the separation of two geographic terrains of southern China.Those ancient divergence occurred during middle to late Miocene and likely correlate with paleoclimatic fluctuations caused by the uplift of the Qinghai-Xizang (Tibet) Plateau (QTP).Particularly,the strengthening and weakening of Asian summer monsoons during the Miocene may have profoundly impacted southern China and led to repeatedly vicariance in those newts.However,despite differences in realized niches between lineages,there is no evidence for divergence of fundamental niches.Preservation of old newt matriline lineages in mountains of southern China suggests that the region acts as both museums and cradles of speciation.Based on those results,we advocate a multi-pronged protection strategy for newts in the three genera.
基金Supported by Natural Science Foundation of Hebei Province,China,No.H2016209007
文摘AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we detected expression of lysosome-associated membrane protein 2a(Lamp-2a), which is the key protein of CMA, by western blot in Hep G2 and SMMC7721 cells after irradiation. We further used sh RNA Lamp-2a HCC cells to verify the radioresistance induced by CMA. Next, we detected the HMGB1 and p53 expression after irradiation by western blot, and we further used RNA interference and ethyl pyruvate(EP), as a HMGB1 inhibitor, to observe changes of p53 expression. Finally, an immunoprecipitation assay was conducted to explore the interaction between Lamp-2a and HMGB1, and the data were analyzed. RESULTS We found the expression of Lamp-2a was increased on irradiation while apoptosis decreased in Hep G2 and SMMC7721 cells. The apoptosis was increased markedly in the sh RNA Lamp-2a Hep G2 and SMMC7721 cells as detected by western blot and colony formation assay. Next, we found p53 expression was gradually reduced on irradiation but obviously increased in sh RNA Lamp-2a cells. Furthermore, p53 increased the cell apoptosis on irradiation in Hep3B(p53-/-) cells. Finally, p53 levels were regulated by HMGB1 as measured through RNA interference and the EP treatment. HMGB1 was able to combine with Lamp-2a as seen by immunoprecipitation assay and was degraded via the CMA pathway. The decreased HMGB1 inhibited p53 expression induced by irradiation and further reduced the apoptosis in HCC cells. CONCLUSION CMA pathway activation appears to down-regulate the susceptibility of HCC to irradiation by degrading HMGB1 with further impact on p53 expression. These findings have clinical relevance for radiotherapy of HCC.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)Program(2019QZKK0501)Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment,China(2019HJ2096001006)+4 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)(XDA20050201)Digitalization,Development and Application of Biotic Resource(202002AA100007)Sciences and Technology Department of Sichuan Province(2020YFSY0033)State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,CAS(GREKF 19-02)Animal Branch of the Germplasm Bank of Wild Species,CAS(Large Research Infrastructure Funding)。
文摘We describe a new species of the genus Hebius and provide evidence for the validity of H.septemlineatus comb.nov..Morphological and molecular analyses of Hebius specimens collected in Yunnan Province,China,revealed three distinct lineages,namely the newly described Hebius weixiensis sp.nov.,as well as H.octolineatus(Boulenger,1904),and H.septemlineatus comb.nov.(Schmidt 1925),which is removed from synonymy with H.octolineatus.Based on mitochondrial genealogy,Hebius weixiensis sp.nov.is sister to H.septemlineatus comb.nov.,while H.octolineatus is sister to H.bitaeniatus.The new species and H.septemlineatus comb.
基金supported by the Program of the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01:2015CASEABRI002)National Natural Science Foundation of China(31501843)+5 种基金Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of Sciences(Large Research Infrastructure Funding)sponsored by the Chinese Academy of Sciences Visiting Fellowship for Researchers(Postdoc.) from Developing Countries(2013FFS130015)supported by the Russian Science Foundation(RSF grant No.14-50-00029)supported by a NSERC Discovery Grant(3148)the ROM Foundationthe ROM Members Volunteer Committee
文摘We describe a new species of frog in the dicroglossid genus Fejervarya from Ban Monjong, Omkoi District, Chiang Mai Province, northern Thailand. Analysis of DNA sequence data from the mitochondrial gene 16S, advertisement calls, and morphological distinctiveness support recognition of the new species. Matrilineal genealogy suggests that the new population from Chiang Mai is a sister taxon to the South Asian clade that includes F. syhadrensis, F. granosa, and F. pierreL The new species, Fejervarya chiangmaiensis sp. nov., differs morphologically from its congeners by its relatively small body size and proportions and the presence of dorsal warts and dermal ridges. Discovery of this new species indicates that the biodiversity of amphibians in this region remains underestimated.
基金supported by the National Natural Science Foundation of China(31501843)Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(CAS)(Y4ZK111B01:2015CASEABRI002)Youth Innovation Promotion Association,CAS,and Animal Branch of the Germplasm Bank of Wild Species,CAS(Large Research Infrastructure Funding)
文摘We describe a new species, Fejervarya muangkanensis sp. nov., based on a series of specimens collected from Ban Tha Khanun, Thong Pha Phum District, Kanchanaburi Province, Thailand. The new species is easily distinguished from its congeners by morphological and molecular data, and can be diagnosed by the following characters： （1） small size （adult male snout-vent length （SVL） 33.5 mm; female SVL 40.0-40.9 mm）; （2） tympanum small, discernible but unclear; （3） poorly developed toe webbing; （4） no lateral line system in adults; （5） characteristic ＂Fejervaryan＂ lines present in females; and （6） femoral glands absent. Molecular phylogenetic analysis of mitochondrial 16S rRNA further supports it as a distinct lineage and distinguishes it from its congeners for which sequences are available.
基金supported by the Fundamental Research Fund for Central Universities (SWU-KR22014)National Natural Science Foundation of China (NSFC32170478,32370478)+8 种基金Yunnan Fundamental Research Project (202001AW070016,202005AC160046)“Special Fund for Youth Team of Southwest University” (SWU-XJPY202302)to Y.Z.Y.National Key R&D Program of China (2022YFC2602500)the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (2019QZKK0501)Survey of Wildlife Resources in Key Areas of Xizang (ZL202203601)China’s Biodiversity Observation Network (Sino-BON)Animal Branch of Germplasm Bank of Wild Species,Chinese Academy of Sciences (Large Research Infrastructure Fund)to J.C.Unit of Excellence 2024 on Integrative diversity assessment of aquatic animals from Thailand (Fundamental FundFF67)to C.S。
文摘DEAR EDITOR,Based upon morphological and molecular evidence,the authors revised the genus Rohanixalus Biju,Garg,Gokulakrishnan,Chandrakasan,Thammachoti,Ren,Gopika,Bisht,Hamidy and Shouche,2020(Anura:Rhacophoridae)in China through describing one new species,adding one species to the fauna(R.shyamrupus)and supplementing data on one species(Rohanixalus hansenae;Supplementary Materials).
文摘OBJECTIVE To identify risk factors for relapse and death in patients with T1 to T2 breast cancer with 0-3 positive axillary lymph nodes.METHODS The case files of 540 breast cancer patients with T1-T2 tumors with 0-3 positive nodes were reviewed retrospectively. Ten-year locoregional recurrence （LRR）, distant recurrence （DR）, disease-free survival （DFS） and overall survival （OS） of the patients were analyzed. Univariate statistical analysis and Cox proportional hazards models were carried out with SPSS so ware v.16.0.RESULTS The median follow-up of all the patients was 7.2 years. On multivariate analysis, 〉 20% positive axillary nodes was the only variable that influenced LRR adversely （hazard ratio[HR], 12.816; 95% confidence interval, 4.657-35.266, P 〈 0.001）; 〉 20% positive axillary nodes and ductal carcinoma were variables that influenced DR adversely （HR, 11.088, 95% confidence interval, 3.807-32.297, P 〈 0.001; HR, 0.390, 95% confidence interval, 0.179-0.851, P = 0.018）; 1-3 positive axillary nodes and 〉 20% positive axillary nodes were the only variables that had negative e. ect on 10-year OS （HR, 2.110, 95% confi dence interval, 1.364-3.264, P = 0.001; HR, 10.244, 95% confidence interval, 3.497-30.011, P 〈 0.001） and they were also adverse prognostic variables on 10-year DFS （HR, 1.634, 95% confidence interval, 1.171-2.279, P = 0.004; HR, 7.339, 95% confi dence interval,2.906-18.530, P 〈 0.001）.CONCLUSION Axillary lymph nodal status is the only risk factor with a signifi cant impact on 10-year LRR, DR, OS and DFS.Patients with T1-T2 breast cancer with 0-3 positive lymph nodes have the LRR and DR of over 10 years, and the OS and DFS of less than 10 years, compared to patients with negative lymph nodes.Histology in primary tumors is a signifi cant prognostic factor for the 10-year DR.
基金Acknowledgments We thank Sheng-guo Fang, Hal-tan Shi, Li-jun Wang, Li-min Ding, Yun-yu Wang, Ban-ling Zhang, Hai-peng Zhao, Jin-ming Chen, Jun-xiao Yang, Jia-jun Zhou, Gang Yao, Nikolai L. Orlov, Son Nguyen Truong, Tao Thien Nguyen, Truong Quang Nguyen, Tran Ngoc Ninh, Ba Dinh Vo, Andrei N. Kuznetsov, Leonid P. Korzoun, Anna B. Vassilieva, Eduard A. Galoyan, Igor V. Palko, Suwat Seaya, and Prakayjit Saengham for helping with sample col- lections. We thank the local Forestry Department and National reserve helped for the fieldworks in China. We thank the Forestry Department of Thailand and Vietnam for permissions to undertake field survey, specimen collections and local national park for helping. The Institute of Animal for Scientific Purposes Development （IAD） issued permission （No.U1-01205-2558） in Thailand. The Department of Forestry, Ministry of Agriculture and Rural Development of Vietnam issued permission （permits No. 170/TCLN-BTI＇N, 982/TCLN-BTTN and 831/TCLN-BTTN） in Vietnam. For loans of tissues, we thank Amy Lathrop from the Royal Ontario Museum （ROM）, Toronto, Canada. We gratefully acknowledge Wei-wei Zhou and Yong-jie Wu for providing thoughtful comments during preparation of the previous version of this manuscript.This work was supported by the program of Chinese Academy of Sciences （2015CASEABRI002）, the Ministry of Science and Technology of China （2011FY120200）, the National Natural Science Foundation of China （31090250）, and the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences （the Large Research Infrastructure Funding） to J. C. the Chinese Academy of Sciences Visiting Fellowship for Researchers （Postdoc） from Developing Countries （2013FFS130015） to C. S.+2 种基金 the National Natural Science Foundation of China （31501843） to H. M. C. the Russian Foundation of Basic Research （Grant No. RFBR Taiwan No. 14-04- 92000, 15-29-02771 and 15-04-02029） and the Russian Science Foundation （RSF grant No 14-50-00029） to N. A. P. NSERC Discovery Grant （3148）, the ROM Foundation, and the ROM Members Volunteer Committee to R. W. M.
文摘South China and Indochina host striking species diversity and endemism. Complex tectonic and climatic evolutions appear to be the main drivers of the biogeographic patterns. In this study, based on the geologic history of this region, we test 2 hypotheses using the evolutionary history of Microhyla fissipes species complex. Using DNA sequence data from both mitochondrial and nuclear genes, we first test the hypothesis that the Red River is a barrier to gene flow and dispersal. Second, we test the hypothesis that Pleistocene climatic cycling affected the genetic structure and population history of these frogs. We detect 2 major genetic splits that associate with the Red River. Time estimation suggests that late Miocene tectonic movement associated with the Red River drove their diversification. Species distribution modeling （SDM） resolves significant ecolo- gical differences between sides of the Red River. Thus, ecological divergence also probably promoted and maintained the diversification. Genogeography, historical demography, and SDM associate patterns in southern China with climate changes of the last glacial maximum （LGM）, but not Indochina. Differences in geography and climate between the 2 areas best explain the discovery. Responses to the Pleistocene glacial-interglacial cycling vary among species and regions.
