BACKGROUND Surgical resection is the primary treatment for hepatocellular carcinoma(HCC).However,studies indicate that nearly 70%of patients experience HCC recurrence within five years following hepatectomy.The earlie...BACKGROUND Surgical resection is the primary treatment for hepatocellular carcinoma(HCC).However,studies indicate that nearly 70%of patients experience HCC recurrence within five years following hepatectomy.The earlier the recurrence,the worse the prognosis.Current studies on postoperative recurrence primarily rely on postoperative pathology and patient clinical data,which are lagging.Hence,developing a new pre-operative prediction model for postoperative recurrence is crucial for guiding individualized treatment of HCC patients and enhancing their prognosis.AIM To identify key variables in pre-operative clinical and imaging data using machine learning algorithms to construct multiple risk prediction models for early postoperative recurrence of HCC.METHODS The demographic and clinical data of 371 HCC patients were collected for this retrospective study.These data were randomly divided into training and test sets at a ratio of 8:2.The training set was analyzed,and key feature variables with predictive value for early HCC recurrence were selected to construct six different machine learning prediction models.Each model was evaluated,and the bestperforming model was selected for interpreting the importance of each variable.Finally,an online calculator based on the model was generated for daily clinical practice.RESULTS Following machine learning analysis,eight key feature variables(age,intratumoral arteries,alpha-fetoprotein,preoperative blood glucose,number of tumors,glucose-to-lymphocyte ratio,liver cirrhosis,and pre-operative platelets)were selected to construct six different prediction models.The XGBoost model outperformed other models,with the area under the receiver operating characteristic curve in the training,validation,and test datasets being 0.993(95%confidence interval:0.982-1.000),0.734(0.601-0.867),and 0.706(0.585-0.827),respectively.Calibration curve and decision curve analysis indicated that the XGBoost model also had good predictive performance and clinical application value.CONCLUSION The XGBoost model exhibits superior performance and is a reliable tool for predicting early postoperative HCC recurrence.This model may guide surgical strategies and postoperative individualized medicine.展开更多
BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,fi...BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative.MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein.MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer,which promotes the exploration of the correlation between MRPL35 and GC.We proposed that the expression of MRPL35 might be critical in GC.AIM To study the effect of MRPL35 knockdown on GC cell proliferation.METHODS The expression of MRPL35 in GC was evaluated based on data from the publictumor database UALCAN(www.ualcan.path.uab.edu).The effect of theexpression of MRPL35 on the prognosis was evaluated with KMplot(www.kmplot.com).The expression of MRPL35 was assessed on the tissuemicroarray by immunohistochemistry and the level of MRPL35 mRNA in 25 pairsof clinical GC tissues and matched adjacent tissues was detected by quantitativereverse transcription-polymerase chain reaction.Celigo cell count assay,colonyformation assay,and flow cytometry were used to assess the role of MRPL35 inGC cell proliferation and apoptosis in vitro.Additionally,tumor formationexperiment in BALB/c nude mice was utilized to determine the effect of MRPL35on GC cell proliferation.After knockdown of MRPL35,related proteins wereidentified by isobaric tags for relative and absolute quantification analysis,andthe expression of related proteins was detected by Western blot.RESULTSThe expression of MRPL35 was up-regulated in GC(P=1.77×10^(-4)).The Kaplan-Meier plots of the overall survival indicated that high expression of MRPL35 wasassociated with a poor survival in GC.Compared with adjacent tissues,theexpression of MRPL35 in GC tissues was increased,which was related to age(P=0.03),lymph node metastasis(P=0.007),and pathological tumor-node-metastasisstage(P=0.024).Knockdown of MRPL35 inhibited GC cell proliferation andcolony formation and induced apoptosis.Animal experiment results showed thatknockdown of MRPL35 inhibited tumor formation in BALB/c nude mice.Westernblotting analysis showed that after knockdown of MRPL35,the expression ofPICK1 and BCL-XL proteins decreased,and that of AGR2 protein increased.CONCLUSIONCollectively,our findings demonstrate that knockdown of MRPL35 inhibits GCcell proliferation through related proteins including PICK1,BCL-XL,and AGR2.展开更多
BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evalua...BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.展开更多
Liver cancer is the sixth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide.Western medicine and therapies are the primary treatment strategies of hepatocellular carcinoma(HCC),but ...Liver cancer is the sixth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide.Western medicine and therapies are the primary treatment strategies of hepatocellular carcinoma(HCC),but the general prognosis for HCC patients is still dismal.Under these circumstances,HCC prevention is particularly important.Traditional Chinese medicine(TCM)encompasses a wealth of documented therapeutic resources,and“preventative treatment”is the principle of TCM.In China,TCM has been used for HCC prevention for thousands of years,and has also been demonstrated to be effective for the treatment of HCC in modern China.However,the TCM theory for prevention and treatment of HCC is more widely accepted in China than abroad.In this review,we first summarize the herbs and ancient formulas with therapeutic effects on HCC.We also review the research status of TCM in modern medicine as well as the current obstacles in its development.Finally,we discuss the future of TCM in the context of precision and integrated medicine.After reviewing the literature,we believe that TCM,through ancient development,is an advanced method of cancer treatment with positive curative effects,despite its surrounding controversy.Furthermore,precise analyses and systematic research methods provides novel approaches to modernize TCM for the future.展开更多
Background and Aims:This study aimed to determine the performance of the non-invasive score using noncontrastenhanced MRI(CHESS-DIS score)for detecting portal hy-pertension in cirrhosis.Methods:In this international m...Background and Aims:This study aimed to determine the performance of the non-invasive score using noncontrastenhanced MRI(CHESS-DIS score)for detecting portal hy-pertension in cirrhosis.Methods:In this international multicenter,diagnostic study(ClinicalTrials.gov,NCT03766880),patients with cirrhosis who had hepatic venous pressure gradient(HVPG)measurement and noncontrast-enhanced MRI were prospectively recruited from four university hospitals in China(n=4)and Turkey(n=1)between December 2018 and April 2019.A cohort of patients was retrospectively recruited from a university hospital in Italy between March 2015 and November 2017.After segmentation of the liver on fat-suppressed T1-weighted MRI maps,CHESS-DIS score was calculated automatically by an in-house developed code based on the quantification of liver surface nodularity.Results:A total of 149 patients were included,of which 124 were from four Chinese hospitals(training cohort)and 25 were from two international hospitals(validation cohort).A positive correlation between CHESS-DIS score and HVPG was found with the correlation coefficients of 0.36(p<0.0001)and 0.55(p<0.01)for the training and validation cohorts,respectively.The area under the receiver operating characteristic curve of CHESS-DIS score in detection of clinically significant portal hypertension(CSPH)was 0.81 and 0.9 in the training and validation cohorts,respectively.The intra-class correlation coefficients for assessing the inter-and intra-observer agreement were 0.846 and 0.841,respectively.Conclusions:A non-invasive score using noncontrast-enhanced MRI was developed and proved to be significantly correlated with invasive HVPG.Besides,this score could be used to detect CSPH in patients with cirrhosis.展开更多
Precise measurement of mechanical properties of living cells is important in understanding their mechanics-biology relations.In this study,we adopted the atomic force microscope to measure the creep deformation and st...Precise measurement of mechanical properties of living cells is important in understanding their mechanics-biology relations.In this study,we adopted the atomic force microscope to measure the creep deformation and stress relaxation of six different human cell lines.We examined whether the measured creep and relaxation trajectories satisfy a verification relation derived based on the linear viscoelastic theory.We compared the traditional spring-dashpot and the newly developed power-law-type constitutive relations in fitting the experimental measurements.We found that the human normal liver(L02),hepatic cancer(HepG2),hepatic stellate(LX2)and gastric cancer(NCI-N87)cell lines are linear viscoelastic materials,and human normal gastric(GES-1)and gastric cancer(SGC7901)cell lines are nonlinear due to failing in satisfying the verification relation for linear viscoelastic theory.The three-parameter power-law-type const计utive relation can fit the experimentai measurements better than that of the five-parameter classical spring-dashpot.展开更多
Aims:Splenectomy combined with pericardial devascularization is one of the important methods to treat hypersplenism,gastrointestinal bleeding,and other complications caused by liver cirrhosis;however,it is accompanied...Aims:Splenectomy combined with pericardial devascularization is one of the important methods to treat hypersplenism,gastrointestinal bleeding,and other complications caused by liver cirrhosis;however,it is accompanied by a high risk of portal vein thrombosis(PVT).This study aimed to explore the preventive and therapeutic effects of proximal splenic vein ligation(PSVL)with postoperative transcatheter anticoagulant therapy(TCAT)on PVT.Methods:This study retrospectively selected 143 patients with liver cirrhosis and portal hypertension,who received splenectomy combined with pericardial devascularization from June 30,2018 to June 30,2021.According to computed tomography photography,within 1 week before the operation,the patients were divided into a prevention group(without preoperative PVT,n=112)and a treatment group(preoperative PVT,n=31).Then,each group was subdivided based on the treatment and prevention measures into PSVL+TCAT(n=70)and systemic anticoagulant therapy(SAT)subgroups(n=73).The preventive and therapeutic effects of PSVL followed by TCAT on PVT were analyzed.Results:The operation time in the PSVL+TCAT subgroups was longer than that in the SAT subgroups(185±76 min vs.161±55 min;p<0.01).There was no difference between the two subgroups in terms of operative bleeding(345±82 mL vs.336±65 mL;p>0.50).There was no operative death,and all patients recovered uneventfully.In the prevention group,procedure-related complications occurred in two patients in the PSVL+TCAT subgroup(3.7%[2/54]),including one patient with slight pancreatitis and one patient with chylous leakage,owing to mobilization of the pancreas.The PVT incidence in the prevention group was significantly different between the two subgroups at postoperative 7th day,3rd month,and 6th month(PSVL+TCAT:0%,11.1%,and 5.6%vs.SAT:39.7%,31.0%,and 20.7%,respectively;all p<0.05).In the treatment group,the thrombus regression rate at postoperative 7th day and disappearance rates at the 3rd month and the 6th month of the PSVL+TCAT subgroup were significantly higher than those in the SAT subgroup after anticoagulant and thrombolysis therapy(PSVL+TCAT:75.0%,68.8%,and 87.5%vs.SAT:20.0%,26.7%,and 40.0%;all p<0.05).Conclusions:PSVL+TCAT reduces the risk of PVT after splenectomy and is safe and effective in treating PVT during surgery for portal hypertension.展开更多
基金Supported by Ningxia Key Research and Development Program,No.2018BEG03001.
文摘BACKGROUND Surgical resection is the primary treatment for hepatocellular carcinoma(HCC).However,studies indicate that nearly 70%of patients experience HCC recurrence within five years following hepatectomy.The earlier the recurrence,the worse the prognosis.Current studies on postoperative recurrence primarily rely on postoperative pathology and patient clinical data,which are lagging.Hence,developing a new pre-operative prediction model for postoperative recurrence is crucial for guiding individualized treatment of HCC patients and enhancing their prognosis.AIM To identify key variables in pre-operative clinical and imaging data using machine learning algorithms to construct multiple risk prediction models for early postoperative recurrence of HCC.METHODS The demographic and clinical data of 371 HCC patients were collected for this retrospective study.These data were randomly divided into training and test sets at a ratio of 8:2.The training set was analyzed,and key feature variables with predictive value for early HCC recurrence were selected to construct six different machine learning prediction models.Each model was evaluated,and the bestperforming model was selected for interpreting the importance of each variable.Finally,an online calculator based on the model was generated for daily clinical practice.RESULTS Following machine learning analysis,eight key feature variables(age,intratumoral arteries,alpha-fetoprotein,preoperative blood glucose,number of tumors,glucose-to-lymphocyte ratio,liver cirrhosis,and pre-operative platelets)were selected to construct six different prediction models.The XGBoost model outperformed other models,with the area under the receiver operating characteristic curve in the training,validation,and test datasets being 0.993(95%confidence interval:0.982-1.000),0.734(0.601-0.867),and 0.706(0.585-0.827),respectively.Calibration curve and decision curve analysis indicated that the XGBoost model also had good predictive performance and clinical application value.CONCLUSION The XGBoost model exhibits superior performance and is a reliable tool for predicting early postoperative HCC recurrence.This model may guide surgical strategies and postoperative individualized medicine.
文摘BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative.MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein.MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer,which promotes the exploration of the correlation between MRPL35 and GC.We proposed that the expression of MRPL35 might be critical in GC.AIM To study the effect of MRPL35 knockdown on GC cell proliferation.METHODS The expression of MRPL35 in GC was evaluated based on data from the publictumor database UALCAN(www.ualcan.path.uab.edu).The effect of theexpression of MRPL35 on the prognosis was evaluated with KMplot(www.kmplot.com).The expression of MRPL35 was assessed on the tissuemicroarray by immunohistochemistry and the level of MRPL35 mRNA in 25 pairsof clinical GC tissues and matched adjacent tissues was detected by quantitativereverse transcription-polymerase chain reaction.Celigo cell count assay,colonyformation assay,and flow cytometry were used to assess the role of MRPL35 inGC cell proliferation and apoptosis in vitro.Additionally,tumor formationexperiment in BALB/c nude mice was utilized to determine the effect of MRPL35on GC cell proliferation.After knockdown of MRPL35,related proteins wereidentified by isobaric tags for relative and absolute quantification analysis,andthe expression of related proteins was detected by Western blot.RESULTSThe expression of MRPL35 was up-regulated in GC(P=1.77×10^(-4)).The Kaplan-Meier plots of the overall survival indicated that high expression of MRPL35 wasassociated with a poor survival in GC.Compared with adjacent tissues,theexpression of MRPL35 in GC tissues was increased,which was related to age(P=0.03),lymph node metastasis(P=0.007),and pathological tumor-node-metastasisstage(P=0.024).Knockdown of MRPL35 inhibited GC cell proliferation andcolony formation and induced apoptosis.Animal experiment results showed thatknockdown of MRPL35 inhibited tumor formation in BALB/c nude mice.Westernblotting analysis showed that after knockdown of MRPL35,the expression ofPICK1 and BCL-XL proteins decreased,and that of AGR2 protein increased.CONCLUSIONCollectively,our findings demonstrate that knockdown of MRPL35 inhibits GCcell proliferation through related proteins including PICK1,BCL-XL,and AGR2.
基金This study is registered at https://www.clinicaltrials.gov/.The registration identification number is NCT04639323.
文摘BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.
基金This research project was mainly supported by the National Natural Science Foundation of China(81502694)This research project was also partly supported by the Fundamental Research Funds for the Central Universities(1191329835)+1 种基金Postdoctoral Science Foundation of China(2015M570330)Key Projects of Ningxia Natural Science Foundation(NZ15130).
文摘Liver cancer is the sixth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide.Western medicine and therapies are the primary treatment strategies of hepatocellular carcinoma(HCC),but the general prognosis for HCC patients is still dismal.Under these circumstances,HCC prevention is particularly important.Traditional Chinese medicine(TCM)encompasses a wealth of documented therapeutic resources,and“preventative treatment”is the principle of TCM.In China,TCM has been used for HCC prevention for thousands of years,and has also been demonstrated to be effective for the treatment of HCC in modern China.However,the TCM theory for prevention and treatment of HCC is more widely accepted in China than abroad.In this review,we first summarize the herbs and ancient formulas with therapeutic effects on HCC.We also review the research status of TCM in modern medicine as well as the current obstacles in its development.Finally,we discuss the future of TCM in the context of precision and integrated medicine.After reviewing the literature,we believe that TCM,through ancient development,is an advanced method of cancer treatment with positive curative effects,despite its surrounding controversy.Furthermore,precise analyses and systematic research methods provides novel approaches to modernize TCM for the future.
基金the National Natural Science Foundation of China(81830053,82001780)Guangzhou Industry-Academia-Research Collaborative Innovation Major Project(201704020015)+2 种基金Natural Science Foundation of Jiangsu Province of China(BK20200361)President Foundation of Nanfang Hospital,Southern Medical University(2017Z012)Distinguished Young Scholars of Gansu Province(20JR10RA713).
文摘Background and Aims:This study aimed to determine the performance of the non-invasive score using noncontrastenhanced MRI(CHESS-DIS score)for detecting portal hy-pertension in cirrhosis.Methods:In this international multicenter,diagnostic study(ClinicalTrials.gov,NCT03766880),patients with cirrhosis who had hepatic venous pressure gradient(HVPG)measurement and noncontrast-enhanced MRI were prospectively recruited from four university hospitals in China(n=4)and Turkey(n=1)between December 2018 and April 2019.A cohort of patients was retrospectively recruited from a university hospital in Italy between March 2015 and November 2017.After segmentation of the liver on fat-suppressed T1-weighted MRI maps,CHESS-DIS score was calculated automatically by an in-house developed code based on the quantification of liver surface nodularity.Results:A total of 149 patients were included,of which 124 were from four Chinese hospitals(training cohort)and 25 were from two international hospitals(validation cohort).A positive correlation between CHESS-DIS score and HVPG was found with the correlation coefficients of 0.36(p<0.0001)and 0.55(p<0.01)for the training and validation cohorts,respectively.The area under the receiver operating characteristic curve of CHESS-DIS score in detection of clinically significant portal hypertension(CSPH)was 0.81 and 0.9 in the training and validation cohorts,respectively.The intra-class correlation coefficients for assessing the inter-and intra-observer agreement were 0.846 and 0.841,respectively.Conclusions:A non-invasive score using noncontrast-enhanced MRI was developed and proved to be significantly correlated with invasive HVPG.Besides,this score could be used to detect CSPH in patients with cirrhosis.
基金We acknowledge the Institute of Pathology,School of Basic Medical Sciences,Lanzhou University,for providing cells.This study is supported by grants from the National Natural Science Foundation of China(11472119,11602099)the 111 Project(B14044).
文摘Precise measurement of mechanical properties of living cells is important in understanding their mechanics-biology relations.In this study,we adopted the atomic force microscope to measure the creep deformation and stress relaxation of six different human cell lines.We examined whether the measured creep and relaxation trajectories satisfy a verification relation derived based on the linear viscoelastic theory.We compared the traditional spring-dashpot and the newly developed power-law-type constitutive relations in fitting the experimental measurements.We found that the human normal liver(L02),hepatic cancer(HepG2),hepatic stellate(LX2)and gastric cancer(NCI-N87)cell lines are linear viscoelastic materials,and human normal gastric(GES-1)and gastric cancer(SGC7901)cell lines are nonlinear due to failing in satisfying the verification relation for linear viscoelastic theory.The three-parameter power-law-type const计utive relation can fit the experimentai measurements better than that of the five-parameter classical spring-dashpot.
基金funded by the Major Research&Development Program of the Ningxia Hui Autonomous Region (2022BEG02038)the Technology Benefit Project of the Ningxia Hui Autonomous Region (2021CMG03017).
文摘Aims:Splenectomy combined with pericardial devascularization is one of the important methods to treat hypersplenism,gastrointestinal bleeding,and other complications caused by liver cirrhosis;however,it is accompanied by a high risk of portal vein thrombosis(PVT).This study aimed to explore the preventive and therapeutic effects of proximal splenic vein ligation(PSVL)with postoperative transcatheter anticoagulant therapy(TCAT)on PVT.Methods:This study retrospectively selected 143 patients with liver cirrhosis and portal hypertension,who received splenectomy combined with pericardial devascularization from June 30,2018 to June 30,2021.According to computed tomography photography,within 1 week before the operation,the patients were divided into a prevention group(without preoperative PVT,n=112)and a treatment group(preoperative PVT,n=31).Then,each group was subdivided based on the treatment and prevention measures into PSVL+TCAT(n=70)and systemic anticoagulant therapy(SAT)subgroups(n=73).The preventive and therapeutic effects of PSVL followed by TCAT on PVT were analyzed.Results:The operation time in the PSVL+TCAT subgroups was longer than that in the SAT subgroups(185±76 min vs.161±55 min;p<0.01).There was no difference between the two subgroups in terms of operative bleeding(345±82 mL vs.336±65 mL;p>0.50).There was no operative death,and all patients recovered uneventfully.In the prevention group,procedure-related complications occurred in two patients in the PSVL+TCAT subgroup(3.7%[2/54]),including one patient with slight pancreatitis and one patient with chylous leakage,owing to mobilization of the pancreas.The PVT incidence in the prevention group was significantly different between the two subgroups at postoperative 7th day,3rd month,and 6th month(PSVL+TCAT:0%,11.1%,and 5.6%vs.SAT:39.7%,31.0%,and 20.7%,respectively;all p<0.05).In the treatment group,the thrombus regression rate at postoperative 7th day and disappearance rates at the 3rd month and the 6th month of the PSVL+TCAT subgroup were significantly higher than those in the SAT subgroup after anticoagulant and thrombolysis therapy(PSVL+TCAT:75.0%,68.8%,and 87.5%vs.SAT:20.0%,26.7%,and 40.0%;all p<0.05).Conclusions:PSVL+TCAT reduces the risk of PVT after splenectomy and is safe and effective in treating PVT during surgery for portal hypertension.
