AIM:To compare the safety and efficacy of conbercept intravitreal injection and half-dose photodynamic therapy(PDT)in treating chronic central serous chorioretinopathy(CSC).METHODS:This study was retrospective.Thirty-...AIM:To compare the safety and efficacy of conbercept intravitreal injection and half-dose photodynamic therapy(PDT)in treating chronic central serous chorioretinopathy(CSC).METHODS:This study was retrospective.Thirty-seven patients(37 eyes)with chronic CSC received conbercept injections while 57 patients(57 eyes)were treated with half-dose PDT.All subjects were followed in 6mo.Outcome measures included change in best-corrected visual acuity(BCVA),central macular thickness(CMT),subfoveal choroidal thickness(SFCT),and resolution of subretinal fluid(SRF).RESULTS:There was no adverse event observed in either treatment group.At the 6-month follow-up,26 eyes(70.3%)in the conbercept group and 54 eyes(94.7%)in the half-dose PDT group(P<0.05)reached full resolution of SRF.The mean logarithm of the minimum angle of resolution(log MAR)BCVA significantly improved(P<0.001)in both treatment groups with better outcome at early phase in the half-dose PDT group(2 wk,1,and 2 mo,P<0.05).All subjects experienced significant CMT improvement(P<0.001)with no statistical difference between the two groups(P>0.05).The SFCT also improved in all subjects(P<0.001)with better outcome in the half-dose PDT group(P<0.05).CONCLUSION:Both intravitreal conbercept and halfdose PDT are safe to use in treating chronic CSC.By 6mo,both treatment groups are efficacious in improving BCVA,reducing CMT and SFCT,and resolving SRF in eyes with chronic CSC.Half-dose PDT may show better outcome at initial phase of treatment in chronic CSC.Longer follow-up period is necessary to study for long-term effect and safety.展开更多
BACKGROUND The expression of jumonji domain-containing 3(Jmjd3)and trimethylated H3 lysine 27(H3K27me3)in active ulcerative colitis(UC)and the correlation between vitamin D receptor(VDR)and the Jmjd3 pathway are unkno...BACKGROUND The expression of jumonji domain-containing 3(Jmjd3)and trimethylated H3 lysine 27(H3K27me3)in active ulcerative colitis(UC)and the correlation between vitamin D receptor(VDR)and the Jmjd3 pathway are unknown.AIM To study the relationship between VDR,Jmjd3 and H3K27me3 in patients with active UC.METHODS One hundred patients with active UC and 56 healthy controls were enrolled in this study.The patients with active UC were divided into groups according to mild(n=29),moderate(n=32)and severe(n=29)disease activity based on the modified Mayo score.Vitamin D levels were measured by radioimmunoassay.Colonic mucosal tissues from UC patients and controls were collected by colonoscopy.The expression of VDR,Jmjd3 and H3K27me3 in the intestinal mucosa was determined by immunohistochemistry staining.RESULTS Patients with active UC had lower levels of serum vitamin D(13.7±2.8 ng/mL,P<0.001)than the controls(16.2±2.5 ng/mL).In the UC cohort,serum vitamin D level was negatively correlated with disease activity(r=-0.323,P=0.001).VDR expression in the mucosa of UC patients was reduced compared to that in normal tissues(P<0.001)and negatively correlated with disease activity(r=-0.868,P<0.001).Similar results for VDR expression were noted in the most serious lesion(defined as UC diseased)and 20 cm proximal to the anus(defined as UC normal)(P<0.05).Simultaneously,Jmjd3 expression significantly increased in UC patients(P<0.001),but no difference was found between the different sites in UC patients.H3K27me3 expression in UC patients was significantly down-regulated when compared with normal tissues(P<0.001),but up-regulated in the mild disease activity group in comparison with the moderate disease activity group of UC patients(P<0.05).Jmjd3 Level was negatively correlated with the level of VDR(r=-0.342,P=0.002)and H3K27me3(r=-0.341,P=0.002),while VDR level was positively correlated with H3K27me3(r=0.473,P<0.001).CONCLUSION Serum vitamin D and VDR were inversely correlated with disease activity in active UC.Jmjd3 expression increased in the colonic mucosa of active UC patients and was negatively associated with VDR and H3K27me3 level.展开更多
BACKGROUND:Previous studies have reported inconsistent findings regarding the association between catestatin and outcomes of acute myocardial infarction(AMI).This study aims to investigate the prognostic value of cate...BACKGROUND:Previous studies have reported inconsistent findings regarding the association between catestatin and outcomes of acute myocardial infarction(AMI).This study aims to investigate the prognostic value of catestatin for long-term outcomes in patients with AMI.METHODS:One hundred and sixty-five patients with AMI were enrolled in this series.The plasma catestatin levels at baseline and clinical data were collected.All patients were followed up for four years to investigate whether there were major adverse cardiovascular events(MACEs),including cardiovascular death,recurrent AMI,rehospitalization for heart failure,and revascularization.RESULTS:There were 24 patients who had MACEs during the follow-up period.The MACEs group had significantly lower plasma catestatin levels(0.74±0.49 ng/m L vs.1.10±0.79 ng/m L,P=0.033)and were older(59.0±11.4 years old vs.53.2±12.8 years old,P=0.036).The rate of MACEs was significantly higher in the elderly group(≥60 years old)than in the young group(<60 years old)(23.8%[15/63]vs.8.8%[9/102],P=0.008).The catestatin level was significantly lower in the MACEs group than that in the non-MACEs group(0.76±0.50 ng/m L vs.1.31±0.77 ng/m L,P=0.012),and catestatin was significantly associated with MACEs(Kaplan Meier,P=0.007)among the elderly group,but not in the young group(Kaplan Meier,P=0.893).In the Cox proportional hazards regression,high catestatin was one of the independent factors for predicting MACEs after adjustment for other risk factors(hazard ratio 0.19,95%confidence interval 0.06–0.62,P=0.006)among elderly patients.CONCLUSIONS:Elderly AMI patients with lower plasma catestatin levels are more likely to develop MACEs.Catestatin may be a novel marker for the long-term prognosis of AMI,especially in elderly patients.展开更多
BACKGROUND Sensitive,novel,and accurate biomarkers for the detection of physiological changes in type 2 diabetes(T2DM)at an early stage are urgently needed.AIM To build a multi-parameter diagnostic model for the early...BACKGROUND Sensitive,novel,and accurate biomarkers for the detection of physiological changes in type 2 diabetes(T2DM)at an early stage are urgently needed.AIM To build a multi-parameter diagnostic model for the early detection of T2DM.METHODS MiR-148b,miR-223,miR-130a,and miR-19a levels were detected by real-time polymerase chain reaction in serum of healthy controls,individuals with impaired glucose regulation,and T2DM patients.The diagnostic value of miR-148b,miR-223,miR-130a,and miR-19a,alone or in combination,was analyzed.RESULTS The area under the curve(AUC)of miR-223,which had the best diagnostic value for discriminating the impaired glucose regulation and T2DM groups,was 0.84,and the sensitivity and specificity were 73.37%and 81.37%,respectively.The AUC of the four-miRNA signature was 0.90,and the sensitivity and specificity were 78.82%and 88.23%,respectively.In the validation set,the AUC was 0.88,and the sensitivity and specificity were 78.36%and 87.63%,respectively.CONCLUSION In summary,we have built a multi-parameter diagnostic model consisting of miR-148b,miR-223,miR-130a,and miR-19a for the detection of T2DM.It may be a potential tool for the early detection of T2DM.展开更多
Acute obstruction of the right coronary ar-tery(RCA)can lead to right ventricular myocardial infarction(MI),which rarely lead to cardiogenic shock(CS).Hemodynamic sta-bility could be restored through fluid resuscitati...Acute obstruction of the right coronary ar-tery(RCA)can lead to right ventricular myocardial infarction(MI),which rarely lead to cardiogenic shock(CS).Hemodynamic sta-bility could be restored through fluid resuscitation and vasoactive drugs in most circumstances.Here,we present a highly representative case of refractory CS caused by massive right ventricular MI with api-cal aneurysm formation,who was successfully treated with extracorporeal membrane oxygenation(ECMO)post severe complication of acute right coronal dis-section.展开更多
Evidence from animal experiments has shown that chlorinated polyfluoroalkyl ether sulfonic acids(Cl-PFESAs)can induce vision dysfunction in zebrafish.However,environmental epidemiological evidence supporting this hypo...Evidence from animal experiments has shown that chlorinated polyfluoroalkyl ether sulfonic acids(Cl-PFESAs)can induce vision dysfunction in zebrafish.However,environmental epidemiological evidence supporting this hypothesis remains limited.In our case−control study,samples collected from 270 individuals(135 controls and 135 cases)from the Isomers of C8 Health Project data were analyzed for Cl-PFESAs.We also repeated our analysis on zebrafish to support our findings in humans and to decipher the mechanism underlying Cl-PFESA eye toxicity.The serum levels of per-and polyfluoroalkyl substances(PFASs)and alternatives were significantly higher in the cases than in the controls.Higher serum Cl-PFESA levels were associated with greater odds of eye diseases,and the trend showed a statistically significant dose-dependent relationship.The Shapley additive explanations(SHAP)value indicated that 8:2 Cl-PFESA was the dominant eye disease risk factor among the 13 studied PFASs.In zebrafish experiments,Cl-PFESAs induced eye toxicity in adult zebrafish by oxidative damage and cell apoptosis.Compared to the control group,there was significantly reduced thicknesses of the inner plexiform layer(IPL),outer plexiform layer(OPL),and retinal tissue in the zebrafish exposed to Cl-PFESAs.Our study provides human clinical and animal experimental data,showing that exposure to PFASs increases the odds of the development of eye toxicity.展开更多
基金Supported by Wenzhou Municipal Science and Technology Bureau(No.Y20180728)Wenzhou Municipal Science and Technology Bureau(No.Y20190635)Wenzhou Municipal Science and Technology Bureau(No.2019Y0592)。
文摘AIM:To compare the safety and efficacy of conbercept intravitreal injection and half-dose photodynamic therapy(PDT)in treating chronic central serous chorioretinopathy(CSC).METHODS:This study was retrospective.Thirty-seven patients(37 eyes)with chronic CSC received conbercept injections while 57 patients(57 eyes)were treated with half-dose PDT.All subjects were followed in 6mo.Outcome measures included change in best-corrected visual acuity(BCVA),central macular thickness(CMT),subfoveal choroidal thickness(SFCT),and resolution of subretinal fluid(SRF).RESULTS:There was no adverse event observed in either treatment group.At the 6-month follow-up,26 eyes(70.3%)in the conbercept group and 54 eyes(94.7%)in the half-dose PDT group(P<0.05)reached full resolution of SRF.The mean logarithm of the minimum angle of resolution(log MAR)BCVA significantly improved(P<0.001)in both treatment groups with better outcome at early phase in the half-dose PDT group(2 wk,1,and 2 mo,P<0.05).All subjects experienced significant CMT improvement(P<0.001)with no statistical difference between the two groups(P>0.05).The SFCT also improved in all subjects(P<0.001)with better outcome in the half-dose PDT group(P<0.05).CONCLUSION:Both intravitreal conbercept and halfdose PDT are safe to use in treating chronic CSC.By 6mo,both treatment groups are efficacious in improving BCVA,reducing CMT and SFCT,and resolving SRF in eyes with chronic CSC.Half-dose PDT may show better outcome at initial phase of treatment in chronic CSC.Longer follow-up period is necessary to study for long-term effect and safety.
基金Supported by The Key Projects of Natural Science Research of Anhui Province in China,No.201904a07020043.
文摘BACKGROUND The expression of jumonji domain-containing 3(Jmjd3)and trimethylated H3 lysine 27(H3K27me3)in active ulcerative colitis(UC)and the correlation between vitamin D receptor(VDR)and the Jmjd3 pathway are unknown.AIM To study the relationship between VDR,Jmjd3 and H3K27me3 in patients with active UC.METHODS One hundred patients with active UC and 56 healthy controls were enrolled in this study.The patients with active UC were divided into groups according to mild(n=29),moderate(n=32)and severe(n=29)disease activity based on the modified Mayo score.Vitamin D levels were measured by radioimmunoassay.Colonic mucosal tissues from UC patients and controls were collected by colonoscopy.The expression of VDR,Jmjd3 and H3K27me3 in the intestinal mucosa was determined by immunohistochemistry staining.RESULTS Patients with active UC had lower levels of serum vitamin D(13.7±2.8 ng/mL,P<0.001)than the controls(16.2±2.5 ng/mL).In the UC cohort,serum vitamin D level was negatively correlated with disease activity(r=-0.323,P=0.001).VDR expression in the mucosa of UC patients was reduced compared to that in normal tissues(P<0.001)and negatively correlated with disease activity(r=-0.868,P<0.001).Similar results for VDR expression were noted in the most serious lesion(defined as UC diseased)and 20 cm proximal to the anus(defined as UC normal)(P<0.05).Simultaneously,Jmjd3 expression significantly increased in UC patients(P<0.001),but no difference was found between the different sites in UC patients.H3K27me3 expression in UC patients was significantly down-regulated when compared with normal tissues(P<0.001),but up-regulated in the mild disease activity group in comparison with the moderate disease activity group of UC patients(P<0.05).Jmjd3 Level was negatively correlated with the level of VDR(r=-0.342,P=0.002)and H3K27me3(r=-0.341,P=0.002),while VDR level was positively correlated with H3K27me3(r=0.473,P<0.001).CONCLUSION Serum vitamin D and VDR were inversely correlated with disease activity in active UC.Jmjd3 expression increased in the colonic mucosa of active UC patients and was negatively associated with VDR and H3K27me3 level.
基金supported by the National Natural Science Foundation of China(81400319)。
文摘BACKGROUND:Previous studies have reported inconsistent findings regarding the association between catestatin and outcomes of acute myocardial infarction(AMI).This study aims to investigate the prognostic value of catestatin for long-term outcomes in patients with AMI.METHODS:One hundred and sixty-five patients with AMI were enrolled in this series.The plasma catestatin levels at baseline and clinical data were collected.All patients were followed up for four years to investigate whether there were major adverse cardiovascular events(MACEs),including cardiovascular death,recurrent AMI,rehospitalization for heart failure,and revascularization.RESULTS:There were 24 patients who had MACEs during the follow-up period.The MACEs group had significantly lower plasma catestatin levels(0.74±0.49 ng/m L vs.1.10±0.79 ng/m L,P=0.033)and were older(59.0±11.4 years old vs.53.2±12.8 years old,P=0.036).The rate of MACEs was significantly higher in the elderly group(≥60 years old)than in the young group(<60 years old)(23.8%[15/63]vs.8.8%[9/102],P=0.008).The catestatin level was significantly lower in the MACEs group than that in the non-MACEs group(0.76±0.50 ng/m L vs.1.31±0.77 ng/m L,P=0.012),and catestatin was significantly associated with MACEs(Kaplan Meier,P=0.007)among the elderly group,but not in the young group(Kaplan Meier,P=0.893).In the Cox proportional hazards regression,high catestatin was one of the independent factors for predicting MACEs after adjustment for other risk factors(hazard ratio 0.19,95%confidence interval 0.06–0.62,P=0.006)among elderly patients.CONCLUSIONS:Elderly AMI patients with lower plasma catestatin levels are more likely to develop MACEs.Catestatin may be a novel marker for the long-term prognosis of AMI,especially in elderly patients.
基金Supported by the National Key R&D Program of China,No.2016YFC0106604.
文摘BACKGROUND Sensitive,novel,and accurate biomarkers for the detection of physiological changes in type 2 diabetes(T2DM)at an early stage are urgently needed.AIM To build a multi-parameter diagnostic model for the early detection of T2DM.METHODS MiR-148b,miR-223,miR-130a,and miR-19a levels were detected by real-time polymerase chain reaction in serum of healthy controls,individuals with impaired glucose regulation,and T2DM patients.The diagnostic value of miR-148b,miR-223,miR-130a,and miR-19a,alone or in combination,was analyzed.RESULTS The area under the curve(AUC)of miR-223,which had the best diagnostic value for discriminating the impaired glucose regulation and T2DM groups,was 0.84,and the sensitivity and specificity were 73.37%and 81.37%,respectively.The AUC of the four-miRNA signature was 0.90,and the sensitivity and specificity were 78.82%and 88.23%,respectively.In the validation set,the AUC was 0.88,and the sensitivity and specificity were 78.36%and 87.63%,respectively.CONCLUSION In summary,we have built a multi-parameter diagnostic model consisting of miR-148b,miR-223,miR-130a,and miR-19a for the detection of T2DM.It may be a potential tool for the early detection of T2DM.
文摘Acute obstruction of the right coronary ar-tery(RCA)can lead to right ventricular myocardial infarction(MI),which rarely lead to cardiogenic shock(CS).Hemodynamic sta-bility could be restored through fluid resuscitation and vasoactive drugs in most circumstances.Here,we present a highly representative case of refractory CS caused by massive right ventricular MI with api-cal aneurysm formation,who was successfully treated with extracorporeal membrane oxygenation(ECMO)post severe complication of acute right coronal dis-section.
基金supported by the National Key Research and Development Program of China(2018YFC1004300,2018YFC1004301,and 2018YFE0106900)the National Natural Science Foundation of China(82173471,82003409,82103823,and 82073503)+1 种基金the Natural Science Foundation of Guangdong Province(2021A1515012212,2021A1515011754,2021B1515020015,2020A1515011131,2019A050510017,2018B05052007,and 2017A090905042)the Guangxi Key Research and Development Plan(GUIKEAB18050024).
文摘Evidence from animal experiments has shown that chlorinated polyfluoroalkyl ether sulfonic acids(Cl-PFESAs)can induce vision dysfunction in zebrafish.However,environmental epidemiological evidence supporting this hypothesis remains limited.In our case−control study,samples collected from 270 individuals(135 controls and 135 cases)from the Isomers of C8 Health Project data were analyzed for Cl-PFESAs.We also repeated our analysis on zebrafish to support our findings in humans and to decipher the mechanism underlying Cl-PFESA eye toxicity.The serum levels of per-and polyfluoroalkyl substances(PFASs)and alternatives were significantly higher in the cases than in the controls.Higher serum Cl-PFESA levels were associated with greater odds of eye diseases,and the trend showed a statistically significant dose-dependent relationship.The Shapley additive explanations(SHAP)value indicated that 8:2 Cl-PFESA was the dominant eye disease risk factor among the 13 studied PFASs.In zebrafish experiments,Cl-PFESAs induced eye toxicity in adult zebrafish by oxidative damage and cell apoptosis.Compared to the control group,there was significantly reduced thicknesses of the inner plexiform layer(IPL),outer plexiform layer(OPL),and retinal tissue in the zebrafish exposed to Cl-PFESAs.Our study provides human clinical and animal experimental data,showing that exposure to PFASs increases the odds of the development of eye toxicity.
