AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a grow...AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-XL in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-xu proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-XL were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of pStat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation.展开更多
AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between den...AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy.In this study, we fused human colon caner SW480 cells and human peripheral blood-derived DCs to induce an antitumor activity against human CC. METHODS:CC SW480 cells and human peripheral blood- derived DCs were fused with 500 mL/L polyethylene glycol (PEG). RESULTS:The specific T cell responses activated by fusion cells (FCs),were observed.About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases.In vitro studies showed that fusions effectively activated CD8^+ T lymphocytes to secrete interferon-γ.The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls,which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas. CONCLUSION:The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.展开更多
基金Supported by the National Natural Science Foundation of China,No.30271269
文摘AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-XL in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-xu proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-XL were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of pStat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation.
基金Supported by Technology Foundation of Ministry of Education,China
文摘AIM:The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients,especially those with micro- metastasis.The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy.In this study, we fused human colon caner SW480 cells and human peripheral blood-derived DCs to induce an antitumor activity against human CC. METHODS:CC SW480 cells and human peripheral blood- derived DCs were fused with 500 mL/L polyethylene glycol (PEG). RESULTS:The specific T cell responses activated by fusion cells (FCs),were observed.About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases.In vitro studies showed that fusions effectively activated CD8^+ T lymphocytes to secrete interferon-γ.The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls,which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas. CONCLUSION:The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.
