Cryo-electron microscopy(cryo-EM) provides a powerful tool to resolve the structure of biological macromolecules in natural state. One advantage of cryo-EM technology is that different conformation states of a protein...Cryo-electron microscopy(cryo-EM) provides a powerful tool to resolve the structure of biological macromolecules in natural state. One advantage of cryo-EM technology is that different conformation states of a protein complex structure can be simultaneously built, and the distribution of different states can be measured. This provides a tool to push cryo-EM technology beyond just to resolve protein structures, but to obtain the thermodynamic properties of protein machines. Here, we used a deep manifold learning framework to get the conformational landscape of Kai C proteins, and further obtained the thermodynamic properties of this central oscillator component in the circadian clock by means of statistical physics.展开更多
This paper proposes a deterministic two-stage mixed integer linear programming(TSMILP)approach to solve the reserve constrained dynamic economic dispatch(DED)problem considering valve-point effect(VPE).In stage one,th...This paper proposes a deterministic two-stage mixed integer linear programming(TSMILP)approach to solve the reserve constrained dynamic economic dispatch(DED)problem considering valve-point effect(VPE).In stage one,the nonsmooth cost function and the transmission loss are piecewise linearized and consequently the DED problem is formulated as a mixed integer linear programming(MILP)problem,which can be solved by commercial solvers.In stage two,based on the solution obtained in stage one,a range compression technique is proposed to make a further exploitation in the subspace of the whole solution domain.Due to the linear approximation of the transmission loss,the solution obtained in stage two dose not strictly satisfies the power balance constraint.Hence,a forward procedure is employed to eliminate the error.The simulation results on four test systems show that TSMILP makes satisfactory performances,in comparison with the existing methods.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 12090054)。
文摘Cryo-electron microscopy(cryo-EM) provides a powerful tool to resolve the structure of biological macromolecules in natural state. One advantage of cryo-EM technology is that different conformation states of a protein complex structure can be simultaneously built, and the distribution of different states can be measured. This provides a tool to push cryo-EM technology beyond just to resolve protein structures, but to obtain the thermodynamic properties of protein machines. Here, we used a deep manifold learning framework to get the conformational landscape of Kai C proteins, and further obtained the thermodynamic properties of this central oscillator component in the circadian clock by means of statistical physics.
基金supported by Guangdong Yudean Group Co.LTD,Guangzhou 510630,China.
文摘This paper proposes a deterministic two-stage mixed integer linear programming(TSMILP)approach to solve the reserve constrained dynamic economic dispatch(DED)problem considering valve-point effect(VPE).In stage one,the nonsmooth cost function and the transmission loss are piecewise linearized and consequently the DED problem is formulated as a mixed integer linear programming(MILP)problem,which can be solved by commercial solvers.In stage two,based on the solution obtained in stage one,a range compression technique is proposed to make a further exploitation in the subspace of the whole solution domain.Due to the linear approximation of the transmission loss,the solution obtained in stage two dose not strictly satisfies the power balance constraint.Hence,a forward procedure is employed to eliminate the error.The simulation results on four test systems show that TSMILP makes satisfactory performances,in comparison with the existing methods.
