目的比较腹腔镜与开腹手术治疗中国老年宫颈癌的疗效。方法计算机检索中国生物医学文摘数据库(CBM)、万方全文数据库(wanfangdata)、中国知网全文数据库(CNKI)、维普中文期刊数据库(VIP)和Pub Med,根据入选标准筛选文献。从文献提取数据...目的比较腹腔镜与开腹手术治疗中国老年宫颈癌的疗效。方法计算机检索中国生物医学文摘数据库(CBM)、万方全文数据库(wanfangdata)、中国知网全文数据库(CNKI)、维普中文期刊数据库(VIP)和Pub Med,根据入选标准筛选文献。从文献提取数据,采用Rev Man 5.2.9软件进行统计学分析。结果最终纳入符合标准的文献10篇,均为临床对照研究(CCT),无随机对照研究(RCT)。腹腔镜手术组与开腹手术组患者年龄、体重、肿瘤分期等基本特征无明显差异。与开腹手术相比,腹腔镜手术组手术时间长(MD=32.60,95%CI:5.65~59.55,P=0.020),术中失血量少(MD=-94.01,95%CI:-130.65^-57.37,P=0.000),清除淋巴结数量少(MD=1.69,95%CI:0.67~2.72,P=0.001),术后排气时间早(MD=-17.09,95%CI:-21.19^-12.98,P=0.000),住院时间短(MD=-4.30,95%CI:-5.57^-3.02,P=0.000),差异有统计学意义。而术后留置尿管时间(MD=-0.67,95%CI:-1.92~0.58,P=0.290)、手术并发症发生率(OR^=0.62,95%CI:0.27~1.42,P=0.260),差异无统计学意义。结论腹腔镜手术具有创伤小、恢复快等优势,并不增加手术并发症发生率,是治疗中国老年宫颈癌的理想术式。展开更多
The mortality rate of acute severe intraventricular hematoma is extremely high, and the rate of disability in survivors is high. Intraventricular hematoma has always been a difficult problem for clinical treatment. Al...The mortality rate of acute severe intraventricular hematoma is extremely high, and the rate of disability in survivors is high. Intraventricular hematoma has always been a difficult problem for clinical treatment. Although minimally invasive endoscopic hematoma evacuation is widely used to treat this disease, the technique still has room for improvement. Equipment for the intra-neuroendoscopic technique（INET） consists of two of our patented inventions： a transparent sheath（Patent No. ZL 200820046232.0） and a hematoma aspirator（Patent No. ZL 201520248717.8）. This study explored the safety and efficacy of INET by comparing it with extraventricular drainage in combination with urokinase thrombolytic therapy. This trial recruited 65 patients with severe intraventricular hemorrhage, including 35（19 men and 16 women, aged 53.2 ± 8.7 years） in the INET group and 30（17 men and 13 women, aged 51.5 ± 7.9 years） in the control group（extraventricular drainage plus urokinase thrombolytic therapy）. Our results showed that compared with the control group, the INET group exhibited lower intraventricular hemorrhage volumes, shorter intensive care-unit monitoring and ventricular drainage-tube placement times, and fewer incidences of intracranial infection, secondary bleeding, and mortality. Thus, the prognosis of survivors had improved remarkably. These findings indicate that INET is a safe and efficient new method for treating severe intraventricular hematoma. This trial was registered with Clinical Trials.gov（NCT02515903）.展开更多
Since the late 2010s,Artificial Intelligence(AI)including machine learning,boosted through deep learning,has boomed as a vital tool to leverage computer vision,natural language processing and speech recognition in rev...Since the late 2010s,Artificial Intelligence(AI)including machine learning,boosted through deep learning,has boomed as a vital tool to leverage computer vision,natural language processing and speech recognition in revolutionizing zoological research.This review provides an overview of the primary tasks,core models,datasets,and applications of AI in zoological research,including animal classification,resource conservation,behavior,development,genetics and evolution,breeding and health,disease models,and paleontology.Additionally,we explore the challenges and future directions of integrating AI into this field.Based on numerous case studies,this review outlines various avenues for incorporating AI into zoological research and underscores its potential to enhance our understanding of the intricate relationships that exist within the animal kingdom.As we build a bridge between beast and byte realms,this review serves as a resource for envisioning novel AI applications in zoological research that have not yet been explored.展开更多
BACKGROUND:Individuals who survive a cardiac arrest often sustain cognitive impairments due to ischemia-reperfusion injury.Mesenchymal stem cell(MSC)transplantation is used to reduce tissue damage,but exosomes are mor...BACKGROUND:Individuals who survive a cardiac arrest often sustain cognitive impairments due to ischemia-reperfusion injury.Mesenchymal stem cell(MSC)transplantation is used to reduce tissue damage,but exosomes are more stable and highly conserved than MSCs.This study was conducted to investigate the therapeutic effects of MSC-derived exosomes(MSC-Exo)on cerebral ischemia-reperfusion injury in an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R),and to explore the underlying mechanisms.METHODS:Primary hippocampal neurons obtained from 18-day Sprague-Dawley rat embryos were subjected to OGD/R treatment,with or without MSC-Exo treatment.Exosomal integration,cell viability,mitochondrial membrane potential,and generation of reactive oxygen species(ROS)were examined.Terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nickend labeling(TUNEL)staining was performed to detect neuronal apoptosis.Moreover,mitochondrial function-associated gene expression,Nrf2 translocation,and expression of downstream antioxidant proteins were determined.RESULTS:MSC-Exo attenuated OGD/R-induced neuronal apoptosis and decreased ROS generation(P<0.05).The exosomes reduced OGD/R-induced Nrf2 translocation into the nucleus(2.14±0.65 vs.5.48±1.09,P<0.01)and increased the intracellular expression of antioxidative proteins,including superoxide dismutase and glutathione peroxidase(17.18±0.97 vs.14.40±0.62,and 20.65±2.23 vs.16.44±2.05,respectively;P<0.05 for both).OGD/R significantly impaired the mitochondrial membrane potential and modulated the expression of mitochondrial functionassociated genes,such as PINK,DJ1,LRRK2,Mfn-1,Mfn-2,and OPA1.The abovementioned changes were partially reversed by exosomal treatment of the hippocampal neurons.CONCLUSIONS:MSC-Exo treatment can alleviate OGD/R-induced oxidative stress and dysregulation of mitochondrial function-associated genes in hippocampal neurons.Therefore,MSCExo might be a potential therapeutic strategy to prevent OGD/R-induced neuronal injury.展开更多
The structural, mechanical and electronic properties of W_(1-x)Zr_x(x=0.0625, 0.125, 0.1875, 0.25, 0.5) are systematically investigated by means of first-principles calculation. The total-energy calculations demonstra...The structural, mechanical and electronic properties of W_(1-x)Zr_x(x=0.0625, 0.125, 0.1875, 0.25, 0.5) are systematically investigated by means of first-principles calculation. The total-energy calculations demonstrate that the W–Zr binary substitutional solid solution remaining bcc structure can be formed at an atom level. In addition, the derived bulk modulus(B), shear modulus(G), Young's modulus(E) for each of W–Zr alloys decrease gradually with the increase of Zr concentration, suggesting that W alloying with higher Zr concentration becomes softer than pure W metal. Based on the mechanical characteristic B/G ratio, Poisson's ratio υ and Cauchy pressure C, all W_(1-x)Zr_x alloys are regarded as ductile materials. The ductility for each of those materials is improved with the increase of Zr concentration. The calculated density of states indicates that the ductility of W_(1-x)Zr_x is due to the fact that the bonding in the alloy becomes more metallic through increasing the Zr concentration in tungsten. These results provide incontrovertible evidence for the fact that Zr has a significant influence on the properties of W.展开更多
OBJECTIVE This study aimed to explore the expression and significance of transforming growth factor β1（TGF-β1）,extracellular signal-regulated kinases 1/2 （ERK1/2）, and K-ras in colorectal cancer （CRC） using ti...OBJECTIVE This study aimed to explore the expression and significance of transforming growth factor β1（TGF-β1）,extracellular signal-regulated kinases 1/2 （ERK1/2）, and K-ras in colorectal cancer （CRC） using tissue microarray technology.METHODS The expressions of TGF-β1, ERK1/2, and K-ras in colon cancer cells taken from the specimens of 92 CRC patients （stage Ⅰ： 16 cases, stage Ⅱ： 28 cases, stage Ⅲ： 24 cases, and stage Ⅳ：24 cases） were analyzed using tissue microarray technology and immunohistochemistry, and compared with those of 20 normal colon tissue samples.RESULTS High immunoreactive scores （IRS） of TGF-β1,p-ERK1/2, and K-ras protein in CRC were obtained, which were 66.3% （61/92）, 59.8% （55/92）, and 48.9% （45/92）, respectively, and those in normal epithelial cells of colon were 10% （2/20）, 20% （4/20）, and 30% （6/20）, respectively （P 〈 0.05）. The expressions of TGF-β1 and ERK1/2 in CRC at stage Ⅰwere 37.5% and 31.3%,respectively, and those in CRC at stage Ⅳ were 83.3% and79.3%, respectively, with statistically significant differences. No significant relationship was found between K-ras expression and tumor stages （P〉0.05）.CONCLUSION High level expressions of TGF-β1 and ERK1/2 are closely related to the clinical stages of colon cancer and crosstalk may exist between the 2 signal pathways.展开更多
Charged amino acids (AAs) are targets for selective forces in protein evolution. To fully explore the trend of charged AA frequencies evolution in macroevolutionary process from prokaryotes to eukaryotes, we extend th...Charged amino acids (AAs) are targets for selective forces in protein evolution. To fully explore the trend of charged AA frequencies evolution in macroevolutionary process from prokaryotes to eukaryotes, we extend the analysis of five charged AAs separately and total basic and acidic AAs in protein sequences of 158 prokaryotic and 63 eukaryotic predicted proteomes and 456 clusters of orthologous groups (COGs). Also, we eliminate the biases that may caused by extreme organisms in both predicted proteomes and COGs analyses. More basic AAs, His,Lysand Glu were found in eukaryotic proteins compared with prokaryotic proteins by predicted proteomes analysis. By COGs analysis, we found that basic AAs andLysfrequencies are higher in eukaryotic orthologous proteins than their prokaryotic companions, while the trend of Arg frequency is the opposite. We discussed the agreements and disagreements of two analyses and gained a more credible trend of charged AAs evolution in macroevolutionary time scale.展开更多
Objective: To investigate the effects of laparoscopic surgery and open surgery on serum tumor markers, coagulation function and immune function in patients with early endometrial cancer. Methods: The clinical data of ...Objective: To investigate the effects of laparoscopic surgery and open surgery on serum tumor markers, coagulation function and immune function in patients with early endometrial cancer. Methods: The clinical data of 82 cases of early endometrial carcinoma were retrospectively analyzed, and they were divided into control group (n=40) and observation group (n=42) according to the treatment plan. Patients in the control group were treated with open surgery, and the observation group received laparoscopic treatment. The levels of serum tumor markers, coagulation function and immune function indexes of the two groups were compared between before operation and 1 d after operation. Results: The levels of serum CA-125, HE4, PT, APTT, FIB, CD3+, CD4+, CD8+ and CD4+/CD8+in the two groups before treatment were not significantly different. After treatment, the levels of CA-125, HE4, PT, APTT, CD3+, CD4+and CD4+/CD8+ in the two groups were significantly lower than those before treatment in the same group, and the levels of CA-125, HE4, PT and APTT in the observation group were significantly lower than those in the control group, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than those of the control group;After treatment, the levels of FIB in the two groups were significantly higher than that before treatment, and the level of observation group was significantly higher than that of the control group. Conclusion: Compared with open surgery for early endometrial cancer, laparoscopic surgery can effectively reduce the level of serum tumor markers and the inhibition of immune function, however, it has great influence on coagulation function, and effective measures should be taken to prevent the formation of thrombotic diseases.展开更多
Background:As a disease,insomnia is often ignored by the general public.Insomnia,which not directly fatal,may be equivalent to chronic suicide if it is not paid attention to.However,there are many theories on how to t...Background:As a disease,insomnia is often ignored by the general public.Insomnia,which not directly fatal,may be equivalent to chronic suicide if it is not paid attention to.However,there are many theories on how to treat insomnia,and researchers have been searching for a cure.Chinese herbal medicine(CHM)and exercise therapy(ET)are relatively effective,and have been used since ancient times to treat insomnia.This study aimed to examine the effect of CHM combined with ET(CHM-ET)on patients with insomnia.Methods:We searched eight electronic databases including PubMed,Web of Science,EMBASE,the Cochrane Library,the China National Knowledge Infrastructure(CNKI),the China Biology Medicine disc(CBMdisc),the China Science and Technology Journal Database(VIP),the Wanfang Database to find randomized controlled trials(RCTs)evaluating CHM-ET in the treatment of insomnia patients up to September 13,2022.Two researchers read and screened the publications to extract data.We used the pittsburgh sleep quality index(PSQI)as the evaluation indicators for each study,and the other was the total effective rate(TER).The Cochrane risk-of-bias assessment tool was used to assess the risk of bias of the included literature.The level of evidence for this result was assessed by GARDE method.The meta-analysis was performed using STATA 14 software and RevMan 5.3.The research method was registered with PROSPERO(CRD42022350926).Results:We included fourteen randomized controlled trials,which including a total of 1,126 participants.Compared with the control group,the experimental group showed significantly improvements in sleep effect as reflected by the reduced PSQI score[mean difference(MD)=-2.47,95%confidence interval(CI)(-3.15,-1.78),I^(2)=92%]with low quality of evidence,and increased TER[risk ratio(RR)=1.23,95%CI(1.14,1.33),I^(2)=40%]with moderate quality of evidence.Compared with hypnotic drugs,CHM-ET significantly reduced the PSQI score[mean difference(MD)=-3.18,95%CI(-5.48,-0.89),I^(2)=73%]with low quality of evidence.The PSQI of CHM-ET significantly decreased compared with single CHM[mean difference(MD)=-3.04,95%CI(-5.84,-0.25),I^(2)=98%]with low quality of evidence,and ET[mean difference(MD)=-2.44,95%CI(-2.87,-2.02),I^(2)=0%]with moderate quality of evidence.No serious adverse reactions were observed.Conclusion:This review suggested that CHM-ET may be an effective treatment for insomnia.However,given the limited quality of the studies and methodologies included in the trials,further rigorous randomized controlled trials are needed for more accurate results.展开更多
Anopheles sinensis is a major malaria vector. Insect odorant-binding proteins （OBPs） may function in the reception of odorants in the olfactory system. The classification and characterization of the An. sinensis OBP...Anopheles sinensis is a major malaria vector. Insect odorant-binding proteins （OBPs） may function in the reception of odorants in the olfactory system. The classification and characterization of the An. sinensis OBP genes have not been systematically studied. In this study, 64 putative OBP genes were identified at the whole-genome level of An. sinensis based on the comparison between OBP conserved motifs, PBP_GOBE and phylogenetic analysis with An. gambiae OBPs. The characterization of An. sinensis OBPs, including the motifs conservation, gene structure, genomic organization and classification, were investigated. A new gene, AsOBP73, belonging to the Plus-C subfamily, was identified with the support of transcript and conservative motifs. These An. sinensis OBP genes were classified into three subfamilies with 37, 15 and 12 genes in the subfamily Classic, Atypical and Plus-C, respectively. The genomic organization of An. sinensis OBPs suggests a clustered distribution across nine different scaffolds. Eight genes （0BP23-28, 0BP63- 64） might originate from a single gene through a series of historic duplication events at least before divergence of Anopheles, Culex and Aedes. The microsynteny analyses indicate a very high synteny between An. sinensis and An. gambiae OBPs. OBP70 and OBP71 earlier classified under Plus-C in An. gambiae are recognized as belonging to the group Obp59a of the Classic subfamily, and OBP69 earlier classified under Plus-C has been moved to the Atypical subfamily in this study. The study established a basic information frame for further study of the OBP genes in insects as well as in An. sinensis.展开更多
The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic...The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause （SD）, high-throughput RNA-Seq data were generated from non-diapause （ND） and SD （initial, maintenance and quiescence phase） pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.展开更多
Simple sequence repeats (SSRs) exist in both eukaryotic and prokaryotic genomes and are the most popular genetic markers, but the SSRs of mosquito genomes are still not well understood. In this study, we identified an...Simple sequence repeats (SSRs) exist in both eukaryotic and prokaryotic genomes and are the most popular genetic markers, but the SSRs of mosquito genomes are still not well understood. In this study, we identified and analyzed the SSRs in 23 mosquito species using Drosophila melanogaster as reference at the whole-genome level. The results show that SSR numbers (33 076-560 175/genome) and genome sizes (574.57-1342.21 Mb) are significantly positively correlated (R~= 0.8992, P < 0.01), but the correlation in individual species varies in these mosquito species. In six types of SSR, mono- to trinucleotide SSRs are dominant with cumulative percentages of 95.14%-99.00% and densities of 195.65/Mb-787.51/Mb, whereas tetra- to hexanucleotide SSRs are rare with 1.12%-4.22% and 3.76/Mb-40.23/Mb. The (A/T)n,(AC/GT)n and (AGC/GCT)n are the most frequent motifs in mononucleotide, dinucleotide and trinucleotide SSRs, respectively, and the motif frequencies of tetra- to hexanucleotide SSRs appear to be species-specific. The 10-20 bp length of SSRs are dominant with the number of 11() 561 ± 93 482 and the frequency of 87.25%± 5.73% on average, and the number and frequency decline with the increase oflength. Most SSRs(83.34%± 7.72%) are located in intergenic regions, followed by intron regions (11.59%± 5.59%), exon regions (3.74%± 1.95%), and untranslated regions (1.32%± 1.39%). The mono-, di- and trinucleotide SSRs are the main SSRs in both gene regions (98.55%± 0.85%) and exon regions (99.27%± 0.52%). An average of 42.52% of total genes contains SSRs, and the preference for SSR occurrenee in different gene subcategories are species-specific. The study provides useful insights into the SSR diversity, characteristics and distribution in 23 mosquito species of genomes.展开更多
Hydrogen nuclear magnetic resonance(H1-NMR) and X-ray diffractometer(XRD) were used to characterize the molecular and crystal structure of lead methacrylate [Pb(MAA)_(2)] which was produced by the double decomposition...Hydrogen nuclear magnetic resonance(H1-NMR) and X-ray diffractometer(XRD) were used to characterize the molecular and crystal structure of lead methacrylate [Pb(MAA)_(2)] which was produced by the double decomposition reaction of lead oxide and methacrylic acid.Isothermal analysis and Kelen-T(u|")dos(K-T) method were used to study the self-polymerization kinetic and the monomer reactivity ratios of Pb(M A A)_(2) and methyl methacrylate(MMA),respectively.By the ternary polymerization method of bulk casting using MMA,Pb(MAA)_(2) and gadolinium methacrylate(Gd(MAA)_(3)) as monomers,we prepared the plexiglass which have neutron and X-ray protection property.The results show that the polymerization rate(R_(p)) is expressed as Rp=K[M]^(1.02)[I]^(0.37) below the 10% conversion rate at 70℃,where K is the polymerization rate constant.And in the N,N-dimethylformamide(DMF) solution,the activation energy required for Pb(MAA)_(2) to initiate self-polymerization by the free radicals is 74.99 kJ·mol^(-1).The reactivity ratios of r1 [Pb(MAA)_(2)] and r2(MMA) are 3.767 and0.166.As the thickness of the material increases,the X-ray and thermal neutron shielding ability of the plexiglass containing gadolinium and lead is getting better and better.展开更多
We investigate a continuous Heisenberg spin chain equation which models the local magnetization in ferromagnet with time-and site-dependent inhomogeneous bilinear interaction and timedependent spin-transfer torque.By ...We investigate a continuous Heisenberg spin chain equation which models the local magnetization in ferromagnet with time-and site-dependent inhomogeneous bilinear interaction and timedependent spin-transfer torque.By establishing the gauge equivalence between the spin chain equation and an integrable generalized nonlinear Schrödinger equation,we present explicitly a novel nonautonomous magnetic soliton solution for the spin chain equation.The results display how the dynamics of the magnetic soliton can be controlled by the bilinear interaction and spin-polarized current.Especially,we find that the site-dependent bilinear interaction may break some conserved quantity,and give rise to damping-like effect in the spin evolution.展开更多
Biological thiols（biothiols）, an important kind of functional biomolecules, such as cysteine（Cys） and glutathione（GSH）, play vital roles in maintaining the stability of the intracellular environment. In past dec...Biological thiols（biothiols）, an important kind of functional biomolecules, such as cysteine（Cys） and glutathione（GSH）, play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.展开更多
nc-Ti C/a-C：H nanocomposite films were prepared by filtered cathodic arc technique. The influence of C_2H_2/Ar flow ratio on the composition, structure, and mechanical properties of films was investigated by X-ray ph...nc-Ti C/a-C：H nanocomposite films were prepared by filtered cathodic arc technique. The influence of C_2H_2/Ar flow ratio on the composition, structure, and mechanical properties of films was investigated by X-ray photoelectron spectroscopy（XPS）, X-ray diffraction（XRD）, Fourier transform infrared spectroscopy（FTIR）,Raman spectroscopy, nanoindentation, and ball-on-disc tribometry. The films show a nanocomposite structure in which Ti C crystallites are embedded in the amorphous matrix of a-C：H phase. C content in films increases with the flow ratio of C_2H_2/Ar, simultaneously, the crystallite size of TiC decreases. Contrary to the nc-Ti C/a-C：H films deposited by magnetron sputtering in which the sp3 C content increases with C_2H_2 flow rate, the increase of C_2H_2 flow rate leads to the increase of sp2 C content in films deposited by filtered cathodic arc technique. The nc-Ti C/a-C：H films deposited by cathodic arc technique have a pronounced hardness maximum of 30 GPa under the C_2H_2/Ar flow ratio of 12. Tribological performance of films is controlled by the sp2 content in films. Higher sp2 content promotes the formation of graphite-like transfer layer during sliding,and results in lower wear rate and friction coefficient.展开更多
文摘目的比较腹腔镜与开腹手术治疗中国老年宫颈癌的疗效。方法计算机检索中国生物医学文摘数据库(CBM)、万方全文数据库(wanfangdata)、中国知网全文数据库(CNKI)、维普中文期刊数据库(VIP)和Pub Med,根据入选标准筛选文献。从文献提取数据,采用Rev Man 5.2.9软件进行统计学分析。结果最终纳入符合标准的文献10篇,均为临床对照研究(CCT),无随机对照研究(RCT)。腹腔镜手术组与开腹手术组患者年龄、体重、肿瘤分期等基本特征无明显差异。与开腹手术相比,腹腔镜手术组手术时间长(MD=32.60,95%CI:5.65~59.55,P=0.020),术中失血量少(MD=-94.01,95%CI:-130.65^-57.37,P=0.000),清除淋巴结数量少(MD=1.69,95%CI:0.67~2.72,P=0.001),术后排气时间早(MD=-17.09,95%CI:-21.19^-12.98,P=0.000),住院时间短(MD=-4.30,95%CI:-5.57^-3.02,P=0.000),差异有统计学意义。而术后留置尿管时间(MD=-0.67,95%CI:-1.92~0.58,P=0.290)、手术并发症发生率(OR^=0.62,95%CI:0.27~1.42,P=0.260),差异无统计学意义。结论腹腔镜手术具有创伤小、恢复快等优势,并不增加手术并发症发生率,是治疗中国老年宫颈癌的理想术式。
基金supported by the National Key R&D Program of China(No.2021YFE0102200)the Inner Mongolia Natural Science Foundation,China(No.2020MS05063)the Inner Mongolia Science and Technology Plan Project,China(No.2020GG0230)。
基金funded by a grant from the Clinical Research Project of Shenzhen Health and Family Planning Commission in China,No.SZLY2018007a grant from the Science and Technology Planning Project of Shenzhen City of China,No.JCYJ 20150403101028210+2 种基金a grant from the Science and Technology Development and Cultivation Project of Southern Medical University of China,No.KJ 20161115the Guangdong Provincial Medical Research Fund in China,No.A2016545the Shenzhen Health Planning Commission Research Fund in China,No.201601013,201506009
文摘The mortality rate of acute severe intraventricular hematoma is extremely high, and the rate of disability in survivors is high. Intraventricular hematoma has always been a difficult problem for clinical treatment. Although minimally invasive endoscopic hematoma evacuation is widely used to treat this disease, the technique still has room for improvement. Equipment for the intra-neuroendoscopic technique（INET） consists of two of our patented inventions： a transparent sheath（Patent No. ZL 200820046232.0） and a hematoma aspirator（Patent No. ZL 201520248717.8）. This study explored the safety and efficacy of INET by comparing it with extraventricular drainage in combination with urokinase thrombolytic therapy. This trial recruited 65 patients with severe intraventricular hemorrhage, including 35（19 men and 16 women, aged 53.2 ± 8.7 years） in the INET group and 30（17 men and 13 women, aged 51.5 ± 7.9 years） in the control group（extraventricular drainage plus urokinase thrombolytic therapy）. Our results showed that compared with the control group, the INET group exhibited lower intraventricular hemorrhage volumes, shorter intensive care-unit monitoring and ventricular drainage-tube placement times, and fewer incidences of intracranial infection, secondary bleeding, and mortality. Thus, the prognosis of survivors had improved remarkably. These findings indicate that INET is a safe and efficient new method for treating severe intraventricular hematoma. This trial was registered with Clinical Trials.gov（NCT02515903）.
基金supported by the National Natural Science Foundation of China (31871274)Natural Science Foundation of Chongqing,China (CSTB2022NSCQ-MSX0650)+2 种基金Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN202100508)Team Project of Innovation Leading Talent in Chongqing (CQYC20210309536)“Contract System”Project of Chongqing Talent Plan (cstc2022ycjh-bgzxm0147)。
文摘Since the late 2010s,Artificial Intelligence(AI)including machine learning,boosted through deep learning,has boomed as a vital tool to leverage computer vision,natural language processing and speech recognition in revolutionizing zoological research.This review provides an overview of the primary tasks,core models,datasets,and applications of AI in zoological research,including animal classification,resource conservation,behavior,development,genetics and evolution,breeding and health,disease models,and paleontology.Additionally,we explore the challenges and future directions of integrating AI into this field.Based on numerous case studies,this review outlines various avenues for incorporating AI into zoological research and underscores its potential to enhance our understanding of the intricate relationships that exist within the animal kingdom.As we build a bridge between beast and byte realms,this review serves as a resource for envisioning novel AI applications in zoological research that have not yet been explored.
基金supported by a grant from the National Natural Science Foundation of China(81701872)。
文摘BACKGROUND:Individuals who survive a cardiac arrest often sustain cognitive impairments due to ischemia-reperfusion injury.Mesenchymal stem cell(MSC)transplantation is used to reduce tissue damage,but exosomes are more stable and highly conserved than MSCs.This study was conducted to investigate the therapeutic effects of MSC-derived exosomes(MSC-Exo)on cerebral ischemia-reperfusion injury in an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R),and to explore the underlying mechanisms.METHODS:Primary hippocampal neurons obtained from 18-day Sprague-Dawley rat embryos were subjected to OGD/R treatment,with or without MSC-Exo treatment.Exosomal integration,cell viability,mitochondrial membrane potential,and generation of reactive oxygen species(ROS)were examined.Terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nickend labeling(TUNEL)staining was performed to detect neuronal apoptosis.Moreover,mitochondrial function-associated gene expression,Nrf2 translocation,and expression of downstream antioxidant proteins were determined.RESULTS:MSC-Exo attenuated OGD/R-induced neuronal apoptosis and decreased ROS generation(P<0.05).The exosomes reduced OGD/R-induced Nrf2 translocation into the nucleus(2.14±0.65 vs.5.48±1.09,P<0.01)and increased the intracellular expression of antioxidative proteins,including superoxide dismutase and glutathione peroxidase(17.18±0.97 vs.14.40±0.62,and 20.65±2.23 vs.16.44±2.05,respectively;P<0.05 for both).OGD/R significantly impaired the mitochondrial membrane potential and modulated the expression of mitochondrial functionassociated genes,such as PINK,DJ1,LRRK2,Mfn-1,Mfn-2,and OPA1.The abovementioned changes were partially reversed by exosomal treatment of the hippocampal neurons.CONCLUSIONS:MSC-Exo treatment can alleviate OGD/R-induced oxidative stress and dysregulation of mitochondrial function-associated genes in hippocampal neurons.Therefore,MSCExo might be a potential therapeutic strategy to prevent OGD/R-induced neuronal injury.
基金Project supported by the Beijing Municipal Natural Science Foundation,China(Grant No.2182042)the National Natural Science Foundation of China(Grant Nos.11875004,11505006,and 11604008)
文摘The structural, mechanical and electronic properties of W_(1-x)Zr_x(x=0.0625, 0.125, 0.1875, 0.25, 0.5) are systematically investigated by means of first-principles calculation. The total-energy calculations demonstrate that the W–Zr binary substitutional solid solution remaining bcc structure can be formed at an atom level. In addition, the derived bulk modulus(B), shear modulus(G), Young's modulus(E) for each of W–Zr alloys decrease gradually with the increase of Zr concentration, suggesting that W alloying with higher Zr concentration becomes softer than pure W metal. Based on the mechanical characteristic B/G ratio, Poisson's ratio υ and Cauchy pressure C, all W_(1-x)Zr_x alloys are regarded as ductile materials. The ductility for each of those materials is improved with the increase of Zr concentration. The calculated density of states indicates that the ductility of W_(1-x)Zr_x is due to the fact that the bonding in the alloy becomes more metallic through increasing the Zr concentration in tungsten. These results provide incontrovertible evidence for the fact that Zr has a significant influence on the properties of W.
基金This work was supported by grants from Soochow University Students Innovation Foundation （No. 5731512810）, Chinese National Natural Science Foundation （No.81072186）, and Jiangsu Provincial Higher Institution Natural Science Foundation （No.10KJB320018）.
文摘OBJECTIVE This study aimed to explore the expression and significance of transforming growth factor β1（TGF-β1）,extracellular signal-regulated kinases 1/2 （ERK1/2）, and K-ras in colorectal cancer （CRC） using tissue microarray technology.METHODS The expressions of TGF-β1, ERK1/2, and K-ras in colon cancer cells taken from the specimens of 92 CRC patients （stage Ⅰ： 16 cases, stage Ⅱ： 28 cases, stage Ⅲ： 24 cases, and stage Ⅳ：24 cases） were analyzed using tissue microarray technology and immunohistochemistry, and compared with those of 20 normal colon tissue samples.RESULTS High immunoreactive scores （IRS） of TGF-β1,p-ERK1/2, and K-ras protein in CRC were obtained, which were 66.3% （61/92）, 59.8% （55/92）, and 48.9% （45/92）, respectively, and those in normal epithelial cells of colon were 10% （2/20）, 20% （4/20）, and 30% （6/20）, respectively （P 〈 0.05）. The expressions of TGF-β1 and ERK1/2 in CRC at stage Ⅰwere 37.5% and 31.3%,respectively, and those in CRC at stage Ⅳ were 83.3% and79.3%, respectively, with statistically significant differences. No significant relationship was found between K-ras expression and tumor stages （P〉0.05）.CONCLUSION High level expressions of TGF-β1 and ERK1/2 are closely related to the clinical stages of colon cancer and crosstalk may exist between the 2 signal pathways.
文摘Charged amino acids (AAs) are targets for selective forces in protein evolution. To fully explore the trend of charged AA frequencies evolution in macroevolutionary process from prokaryotes to eukaryotes, we extend the analysis of five charged AAs separately and total basic and acidic AAs in protein sequences of 158 prokaryotic and 63 eukaryotic predicted proteomes and 456 clusters of orthologous groups (COGs). Also, we eliminate the biases that may caused by extreme organisms in both predicted proteomes and COGs analyses. More basic AAs, His,Lysand Glu were found in eukaryotic proteins compared with prokaryotic proteins by predicted proteomes analysis. By COGs analysis, we found that basic AAs andLysfrequencies are higher in eukaryotic orthologous proteins than their prokaryotic companions, while the trend of Arg frequency is the opposite. We discussed the agreements and disagreements of two analyses and gained a more credible trend of charged AAs evolution in macroevolutionary time scale.
文摘Objective: To investigate the effects of laparoscopic surgery and open surgery on serum tumor markers, coagulation function and immune function in patients with early endometrial cancer. Methods: The clinical data of 82 cases of early endometrial carcinoma were retrospectively analyzed, and they were divided into control group (n=40) and observation group (n=42) according to the treatment plan. Patients in the control group were treated with open surgery, and the observation group received laparoscopic treatment. The levels of serum tumor markers, coagulation function and immune function indexes of the two groups were compared between before operation and 1 d after operation. Results: The levels of serum CA-125, HE4, PT, APTT, FIB, CD3+, CD4+, CD8+ and CD4+/CD8+in the two groups before treatment were not significantly different. After treatment, the levels of CA-125, HE4, PT, APTT, CD3+, CD4+and CD4+/CD8+ in the two groups were significantly lower than those before treatment in the same group, and the levels of CA-125, HE4, PT and APTT in the observation group were significantly lower than those in the control group, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than those of the control group;After treatment, the levels of FIB in the two groups were significantly higher than that before treatment, and the level of observation group was significantly higher than that of the control group. Conclusion: Compared with open surgery for early endometrial cancer, laparoscopic surgery can effectively reduce the level of serum tumor markers and the inhibition of immune function, however, it has great influence on coagulation function, and effective measures should be taken to prevent the formation of thrombotic diseases.
基金All authors thank Zixin Han and Xinlin Li,from the Beijing University of Chinese Medicine,for providing methodological suggestions and polishing the manuscript.Our research was partly supported by TCM Science and technology development projects of Shandong Province of China(Grant No.2019-0075)Study of Preparation and Preliminary Pharmacodynamic of Zishen Anshen Oral Liquid(Grant No.2019-0076).
文摘Background:As a disease,insomnia is often ignored by the general public.Insomnia,which not directly fatal,may be equivalent to chronic suicide if it is not paid attention to.However,there are many theories on how to treat insomnia,and researchers have been searching for a cure.Chinese herbal medicine(CHM)and exercise therapy(ET)are relatively effective,and have been used since ancient times to treat insomnia.This study aimed to examine the effect of CHM combined with ET(CHM-ET)on patients with insomnia.Methods:We searched eight electronic databases including PubMed,Web of Science,EMBASE,the Cochrane Library,the China National Knowledge Infrastructure(CNKI),the China Biology Medicine disc(CBMdisc),the China Science and Technology Journal Database(VIP),the Wanfang Database to find randomized controlled trials(RCTs)evaluating CHM-ET in the treatment of insomnia patients up to September 13,2022.Two researchers read and screened the publications to extract data.We used the pittsburgh sleep quality index(PSQI)as the evaluation indicators for each study,and the other was the total effective rate(TER).The Cochrane risk-of-bias assessment tool was used to assess the risk of bias of the included literature.The level of evidence for this result was assessed by GARDE method.The meta-analysis was performed using STATA 14 software and RevMan 5.3.The research method was registered with PROSPERO(CRD42022350926).Results:We included fourteen randomized controlled trials,which including a total of 1,126 participants.Compared with the control group,the experimental group showed significantly improvements in sleep effect as reflected by the reduced PSQI score[mean difference(MD)=-2.47,95%confidence interval(CI)(-3.15,-1.78),I^(2)=92%]with low quality of evidence,and increased TER[risk ratio(RR)=1.23,95%CI(1.14,1.33),I^(2)=40%]with moderate quality of evidence.Compared with hypnotic drugs,CHM-ET significantly reduced the PSQI score[mean difference(MD)=-3.18,95%CI(-5.48,-0.89),I^(2)=73%]with low quality of evidence.The PSQI of CHM-ET significantly decreased compared with single CHM[mean difference(MD)=-3.04,95%CI(-5.84,-0.25),I^(2)=98%]with low quality of evidence,and ET[mean difference(MD)=-2.44,95%CI(-2.87,-2.02),I^(2)=0%]with moderate quality of evidence.No serious adverse reactions were observed.Conclusion:This review suggested that CHM-ET may be an effective treatment for insomnia.However,given the limited quality of the studies and methodologies included in the trials,further rigorous randomized controlled trials are needed for more accurate results.
基金Acknowledgments This research was supported by the following, Par-Eu Scholars Program, the National Natural Science Foundation of China （31372265, 31572332）, Coordinated Research Project of the International Atomic Energy Agency （18268/R0）, and National Key Program of Science and Technology Foundation Work of China （2015FY210300）. Conceived and designed the research： BC, XH. Performed the analysis： XH, BC, ZBH, YJZ, YZ, PJX, LQ. Wrote the paper： XH, BC, ZBH.
文摘Anopheles sinensis is a major malaria vector. Insect odorant-binding proteins （OBPs） may function in the reception of odorants in the olfactory system. The classification and characterization of the An. sinensis OBP genes have not been systematically studied. In this study, 64 putative OBP genes were identified at the whole-genome level of An. sinensis based on the comparison between OBP conserved motifs, PBP_GOBE and phylogenetic analysis with An. gambiae OBPs. The characterization of An. sinensis OBPs, including the motifs conservation, gene structure, genomic organization and classification, were investigated. A new gene, AsOBP73, belonging to the Plus-C subfamily, was identified with the support of transcript and conservative motifs. These An. sinensis OBP genes were classified into three subfamilies with 37, 15 and 12 genes in the subfamily Classic, Atypical and Plus-C, respectively. The genomic organization of An. sinensis OBPs suggests a clustered distribution across nine different scaffolds. Eight genes （0BP23-28, 0BP63- 64） might originate from a single gene through a series of historic duplication events at least before divergence of Anopheles, Culex and Aedes. The microsynteny analyses indicate a very high synteny between An. sinensis and An. gambiae OBPs. OBP70 and OBP71 earlier classified under Plus-C in An. gambiae are recognized as belonging to the group Obp59a of the Classic subfamily, and OBP69 earlier classified under Plus-C has been moved to the Atypical subfamily in this study. The study established a basic information frame for further study of the OBP genes in insects as well as in An. sinensis.
基金Acknowledgments This work was supported by Par-Eu Scholars Program, and The National Natural Science Foundation of China （31372265）, Coordinated Research Project of the International Atomic Energy Agency （18268/R0）, and National Key Program of Science and Technology Foun- dation Work of China （2015FY210300）. Conceived and designed the research： BC. Performed the experiments： FLS, DYE ZBH Analyzed the data and wrote the paper： YJH, BC, YJZ.
文摘The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause （SD）, high-throughput RNA-Seq data were generated from non-diapause （ND） and SD （initial, maintenance and quiescence phase） pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.
基金the National Natural Science Foundation of China(31672363,31372265)the Par-Eu Scholars Program(20136666)+2 种基金the Coordinated Research Project of the International Atomic En?ergy Agency(18268)the National Key Program of Science and Tech no logy Foundation Work of China(2015FY210300)and the Science and Technology Research Project of Chongqing Municipal Education Commission(KJ 1600304).
文摘Simple sequence repeats (SSRs) exist in both eukaryotic and prokaryotic genomes and are the most popular genetic markers, but the SSRs of mosquito genomes are still not well understood. In this study, we identified and analyzed the SSRs in 23 mosquito species using Drosophila melanogaster as reference at the whole-genome level. The results show that SSR numbers (33 076-560 175/genome) and genome sizes (574.57-1342.21 Mb) are significantly positively correlated (R~= 0.8992, P < 0.01), but the correlation in individual species varies in these mosquito species. In six types of SSR, mono- to trinucleotide SSRs are dominant with cumulative percentages of 95.14%-99.00% and densities of 195.65/Mb-787.51/Mb, whereas tetra- to hexanucleotide SSRs are rare with 1.12%-4.22% and 3.76/Mb-40.23/Mb. The (A/T)n,(AC/GT)n and (AGC/GCT)n are the most frequent motifs in mononucleotide, dinucleotide and trinucleotide SSRs, respectively, and the motif frequencies of tetra- to hexanucleotide SSRs appear to be species-specific. The 10-20 bp length of SSRs are dominant with the number of 11() 561 ± 93 482 and the frequency of 87.25%± 5.73% on average, and the number and frequency decline with the increase oflength. Most SSRs(83.34%± 7.72%) are located in intergenic regions, followed by intron regions (11.59%± 5.59%), exon regions (3.74%± 1.95%), and untranslated regions (1.32%± 1.39%). The mono-, di- and trinucleotide SSRs are the main SSRs in both gene regions (98.55%± 0.85%) and exon regions (99.27%± 0.52%). An average of 42.52% of total genes contains SSRs, and the preference for SSR occurrenee in different gene subcategories are species-specific. The study provides useful insights into the SSR diversity, characteristics and distribution in 23 mosquito species of genomes.
基金financially supported by the Aviation Science Fund(No.2017ZF25)the Graduate Research Innovation Fund of Yangzhou University(No.XKYCX19066)。
文摘Hydrogen nuclear magnetic resonance(H1-NMR) and X-ray diffractometer(XRD) were used to characterize the molecular and crystal structure of lead methacrylate [Pb(MAA)_(2)] which was produced by the double decomposition reaction of lead oxide and methacrylic acid.Isothermal analysis and Kelen-T(u|")dos(K-T) method were used to study the self-polymerization kinetic and the monomer reactivity ratios of Pb(M A A)_(2) and methyl methacrylate(MMA),respectively.By the ternary polymerization method of bulk casting using MMA,Pb(MAA)_(2) and gadolinium methacrylate(Gd(MAA)_(3)) as monomers,we prepared the plexiglass which have neutron and X-ray protection property.The results show that the polymerization rate(R_(p)) is expressed as Rp=K[M]^(1.02)[I]^(0.37) below the 10% conversion rate at 70℃,where K is the polymerization rate constant.And in the N,N-dimethylformamide(DMF) solution,the activation energy required for Pb(MAA)_(2) to initiate self-polymerization by the free radicals is 74.99 kJ·mol^(-1).The reactivity ratios of r1 [Pb(MAA)_(2)] and r2(MMA) are 3.767 and0.166.As the thickness of the material increases,the X-ray and thermal neutron shielding ability of the plexiglass containing gadolinium and lead is getting better and better.
文摘We investigate a continuous Heisenberg spin chain equation which models the local magnetization in ferromagnet with time-and site-dependent inhomogeneous bilinear interaction and timedependent spin-transfer torque.By establishing the gauge equivalence between the spin chain equation and an integrable generalized nonlinear Schrödinger equation,we present explicitly a novel nonautonomous magnetic soliton solution for the spin chain equation.The results display how the dynamics of the magnetic soliton can be controlled by the bilinear interaction and spin-polarized current.Especially,we find that the site-dependent bilinear interaction may break some conserved quantity,and give rise to damping-like effect in the spin evolution.
基金supported by the National Natural Science Foundation of China(Nos.31502033 and 31472164)the Fundamental Research Funds for the Central Universities(No.KJQN201615)+1 种基金the Natural Science Foundation of Jiangsu Province(No.BK20130688)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘Biological thiols（biothiols）, an important kind of functional biomolecules, such as cysteine（Cys） and glutathione（GSH）, play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.
基金financially supported by the Ministry of Science and Technology of China(973 Plan)(No2013CB632303)the National Natural Science Foundation of China(Nos.51001084 and 51275154)
文摘nc-Ti C/a-C：H nanocomposite films were prepared by filtered cathodic arc technique. The influence of C_2H_2/Ar flow ratio on the composition, structure, and mechanical properties of films was investigated by X-ray photoelectron spectroscopy（XPS）, X-ray diffraction（XRD）, Fourier transform infrared spectroscopy（FTIR）,Raman spectroscopy, nanoindentation, and ball-on-disc tribometry. The films show a nanocomposite structure in which Ti C crystallites are embedded in the amorphous matrix of a-C：H phase. C content in films increases with the flow ratio of C_2H_2/Ar, simultaneously, the crystallite size of TiC decreases. Contrary to the nc-Ti C/a-C：H films deposited by magnetron sputtering in which the sp3 C content increases with C_2H_2 flow rate, the increase of C_2H_2 flow rate leads to the increase of sp2 C content in films deposited by filtered cathodic arc technique. The nc-Ti C/a-C：H films deposited by cathodic arc technique have a pronounced hardness maximum of 30 GPa under the C_2H_2/Ar flow ratio of 12. Tribological performance of films is controlled by the sp2 content in films. Higher sp2 content promotes the formation of graphite-like transfer layer during sliding,and results in lower wear rate and friction coefficient.
