The interaction between HIV-1 DNA and five cyclic peptides （CP1-CP5） was investigated using electrospray ionization mass spectrometry （ESI-MS）. It revealed that CP1 [c（Ala-Tyr-Leu-Ala-Gly）] and CP4 [c（Pro-D-Tyr...The interaction between HIV-1 DNA and five cyclic peptides （CP1-CP5） was investigated using electrospray ionization mass spectrometry （ESI-MS）. It revealed that CP1 [c（Ala-Tyr-Leu-Ala-Gly）] and CP4 [c（Pro-D-Tyr-Leu-D-Ala-Gly）] have the higher binding affinity with the duplex DNA among the five cyclic peptides.展开更多
This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but different arrangements using electrospray mass spectrometry（ESI-MS）.A series of single-stranded ...This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but different arrangements using electrospray mass spectrometry（ESI-MS）.A series of single-stranded DNA were annealed with their complementary sequences,respectively.All the five pairs of hairpin DNA and coil DNA were unambiguously distinguished by ESIMS with annealing strategy.This research offers a potential method to probe the DNA structure by comparing with mass spectral characteristics.展开更多
基金Project supported by the Research Fund for the Doctoral Program of Higher Education.
文摘The interaction between HIV-1 DNA and five cyclic peptides （CP1-CP5） was investigated using electrospray ionization mass spectrometry （ESI-MS）. It revealed that CP1 [c（Ala-Tyr-Leu-Ala-Gly）] and CP4 [c（Pro-D-Tyr-Leu-D-Ala-Gly）] have the higher binding affinity with the duplex DNA among the five cyclic peptides.
基金Project supported by the National Natural Science Foundation of China(No.90913004) the 973 Program (No.2012CB720600)
文摘This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but different arrangements using electrospray mass spectrometry（ESI-MS）.A series of single-stranded DNA were annealed with their complementary sequences,respectively.All the five pairs of hairpin DNA and coil DNA were unambiguously distinguished by ESIMS with annealing strategy.This research offers a potential method to probe the DNA structure by comparing with mass spectral characteristics.
