Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improv...Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.展开更多
The objectives of the present work are focused on exploration of the reasons for temperature difference in different envelopes,the sensitivity of envelopes of solar greenhouse to thermal disfigurements and optimal sel...The objectives of the present work are focused on exploration of the reasons for temperature difference in different envelopes,the sensitivity of envelopes of solar greenhouse to thermal disfigurements and optimal selection of the thermal insulation quilt.Theoretical analysis and derivation are conducted according to the experimental results of surface temperature,convective heat transfer,and heat flux density in different envelopes which with thermal disfigurements of solar greenhouse using heat transfer theory.The results revealed that the difference of intrinsic thermal conductivity and thickness of the envelopes leads to difference in the thermal flux and thus in the surface temperature of different envelopes.Compared with the front roof and back roof,the wall is more sensitive to thermal disfigurements.According to the influence of thermal disfigurements on solar greenhouse temperature,the allowable thermal disfigurements area fraction of front roof and back roof are given.Based on the analysis of heat transfer theory and test results,the work given the expression of the thickness and thermal conductivity of thermal insulation quilt under different outdoor minimum temperatures for optimal selection of thermal insulation quilt based on comprehensive considering factors of the solar greenhouse structure,crop demand,outdoor minimum temperature,and insulation quilt properties.The results of this work give technical criterion for whether the envelopes with thermal disfigurements needs to be repaired or replaced,and it also provides theoretical and technical support for design the solar greenhouse as well as methodological guidance for the optimal selection of the thermal insulation quilt.展开更多
Bacteria and fungi are dominant members of environmental microbiomes.Various bacterial-fungal interactions(BFIs)and their mutual regulation are important factors for ecosystem functioning and health.Such interactions ...Bacteria and fungi are dominant members of environmental microbiomes.Various bacterial-fungal interactions(BFIs)and their mutual regulation are important factors for ecosystem functioning and health.Such interactions can be highly dynamic,and often require spatiotemporally resolved assessments to understand the interplay which ranges from antagonism to mutualism.Many of these interactions are still poorly understood,especially in terms of the underlying chemical and molecular interplay,which is crucial for inter-kingdom communication and interference.BFIs are highly relevant under agricultural settings;they can be determinative for crop health.Advancing our knowledge related to mechanisms underpinning the interactions between bacteria and fungi will provide an extended basis for biological control of pests and pathogens in agriculture.Moreover,it will facilitate a better understanding of complex microbial community networks that commonly occur in nature.This will allow us to determine factors that are crucial for community assembly under different environmental conditions and pave the way for constructing synthetic communities for various biotechnological applications.Here,we summarize the current advances in the field of BFIs with an emphasis on agriculture.展开更多
Crop production currently relies on the widespread use of agrochemicals to ensure food security.This practice is considered unsustainable,yet has no viable alternative at present.The plant microbiota can fulfil variou...Crop production currently relies on the widespread use of agrochemicals to ensure food security.This practice is considered unsustainable,yet has no viable alternative at present.The plant microbiota can fulfil various functions for its host,some of which could be the basis for developing sustainable protection and fertilization strategies for plants without relying on chemicals.To harness such functions,a detailed understanding of plant‒microbe and microbe‒microbe interactions is necessary.Among interactions within the plant microbiota,those between bacteria are the most common ones;they are not only of ecological importance but also essential for maintaining the health and productivity of the host plants.This review focuses on recent literature in this field and highlights various consequences of bacteria‒bacteria interactions under different agricultural settings.In addition,the molecular and genetic backgrounds of bacteria that facilitate such interactions are emphasized.Representative examples of commonly found bacterial metabolites with bioactive properties,as well as their modes of action,are given.Integrating our understanding of various binary interactions into complex models that encompass the entire microbiota will benefit future developments in agriculture and beyond,which could be further facilitated by artificial intelligence-based technologies.展开更多
基金This research was supported by the National Natural Science Foundation of China(32061143034,32161143028)Tibet Regional Science and Technology Collaborative Innovation Project(QYXTZX-NQ2021-01)Fundamental Research Funds for the Central Universities(lzujbky-2022-ct04).
文摘Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.
基金supported by Shaanxi Provincial Key Research and Development Program(Grant No.2019TSLNY01-03)National Natural Science Foundation of China(31901420)Young Scientist Promotion Project of Jiangsu Science and Technology Association(2020-2-46).
文摘The objectives of the present work are focused on exploration of the reasons for temperature difference in different envelopes,the sensitivity of envelopes of solar greenhouse to thermal disfigurements and optimal selection of the thermal insulation quilt.Theoretical analysis and derivation are conducted according to the experimental results of surface temperature,convective heat transfer,and heat flux density in different envelopes which with thermal disfigurements of solar greenhouse using heat transfer theory.The results revealed that the difference of intrinsic thermal conductivity and thickness of the envelopes leads to difference in the thermal flux and thus in the surface temperature of different envelopes.Compared with the front roof and back roof,the wall is more sensitive to thermal disfigurements.According to the influence of thermal disfigurements on solar greenhouse temperature,the allowable thermal disfigurements area fraction of front roof and back roof are given.Based on the analysis of heat transfer theory and test results,the work given the expression of the thickness and thermal conductivity of thermal insulation quilt under different outdoor minimum temperatures for optimal selection of thermal insulation quilt based on comprehensive considering factors of the solar greenhouse structure,crop demand,outdoor minimum temperature,and insulation quilt properties.The results of this work give technical criterion for whether the envelopes with thermal disfigurements needs to be repaired or replaced,and it also provides theoretical and technical support for design the solar greenhouse as well as methodological guidance for the optimal selection of the thermal insulation quilt.
基金supported by the National Natural Science Fund(31922074,32172356)the Key Technology R&D Program of Zhejiang Province(2019C02034)+1 种基金the China Agriculture Research System(CARS-3-29)the Fundamental Research Funds for the Central Universities(2021FZZX001–31).
文摘Bacteria and fungi are dominant members of environmental microbiomes.Various bacterial-fungal interactions(BFIs)and their mutual regulation are important factors for ecosystem functioning and health.Such interactions can be highly dynamic,and often require spatiotemporally resolved assessments to understand the interplay which ranges from antagonism to mutualism.Many of these interactions are still poorly understood,especially in terms of the underlying chemical and molecular interplay,which is crucial for inter-kingdom communication and interference.BFIs are highly relevant under agricultural settings;they can be determinative for crop health.Advancing our knowledge related to mechanisms underpinning the interactions between bacteria and fungi will provide an extended basis for biological control of pests and pathogens in agriculture.Moreover,it will facilitate a better understanding of complex microbial community networks that commonly occur in nature.This will allow us to determine factors that are crucial for community assembly under different environmental conditions and pave the way for constructing synthetic communities for various biotechnological applications.Here,we summarize the current advances in the field of BFIs with an emphasis on agriculture.
基金supported by the National Key Research and Development Program of China(No.2022YFD1400100)the Natural Science Foundation of Zhejiang Province(No.LZ23C140004)+1 种基金the National Natural Science Foundation of China(No.32172356)the China Agriculture Research System(No.CARS-3-1-15)。
文摘Crop production currently relies on the widespread use of agrochemicals to ensure food security.This practice is considered unsustainable,yet has no viable alternative at present.The plant microbiota can fulfil various functions for its host,some of which could be the basis for developing sustainable protection and fertilization strategies for plants without relying on chemicals.To harness such functions,a detailed understanding of plant‒microbe and microbe‒microbe interactions is necessary.Among interactions within the plant microbiota,those between bacteria are the most common ones;they are not only of ecological importance but also essential for maintaining the health and productivity of the host plants.This review focuses on recent literature in this field and highlights various consequences of bacteria‒bacteria interactions under different agricultural settings.In addition,the molecular and genetic backgrounds of bacteria that facilitate such interactions are emphasized.Representative examples of commonly found bacterial metabolites with bioactive properties,as well as their modes of action,are given.Integrating our understanding of various binary interactions into complex models that encompass the entire microbiota will benefit future developments in agriculture and beyond,which could be further facilitated by artificial intelligence-based technologies.
