(Molecular Plant 16,1832-1846,November 62023)The originally published version of this manuscript contained several small mistakes in the labels and legends of main and supplemental figures,along with a few small mista...(Molecular Plant 16,1832-1846,November 62023)The originally published version of this manuscript contained several small mistakes in the labels and legends of main and supplemental figures,along with a few small mistakes in the main text,that have now been corrected.Specifically,in Figure 6G,the label“NILGY31^(Pijx)”has been corrected to“NIL^(Pijx)”,and in the legend of Figure 6H,“PPL^(Pijx Piz-t)”and“PPL^(Pijx Pigm)”have been corrected to“NIL^(Piz-t Pijx)”and“PPL^(Pigm Pijx).”In Figure S8A。展开更多
Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t ...Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.展开更多
Phenolamides(PAs), a diverse group of specialized metabolites, including hydroxycinnamoylputrescine(HP), hydroxycinnamoylagmatine, and hydroxycinnamoyltryptamine, are important in plant resistance to biotic stress. Ho...Phenolamides(PAs), a diverse group of specialized metabolites, including hydroxycinnamoylputrescine(HP), hydroxycinnamoylagmatine, and hydroxycinnamoyltryptamine, are important in plant resistance to biotic stress. However, the genes involved in the biosynthesis and modulation of PAs have not been fully elucidated. This study identified an HP biosynthetic gene cluster in rice(Oryza sativa) comprising one gene(Os ODC) encoding a decarboxylase and two tandem-duplicated genes(Os PHT3 and Os PHT4)encoding putrescine hydroxycinnamoyl acyltransferases coexpressed in different tissues. Os ODC catalyzes the conversion of ornithine to putrescine, which is used in HP biosynthesis involving Os PHT3 and Os PHT4. Os PHT3 or Os PHT4 overexpression causes HP accumulation and cell death and putrescine hydroxycinnamoyl acyltransferases(PHT) activity-dependent resistance against the fungal pathogen Magnaporthe oryzae. Os ODC overexpression plants also confer enhanced resistance to M. oryzae.Notably, the basic leucine zipper transcription factor APIP5, a negative regulator of cell death, directly binds to the Os PHT4 promoter, repressing its transcription. Moreover, APIP5 suppression induces Os PHT4 expression and HP accumulation. Comparative genomic analysis revealed that the HP biosynthetic gene cluster is conserved in monocots. These results characterized a previously unidentified monocot-specific gene cluster that is involved in HP biosynthesis and contributes to defense and cell death in rice.展开更多
Protein degradation mediated by the ubiquitin-proteasome system(UPS)is involved in many cellular processes in animals and plants.In the UPS,E3 ligases(E3s)are essential for recognizing target proteins and promoting su...Protein degradation mediated by the ubiquitin-proteasome system(UPS)is involved in many cellular processes in animals and plants.In the UPS,E3 ligases(E3s)are essential for recognizing target proteins and promoting substrate degradation.One E3 ligase,SINA(seven in absentia),which was first identified in Drosophila,is involved in a variety of signaling transduction networks in both animals and plants.Here we discuss recent advances in understanding SINA?mediated regulation of plant growth and responses to abiotic and biotic stresses.展开更多
Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most important diseases of rice.Utilization of blast-resistance genes is the most economical,effective,and environmentally friendly way to cont...Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most important diseases of rice.Utilization of blast-resistance genes is the most economical,effective,and environmentally friendly way to control the disease.However,genetic resources with broad-spectrum resistance(BSR)that is effective throughout the rice growth period are rare.In this work,using a genome-wide association study,we identify a new blast-resistance gene,Pijx,which encodes a typical CC-NBS-LRR protein.Pijx is derived from a wild rice species and confers BSR to M.oryzae at both the seedling and panicle stages.The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments.Mechanistically,the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase β subunit(ATPb)via the 26S proteasome pathway.ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance,and interacts with OsRbohC to promote its degradation.Consistently,loss of ATPb function causes an increase in NAPDH content and ROS burst.Remarkably,when Pijx is introgressed into two japonica rice varieties,the introgression lines show BSR and increased yields that are approximately 51.59%and 79.31%higher compared with those of their parents in a natural blast disease nursery.In addition,we generate PPL^(Pijx Pigm) and PPL^(Pijx Piz-t) pyramided lines and these lines also have higher BSR to panicle blast compared with Pigm-or Piz-t-containing rice plants.Collectively,this study demonstrates that Pijx not only confers BSR to M.oryzae but also maintains high and stable rice yield,providing new genetic resources and molecular targets for breeding rice varieties with broad-spectrum blast resistance.展开更多
文摘(Molecular Plant 16,1832-1846,November 62023)The originally published version of this manuscript contained several small mistakes in the labels and legends of main and supplemental figures,along with a few small mistakes in the main text,that have now been corrected.Specifically,in Figure 6G,the label“NILGY31^(Pijx)”has been corrected to“NIL^(Pijx)”,and in the legend of Figure 6H,“PPL^(Pijx Piz-t)”and“PPL^(Pijx Pigm)”have been corrected to“NIL^(Piz-t Pijx)”and“PPL^(Pigm Pijx).”In Figure S8A。
基金This work was supported by grants from the National Natural Science Foundation of China(31822041,31901829,and 31972225)the National Key Research and Development Program of China(2016YFD0100600)the China Postdoctoral Science Foundation(2019M660894).
文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.
基金supported by grants from the National Natural Science Foundation of China (31822041 and 31972225)the National Key Research and Development Program of China (2016YFD0100600) to Yuese Ning+5 种基金the National Natural Science Foundation of China (U20A2021) to Ruyi Wangthe National Natural Science Foundation of China (31801692) to Fan Zhangthe State Key Program of National Natural Science Foundation of China (31530052)the National Science Fund for Distinguished Young Scholars (31625021)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (31821005)the Hainan University Start up Fund (KYQD(ZR)1866 to Jie Luo。
文摘Phenolamides(PAs), a diverse group of specialized metabolites, including hydroxycinnamoylputrescine(HP), hydroxycinnamoylagmatine, and hydroxycinnamoyltryptamine, are important in plant resistance to biotic stress. However, the genes involved in the biosynthesis and modulation of PAs have not been fully elucidated. This study identified an HP biosynthetic gene cluster in rice(Oryza sativa) comprising one gene(Os ODC) encoding a decarboxylase and two tandem-duplicated genes(Os PHT3 and Os PHT4)encoding putrescine hydroxycinnamoyl acyltransferases coexpressed in different tissues. Os ODC catalyzes the conversion of ornithine to putrescine, which is used in HP biosynthesis involving Os PHT3 and Os PHT4. Os PHT3 or Os PHT4 overexpression causes HP accumulation and cell death and putrescine hydroxycinnamoyl acyltransferases(PHT) activity-dependent resistance against the fungal pathogen Magnaporthe oryzae. Os ODC overexpression plants also confer enhanced resistance to M. oryzae.Notably, the basic leucine zipper transcription factor APIP5, a negative regulator of cell death, directly binds to the Os PHT4 promoter, repressing its transcription. Moreover, APIP5 suppression induces Os PHT4 expression and HP accumulation. Comparative genomic analysis revealed that the HP biosynthetic gene cluster is conserved in monocots. These results characterized a previously unidentified monocot-specific gene cluster that is involved in HP biosynthesis and contributes to defense and cell death in rice.
基金grants from the National Natural Science Foundation of China(31822041 and 31571944)to Y.N.the US National Science Foundation to G.L.W.(IOS#1120949).
文摘Protein degradation mediated by the ubiquitin-proteasome system(UPS)is involved in many cellular processes in animals and plants.In the UPS,E3 ligases(E3s)are essential for recognizing target proteins and promoting substrate degradation.One E3 ligase,SINA(seven in absentia),which was first identified in Drosophila,is involved in a variety of signaling transduction networks in both animals and plants.Here we discuss recent advances in understanding SINA?mediated regulation of plant growth and responses to abiotic and biotic stresses.
基金supported by the National Natural Science Foundation of China(grants No.31971868,32161143009,and 31971873)the earmarked fund for CARS-01-88CARS-01-88,the Core Provenance Project in Jiangsu Province(JBGS(2021)035)+2 种基金the Key R&D Program of Jiangsu Province Modern Agriculture(BE2021334,BE2022336)the Ministry of Science and Technology/State Administration of Foreign Experts Affairs(GL20200114001)the Jiangsu Province Natural Science Fund(BK20201218).
文摘Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most important diseases of rice.Utilization of blast-resistance genes is the most economical,effective,and environmentally friendly way to control the disease.However,genetic resources with broad-spectrum resistance(BSR)that is effective throughout the rice growth period are rare.In this work,using a genome-wide association study,we identify a new blast-resistance gene,Pijx,which encodes a typical CC-NBS-LRR protein.Pijx is derived from a wild rice species and confers BSR to M.oryzae at both the seedling and panicle stages.The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments.Mechanistically,the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase β subunit(ATPb)via the 26S proteasome pathway.ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance,and interacts with OsRbohC to promote its degradation.Consistently,loss of ATPb function causes an increase in NAPDH content and ROS burst.Remarkably,when Pijx is introgressed into two japonica rice varieties,the introgression lines show BSR and increased yields that are approximately 51.59%and 79.31%higher compared with those of their parents in a natural blast disease nursery.In addition,we generate PPL^(Pijx Pigm) and PPL^(Pijx Piz-t) pyramided lines and these lines also have higher BSR to panicle blast compared with Pigm-or Piz-t-containing rice plants.Collectively,this study demonstrates that Pijx not only confers BSR to M.oryzae but also maintains high and stable rice yield,providing new genetic resources and molecular targets for breeding rice varieties with broad-spectrum blast resistance.
