Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is unclear.The major purpose of this study was to investigate the mech...Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is unclear.The major purpose of this study was to investigate the mechanism of Lactiplantibacillus plantarun AR113 alleviating oxidative stress injury in the D-galactose induced aging mice.The result showed that pretreatment with L.plantarun AR113 significantly relieving H_(2)O_(2)induced cytotoxicity in HepG2 cells by maintain cell membrane integrity and increasing antioxidant enzyme activities.In D-galactose induced aging mice,L.plantarun AR113 could significantly attenuate liver damage and inflammatory infiltration by promoting endogenous glutathione(GSH)synthesis and activating the Nrf2/Keap1 signaling pathway in mice,and increasing the expression of regulated phaseⅡdetoxification enzymes and antioxidant enzymes.Further analysis shown that gavage of L.plantarun AR113 could significantly reduce the expression of G protein-coupled receptor 78(GPR78)and C/EBP homologous protein(CHOP)proteins,and promote the restoration of endoplasmic reticulum(ER)homeostasis,thereby activating cell anti-apoptotic pathways.These results were also confirmed in H_(2)O_(2)-treated HepG2 experiments.It indicated that L.plantarun AR113 could inhibit D-galactose-induced liver injury through dual inhibition of ER stress and oxidative stress.L.plantarun AR113 have good application potential in anti-aging and alleviating metabolic disorders.展开更多
Panax L.(Araliaceae) has a long history of medicinal and edible use due to its significant tonifying effects,and ginseng research has been a hot topic in natural products research and food science. In continuation of ...Panax L.(Araliaceae) has a long history of medicinal and edible use due to its significant tonifying effects,and ginseng research has been a hot topic in natural products research and food science. In continuation of our recent ginseng review, we highlighted the advances in ginseng research from 2021 to 2023 with 157 citations, which exhibited the increasingly systematic, collaborative, and intelligent characteristics.In this review, we firstly updated the progress in phytochemistry involving the ginsenosides and polysaccharides and summarized the researches on the active components. Then, some specific applications by feat of the multidimensional chromatography, mass spectrometry imaging, DNA barcoding, and metabolomics, were analyzed, which could provide rich information supporting the multi-component characterization, authentication, and quality control of ginseng and the versatile products. Finally, the recent biosynthesis studies concerning ginsenosides were retrospected. Additionally, the current challenges and future trends with respect to ginseng research were discussed.展开更多
Lactobacillus salivarius is an important probiotic lactic acid bacterium that plays a major role in innate and acquired immunity.It has gained much attention in metabolic engineering and synthetic biology owing to its...Lactobacillus salivarius is an important probiotic lactic acid bacterium that plays a major role in innate and acquired immunity.It has gained much attention in metabolic engineering and synthetic biology owing to its probiotic properties.However,few well-characterized constitutive promoters are available to modulate L.salivarius gene expression.Here,a pool of L.salivarius endogenous constitutive promoters was identified using RNA sequencing analysis.The promoters were sorted based on their transcript levels.The application potential of the promoters was evaluated using a fluorescence reporting system.This study provides the first well-characterized endogenous constitutive promoter library from L.salivarius,which has a potential for use as a basic toolbox in various lactic acid bacteria applications.展开更多
基金supported by the National Science Fund for Distinguished Young Scholars(32025029)the Shanghai Education Committee Scientific Research Innovation Projects(2101070007800120)+1 种基金the Yili Health Science Foundation of Chinese Institute of Food Science and Technology(2021-Y06)the Shanghai Engineering Research Center of food microbiology program(19DZ2281100)。
文摘Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is unclear.The major purpose of this study was to investigate the mechanism of Lactiplantibacillus plantarun AR113 alleviating oxidative stress injury in the D-galactose induced aging mice.The result showed that pretreatment with L.plantarun AR113 significantly relieving H_(2)O_(2)induced cytotoxicity in HepG2 cells by maintain cell membrane integrity and increasing antioxidant enzyme activities.In D-galactose induced aging mice,L.plantarun AR113 could significantly attenuate liver damage and inflammatory infiltration by promoting endogenous glutathione(GSH)synthesis and activating the Nrf2/Keap1 signaling pathway in mice,and increasing the expression of regulated phaseⅡdetoxification enzymes and antioxidant enzymes.Further analysis shown that gavage of L.plantarun AR113 could significantly reduce the expression of G protein-coupled receptor 78(GPR78)and C/EBP homologous protein(CHOP)proteins,and promote the restoration of endoplasmic reticulum(ER)homeostasis,thereby activating cell anti-apoptotic pathways.These results were also confirmed in H_(2)O_(2)-treated HepG2 experiments.It indicated that L.plantarun AR113 could inhibit D-galactose-induced liver injury through dual inhibition of ER stress and oxidative stress.L.plantarun AR113 have good application potential in anti-aging and alleviating metabolic disorders.
基金financially supported by National Key R&D Program of China (No. 2022YFC3501805)National Natural Science Foundation of China (No. 82374030)+3 种基金Tianjin Committee of Science and Technology of China (No. 23ZYJDSS00030)China Postdoctoral Science Foundation-Tianjin Joint Support Program (No. 2023T030TJ)Foundation of State Key Laboratory of Componentbased Chinese Medicine (No. CBQM2022103 and No. CBCM2023202)Tianjin Outstanding Youth Fund (No. 23JCJQJC00030)。
文摘Panax L.(Araliaceae) has a long history of medicinal and edible use due to its significant tonifying effects,and ginseng research has been a hot topic in natural products research and food science. In continuation of our recent ginseng review, we highlighted the advances in ginseng research from 2021 to 2023 with 157 citations, which exhibited the increasingly systematic, collaborative, and intelligent characteristics.In this review, we firstly updated the progress in phytochemistry involving the ginsenosides and polysaccharides and summarized the researches on the active components. Then, some specific applications by feat of the multidimensional chromatography, mass spectrometry imaging, DNA barcoding, and metabolomics, were analyzed, which could provide rich information supporting the multi-component characterization, authentication, and quality control of ginseng and the versatile products. Finally, the recent biosynthesis studies concerning ginsenosides were retrospected. Additionally, the current challenges and future trends with respect to ginseng research were discussed.
基金the Project of Shanghai for agriculture advance by science and technology,China(grant No.2022-02-08-00-12-F01102)Shanghai Education committee scientific research innovation projects,China(2101070007800120)+1 种基金Shandong Taishan Leading Talent Project(QY20201527)Major Scientific and Technological Innovation Project in Shandong Province(2019JZZY010341).
文摘Lactobacillus salivarius is an important probiotic lactic acid bacterium that plays a major role in innate and acquired immunity.It has gained much attention in metabolic engineering and synthetic biology owing to its probiotic properties.However,few well-characterized constitutive promoters are available to modulate L.salivarius gene expression.Here,a pool of L.salivarius endogenous constitutive promoters was identified using RNA sequencing analysis.The promoters were sorted based on their transcript levels.The application potential of the promoters was evaluated using a fluorescence reporting system.This study provides the first well-characterized endogenous constitutive promoter library from L.salivarius,which has a potential for use as a basic toolbox in various lactic acid bacteria applications.
