As a necessary factor for a successful business,entrepreneurial spirit is an important means for enterprises to survive in the unstable environment of fierce competition. Under the circumstance of mass entrepreneurshi...As a necessary factor for a successful business,entrepreneurial spirit is an important means for enterprises to survive in the unstable environment of fierce competition. Under the circumstance of mass entrepreneurship and innovation,a large number of small and medium-sized private enterprises have emerged in all walks of life in China. This paper examines the differences in the definition of entrepreneurial spirit by different scholars,the influence of Chinese entrepreneurship on the development of small and medium-sized private enterprises,the origin of the spirit of private entrepreneurs with Chinese characteristics,and the disadvantages of the spirit of small and medium-sized private entrepreneurs in China. It is necessary to further improve entrepreneurial spirit to contribute to the development of small and medium-sized private enterprises and help China to build the national economic system.展开更多
The efficacy of transarterial chemoembolization(TACE)has been limited by insufficient embolization and a high incidence of tumor recurrence.Herein,we iden-tified that aberrant metabolic reprogramming and immunosuppres...The efficacy of transarterial chemoembolization(TACE)has been limited by insufficient embolization and a high incidence of tumor recurrence.Herein,we iden-tified that aberrant metabolic reprogramming and immunosuppression contribute to TACE refractoriness and Rhein,as a potential glycolytic metabolism inhibitor and immunoactivation inducer,was optimized to sensitize tumors to TACE therapy.To achieve efficient embolization,we developed an oil-in-water lipiodol embolic emulsion by stabilizing the self-assembled Rhein nanogel.The assembled Rhein exhibited a nanofiber network,and its integration enhanced the mechanical stability and viscoelasticity of the lipiodol embolic agent.With the synergistic advantages of solid and liquid embolic agents,this carrier-free Pickering emulsion exhibits effi-cient embolization and sustained drug release in models of unilateral renal artery embolization,rabbit ear tumor embolization,rabbit orthotopic liver cancer,and rat orthotopic liver cancer.Compared to conventional three-way catheter mixing meth-ods,multimodal imaging corroborates a marked enhancement in local drug retention and tumor suppression.Importantly,the incorporation of Rhein-mediated syner-gistic immunoembolization in this strategy achieved efficient embolization while robustly activating anti-tumor immune responses,including inducing immunogenic cell death,dendritic cell activation,and major histocompatibility complex class I pre-sentation to CD8^(+)T cells for tumor killing.Together,thesefindings reveal a novel strategy for the application of self-assembled Rhein nanofiber-stabilized lipiodol emulsion to control metabolic signaling and immunoactivation in TACE.展开更多
Hepatocellular carcinoma(HCC)is globally the sixth most prevalent and the third most fatal malignancy[1].Although radical surgical resection is the optimal treat-ment strategy for HCC,it is not feasible for approximat...Hepatocellular carcinoma(HCC)is globally the sixth most prevalent and the third most fatal malignancy[1].Although radical surgical resection is the optimal treat-ment strategy for HCC,it is not feasible for approximately 80%of HCC patients who present at intermediate or advanced stages[2].Conversion therapy,particularly preoperative transcatheter arterial embolization/chemo-embolization(TAE/TACE),enables unresectable HCC patients to undergo radical resection;this transition from palliative care to curative surgery enhances overall prog-nosis of HCC patients[3].Many treatment guidelines recommend TACE as the first-line conversion therapy for patients with HCC[4];however,its success rate remains low(11.9%-24.0%)[5,6].Traditional manual and three-way tube mixing methods for preparing iodized oil and drug emulsions are limited by the relatively low drug-loading capacity and poor physical stability of the hydro-phobic iodized oil and hydrophilic drug formulations.As a result,the drugs are quickly released into circulation,leading to poor therapeutic effects and severe adverse events[7,8].The alternative method of drug-loaded microsphere embolization is costly and less effective due to microsphere size limitations[9].Therefore,finding ways to improve the success rate of conversion therapy is a major challenge that needs to be urgently addressed.展开更多
Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning.However,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycli...Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning.However,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycling of rhizosphere microbiomes remains unclear.To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres,we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies.Compared with the native mangrove(Kandelia obovata,KO),the introduced mangrove(Sonneratia apetala,SA)rhizosphere microbiome had significantly(p<0.05)higher average genome size(AGS)(5.8 vs.5.5 Mb),average 16S ribosomal RNA gene copy number(3.5 vs.3.1),relative abundances of mobile genetic elements,and functional diversity in terms of the Shannon index(7.88 vs.7.84)but lower functional potentials involved in CH4 cycling(e.g.,mcrABCDG and pmoABC),N2 fixation(nifHDK),and inorganic S cycling(dsrAB,dsrC,dsrMKJOP,soxB,sqr,and fccAB).Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere.Additionally,salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes.This study advances our understanding of microbially mediated biogeochemical cycling of CH_(4),N,and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions,which has important implications for future mangrove reforestation.展开更多
Mangroves are unique intertidal halophyte formations growing in sheltered tropical and subtropical coastal areas.Due to the increasing population and economic development,mangroves have faced degrada-tion and loss,whi...Mangroves are unique intertidal halophyte formations growing in sheltered tropical and subtropical coastal areas.Due to the increasing population and economic development,mangroves have faced degrada-tion and loss,which has been mainly caused by land conversion into aquaculture ponds in Asia.In the past several decades,the rapid growth of aquaculture has induced water pollution.Using mangroves for effluent treatment from coastal aquaculture ponds could be a suitable approach for wastewater treatment and healthy aquaculture development.An Integrated Mangrove-Aquaculture System(IMAS)was established to test whether the idea of a mangrove in situ treatment for aquaculture wastewater is feasible.The monocultures of three mangroves,Sonneratia caseolaris,Kandelia obo-vata,and Aegiceras corniculatum were established with area proportions of 45%,30%,or 15%,respectively.One control pond without mangroves was also set up.The results indicated that the mangroves had different toler-abilities to long-term inundation.The aquaculture ponds had different fishery yields,considering the mangrove species and area proportions.The water quality of most of the experimental ponds was better than the control pond,except for the planted Sonneratia.It is concluded that mangroves can reduce the concentration of dissolved inorganic nitrogen and phosphate,buffer the pH value and increase the concertration of dissolved oxygen in aqua-culture water bodies effectively.It is suggested to use 15%of the Aegiceras corniculatum area to conduct in situ purification of aquaculture wastewater and to enhance aquaculture production.展开更多
The most common treatment of spontaneous tumor rupture hemorrhage(STRH)is transcatheter arterial embolization(TAE)followed by liver resection,and surgical navigation using near-infrared fluorescence is effective metho...The most common treatment of spontaneous tumor rupture hemorrhage(STRH)is transcatheter arterial embolization(TAE)followed by liver resection,and surgical navigation using near-infrared fluorescence is effective method for detecting hidden lesions and ill-defined tumor boundaries.However,due to the blockage of the tumor-supplying artery after effective TAE treatment,it is difficult to deliver sufficient fluorescent probes to the tumor region.In this study,we report on the successful application of superstable homogeneous intermixed formulation technology(SHIFT)in precise conversion hepatectomy for ruptured hepatocellular carcinoma(HCC).A homogeneous lipiodol-ICG formulation obtained by SHIFT(SHIFT-ICG)was developed for clinical practice for STRH.A ruptured HCC patient received the combined protocol for embolization and fluorescence surgical navigation and exhibited excellent hemostatic effect.Lipiodol and ICG were both effectively deposited in the primary lesion,including a small metastatic lesion.In follow-up laparoscopic hepatectomy,SHIFT-ICG could clearly and precisely image the full tumor regions and boundaries in real time,and even indistinguishable satellite lesions still expressed a remarkable fluorescence intensity.In conclusion,the simple and green SHIFT-ICG formulation can be effectively used in emergency embolization hemostasis and later precise fluorescence navigation hepatectomy in patients with ruptured HCC bleeding and has high clinical application value.展开更多
基金Supported by Special Youth Project"A Comparative Study on the Cultivation Mode of Innovative Entrepreneurship Talents in China and South Korea"(120-1452226)
文摘As a necessary factor for a successful business,entrepreneurial spirit is an important means for enterprises to survive in the unstable environment of fierce competition. Under the circumstance of mass entrepreneurship and innovation,a large number of small and medium-sized private enterprises have emerged in all walks of life in China. This paper examines the differences in the definition of entrepreneurial spirit by different scholars,the influence of Chinese entrepreneurship on the development of small and medium-sized private enterprises,the origin of the spirit of private entrepreneurs with Chinese characteristics,and the disadvantages of the spirit of small and medium-sized private entrepreneurs in China. It is necessary to further improve entrepreneurial spirit to contribute to the development of small and medium-sized private enterprises and help China to build the national economic system.
基金Major State Basic Research Development Program of China,Grant/Award Number:2023YFB3810000National Natural Science Foundation of China,Grant/Award Numbers:U22A20333,81925019,U1705281,82202330+2 种基金Fundamental Research Funds for the Central Universities,Grant/Award Numbers:20720190088,20720200019Science Foundation of Fujian Province,Grant/Award Number:2020Y4003Program for New Century Excellent Talents in University,China,Grant/Award Number:NCET-13-0502。
文摘The efficacy of transarterial chemoembolization(TACE)has been limited by insufficient embolization and a high incidence of tumor recurrence.Herein,we iden-tified that aberrant metabolic reprogramming and immunosuppression contribute to TACE refractoriness and Rhein,as a potential glycolytic metabolism inhibitor and immunoactivation inducer,was optimized to sensitize tumors to TACE therapy.To achieve efficient embolization,we developed an oil-in-water lipiodol embolic emulsion by stabilizing the self-assembled Rhein nanogel.The assembled Rhein exhibited a nanofiber network,and its integration enhanced the mechanical stability and viscoelasticity of the lipiodol embolic agent.With the synergistic advantages of solid and liquid embolic agents,this carrier-free Pickering emulsion exhibits effi-cient embolization and sustained drug release in models of unilateral renal artery embolization,rabbit ear tumor embolization,rabbit orthotopic liver cancer,and rat orthotopic liver cancer.Compared to conventional three-way catheter mixing meth-ods,multimodal imaging corroborates a marked enhancement in local drug retention and tumor suppression.Importantly,the incorporation of Rhein-mediated syner-gistic immunoembolization in this strategy achieved efficient embolization while robustly activating anti-tumor immune responses,including inducing immunogenic cell death,dendritic cell activation,and major histocompatibility complex class I pre-sentation to CD8^(+)T cells for tumor killing.Together,thesefindings reveal a novel strategy for the application of self-assembled Rhein nanofiber-stabilized lipiodol emulsion to control metabolic signaling and immunoactivation in TACE.
基金National Natural Science Foundation of China,Grant/Award Numbers:81925019,U1705281,U22A20333,82202330Fundamental Research Funds for the Central Universities,Grant/Award Numbers:2020Y4003,20720200019Development Program of China,Grant/Award Number:2017YFA0205201。
文摘Hepatocellular carcinoma(HCC)is globally the sixth most prevalent and the third most fatal malignancy[1].Although radical surgical resection is the optimal treat-ment strategy for HCC,it is not feasible for approximately 80%of HCC patients who present at intermediate or advanced stages[2].Conversion therapy,particularly preoperative transcatheter arterial embolization/chemo-embolization(TAE/TACE),enables unresectable HCC patients to undergo radical resection;this transition from palliative care to curative surgery enhances overall prog-nosis of HCC patients[3].Many treatment guidelines recommend TACE as the first-line conversion therapy for patients with HCC[4];however,its success rate remains low(11.9%-24.0%)[5,6].Traditional manual and three-way tube mixing methods for preparing iodized oil and drug emulsions are limited by the relatively low drug-loading capacity and poor physical stability of the hydro-phobic iodized oil and hydrophilic drug formulations.As a result,the drugs are quickly released into circulation,leading to poor therapeutic effects and severe adverse events[7,8].The alternative method of drug-loaded microsphere embolization is costly and less effective due to microsphere size limitations[9].Therefore,finding ways to improve the success rate of conversion therapy is a major challenge that needs to be urgently addressed.
基金supported by the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(SML2020SP004)the National Natural Science Foundation of China(91951207,32100077,31770539,31870469,42177011,41676105,41771095,62170346)the China Postdoctoral Science Foundation(2021M703751).
文摘Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning.However,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycling of rhizosphere microbiomes remains unclear.To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres,we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies.Compared with the native mangrove(Kandelia obovata,KO),the introduced mangrove(Sonneratia apetala,SA)rhizosphere microbiome had significantly(p<0.05)higher average genome size(AGS)(5.8 vs.5.5 Mb),average 16S ribosomal RNA gene copy number(3.5 vs.3.1),relative abundances of mobile genetic elements,and functional diversity in terms of the Shannon index(7.88 vs.7.84)but lower functional potentials involved in CH4 cycling(e.g.,mcrABCDG and pmoABC),N2 fixation(nifHDK),and inorganic S cycling(dsrAB,dsrC,dsrMKJOP,soxB,sqr,and fccAB).Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere.Additionally,salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes.This study advances our understanding of microbially mediated biogeochemical cycling of CH_(4),N,and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions,which has important implications for future mangrove reforestation.
基金This research was supported by the“863 Project”of the Ministry of Science and Technology,China(No.2007AA091703 and 2003AA627030)。
文摘Mangroves are unique intertidal halophyte formations growing in sheltered tropical and subtropical coastal areas.Due to the increasing population and economic development,mangroves have faced degrada-tion and loss,which has been mainly caused by land conversion into aquaculture ponds in Asia.In the past several decades,the rapid growth of aquaculture has induced water pollution.Using mangroves for effluent treatment from coastal aquaculture ponds could be a suitable approach for wastewater treatment and healthy aquaculture development.An Integrated Mangrove-Aquaculture System(IMAS)was established to test whether the idea of a mangrove in situ treatment for aquaculture wastewater is feasible.The monocultures of three mangroves,Sonneratia caseolaris,Kandelia obo-vata,and Aegiceras corniculatum were established with area proportions of 45%,30%,or 15%,respectively.One control pond without mangroves was also set up.The results indicated that the mangroves had different toler-abilities to long-term inundation.The aquaculture ponds had different fishery yields,considering the mangrove species and area proportions.The water quality of most of the experimental ponds was better than the control pond,except for the planted Sonneratia.It is concluded that mangroves can reduce the concentration of dissolved inorganic nitrogen and phosphate,buffer the pH value and increase the concertration of dissolved oxygen in aqua-culture water bodies effectively.It is suggested to use 15%of the Aegiceras corniculatum area to conduct in situ purification of aquaculture wastewater and to enhance aquaculture production.
基金supported by the National Key R&D Program of China(2019YFA0110600).
文摘The most common treatment of spontaneous tumor rupture hemorrhage(STRH)is transcatheter arterial embolization(TAE)followed by liver resection,and surgical navigation using near-infrared fluorescence is effective method for detecting hidden lesions and ill-defined tumor boundaries.However,due to the blockage of the tumor-supplying artery after effective TAE treatment,it is difficult to deliver sufficient fluorescent probes to the tumor region.In this study,we report on the successful application of superstable homogeneous intermixed formulation technology(SHIFT)in precise conversion hepatectomy for ruptured hepatocellular carcinoma(HCC).A homogeneous lipiodol-ICG formulation obtained by SHIFT(SHIFT-ICG)was developed for clinical practice for STRH.A ruptured HCC patient received the combined protocol for embolization and fluorescence surgical navigation and exhibited excellent hemostatic effect.Lipiodol and ICG were both effectively deposited in the primary lesion,including a small metastatic lesion.In follow-up laparoscopic hepatectomy,SHIFT-ICG could clearly and precisely image the full tumor regions and boundaries in real time,and even indistinguishable satellite lesions still expressed a remarkable fluorescence intensity.In conclusion,the simple and green SHIFT-ICG formulation can be effectively used in emergency embolization hemostasis and later precise fluorescence navigation hepatectomy in patients with ruptured HCC bleeding and has high clinical application value.
