Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in...Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in GC and its potential mechanisms in regulating GC growth,and the relationship between BOP1 level in cancer tissues and survival was also analyzed.The expression of BOPI was examined by immunohistochemistry(IHC)in a cohort containing 387 patients with primary GC.Cultured GC cells were treated by siRNA to knock down the BOP1 expression,and examined by CCK-8 assay and plate clone formation to assess cell proliferation in vitro.Apoptotic rate of cultured GC cells was detected by flow cytometry with double staining of AnnxinV/PI.The xenografted mouse model was used to assess GC cell proliferation in vivo.Western blot and IHC were also performed to detect the expression levels of BOP1,p53 and p21.Patients with higher level of BOP1 in cancer tissues had significantly poorer survival.BOP1 silencing signifcantly suppressed GC cell proliferation both in vitro and in vivo.It blocked cell cycle at G0/G1 phase and led to apoptosis of GC cells via upregulating p53 and p21.BOP1 silencing-induced suppression of cell proliferation was partly reversed by pifithrin-a,(a p53 inhibitor).Our study dermonstrated that BOP1 up-regulation may be a hallmark of GC and it may regulate proliferation of GC cells by activating p53.BOP1 might be considered a novel biomarker of GC proliferation,and could be a potential indicator of prognosis of GC patients.BOP1 might also be a potential target for the treatment of GC patients if further researched.展开更多
As one class of the most important objects in the universe,magnetars can produce a lot of different frequency bursts including X-ray bursts.In Cai et al.,75 X-ray bursts produced by magnetar SGR J1935+2154 during an a...As one class of the most important objects in the universe,magnetars can produce a lot of different frequency bursts including X-ray bursts.In Cai et al.,75 X-ray bursts produced by magnetar SGR J1935+2154 during an active period in 2020 are published,including the duration and net photon counts of each burst,and waiting time based on the trigger time difference.In this paper,we utilize the power-law model,dN(x)/dx∝(x+x_0)~((-α)_x),to fit the cumulative distributions of these parameters.It can be found that all the cumulative distributions can be well fitted,which can be interpreted by a self-organizing criticality theory.Furthermore,we check whether this phenomenon still exists in different energy bands and find that there is no obvious evolution.These findings further confirm that the X-ray bursts from magnetars are likely to be generated by some self-organizing critical process,which can be explained by a possible magnetic reconnection scenario in magnetars.展开更多
Supergiant fast X-ray transients are a sub-class of high mass X-ray binaries,in which the compact object is a neutron star and it is accreting materials from its non-degenerate companion star.The sample of supergiant ...Supergiant fast X-ray transients are a sub-class of high mass X-ray binaries,in which the compact object is a neutron star and it is accreting materials from its non-degenerate companion star.The sample of supergiant fast X-ray transients from XMM-Newton public observations have been intensively studied,and hence the corresponding parameters for identifying X-ray flares have been obtained by Sidoli et al.,such as the rise time,the decay time,the duration time,the waiting time,the peak luminosity,the isotropic energy and the mean luminosity.In order to investigate the origin of the X-ray flares from the supergiant fast X-ray transients,we apply the cumulative distribution method with the power-law index dN(x)/dx∝(x+x0)-ax to the target sample.We then find that the relevant parameters can be well described by the cumulative distribution with indices 1.0-1.9 for IGR J16418-4532,IGR J16328-4726 and IGR J18450-0435,respectively.This finding indicates that the X-ray flares are likely to be produced by the self-organizing critical process,one possible scenario may be due to reconnection in magnetized stellar wind blobs approaching into the magnetosphere of magnetized neutron star.Therefore,we suggest that the high mass X-ray binaries with similar X-ray flares likely belong to the self-organized criticality systems.展开更多
Ultracompact dark matter minihalos （UCMHs） would be formed during the early universe if there were large density perturbations. If dark matter can decay into particles described by the standard model, such as neutri...Ultracompact dark matter minihalos （UCMHs） would be formed during the early universe if there were large density perturbations. If dark matter can decay into particles described by the standard model, such as neutrinos, these objects would become potential astrophysical sources of emission which could be detected by in- struments such as IceCube. In this paper, we investigate neutrino signals from nearby UCMHs due to gravitino dark matter decay and compare these signals with the back- ground neutrino flux which is mainly from the atmosphere to obtain constraints on the abundance of UCMHs.展开更多
Objective:In order to explore the neuroendocrine regulation mechanism of acupuncture and moxibustion in the treatment of allergic asthma and to provide theoretical basis for the effect of acupuncture and moxibustion o...Objective:In order to explore the neuroendocrine regulation mechanism of acupuncture and moxibustion in the treatment of allergic asthma and to provide theoretical basis for the effect of acupuncture and moxibustion on regulating mind.Methods:Forty SD rats were randomly divided into five groups:blank group,model group,sham-acupuncture group,acupuncture group and moxibustion group.Ovalbumin(OVA)was used to sensitise the rats.Except the blank group,the other groups were established by intraperitoneal injection of ovalbumin(OVA)and atomization excitation.The blank group was injected intraperitoneally and atomized with normal saline instead of OVA.After successful modeling,the rats in blank and the model group have no any treatment;In the sham acupuncture group,non-acupoint tissues were selected for acupuncture treatment every day;Acupuncture group and moxibustion group were treated with acupuncture or moxibustion at selected acupoints.At the end of the experiment,blood samples were collected to determine corticotropin releasing hormone(CRH).Results:After being sensitised by OVA,the symptoms scores of rats were significantly increased.After treatment,the symptoms of each treatment group were significantly reduced.Compared with model group,the CRH in acupuncture and moxibustion groups were significantly reduced(All P<0.01).Compared with the sham acupuncture group,the CRH in acupuncture group were significantly reduced(P<0.05).Conclusion:As a kind of stimulation,acupuncture and moxibustion can regulate the content of CRH in the body by nerve endocrine system.The effect of"regulating mind"of acupuncture and moxibustion plays an important role in the treatment of allergic asthma.展开更多
基金supported by National Natural Science Foundation of China(No.31670806)。
文摘Block of proliferation 1(BOP1)is a key protein involved in ribosome maturation and affects cancer progression.However,its role in gastric cancer(GC)remains unknown.This study aimed to explore the expression of BOPI in GC and its potential mechanisms in regulating GC growth,and the relationship between BOP1 level in cancer tissues and survival was also analyzed.The expression of BOPI was examined by immunohistochemistry(IHC)in a cohort containing 387 patients with primary GC.Cultured GC cells were treated by siRNA to knock down the BOP1 expression,and examined by CCK-8 assay and plate clone formation to assess cell proliferation in vitro.Apoptotic rate of cultured GC cells was detected by flow cytometry with double staining of AnnxinV/PI.The xenografted mouse model was used to assess GC cell proliferation in vivo.Western blot and IHC were also performed to detect the expression levels of BOP1,p53 and p21.Patients with higher level of BOP1 in cancer tissues had significantly poorer survival.BOP1 silencing signifcantly suppressed GC cell proliferation both in vitro and in vivo.It blocked cell cycle at G0/G1 phase and led to apoptosis of GC cells via upregulating p53 and p21.BOP1 silencing-induced suppression of cell proliferation was partly reversed by pifithrin-a,(a p53 inhibitor).Our study dermonstrated that BOP1 up-regulation may be a hallmark of GC and it may regulate proliferation of GC cells by activating p53.BOP1 might be considered a novel biomarker of GC proliferation,and could be a potential indicator of prognosis of GC patients.BOP1 might also be a potential target for the treatment of GC patients if further researched.
基金supported by the National Key R&D Program of China(2021YFA0718500)the National Natural Science Foundation of China under grants U2038106 and 12065017partially by the Jiangxi Provincial Natural Science Foundation under grant 20224ACB211001。
文摘As one class of the most important objects in the universe,magnetars can produce a lot of different frequency bursts including X-ray bursts.In Cai et al.,75 X-ray bursts produced by magnetar SGR J1935+2154 during an active period in 2020 are published,including the duration and net photon counts of each burst,and waiting time based on the trigger time difference.In this paper,we utilize the power-law model,dN(x)/dx∝(x+x_0)~((-α)_x),to fit the cumulative distributions of these parameters.It can be found that all the cumulative distributions can be well fitted,which can be interpreted by a self-organizing criticality theory.Furthermore,we check whether this phenomenon still exists in different energy bands and find that there is no obvious evolution.These findings further confirm that the X-ray bursts from magnetars are likely to be generated by some self-organizing critical process,which can be explained by a possible magnetic reconnection scenario in magnetars.
基金supported by the National Natural Science Foundation of China(grant No.U2038106)China Manned Spaced Project(CMS-CSST-2021-A12)。
文摘Supergiant fast X-ray transients are a sub-class of high mass X-ray binaries,in which the compact object is a neutron star and it is accreting materials from its non-degenerate companion star.The sample of supergiant fast X-ray transients from XMM-Newton public observations have been intensively studied,and hence the corresponding parameters for identifying X-ray flares have been obtained by Sidoli et al.,such as the rise time,the decay time,the duration time,the waiting time,the peak luminosity,the isotropic energy and the mean luminosity.In order to investigate the origin of the X-ray flares from the supergiant fast X-ray transients,we apply the cumulative distribution method with the power-law index dN(x)/dx∝(x+x0)-ax to the target sample.We then find that the relevant parameters can be well described by the cumulative distribution with indices 1.0-1.9 for IGR J16418-4532,IGR J16328-4726 and IGR J18450-0435,respectively.This finding indicates that the X-ray flares are likely to be produced by the self-organizing critical process,one possible scenario may be due to reconnection in magnetized stellar wind blobs approaching into the magnetosphere of magnetized neutron star.Therefore,we suggest that the high mass X-ray binaries with similar X-ray flares likely belong to the self-organized criticality systems.
基金Supported by the National Natural Science Foundation of China
文摘Ultracompact dark matter minihalos （UCMHs） would be formed during the early universe if there were large density perturbations. If dark matter can decay into particles described by the standard model, such as neutrinos, these objects would become potential astrophysical sources of emission which could be detected by in- struments such as IceCube. In this paper, we investigate neutrino signals from nearby UCMHs due to gravitino dark matter decay and compare these signals with the back- ground neutrino flux which is mainly from the atmosphere to obtain constraints on the abundance of UCMHs.
基金Science and Technology Innovation Training Project of Shanxi University of Traditional Chinese Medicine(2018py025).
文摘Objective:In order to explore the neuroendocrine regulation mechanism of acupuncture and moxibustion in the treatment of allergic asthma and to provide theoretical basis for the effect of acupuncture and moxibustion on regulating mind.Methods:Forty SD rats were randomly divided into five groups:blank group,model group,sham-acupuncture group,acupuncture group and moxibustion group.Ovalbumin(OVA)was used to sensitise the rats.Except the blank group,the other groups were established by intraperitoneal injection of ovalbumin(OVA)and atomization excitation.The blank group was injected intraperitoneally and atomized with normal saline instead of OVA.After successful modeling,the rats in blank and the model group have no any treatment;In the sham acupuncture group,non-acupoint tissues were selected for acupuncture treatment every day;Acupuncture group and moxibustion group were treated with acupuncture or moxibustion at selected acupoints.At the end of the experiment,blood samples were collected to determine corticotropin releasing hormone(CRH).Results:After being sensitised by OVA,the symptoms scores of rats were significantly increased.After treatment,the symptoms of each treatment group were significantly reduced.Compared with model group,the CRH in acupuncture and moxibustion groups were significantly reduced(All P<0.01).Compared with the sham acupuncture group,the CRH in acupuncture group were significantly reduced(P<0.05).Conclusion:As a kind of stimulation,acupuncture and moxibustion can regulate the content of CRH in the body by nerve endocrine system.The effect of"regulating mind"of acupuncture and moxibustion plays an important role in the treatment of allergic asthma.
