Engineered photosynthetic bacterium Rhodo-pseudomonas palustris is excellent at one-step CO_(2) biomethanation and can use near-infrared light sources,overcoming the limitations of conventional photosynthetic systems....Engineered photosynthetic bacterium Rhodo-pseudomonas palustris is excellent at one-step CO_(2) biomethanation and can use near-infrared light sources,overcoming the limitations of conventional photosynthetic systems.The current study constructed a biohybrid system that deposited CdS nanoparticles on R.palustris.This biohybrid system broadens the capture of sustainable solar energy,achieving a 155 nmol-mL-biological CH,production under full visible light irradiation,13.4-fold of that by the pure R.palustris.The transcriptome profiles revealed that gene expression related to photosynthetic electron transfer chain,nitrogenase,nanofilaments,and redox stress defense was activated.Accordingly,we attributed the much-enhanced CO_(2) biomethanation in the biohybrid system to the remarkable increase in the intracellular reducing power and the stronger rigidity of the cells assisted by photoexcited electrons from CdS nanoparticles.Our discovery offers insight and a promising strategy forimproving the current CO_(2)-CH_(4) biomanufacturing system.展开更多
The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of n...The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of nanomaterials and the excellent selectivity of enzymes and microbes.This integration enables the conversion of solar energy into chemical products with high efficiency,attracting significant research interest from the fields of renewable energy and environmental science.Despite notable advances,the synergy mechanisms between abiotic nanomaterials and biotic enzymes/microbes remain unclear.This review outlines the latest progress in NBHS,encompassing material-enzyme hybrids and material-microbial hybrids,and explores design principles.Specifically,it examines the crucial role of electron transfer modes in enhancing the synergistic efficiency of nanomaterials and biological systems by analyzing various electron transfer mechanisms at the nanomaterial-biological interface.Drawing from existing literature,the review highlights the use of interfacial electron transfer mechanisms between coenzymes and cytochromes to elucidate nano/bio-material synergy.This fundamental understanding unveils opportunities to enhance biocompatible interfaces and electron transfer mechanisms,enabling non-photosensitive bacteria to harness solar energy for light-driven intracellular metabolism and CO_(2)bio-reduction into value-added chemicals.By offering a comprehensive overview of NBHS,this review also lays the groundwork for the development of more powerful systems aimed at achieving carbon neutrality.展开更多
Amyloid fibrils derived from different proteins have been proved as a promising material for adsorption of various pollutants from wastewater,which showed advantages of low cost and eco-friendliness.However,most of th...Amyloid fibrils derived from different proteins have been proved as a promising material for adsorption of various pollutants from wastewater,which showed advantages of low cost and eco-friendliness.However,most of the amyloid fibrils derived from animal-based proteins with high environmental footprint,while more sustainable amyloid fibrils derived from plant materials are desirable.In this study,a plant-derived amyloid fibril was extracted from the commonly used wheat flour with a simple and scalable protein purification and fibrillization process.Interestingly,the amyloid fibrils showed good adsorption capacity towards typical organic dyes(Eosin Y(EY)and Congo red(CR))from contaminated water.Adsorption kinetic analysis indicated the adsorption process to EY or CR by wheat flour amyloid well fitted with a pseudo-second-order model.The adsorption also followed a Langmuir isothermal model with adsorption capacities of 333 mg/g and 138 mg/g towards CR and EY,respectively.This work demonstrated the feasibility to utilize the plant-based amyloid fibril for organic dyes removal from contaminated water,which provided an affordable,sustainable and scalable tool for organic dyes removal from wastewater.展开更多
基金supported by the 2022 Carbon Dafeng and Carbon Neutral Science and Technology Innovation Special Fund in Jiangsu Province(Grant No.BK20220003)the National Natural Science Foundation of China(Grant No.32371538)+2 种基金the Special Funds for Jiangsu Provincial Science and technology plan(Grant No.BZ2022052)the Jiangsu Agriculture Science and Technology Innovation Fund(Grant No.CX(21)2015)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(Grant No.XTD2204).
文摘Engineered photosynthetic bacterium Rhodo-pseudomonas palustris is excellent at one-step CO_(2) biomethanation and can use near-infrared light sources,overcoming the limitations of conventional photosynthetic systems.The current study constructed a biohybrid system that deposited CdS nanoparticles on R.palustris.This biohybrid system broadens the capture of sustainable solar energy,achieving a 155 nmol-mL-biological CH,production under full visible light irradiation,13.4-fold of that by the pure R.palustris.The transcriptome profiles revealed that gene expression related to photosynthetic electron transfer chain,nitrogenase,nanofilaments,and redox stress defense was activated.Accordingly,we attributed the much-enhanced CO_(2) biomethanation in the biohybrid system to the remarkable increase in the intracellular reducing power and the stronger rigidity of the cells assisted by photoexcited electrons from CdS nanoparticles.Our discovery offers insight and a promising strategy forimproving the current CO_(2)-CH_(4) biomanufacturing system.
基金supported by the National Key Research and Development Program of China(2021YFA0910400)National Natural Science Foundation of China(21908083)+1 种基金Natural Science Foundation of Jiangsu Province(Carbon neutralization,BK20220003)Young Talents Cultivation Program of Jiangsu University.
文摘The nanomaterial-biological hybrid system(NBHS)is a rapidly growing interdisciplinary field that combines photocatalytic nanomaterials with biological systems,leveraging the superior light-harvesting capabilities of nanomaterials and the excellent selectivity of enzymes and microbes.This integration enables the conversion of solar energy into chemical products with high efficiency,attracting significant research interest from the fields of renewable energy and environmental science.Despite notable advances,the synergy mechanisms between abiotic nanomaterials and biotic enzymes/microbes remain unclear.This review outlines the latest progress in NBHS,encompassing material-enzyme hybrids and material-microbial hybrids,and explores design principles.Specifically,it examines the crucial role of electron transfer modes in enhancing the synergistic efficiency of nanomaterials and biological systems by analyzing various electron transfer mechanisms at the nanomaterial-biological interface.Drawing from existing literature,the review highlights the use of interfacial electron transfer mechanisms between coenzymes and cytochromes to elucidate nano/bio-material synergy.This fundamental understanding unveils opportunities to enhance biocompatible interfaces and electron transfer mechanisms,enabling non-photosensitive bacteria to harness solar energy for light-driven intracellular metabolism and CO_(2)bio-reduction into value-added chemicals.By offering a comprehensive overview of NBHS,this review also lays the groundwork for the development of more powerful systems aimed at achieving carbon neutrality.
基金Natural Science Foundation of Jiangsu Province(Carbon Neutralization,BK20220003)Special Scientific Research Project of School of Emergency Management,Jiangsu University(KY-B-02).
文摘Amyloid fibrils derived from different proteins have been proved as a promising material for adsorption of various pollutants from wastewater,which showed advantages of low cost and eco-friendliness.However,most of the amyloid fibrils derived from animal-based proteins with high environmental footprint,while more sustainable amyloid fibrils derived from plant materials are desirable.In this study,a plant-derived amyloid fibril was extracted from the commonly used wheat flour with a simple and scalable protein purification and fibrillization process.Interestingly,the amyloid fibrils showed good adsorption capacity towards typical organic dyes(Eosin Y(EY)and Congo red(CR))from contaminated water.Adsorption kinetic analysis indicated the adsorption process to EY or CR by wheat flour amyloid well fitted with a pseudo-second-order model.The adsorption also followed a Langmuir isothermal model with adsorption capacities of 333 mg/g and 138 mg/g towards CR and EY,respectively.This work demonstrated the feasibility to utilize the plant-based amyloid fibril for organic dyes removal from contaminated water,which provided an affordable,sustainable and scalable tool for organic dyes removal from wastewater.
