Objective Triple-negative breast cancer(TNBC)is the breast cancer subtype with the worst prognosis,and lacks effective therapeutic targets.Colony stimulating factors(CSFs)are cytokines that can regulate the production...Objective Triple-negative breast cancer(TNBC)is the breast cancer subtype with the worst prognosis,and lacks effective therapeutic targets.Colony stimulating factors(CSFs)are cytokines that can regulate the production of blood cells and stimulate the growth and development of immune cells,playing an important role in the malignant progression of TNBC.This article aims to construct a novel prognostic model based on the expression of colony stimulating factors-related genes(CRGs),and analyze the sensitivity of TNBC patients to immunotherapy and drug therapy.Methods We downloaded CRGs from public databases and screened for differentially expressed CRGs between normal and TNBC tissues in the TCGA-BRCA database.Through LASSO Cox regression analysis,we constructed a prognostic model and stratified TNBC patients into high-risk and low-risk groups based on the colony stimulating factors-related genes risk score(CRRS).We further analyzed the correlation between CRRS and patient prognosis,clinical features,tumor microenvironment(TME)in both high-risk and low-risk groups,and evaluated the relationship between CRRS and sensitivity to immunotherapy and drug therapy.Results We identified 842 differentially expressed CRGs in breast cancer tissues of TNBC patients and selected 13 CRGs for constructing the prognostic model.Kaplan-Meier survival curves,time-dependent receiver operating characteristic curves,and other analyses confirmed that TNBC patients with high CRRS had shorter overall survival,and the predictive ability of CRRS prognostic model was further validated using the GEO dataset.Nomogram combining clinical features confirmed that CRRS was an independent factor for the prognosis of TNBC patients.Moreover,patients in the high-risk group had lower levels of immune infiltration in the TME and were sensitive to chemotherapeutic drugs such as 5-fluorouracil,ipatasertib,and paclitaxel.Conclusion We have developed a CRRS-based prognostic model composed of 13 differentially expressed CRGs,which may serve as a useful tool for predicting the prognosis of TNBC patients and guiding clinical treatment.Moreover,the key genes within this model may represent potential molecular targets for future therapies of TNBC.展开更多
目的了解长沙市男男性行为人群(men who have sex with men,MSM)艾滋病知识知晓情况、性行为特征。方法基于长沙市青彩防艾志愿服务中心和中大阳光社工服务中心招募志愿者,采取滚雪球抽样法通过匿名填写问卷调查长沙市MSM人群,采用SPSS ...目的了解长沙市男男性行为人群(men who have sex with men,MSM)艾滋病知识知晓情况、性行为特征。方法基于长沙市青彩防艾志愿服务中心和中大阳光社工服务中心招募志愿者,采取滚雪球抽样法通过匿名填写问卷调查长沙市MSM人群,采用SPSS 19. 0软件进行统计分析。结果本次研究共调查150例MSM人群,艾滋病基本知识知晓率为86. 0%(129/150)。人口学特征方面,不同年龄、学历、收入人群艾滋病知识知晓率差异有统计学意义,年龄越大、学历越高、收入越高知晓率越高。性行为方面,首次发生插入性男男性交年龄<18岁的占32. 7%,≥18岁组艾滋病知识知晓率明显高于<18岁组(X^2=4. 315,P=0. 038),过去6个月的性伴侣数> 1个的占46. 7%,半年内仅偶尔或从未使用安全套的分别为29. 3%和6. 7%。结论年龄较小,学历较低和月收入较低的长沙市MSM人群艾滋病知识知晓率相对较低,首次性交年龄低龄化,应针对这些特征的MSM人群及时采取措施,进行高危性行为干预。展开更多
文摘Objective Triple-negative breast cancer(TNBC)is the breast cancer subtype with the worst prognosis,and lacks effective therapeutic targets.Colony stimulating factors(CSFs)are cytokines that can regulate the production of blood cells and stimulate the growth and development of immune cells,playing an important role in the malignant progression of TNBC.This article aims to construct a novel prognostic model based on the expression of colony stimulating factors-related genes(CRGs),and analyze the sensitivity of TNBC patients to immunotherapy and drug therapy.Methods We downloaded CRGs from public databases and screened for differentially expressed CRGs between normal and TNBC tissues in the TCGA-BRCA database.Through LASSO Cox regression analysis,we constructed a prognostic model and stratified TNBC patients into high-risk and low-risk groups based on the colony stimulating factors-related genes risk score(CRRS).We further analyzed the correlation between CRRS and patient prognosis,clinical features,tumor microenvironment(TME)in both high-risk and low-risk groups,and evaluated the relationship between CRRS and sensitivity to immunotherapy and drug therapy.Results We identified 842 differentially expressed CRGs in breast cancer tissues of TNBC patients and selected 13 CRGs for constructing the prognostic model.Kaplan-Meier survival curves,time-dependent receiver operating characteristic curves,and other analyses confirmed that TNBC patients with high CRRS had shorter overall survival,and the predictive ability of CRRS prognostic model was further validated using the GEO dataset.Nomogram combining clinical features confirmed that CRRS was an independent factor for the prognosis of TNBC patients.Moreover,patients in the high-risk group had lower levels of immune infiltration in the TME and were sensitive to chemotherapeutic drugs such as 5-fluorouracil,ipatasertib,and paclitaxel.Conclusion We have developed a CRRS-based prognostic model composed of 13 differentially expressed CRGs,which may serve as a useful tool for predicting the prognosis of TNBC patients and guiding clinical treatment.Moreover,the key genes within this model may represent potential molecular targets for future therapies of TNBC.
文摘目的了解长沙市男男性行为人群(men who have sex with men,MSM)艾滋病知识知晓情况、性行为特征。方法基于长沙市青彩防艾志愿服务中心和中大阳光社工服务中心招募志愿者,采取滚雪球抽样法通过匿名填写问卷调查长沙市MSM人群,采用SPSS 19. 0软件进行统计分析。结果本次研究共调查150例MSM人群,艾滋病基本知识知晓率为86. 0%(129/150)。人口学特征方面,不同年龄、学历、收入人群艾滋病知识知晓率差异有统计学意义,年龄越大、学历越高、收入越高知晓率越高。性行为方面,首次发生插入性男男性交年龄<18岁的占32. 7%,≥18岁组艾滋病知识知晓率明显高于<18岁组(X^2=4. 315,P=0. 038),过去6个月的性伴侣数> 1个的占46. 7%,半年内仅偶尔或从未使用安全套的分别为29. 3%和6. 7%。结论年龄较小,学历较低和月收入较低的长沙市MSM人群艾滋病知识知晓率相对较低,首次性交年龄低龄化,应针对这些特征的MSM人群及时采取措施,进行高危性行为干预。
