Unlike annual flowering plants that exhibit a single reproductive cycle encompassing vegetative growth,flowering,and senescence within a single year,perennial woody trees undergo a transition between vegetative and re...Unlike annual flowering plants that exhibit a single reproductive cycle encompassing vegetative growth,flowering,and senescence within a single year,perennial woody trees undergo a transition between vegetative and reproductive growth every year after a long juvenile phase.Most woody fruit trees bloom when it turns warm in spring,and the fruit ripens in summer or autumn.However,as a member of the Maloideae subfamily,the loquat tree blooms in the cold autumn and winter,and the fruit matures in the spring.To explore the regulatory mechanism of loquat flowering,we characterized two FLOWERING LOCUS T homologous genes in loquat,EjFT1 and EjFT2.qRT-PCR results revealed that EjFT1 and EjFT2 exhibited nearly opposite expression patterns in various tissues.EjFT1 was mainly expressed in young tissues;EjFT2 was mainly expressed in mature leaves,open flowers,and fruits.EjFT2 exhibited an obvious circadian rhythm and was regulated by EjCO.After the trees were exposed to short-day conditions or sprayed with exogenous GA_(3),the expression of EjFT2 was strongly inhibited,and the loquat tree did not produce floral buds.Furthermore,the yeast two-hybrid,bimolecular fluorescence complementation,and Dualluciferase assay experiments revealed that both EjFT1 and EjFT2 interacted with EjFD,with the EjFT2-EjFD protein complex enhancing the activity of EjAP1-1 and EjAP1-2 promoters,while EjFT1-EjFD inhibited the activity of the EjAP1-1 promoter.Protein structural analysis of EjFT1and EjFT2 suggested that differences in amino acid residues at Val_(123)/Leu_(123),Ser_(157)/Ala_(157),and Val_(158)/Ala_(158)may be the reason for their functional differences.Our results showed that EjFT1 and EjFT2 may cooperatively regulate the floral bud differentiation of loquat by competitively binding with EjFD.EjFT2 regulates the onset of loquat floral bud differentiation by responding to photoperiod and gibberellin signals,while EjFT1 is involved in the vegetative growth of loquat.These findings provide important clues for the investigation of the regulatory mechanism of loquat flowering and advance the exploration into the multiple roles of FT homologous genes in regulating the reproductive transformation and vegetative growth of flowering plants.展开更多
Objective:To explore the role of specialized group management in the quality control of perioperative nursing.Methods:45 surgical nurses from our hospital were selected as the research subjects.Traditional operating r...Objective:To explore the role of specialized group management in the quality control of perioperative nursing.Methods:45 surgical nurses from our hospital were selected as the research subjects.Traditional operating room management was adopted from July 2019 to June 2020,and specialized group management was adopted from July 2020 to June 2021.The surgeon’s satisfaction,surgical nurses’core professional competence,and surgical patients’satisfaction were obtained through surveys and the results were analyzed.Results:Surgeon satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Besides,surgical nurses’core professional competency scores before the implementation of specialized group management were significantly lower than after its implementation(P<0.05).Lastly,surgical patients’satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Conclusion:Specialized group management helps to improve the quality of perioperative care and should be applied in clinical practice.展开更多
Molybdenum carbide(Mo_(2)C)is a promising non-noble metal electrocatalyst with electronic structures similar to Pt for hydrogen evolution reaction(HER).However,strong H^(*)adsorption at the Mo sites hinders the improv...Molybdenum carbide(Mo_(2)C)is a promising non-noble metal electrocatalyst with electronic structures similar to Pt for hydrogen evolution reaction(HER).However,strong H^(*)adsorption at the Mo sites hinders the improvement of HER performance.Here,we synthesized monodisperse hollow Mo_(2)C nanoreactors,in which the carbon dots(CD)were in situ formed onto the surface of Mo_(2)C through carburization reactions.According to finite element simulation and analysis,the CD@Mo_(2)C possesses better mesoscale diffusion properties than Mo_(2)C alone.The optimized CD@Mo_(2)C nanoreactor demonstrates superior HER performance in alkaline electrolyte with a low overpotential of 57 mV at 10 mA cm^(−2),which is better than most Mo_(2)C-based electrocatalysts.Moreover,CD@Mo_(2)C exhibits excellent electrochemical stability during 240 h,confirmed by operando Raman and X-ray diffraction(XRD).Density functional theory(DFT)calculations show that carbon dots cause the d-band center of CD@Mo_(2)C to shift away from Fermi level,promoting water dissociation and the desorption of H^(*).This study provides a reasonable strategy towards high-activity Mo-based HER eletrocatalysts by modulating the strength of Mo–H bonds.展开更多
The effect of scanning strategy on the microstructure and properties of GH3536 Ni-based superalloy prepared by Laser Powder Bed Fusion was investigated,for the purpose of building high quality hydrocyclone part.The re...The effect of scanning strategy on the microstructure and properties of GH3536 Ni-based superalloy prepared by Laser Powder Bed Fusion was investigated,for the purpose of building high quality hydrocyclone part.The results show that the strength of Z67°(a zone with 67°hatch angle strategy)specimen is the highest among the four scanning strategies(0°,67°,90°and Z67°),with yield strength and tensile strength of 681 MPa and 837 MPa,respectively.Selective orientation of crystals occurs during the forming process because the longitudinal section of the specimen exhibits a high texture strength in(001).As the stretching proceeds,the plastic deformation mechanism of the specimen gradually changes from slip to twin-dominated,a substantial amount of twinning is observed in the region where the deformation of the specimen reaches 80%.The additive manufacturing simulation suite:Ansys Additive is used to simulate the stress and deformation of the part during the process,and the displacement results are consistent with the experimental phenomena.According to the simulation results,the structure design is optimized and the surface quality of the part is improved.The results show that the support of the part is more reasonable when the overhang angle is 45°.展开更多
[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infect...[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infected with nematodes were irrigated with a Chinese herbal medicine regulator at two different concentrations,and the control effect on leaf spot nematode disease of the plant was studied by measuring the number of nematodes,the contents of chlorophyll,malondialdehyde,soluble sugar and free proline in the leaves of the plants in the soil.[Results]Compared with the control,the two concentrations of Chinese herbal medicine regulator significantly reduced the number of nematodes in the soil,increased the contents of chlorophyll(mainly chlorophyll a),soluble sugar and free proline in the leaves of C.spicatus,and decreased the content of malondialdehyde.It indicated that the Chinese herbal medicine regulator could effectively control the spread of nematodes and had certain effect on the recovery of C.spicatus plants.[Conclusions]The use of Chinese herbal medicine regulators is helpful to curb the occurrence of leaf spot nematode disease in C.spicatus by biological prevention and control means,and provides theoretical guidance for the development of the green industry of C.spicatus.展开更多
Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its production.To explore the artificial regulation of loquat flowering time,we remove...Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its production.To explore the artificial regulation of loquat flowering time,we removed the main inflorescence(by cutting it)to induce reflowering.For different loquat tree cultivars with different stages,the inflorescence was removed by cutting the main floral axis at two alternative positions:the upper or the lower position beneath the inflorescence,and it was found that the proportion of reflowering resulting from removing the upper position of the main floral axis of yellow-flesh loquat cultivars during the full-bloom stage was the highest.In addition,compared with those of the normal-growing panicles,the number of flower buds and branch axes of the reflowering panicles decreased significantly after cutting.Importantly,these newly produced inflorescences flowered 2–4 months later than normal-growing inflorescences did,effectively prolonging both the flowering and fruiting time.In addition,qRT-PCR results showed that EjFT1,EjFT2,EjAP1–1 and EjAP1–2 were highly expressed in the floral axis.These findings highlighted a new method for extending the production cycle of loquat and provided a reference for the flowering regulation of loquat and other economically important fruit tree species.展开更多
A 2D axial symmetry fluid model is applied to study the features of an atmospheric-pressure argon(Ar) plasma jet propagating into ambient nitrogen(N_(2)) driven by a pulsed voltage,emphasizing the influence of gas vel...A 2D axial symmetry fluid model is applied to study the features of an atmospheric-pressure argon(Ar) plasma jet propagating into ambient nitrogen(N_(2)) driven by a pulsed voltage,emphasizing the influence of gas velocity on the dynamic characteristics of the jet. The results show that the Ar jet exhibits a cylindrical-shaped channel and the jet channel gradually shrinks with the increase in propagation length. The jet propagation velocity varies with time. Inside the dielectric tube, the plasma jet accelerates propagation and reaches its maximum value near the nozzle. Exiting the tube, its velocity quickly decreases and when approaching the metal plane,the decrease in jet velocity slows down. The increase in gas speed results in the variation of jet spatial distribution. The electron density presents a solid structure at lower gas flow speeds,whereas an annular structure can be observed under the higher gas flow velocity in the ionization head. The jet length increases with the flow velocity. However, when the flow velocity exceeds a critical value, the increase in the rate of the plasma jet length slows down. In addition, the gas velocity effect on the generation and transport of the reactive particles is also studied and discussed.展开更多
Hydrogen peroxide (H2O2), first synthesized in 1818 through the acidification of barium peroxide (BaO2) with nitric acid, is a clear and colorless liquid which is entirely miscible with water and variety of organic so...Hydrogen peroxide (H2O2), first synthesized in 1818 through the acidification of barium peroxide (BaO2) with nitric acid, is a clear and colorless liquid which is entirely miscible with water and variety of organic solvents such as carboxylic acid and esters. Anthraquinone process (an old production process of H2O2), a batch process carried out in large facilities is an energy demanding process that requires large facilities, and involves oxidation of anthraquinone molecules and sequential hydrogenation. Moreover, the direct synthesis method enables production in a continuous mode as well as it permits small scale, decentralized production. Many drawbacks associated with these processes such as, energetic inefficiency and inherent disadvantages have motivated researchers, industry and academia to find out alternative for synthesis of H2O2. Electrochemical route based on catalyst selectively reduce oxygen to hydrogen peroxide. O2 is cathodically reduced to produce H2O2 via 2-electron pathway or 4-electron pathway to get H2O. Electrolysis of water has an important place in storage and electrochemical energy conversion process where problem is to choose a sufficiently stable and active electrode for anodic oxygen evolution reaction. Most commonly used catalysts on the cathode are carbon based materials such as carbon black, carbon nanotubes, graphite, carbon sponge, and carbon fiber. In perspective of expanding demand of production and usage of hydrogen peroxide we review the past literature to summarize different production processes of H2O2. In this review, we mainly focus on electrochemical production of hydrogen peroxide along with other alternatives, such as anthraquinone method for industrial H2O2 production and direct synthesis process. We also review the catalytic activity, selectivity and stability for enhanced yield of H2O2. From revision of last two decade’s literature including experimental and theoretical data;we argue that successful implementation of electrochemical H2O2 production can be realized on the basis of stable, active and selective catalyst.展开更多
Solid acid catalyst plays a crucial role in the petroleum refinery industry and bio-refinery technology.In this work,p-phenolsulfonic acid(PSA)was successfully grafted onto the surface of KH560-modified zirconium phos...Solid acid catalyst plays a crucial role in the petroleum refinery industry and bio-refinery technology.In this work,p-phenolsulfonic acid(PSA)was successfully grafted onto the surface of KH560-modified zirconium phosphate(K-ZrP)in a facile routine.The structure and property of this organic-inorganic combined solid acid PSA/K-ZrP-x were characterized via XRD,FTIR,^(13)C solid-state NMR,TG,N_(2) adsorption-desorption,SEM,pyridine-adsorption FTIR and XPS technologies.The characterization results showed that KH560 can bond with ZrP and promote the grafting of PSA on the surface of K-ZrP via the condensation reaction between its epoxy ring and the phenolic hydroxyl group in PSA.Consequently,PSA/K-ZrP-2 exhibited excellent performance and stability in the transesterification between glycerol and methyl acetate among the tested H_(3)PW_(12)O_(40),Amber-lyst-45,HBEA,HZSM-5,ZrP,AlCl_(3) and FeCl_(3) catalysts.The calculated conversion of glycerol reached 81.3%with a 97.9%selectivity for monoacetin(MAG)and diacetin(DAG)with a 2.2%dosage of[H^(+)]at 100℃ for 4 h.The highest specific activity of PSA/K-ZrP-2 reached 24028.2 mg-glycerol/g-cat/h in a short reaction time(at 0.17 h),and it could be recycled five times without obvious deactivation.展开更多
Objective:to explore the changes of the F-wave in the posterior tibial nerve of rabbits after different levels of lumbar spinal cord ischaemic injury and its correlation with motor function and the extent of lumbar sp...Objective:to explore the changes of the F-wave in the posterior tibial nerve of rabbits after different levels of lumbar spinal cord ischaemic injury and its correlation with motor function and the extent of lumbar spinal cord pathological damage.Methods:thirty New Zealand rabbits were randomly divided into 6 groups.The control group(n=5)was used to exclude the influence of anaesthesia and surgery on the F-wave.Different levels of lumbar arteries were ligated in the five experimental groups(n=5).The F-wave was recorded to observe the changes in the acute phase of spinal cord ischaemia.The correlation between the changes of the F-wave in the acute reversible phase and the motor function of the spinal cord was analysed.Motor functions were assessed after surgery and 2 d after vascular ligation.The specimens were taken 2 d after ligation for histopathologic observation.Results:the results for the control group indicated that anaesthesia and surgery did not affect the F-wave results.There was no statistically significant difference in the F-wave amplitudes and latency before and after ligation in the 1 and 2 level ligation groups.The F-wave changed immediately after ligation in the 3,4 and 5 ligation groups.The latency of the F-wave gradually extended,the amplitude of the F-wave gradually reduced.The amplitude variations of the F-wave were positively correlated with the motor function 2 d after ligation,there was a statistically significant difference.With the increase in the number of vascular ligation,the degree of destruction of the motor neurons in the anterior horn of the spinal cord in the pathological specimens increased.Conclusion:the F-waves in the posterior tibial nerve of rabbits were found to be sensitive to the lumbar spinal cord ischaemic injury and specific to predict motor function.展开更多
To the Editor:Allergic bronchopulmonary aspergillosis(ABPA)is an allergic lung disease caused by hypersensitivity to antigens from Aspergillus,and it most commonly occurs in patients with asthma or cysticbrosis.[1]The...To the Editor:Allergic bronchopulmonary aspergillosis(ABPA)is an allergic lung disease caused by hypersensitivity to antigens from Aspergillus,and it most commonly occurs in patients with asthma or cysticbrosis.[1]The main clinical characteristics of the disease are those related to elevated serum immunoglubulin(Ig)E level,positive Aspergillus fumigatus(A.fumigatus)specic IgE/IgG,repeated pulmonary inltration,and bronchiectasis.However,due to the lack of unied diagnostic criteria and screening methods,ABPA is always underdiagnosed.In 2021,new diagnostic criteria were proposed for allergic bronchopulmonary mycosis(ABPM)in the Japanese population.展开更多
Lubrication failure accompanying with blackening phenomenon significantly reduces the long-running operational reliability of porous polymide(PPI)lubricated with poly-α-olefin(PAO)oil.Here,the effects of lubrication ...Lubrication failure accompanying with blackening phenomenon significantly reduces the long-running operational reliability of porous polymide(PPI)lubricated with poly-α-olefin(PAO)oil.Here,the effects of lubrication condition and counter-surface chemistry on the blackening failure of PAO impregnated PPI were studied through the comparison of the tribological tests against GCr15 steel ball and Al_(2)O_(3)ceramic ball with and without PAO oil lubrication.Black products were found to be formed on the PAO impregnated PPI surface slid against steel ball or Al_(2)O_(3)ball added with iron nano-particles,but be absent under the conditions without iron or PAO oil.Further analysis indicated that the iron-catalyzed splitting of PAO oil into small molecule alkanes and following the formation of black organic matter should be mainly responsible for the blackening phenomenon.Molecular dynamic(MD)simulations demonstrated that the iron facilitated the separation of hydrogen atom and the following broken of C–C bonds in PAO molecules,final resulting in the splitting of PAO oil.展开更多
Cas12a(Cpf1),a Class 2 Type V CRISPR/Cas nuclease,has several unique attributes for genome editing and may provide a valuable alternative to Cas9.However,a low editing efficiency due to temperature sensitivity and ins...Cas12a(Cpf1),a Class 2 Type V CRISPR/Cas nuclease,has several unique attributes for genome editing and may provide a valuable alternative to Cas9.However,a low editing efficiency due to temperature sensitivity and insufficient cleavage activity of the Cas12a nuclease are major obstacles to its broad application.In this report,we generated two variants,ttAsCas12 Ultra and ttLbCas12a Ultra harboring three(E174R,M537R,and F870L)or two(D156R and E795L)mutations,respectively,by combining the mutations from the temperature-tolerant variants ttAsCas12a(E174R)and ttLbCas12a(D156R),and those from the highly active variants AsCas12a Ultra(M537R and F870L)and LbCas12a Ultra(E795L).We compared editing efficiencies of the five resulting Cas12a variants(LbCas12a,ttLbCas12a,ttLbCas12a Ultra,AsCas12a Ultra,and ttAsCas12 Ultra)at six target sites of four genes in Arabidopsis(Arabidopsis thaliana).The variant ttLbCas12a Ultra,harboring the D156R and E795L mutations,exhibited the highest editing efficiency of all variants tested in Arabidopsis and can be used to generate homozygous or biallelic mutants in a single generation in Arabidopsis plants grown at 22 C.In addition,optimization of ttLbCas12a Ultra,by varying nuclear localization signal sequences and codon usage,further greatly improved editing efficiency.Collectively,our results indicate that ttLbCas12a Ultra is a valuable alternative to Cas9 for editing genes or promoters in Arabidopsis.展开更多
Fuels and oleochemicals have been chemically produced fromabundant biological oils or fatty acids for more than a century,and modern biotechnology is now accelerating the advances infatty acid chemistry [1,2]. A numbe...Fuels and oleochemicals have been chemically produced fromabundant biological oils or fatty acids for more than a century,and modern biotechnology is now accelerating the advances infatty acid chemistry [1,2]. A number of enzymes responsible forthe biosynthesis of hydrocarbons from fatty acids have been discovered, providing a promising strategy for enzymatic synthesisof fatty hydrocarbons [3]. CYP152 peroxygenases have attracted agreat deal of attention due to their ability to one-step oxidativelydecarboxylate or hydroxylate fatty acids using H2O2 as sole oxidant, generating valuable a-olefins or hydroxylated fatty acids[4,5] (Fig. 1a). In addition to a-olefins as biofuels and chemicalintermediates, hydroxylated fatty acids are also an important classof compounds with various applications in food, cosmetic, pharmaceutical, and biomaterial industries [6,7] (Fig. 1b). Among them,P450BSb is considered as the most promising CYP152 peroxygenasefor practical applications due to its high stability and activity tocatalyze both b-hydroxylation (the preferred reaction) and decarboxylation (the side reaction) of fatty acids that are both mechanistically initiated by Cb-H abstraction. Of note, directed evolutioncould remodel its catalytic preference on decarboxylation orb-hydroxylation (e.g., the P450BSb-DC mutant with 67% decarboxylation selectivity) [5].展开更多
Concerns regarding human health and food safety have generated interest in developing simple,accurate,and cost-effective strategies for evaluating organophosphorus pesticide(OP)residues.In this study,nitrogen-doped ca...Concerns regarding human health and food safety have generated interest in developing simple,accurate,and cost-effective strategies for evaluating organophosphorus pesticide(OP)residues.In this study,nitrogen-doped carbon dots(N-CDs)were synthesized from a common low-cost precursor via a simple pyrolysis process.The fluorescence of the N-CDs can be suppressed by p-nitrophenol,which is the hydrolysis product of the p-nitrophenyl phosphate salt catalyzed by alkaline phosphatase(ALP).A fluorescent turn-on assay for the inhibitory effect of glyphosate was developed with a low detection limit and wide linear range.Moreover,the feasibility of visualizing OPs in vegetables was demonstrated by the fluorescence imaging of glyphosate on cabbage leaves.This research not only offers a facile method for the synthesis of highly fluorescent CDs but also inspires the development of effective multi-mode sensing platforms that include fluorescent testing and imaging for monitoring pesticide residues.展开更多
In order to further promote the chestnut industrial chain,a large number of chestnut byproducts(chestnut leaves,chestnut shells,chestnut flowers,etc.)are produced every year.These chestnut byproducts will be comprehen...In order to further promote the chestnut industrial chain,a large number of chestnut byproducts(chestnut leaves,chestnut shells,chestnut flowers,etc.)are produced every year.These chestnut byproducts will be comprehensively developed and utilized in different degrees,such as in-depth research and development in the fields of animal husbandry,agriculture,and cosmetics,so as to achieve the effects of accelerating the high-quality development of chestnut industry,realizing the green cycle of resources,reducing waste and promoting the development of rural industries.展开更多
Regulating the interfacial charge transfer is pivotal for elucidating the kinetics of engineering the interface between the light-harvesting semiconductor and the substrate/catalyst for photoelectrocatalytic water spl...Regulating the interfacial charge transfer is pivotal for elucidating the kinetics of engineering the interface between the light-harvesting semiconductor and the substrate/catalyst for photoelectrocatalytic water splitting.In this study,we constructed a superior Ti-doped hematite photoanode(TiFeO)by employing SnOx as an electron transfer mediator,partially oxidized graphene(pGO)as a hole transfer mediator,and molecular Co cubane as a water oxidation catalyst.The Co/pGO/TiFeO/SnO_(x)integrated system achieves a photocurrent density of 2.52 mA cm–2 at 1.23 VRHE,which is 2.4 times higher than bare photoanode(1.04 mA cm^(-2)),with operational stability up to 100 h.Kinetic measurements indicate that pGO can promote charge transfer from TiFeO to the Co cubane catalyst.In contrast,SnOx reduces charge recombination at the interface between TiFeO and the fluorinated tin oxide substrate.In-situ infrared spectroscopy shows the formation of an O–O bonded intermediate during water oxidation.This study highlights the crucial role of incorporating dual charge-transfer mediators into photoelectrodes for efficient solar energy conversion.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515013095,2021A1515011048)the Open Fund of the Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region(Grant No.SY-KFFMR2022009Z)Shaoguan City Science and Technology Planning Project(Social Development Direction-Supporting Scientific Research Workers Project)(Grant No.200811094530739)。
文摘Unlike annual flowering plants that exhibit a single reproductive cycle encompassing vegetative growth,flowering,and senescence within a single year,perennial woody trees undergo a transition between vegetative and reproductive growth every year after a long juvenile phase.Most woody fruit trees bloom when it turns warm in spring,and the fruit ripens in summer or autumn.However,as a member of the Maloideae subfamily,the loquat tree blooms in the cold autumn and winter,and the fruit matures in the spring.To explore the regulatory mechanism of loquat flowering,we characterized two FLOWERING LOCUS T homologous genes in loquat,EjFT1 and EjFT2.qRT-PCR results revealed that EjFT1 and EjFT2 exhibited nearly opposite expression patterns in various tissues.EjFT1 was mainly expressed in young tissues;EjFT2 was mainly expressed in mature leaves,open flowers,and fruits.EjFT2 exhibited an obvious circadian rhythm and was regulated by EjCO.After the trees were exposed to short-day conditions or sprayed with exogenous GA_(3),the expression of EjFT2 was strongly inhibited,and the loquat tree did not produce floral buds.Furthermore,the yeast two-hybrid,bimolecular fluorescence complementation,and Dualluciferase assay experiments revealed that both EjFT1 and EjFT2 interacted with EjFD,with the EjFT2-EjFD protein complex enhancing the activity of EjAP1-1 and EjAP1-2 promoters,while EjFT1-EjFD inhibited the activity of the EjAP1-1 promoter.Protein structural analysis of EjFT1and EjFT2 suggested that differences in amino acid residues at Val_(123)/Leu_(123),Ser_(157)/Ala_(157),and Val_(158)/Ala_(158)may be the reason for their functional differences.Our results showed that EjFT1 and EjFT2 may cooperatively regulate the floral bud differentiation of loquat by competitively binding with EjFD.EjFT2 regulates the onset of loquat floral bud differentiation by responding to photoperiod and gibberellin signals,while EjFT1 is involved in the vegetative growth of loquat.These findings provide important clues for the investigation of the regulatory mechanism of loquat flowering and advance the exploration into the multiple roles of FT homologous genes in regulating the reproductive transformation and vegetative growth of flowering plants.
基金Hebei University Affiliated Hospital Youth Fund Scientific Research Project Project Number:2019Q017。
文摘Objective:To explore the role of specialized group management in the quality control of perioperative nursing.Methods:45 surgical nurses from our hospital were selected as the research subjects.Traditional operating room management was adopted from July 2019 to June 2020,and specialized group management was adopted from July 2020 to June 2021.The surgeon’s satisfaction,surgical nurses’core professional competence,and surgical patients’satisfaction were obtained through surveys and the results were analyzed.Results:Surgeon satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Besides,surgical nurses’core professional competency scores before the implementation of specialized group management were significantly lower than after its implementation(P<0.05).Lastly,surgical patients’satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Conclusion:Specialized group management helps to improve the quality of perioperative care and should be applied in clinical practice.
基金financially supported by the National Natural Science Foundation of China (22372001)Starting Fund for Scientific Research of High-Level Talents, Anhui Agricultural University (rc382108)+1 种基金Anhui Provincial Key Research and Development Plan (2022e07020037)Innovation and Entrepreneurship Training Program for College Students (X202310364204, S202210364046, X202310364209)
文摘Molybdenum carbide(Mo_(2)C)is a promising non-noble metal electrocatalyst with electronic structures similar to Pt for hydrogen evolution reaction(HER).However,strong H^(*)adsorption at the Mo sites hinders the improvement of HER performance.Here,we synthesized monodisperse hollow Mo_(2)C nanoreactors,in which the carbon dots(CD)were in situ formed onto the surface of Mo_(2)C through carburization reactions.According to finite element simulation and analysis,the CD@Mo_(2)C possesses better mesoscale diffusion properties than Mo_(2)C alone.The optimized CD@Mo_(2)C nanoreactor demonstrates superior HER performance in alkaline electrolyte with a low overpotential of 57 mV at 10 mA cm^(−2),which is better than most Mo_(2)C-based electrocatalysts.Moreover,CD@Mo_(2)C exhibits excellent electrochemical stability during 240 h,confirmed by operando Raman and X-ray diffraction(XRD).Density functional theory(DFT)calculations show that carbon dots cause the d-band center of CD@Mo_(2)C to shift away from Fermi level,promoting water dissociation and the desorption of H^(*).This study provides a reasonable strategy towards high-activity Mo-based HER eletrocatalysts by modulating the strength of Mo–H bonds.
基金the financial support of Technology Innovation Leadership Program for High-tech Industries(2020GK2031)Hunan Innovation Platform and Talent Plan(2022RC3033)Natural Science Foundation of Shandong Province(ZR2020ZD04).
文摘The effect of scanning strategy on the microstructure and properties of GH3536 Ni-based superalloy prepared by Laser Powder Bed Fusion was investigated,for the purpose of building high quality hydrocyclone part.The results show that the strength of Z67°(a zone with 67°hatch angle strategy)specimen is the highest among the four scanning strategies(0°,67°,90°and Z67°),with yield strength and tensile strength of 681 MPa and 837 MPa,respectively.Selective orientation of crystals occurs during the forming process because the longitudinal section of the specimen exhibits a high texture strength in(001).As the stretching proceeds,the plastic deformation mechanism of the specimen gradually changes from slip to twin-dominated,a substantial amount of twinning is observed in the region where the deformation of the specimen reaches 80%.The additive manufacturing simulation suite:Ansys Additive is used to simulate the stress and deformation of the part during the process,and the displacement results are consistent with the experimental phenomena.According to the simulation results,the structure design is optimized and the surface quality of the part is improved.The results show that the support of the part is more reasonable when the overhang angle is 45°.
基金Supported by Huangshan Science and Technology Planning Project(2022KN-02)School-level Key Project of Huangshan University(2022XKJZD004)+2 种基金First-class Discipline in Huangshan University(YLXK202101)Key Project of Natural Science Foundation of Department of Education of Anhui Province(2022AH051953)Innovation Training Program(PX-135245491).
文摘[Objectives]This study was conducted to investigate the control of Chinese herbal medicine regulators on leaf spot nematode disease,the main pest of Chloranthus spicatus(Thunb.)Makino.[Methods]C.spicatus plants infected with nematodes were irrigated with a Chinese herbal medicine regulator at two different concentrations,and the control effect on leaf spot nematode disease of the plant was studied by measuring the number of nematodes,the contents of chlorophyll,malondialdehyde,soluble sugar and free proline in the leaves of the plants in the soil.[Results]Compared with the control,the two concentrations of Chinese herbal medicine regulator significantly reduced the number of nematodes in the soil,increased the contents of chlorophyll(mainly chlorophyll a),soluble sugar and free proline in the leaves of C.spicatus,and decreased the content of malondialdehyde.It indicated that the Chinese herbal medicine regulator could effectively control the spread of nematodes and had certain effect on the recovery of C.spicatus plants.[Conclusions]The use of Chinese herbal medicine regulators is helpful to curb the occurrence of leaf spot nematode disease in C.spicatus by biological prevention and control means,and provides theoretical guidance for the development of the green industry of C.spicatus.
基金supported by Shaoguan City Science and Technology Planning Project(Social Development Direction-Supporting Scientific Research Workers Project)(Grant No.200811094530739)the Key Realm R&D Program of Guang Dong Provide(Grant No.2018B020202011)+2 种基金Open Fund of Key laboratory of Loquat Germplasm Innovation and Utilization(Putian University)Fujian Province University(Grant No.2019005)Guangdong Province College Students’Innovative and Entrepreneurial Training Program(Grant No.S202010576014X)。
文摘Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its production.To explore the artificial regulation of loquat flowering time,we removed the main inflorescence(by cutting it)to induce reflowering.For different loquat tree cultivars with different stages,the inflorescence was removed by cutting the main floral axis at two alternative positions:the upper or the lower position beneath the inflorescence,and it was found that the proportion of reflowering resulting from removing the upper position of the main floral axis of yellow-flesh loquat cultivars during the full-bloom stage was the highest.In addition,compared with those of the normal-growing panicles,the number of flower buds and branch axes of the reflowering panicles decreased significantly after cutting.Importantly,these newly produced inflorescences flowered 2–4 months later than normal-growing inflorescences did,effectively prolonging both the flowering and fruiting time.In addition,qRT-PCR results showed that EjFT1,EjFT2,EjAP1–1 and EjAP1–2 were highly expressed in the floral axis.These findings highlighted a new method for extending the production cycle of loquat and provided a reference for the flowering regulation of loquat and other economically important fruit tree species.
基金supported by National Natural Science Foundation of China(Nos.11775043,11675095 and 11505020)the Fundamental Research Funds for the Central Universities(No.DUT18LK31)。
文摘A 2D axial symmetry fluid model is applied to study the features of an atmospheric-pressure argon(Ar) plasma jet propagating into ambient nitrogen(N_(2)) driven by a pulsed voltage,emphasizing the influence of gas velocity on the dynamic characteristics of the jet. The results show that the Ar jet exhibits a cylindrical-shaped channel and the jet channel gradually shrinks with the increase in propagation length. The jet propagation velocity varies with time. Inside the dielectric tube, the plasma jet accelerates propagation and reaches its maximum value near the nozzle. Exiting the tube, its velocity quickly decreases and when approaching the metal plane,the decrease in jet velocity slows down. The increase in gas speed results in the variation of jet spatial distribution. The electron density presents a solid structure at lower gas flow speeds,whereas an annular structure can be observed under the higher gas flow velocity in the ionization head. The jet length increases with the flow velocity. However, when the flow velocity exceeds a critical value, the increase in the rate of the plasma jet length slows down. In addition, the gas velocity effect on the generation and transport of the reactive particles is also studied and discussed.
基金supported by the National Natural Science Foundation of China(21705056)the Young Taishan Scholars Program(tsqn201812080)+1 种基金the Natural Science Foundation of Shandong Province(ZR2019YQ10,ZR2017MB022,ZR2018BB057,ZR2018PB009)the Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201901).
文摘Hydrogen peroxide (H2O2), first synthesized in 1818 through the acidification of barium peroxide (BaO2) with nitric acid, is a clear and colorless liquid which is entirely miscible with water and variety of organic solvents such as carboxylic acid and esters. Anthraquinone process (an old production process of H2O2), a batch process carried out in large facilities is an energy demanding process that requires large facilities, and involves oxidation of anthraquinone molecules and sequential hydrogenation. Moreover, the direct synthesis method enables production in a continuous mode as well as it permits small scale, decentralized production. Many drawbacks associated with these processes such as, energetic inefficiency and inherent disadvantages have motivated researchers, industry and academia to find out alternative for synthesis of H2O2. Electrochemical route based on catalyst selectively reduce oxygen to hydrogen peroxide. O2 is cathodically reduced to produce H2O2 via 2-electron pathway or 4-electron pathway to get H2O. Electrolysis of water has an important place in storage and electrochemical energy conversion process where problem is to choose a sufficiently stable and active electrode for anodic oxygen evolution reaction. Most commonly used catalysts on the cathode are carbon based materials such as carbon black, carbon nanotubes, graphite, carbon sponge, and carbon fiber. In perspective of expanding demand of production and usage of hydrogen peroxide we review the past literature to summarize different production processes of H2O2. In this review, we mainly focus on electrochemical production of hydrogen peroxide along with other alternatives, such as anthraquinone method for industrial H2O2 production and direct synthesis process. We also review the catalytic activity, selectivity and stability for enhanced yield of H2O2. From revision of last two decade’s literature including experimental and theoretical data;we argue that successful implementation of electrochemical H2O2 production can be realized on the basis of stable, active and selective catalyst.
文摘Solid acid catalyst plays a crucial role in the petroleum refinery industry and bio-refinery technology.In this work,p-phenolsulfonic acid(PSA)was successfully grafted onto the surface of KH560-modified zirconium phosphate(K-ZrP)in a facile routine.The structure and property of this organic-inorganic combined solid acid PSA/K-ZrP-x were characterized via XRD,FTIR,^(13)C solid-state NMR,TG,N_(2) adsorption-desorption,SEM,pyridine-adsorption FTIR and XPS technologies.The characterization results showed that KH560 can bond with ZrP and promote the grafting of PSA on the surface of K-ZrP via the condensation reaction between its epoxy ring and the phenolic hydroxyl group in PSA.Consequently,PSA/K-ZrP-2 exhibited excellent performance and stability in the transesterification between glycerol and methyl acetate among the tested H_(3)PW_(12)O_(40),Amber-lyst-45,HBEA,HZSM-5,ZrP,AlCl_(3) and FeCl_(3) catalysts.The calculated conversion of glycerol reached 81.3%with a 97.9%selectivity for monoacetin(MAG)and diacetin(DAG)with a 2.2%dosage of[H^(+)]at 100℃ for 4 h.The highest specific activity of PSA/K-ZrP-2 reached 24028.2 mg-glycerol/g-cat/h in a short reaction time(at 0.17 h),and it could be recycled five times without obvious deactivation.
基金This work was supported by the funds from the Medical and Health Science and Technology Development Project of Shandong Province,China(grant nos.2015WS0375 and 2019WS125)Scientific and Technological Project of Henan Province,China(grant nos.192102310114 and 192102310110).
文摘Objective:to explore the changes of the F-wave in the posterior tibial nerve of rabbits after different levels of lumbar spinal cord ischaemic injury and its correlation with motor function and the extent of lumbar spinal cord pathological damage.Methods:thirty New Zealand rabbits were randomly divided into 6 groups.The control group(n=5)was used to exclude the influence of anaesthesia and surgery on the F-wave.Different levels of lumbar arteries were ligated in the five experimental groups(n=5).The F-wave was recorded to observe the changes in the acute phase of spinal cord ischaemia.The correlation between the changes of the F-wave in the acute reversible phase and the motor function of the spinal cord was analysed.Motor functions were assessed after surgery and 2 d after vascular ligation.The specimens were taken 2 d after ligation for histopathologic observation.Results:the results for the control group indicated that anaesthesia and surgery did not affect the F-wave results.There was no statistically significant difference in the F-wave amplitudes and latency before and after ligation in the 1 and 2 level ligation groups.The F-wave changed immediately after ligation in the 3,4 and 5 ligation groups.The latency of the F-wave gradually extended,the amplitude of the F-wave gradually reduced.The amplitude variations of the F-wave were positively correlated with the motor function 2 d after ligation,there was a statistically significant difference.With the increase in the number of vascular ligation,the degree of destruction of the motor neurons in the anterior horn of the spinal cord in the pathological specimens increased.Conclusion:the F-waves in the posterior tibial nerve of rabbits were found to be sensitive to the lumbar spinal cord ischaemic injury and specific to predict motor function.
基金funded by the National Natural Science Foundation of China(Nos.82270033 and 81873407)the Natural Science Foundation of Hunan province(No.2022JJ30924)
文摘To the Editor:Allergic bronchopulmonary aspergillosis(ABPA)is an allergic lung disease caused by hypersensitivity to antigens from Aspergillus,and it most commonly occurs in patients with asthma or cysticbrosis.[1]The main clinical characteristics of the disease are those related to elevated serum immunoglubulin(Ig)E level,positive Aspergillus fumigatus(A.fumigatus)specic IgE/IgG,repeated pulmonary inltration,and bronchiectasis.However,due to the lack of unied diagnostic criteria and screening methods,ABPA is always underdiagnosed.In 2021,new diagnostic criteria were proposed for allergic bronchopulmonary mycosis(ABPM)in the Japanese population.
基金supported by the National Natural Science Foundation of China(Nos.52350411 and 52122507)the National Key R&D Program of China(No.2023YFB3405500)+1 种基金Sichuan Science and Technology Program(Nos.2023NSFSC1988 and 23NSFTD0030)the Independent Project of State Key Laboratory of Traction Power(No.2023TPL-T04).
文摘Lubrication failure accompanying with blackening phenomenon significantly reduces the long-running operational reliability of porous polymide(PPI)lubricated with poly-α-olefin(PAO)oil.Here,the effects of lubrication condition and counter-surface chemistry on the blackening failure of PAO impregnated PPI were studied through the comparison of the tribological tests against GCr15 steel ball and Al_(2)O_(3)ceramic ball with and without PAO oil lubrication.Black products were found to be formed on the PAO impregnated PPI surface slid against steel ball or Al_(2)O_(3)ball added with iron nano-particles,but be absent under the conditions without iron or PAO oil.Further analysis indicated that the iron-catalyzed splitting of PAO oil into small molecule alkanes and following the formation of black organic matter should be mainly responsible for the blackening phenomenon.Molecular dynamic(MD)simulations demonstrated that the iron facilitated the separation of hydrogen atom and the following broken of C–C bonds in PAO molecules,final resulting in the splitting of PAO oil.
基金supported by grants from the National Key Research and Development Program of China(grant no.2023YFD1202905).
文摘Cas12a(Cpf1),a Class 2 Type V CRISPR/Cas nuclease,has several unique attributes for genome editing and may provide a valuable alternative to Cas9.However,a low editing efficiency due to temperature sensitivity and insufficient cleavage activity of the Cas12a nuclease are major obstacles to its broad application.In this report,we generated two variants,ttAsCas12 Ultra and ttLbCas12a Ultra harboring three(E174R,M537R,and F870L)or two(D156R and E795L)mutations,respectively,by combining the mutations from the temperature-tolerant variants ttAsCas12a(E174R)and ttLbCas12a(D156R),and those from the highly active variants AsCas12a Ultra(M537R and F870L)and LbCas12a Ultra(E795L).We compared editing efficiencies of the five resulting Cas12a variants(LbCas12a,ttLbCas12a,ttLbCas12a Ultra,AsCas12a Ultra,and ttAsCas12 Ultra)at six target sites of four genes in Arabidopsis(Arabidopsis thaliana).The variant ttLbCas12a Ultra,harboring the D156R and E795L mutations,exhibited the highest editing efficiency of all variants tested in Arabidopsis and can be used to generate homozygous or biallelic mutants in a single generation in Arabidopsis plants grown at 22 C.In addition,optimization of ttLbCas12a Ultra,by varying nuclear localization signal sequences and codon usage,further greatly improved editing efficiency.Collectively,our results indicate that ttLbCas12a Ultra is a valuable alternative to Cas9 for editing genes or promoters in Arabidopsis.
基金supported by the National Key Research and Development Program of China(2022YFC3401802)the National Natural Science Foundation of China(32025001,32300021,22279105,and 32200017)+3 种基金the China Postdoctoral Science Foundation(2022M710080 and 2021M701989)the Postdoctoral Innovation Project of Shandong Province(SDCX-ZG-202201005)the China National Postdoctoral Program for Innovative Talents(BX20220191),the Shandong Provincial Natural Science Foundation(ZR2019ZD20,ZR2023QC021,and ZR2022QC070)Qingdao Natural Science Foundation(23-2-1-25-zyyd-jch)。
文摘Fuels and oleochemicals have been chemically produced fromabundant biological oils or fatty acids for more than a century,and modern biotechnology is now accelerating the advances infatty acid chemistry [1,2]. A number of enzymes responsible forthe biosynthesis of hydrocarbons from fatty acids have been discovered, providing a promising strategy for enzymatic synthesisof fatty hydrocarbons [3]. CYP152 peroxygenases have attracted agreat deal of attention due to their ability to one-step oxidativelydecarboxylate or hydroxylate fatty acids using H2O2 as sole oxidant, generating valuable a-olefins or hydroxylated fatty acids[4,5] (Fig. 1a). In addition to a-olefins as biofuels and chemicalintermediates, hydroxylated fatty acids are also an important classof compounds with various applications in food, cosmetic, pharmaceutical, and biomaterial industries [6,7] (Fig. 1b). Among them,P450BSb is considered as the most promising CYP152 peroxygenasefor practical applications due to its high stability and activity tocatalyze both b-hydroxylation (the preferred reaction) and decarboxylation (the side reaction) of fatty acids that are both mechanistically initiated by Cb-H abstraction. Of note, directed evolutioncould remodel its catalytic preference on decarboxylation orb-hydroxylation (e.g., the P450BSb-DC mutant with 67% decarboxylation selectivity) [5].
基金supported by the National Natural Science Foundation of China(22172063 and 21902062)Young Taishan Scholar Program(tsqn201812080)Independent Cultivation Program of Innovation Team of Ji’nan City(2021GXRC052).
文摘Concerns regarding human health and food safety have generated interest in developing simple,accurate,and cost-effective strategies for evaluating organophosphorus pesticide(OP)residues.In this study,nitrogen-doped carbon dots(N-CDs)were synthesized from a common low-cost precursor via a simple pyrolysis process.The fluorescence of the N-CDs can be suppressed by p-nitrophenol,which is the hydrolysis product of the p-nitrophenyl phosphate salt catalyzed by alkaline phosphatase(ALP).A fluorescent turn-on assay for the inhibitory effect of glyphosate was developed with a low detection limit and wide linear range.Moreover,the feasibility of visualizing OPs in vegetables was demonstrated by the fluorescence imaging of glyphosate on cabbage leaves.This research not only offers a facile method for the synthesis of highly fluorescent CDs but also inspires the development of effective multi-mode sensing platforms that include fluorescent testing and imaging for monitoring pesticide residues.
基金Supported by The Fourth Batch of High-end Talent Project in Hebei ProvinceAgricultural Science and Technology Achievement Promotion Project in Hebei Province(JNK 24083).
文摘In order to further promote the chestnut industrial chain,a large number of chestnut byproducts(chestnut leaves,chestnut shells,chestnut flowers,etc.)are produced every year.These chestnut byproducts will be comprehensively developed and utilized in different degrees,such as in-depth research and development in the fields of animal husbandry,agriculture,and cosmetics,so as to achieve the effects of accelerating the high-quality development of chestnut industry,realizing the green cycle of resources,reducing waste and promoting the development of rural industries.
文摘Regulating the interfacial charge transfer is pivotal for elucidating the kinetics of engineering the interface between the light-harvesting semiconductor and the substrate/catalyst for photoelectrocatalytic water splitting.In this study,we constructed a superior Ti-doped hematite photoanode(TiFeO)by employing SnOx as an electron transfer mediator,partially oxidized graphene(pGO)as a hole transfer mediator,and molecular Co cubane as a water oxidation catalyst.The Co/pGO/TiFeO/SnO_(x)integrated system achieves a photocurrent density of 2.52 mA cm–2 at 1.23 VRHE,which is 2.4 times higher than bare photoanode(1.04 mA cm^(-2)),with operational stability up to 100 h.Kinetic measurements indicate that pGO can promote charge transfer from TiFeO to the Co cubane catalyst.In contrast,SnOx reduces charge recombination at the interface between TiFeO and the fluorinated tin oxide substrate.In-situ infrared spectroscopy shows the formation of an O–O bonded intermediate during water oxidation.This study highlights the crucial role of incorporating dual charge-transfer mediators into photoelectrodes for efficient solar energy conversion.
