Recently,there has been a continuous stream of reports on emerging tick-borne pathogens affecting humans.Qinghai Province,located in the northweastern region,is one of China’s major pastoral areas,providing a suitabl...Recently,there has been a continuous stream of reports on emerging tick-borne pathogens affecting humans.Qinghai Province,located in the northweastern region,is one of China’s major pastoral areas,providing a suitable environment for ticks''survival and transmitting tick-borne pathogens.Here,we collected 560 free-living and parasitic ticks from 11 locations in Qinghai Province using the flag-drag method or tweezers,identifying them as belonging to 4 species of ticks.The overall positivity rate for tick-borne pathogens was 51.61%,comprisingRickettsia(34.64%),Anaplasma(5.00%),Ehrlichia(2.14%),Borrelia burgdorferi sensu lato(BBSL)(7.50%),Babesia(0.18%),andTheileria(5.89%).Sequencing revealed the presence of 7 species ofRickettsia,4 species ofAnaplasma,2 species ofEhrlichia,2 species of BBSL,1 species ofBabesia,and 3 species ofTheileria.Among the ticks,6.43%were co-infected with 2 pathogens,while 0.36%exhibited co-infection with 3 pathogens.Significant correlations(P<0.05)were observed between the prevalence of tick-borne pathogens and factors including tick species,sex,developmental stages,parasitic status,and blood-feeding status.The results highlight the diverse distribution of tick-borne pathogens in Qinghai Province,posing a significant threat to both local animal husbandry and human health.It underscores the need to enhance systematic monitoring of tick-borne pathogens in the local population and livestock.展开更多
基金funded by the National Natural Science Foundation of China(U2002219)The datasets generated or analyzed during the current study are available from the corresponding author on reasonable requestAll the nucleotide sequences obtained in this study are available in the GenBank of National Center for Biotechnology Information with accession numbers OR805110-OR805127,OR792783-OR792797,OR793990-OR793998,and OR813910-OR813915.
文摘Recently,there has been a continuous stream of reports on emerging tick-borne pathogens affecting humans.Qinghai Province,located in the northweastern region,is one of China’s major pastoral areas,providing a suitable environment for ticks''survival and transmitting tick-borne pathogens.Here,we collected 560 free-living and parasitic ticks from 11 locations in Qinghai Province using the flag-drag method or tweezers,identifying them as belonging to 4 species of ticks.The overall positivity rate for tick-borne pathogens was 51.61%,comprisingRickettsia(34.64%),Anaplasma(5.00%),Ehrlichia(2.14%),Borrelia burgdorferi sensu lato(BBSL)(7.50%),Babesia(0.18%),andTheileria(5.89%).Sequencing revealed the presence of 7 species ofRickettsia,4 species ofAnaplasma,2 species ofEhrlichia,2 species of BBSL,1 species ofBabesia,and 3 species ofTheileria.Among the ticks,6.43%were co-infected with 2 pathogens,while 0.36%exhibited co-infection with 3 pathogens.Significant correlations(P<0.05)were observed between the prevalence of tick-borne pathogens and factors including tick species,sex,developmental stages,parasitic status,and blood-feeding status.The results highlight the diverse distribution of tick-borne pathogens in Qinghai Province,posing a significant threat to both local animal husbandry and human health.It underscores the need to enhance systematic monitoring of tick-borne pathogens in the local population and livestock.
