Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yiel...Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows,indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis.Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B(Cyclin B), cell cycle protein D(Cyclin D), cell cycle protein E(Cyclin E), and cyclin-dependent kinase 4(CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and steroidogenic acute regulatory protein(StAR)(i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively.Conclusions: Our findings suggest that miR-214-3 p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development.展开更多
Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid a...Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid accumulation in bovine granulosa cells,which leads to a foam cell-like morphology and reduced steroidogenesis.However,why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear.This study focused on oleic acid’s effects on lipid type and steroidogenesis.Results:Oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level.Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro.In addition,oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2,but down-regulated the expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase,scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2,as well as steroidogenesis-related genes,including cytochrome P450 family 11 subfamily A member 1,cytochrome P450family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels.An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice,which resulted in lower estradiol levels than in control-fed mice.Compared with the control,decreases in estrus days and the numbers of antral follicles and corpora lutea,as well as an increase in the numbers of the atretic follicles,were found in the oleic acid-fed female mice.Conclusions:Oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells,and led to a decrease in steroidogenesis.These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.展开更多
Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis...Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), un-correlated linear discriminant analysis (ULDA), etc. Through the comparisons of the results, it can be found that ULDA and LDA combined with multiple scatter correction (MSC) pretreatment method successfully distinguish the patients of lung cancer and healthy people. The method has academic significance and promising clinical application value.展开更多
Phosphine(PH3)is an important factor driving the outbreak of cyanobacterial blooms that produce toxic microcystin threating human health.To clarify the physiological and biochemical responses of cyanobacteria to PH3 u...Phosphine(PH3)is an important factor driving the outbreak of cyanobacterial blooms that produce toxic microcystin threating human health.To clarify the physiological and biochemical responses of cyanobacteria to PH3 under elevated CO2 concentration,Microcystis aeruginosa was used in the coupling treatment of 1000 ppmv CO2 and PH3 at different concentrations respectively.The chlorophyll a(Chl-a),carotenoid,net photosynthetic rate and total protein of M.aeruginosa exhibited evidently increasing tendency under the coupling treatment of 1000 ppmv CO2 and PH 3 at different concentrations(7.51×10^−3,2.48×10^−2,7.51×10^−2 mg/L).The coupling treatments resulted in the higher concentrations of Chl-a and carotenoid of M.aeruginosa,compared to those in the control and the treatment with CO2 alone,and their enhancement increased with the increase in PH 3 concentrations.The total antioxidant capacity(T-AOC)in the coupling treatment with CO2 and PH3 of 2.48×10^−2 mg/L and 7.51×10^−3 mg/L showed increasing tendency,compared to the treatment with PH 3 alone.Additionally,the coupling treatment with 1000 ppmv CO2 and PH3 also altered the pH and DO level in the culture medium.In this regard,the coupling treatment with CO2 and PH3 at an appropriate concentration can enhance the resistance of M.aeruginosa to PH3 toxicity and is beneficial to the reproduction of M.aeruginosa,presumably resulting in potential for the outbreak of cyanobacteria bloom.Given the concern about global warming and the increase in atmospheric CO2 level,our research laid a foundation for the scientific understanding of the correlation between PH3 and cyanobacteria blooms.展开更多
Regulation of gas–solid flow is crucial for optimizing the operation efficiency of dual-circulating fluidized beds that are considered to be the most appropriate type of chemical-looping reactors. Herein, a computati...Regulation of gas–solid flow is crucial for optimizing the operation efficiency of dual-circulating fluidized beds that are considered to be the most appropriate type of chemical-looping reactors. Herein, a computational particle fluid dynamics method was employed to simulate the gas–solid flow in a 3-MWth dual-circulating fluidized bed used for chemical-looping combustion and gasification. The influence of structural difference between units on particle residence time was determined. The multi-parameter control mechanism of pressure, particle circulation, and particle residence time in a whole-loop system was investigated. Results revealed that under stable particle circulation, the particle residence time in the fuel reactor is much longer than that in the air reactor. The axial forces on the particles are reduced upon increasing particle density and size, leading to particle accumulation in the dense-phase zone. When the particle properties are stable, increasing the fluidizing gas flow rates by the same proportion leads to identical pressure drops on the involved two loop seals, which cause symmetrical alterations in the particle circulation rate between the air and fuel reactors. The dual-circulating fluidized bed exhibits certain multi-condition adaptability, which is limited by the stock bin volume. Overall, this study is beneficial for effective and economical optimization of the operation of chemical-looping dual-circulating fluidized beds.展开更多
基金supported by grants from the National Natural Science Foundation (No.31802047)the National Science and Technology Major Project of China (No. 2016ZX08006003)Shaanxi Provincial Key Research and Development Project (CN)(No. 2018ZDXM-NY-035)。
文摘Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows,indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis.Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B(Cyclin B), cell cycle protein D(Cyclin D), cell cycle protein E(Cyclin E), and cyclin-dependent kinase 4(CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and steroidogenic acute regulatory protein(StAR)(i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively.Conclusions: Our findings suggest that miR-214-3 p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development.
基金supported by grants from the National Natural Science Foundation(No.31802047)the National Science and Technology Major Project of China(No.2016ZX08006003)。
文摘Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid accumulation in bovine granulosa cells,which leads to a foam cell-like morphology and reduced steroidogenesis.However,why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear.This study focused on oleic acid’s effects on lipid type and steroidogenesis.Results:Oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level.Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro.In addition,oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2,but down-regulated the expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase,scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2,as well as steroidogenesis-related genes,including cytochrome P450 family 11 subfamily A member 1,cytochrome P450family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels.An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice,which resulted in lower estradiol levels than in control-fed mice.Compared with the control,decreases in estrus days and the numbers of antral follicles and corpora lutea,as well as an increase in the numbers of the atretic follicles,were found in the oleic acid-fed female mice.Conclusions:Oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells,and led to a decrease in steroidogenesis.These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.
文摘Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), un-correlated linear discriminant analysis (ULDA), etc. Through the comparisons of the results, it can be found that ULDA and LDA combined with multiple scatter correction (MSC) pretreatment method successfully distinguish the patients of lung cancer and healthy people. The method has academic significance and promising clinical application value.
基金This work was supported by the Natural Science Foundation of Guangdong Province(No.2017A030313239)Research Project of Sino-Singapore International Joint Research Institute(No.202-A018001)+4 种基金the Jiangsu Natural Science Foundation(Nos.BK20180109,BK)the State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences(No.2016SKL011)the Ministry of Science and Technology of China for State Key Research and Development Project(No.2016YFC04007082)the Student Research Project of South China University of Technology(No.7387)and the State Key Laboratory of Pollution Control and Resource Reuse(No.PCRRF14006,PCRRF17018).
文摘Phosphine(PH3)is an important factor driving the outbreak of cyanobacterial blooms that produce toxic microcystin threating human health.To clarify the physiological and biochemical responses of cyanobacteria to PH3 under elevated CO2 concentration,Microcystis aeruginosa was used in the coupling treatment of 1000 ppmv CO2 and PH3 at different concentrations respectively.The chlorophyll a(Chl-a),carotenoid,net photosynthetic rate and total protein of M.aeruginosa exhibited evidently increasing tendency under the coupling treatment of 1000 ppmv CO2 and PH 3 at different concentrations(7.51×10^−3,2.48×10^−2,7.51×10^−2 mg/L).The coupling treatments resulted in the higher concentrations of Chl-a and carotenoid of M.aeruginosa,compared to those in the control and the treatment with CO2 alone,and their enhancement increased with the increase in PH 3 concentrations.The total antioxidant capacity(T-AOC)in the coupling treatment with CO2 and PH3 of 2.48×10^−2 mg/L and 7.51×10^−3 mg/L showed increasing tendency,compared to the treatment with PH 3 alone.Additionally,the coupling treatment with 1000 ppmv CO2 and PH3 also altered the pH and DO level in the culture medium.In this regard,the coupling treatment with CO2 and PH3 at an appropriate concentration can enhance the resistance of M.aeruginosa to PH3 toxicity and is beneficial to the reproduction of M.aeruginosa,presumably resulting in potential for the outbreak of cyanobacteria bloom.Given the concern about global warming and the increase in atmospheric CO2 level,our research laid a foundation for the scientific understanding of the correlation between PH3 and cyanobacteria blooms.
基金supported by the key research and development program of Ningxia province of China(No.2018BCE01002)the national key research and development program of China(No.2018YFB0605401)+1 种基金the national natural science foundation of China(No.U20A20124)the innovation group project of Ningxia(No.2022AAC01001).
文摘Regulation of gas–solid flow is crucial for optimizing the operation efficiency of dual-circulating fluidized beds that are considered to be the most appropriate type of chemical-looping reactors. Herein, a computational particle fluid dynamics method was employed to simulate the gas–solid flow in a 3-MWth dual-circulating fluidized bed used for chemical-looping combustion and gasification. The influence of structural difference between units on particle residence time was determined. The multi-parameter control mechanism of pressure, particle circulation, and particle residence time in a whole-loop system was investigated. Results revealed that under stable particle circulation, the particle residence time in the fuel reactor is much longer than that in the air reactor. The axial forces on the particles are reduced upon increasing particle density and size, leading to particle accumulation in the dense-phase zone. When the particle properties are stable, increasing the fluidizing gas flow rates by the same proportion leads to identical pressure drops on the involved two loop seals, which cause symmetrical alterations in the particle circulation rate between the air and fuel reactors. The dual-circulating fluidized bed exhibits certain multi-condition adaptability, which is limited by the stock bin volume. Overall, this study is beneficial for effective and economical optimization of the operation of chemical-looping dual-circulating fluidized beds.
