In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and ...In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.展开更多
本文以作者于2020年4月23日发表的"Appraisals in the Time of Pandemic"为基础(第一部分),并经与作者充分讨论,将作者于2014年11月25日发表的"How to Get an Accurate Desktop Appraisal"相关内容纳入本文(第二部...本文以作者于2020年4月23日发表的"Appraisals in the Time of Pandemic"为基础(第一部分),并经与作者充分讨论,将作者于2014年11月25日发表的"How to Get an Accurate Desktop Appraisal"相关内容纳入本文(第二部分)。本文反映了国外同行对于疫情等特殊情况下在不进行现场勘查程序下如何进行评估的作法,供国内同仁参考。同时此文提醒关注不进行现场勘查程序并不意味着低成本或更简单,相反评估师需要与客户进行更多的沟通与协调,需要客户提供更多详细的资料。作者为资深设备评估师,故文中观点多是从设备评估的角度,提请关注。展开更多
文摘In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.
文摘本文以作者于2020年4月23日发表的"Appraisals in the Time of Pandemic"为基础(第一部分),并经与作者充分讨论,将作者于2014年11月25日发表的"How to Get an Accurate Desktop Appraisal"相关内容纳入本文(第二部分)。本文反映了国外同行对于疫情等特殊情况下在不进行现场勘查程序下如何进行评估的作法,供国内同仁参考。同时此文提醒关注不进行现场勘查程序并不意味着低成本或更简单,相反评估师需要与客户进行更多的沟通与协调,需要客户提供更多详细的资料。作者为资深设备评估师,故文中观点多是从设备评估的角度,提请关注。
