Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for cancer diagnosis in liquid biopsy.Because of their nanoscale size,complex biogenesis,and met...Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for cancer diagnosis in liquid biopsy.Because of their nanoscale size,complex biogenesis,and methodological limitations related to exosome isolation and detection,advancements in their analysis remain slow.Microfluidic technology offers a better analytic approach compared with conventional methods.Here,we developed a bead-based microarray for exosome isolation and multiplexed tumor marker detection.Using this method,exosomes are isolated by binding to antibodies on the bead surface,and tumor markers on the exosomes are detected through quantum dot(QD)probes.The beads are then uniformly trapped and queued among micropillars in the chip.This design benefits fluorescence observation by dispersing the signals into every single bead,thereby avoiding optical interference and enabling more accurate test results.We analyzed exosomes in the cell culture supernatant of lung cancer and endothelial cell lines,and different lung cancer markers labeled with three QD probes were used to conduct multiplexed detection of exosome surface protein markers.Lung cancer-derived samples showed much higher(~sixfold-tenfold)fluorescence intensity than endothelial cell samples,and different types of lung cancer samples showed distinctive marker expression levels.Additionally,using the chip to detect clinical plasma samples from cancer patients showed good diagnostic power and revealed a well consistency with conventional tests for serological markers.These results provide insight into a promising method for exosome tumor marker detection and early-stage cancer diagnosis.展开更多
Existing multi-view deep subspace clustering methods aim to learn a unified representation from multi-view data,while the learned representation is difficult to maintain the underlying structure hidden in the origin s...Existing multi-view deep subspace clustering methods aim to learn a unified representation from multi-view data,while the learned representation is difficult to maintain the underlying structure hidden in the origin samples,especially the high-order neighbor relationship between samples.To overcome the above challenges,this paper proposes a novel multi-order neighborhood fusion based multi-view deep subspace clustering model.We creatively integrate the multi-order proximity graph structures of different views into the self-expressive layer by a multi-order neighborhood fusion module.By this design,the multi-order Laplacian matrix supervises the learning of the view-consistent self-representation affinity matrix;then,we can obtain an optimal global affinity matrix where each connected node belongs to one cluster.In addition,the discriminative constraint between views is designed to further improve the clustering performance.A range of experiments on six public datasets demonstrates that the method performs better than other advanced multi-view clustering methods.The code is available at https://github.com/songzuolong/MNF-MDSC(accessed on 25 December 2024).展开更多
Modified starch was better suitable for food processing,and fermentation was one of the effective methods to modify starch.This study investigated the separate and synergistic fermentation of Lactiplantibacillus plant...Modified starch was better suitable for food processing,and fermentation was one of the effective methods to modify starch.This study investigated the separate and synergistic fermentation of Lactiplantibacillus plantarum and Saccharomyces cerevisiae could whether affect the multi-scale structure and physicochemical properties of highland barley starch(HBS).The results of multi-scale structure determination of HBS showed that compared with unfermented HBS(32.27%),fermentation showed a significant increase in relative crystallinity(35.49-39.81%),while the crystal type of HBS as observed from X-ray diffraction(A-type crystalline pattern)did not change.The small angle X-ray scattering revealed that fermentation reduced the amorphous region and increased the crystalline layer thickness of the HBS.Consequently,the changes in the physicochemical properties showed that the peak viscosity,trough viscosity,and final viscosity of HBS were reduced after fermentation,and the aging of starch gel was delayed significantly.Our results showed that Lactiplantibacillus plantarum and Saccharomyces cerevisiae fermentations improve the physicochemical properties of HBS by modifying the multi-scale structure of starch,especially the synergistic fermentation effect was more effective.展开更多
In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle （AuNP） probes and a gene chip to detect microRNAs （miRNAs）. Specific oligonucleotides were attached to...In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle （AuNP） probes and a gene chip to detect microRNAs （miRNAs）. Specific oligonucleotides were attached to the glass surface as capture probes for the target miRNAs, which were then detected via hybridization to the AuNP probes. The signal was amplified via the re- duction of HAuCI4 by H202. The use of a single AuNP probe detected 10 pmol L-1 of target miRNA. The recovery rate for miR-126 from fetal bovine serum was 81.5%-109.1%. The biosensor detection of miR-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR （qPCR） results. The use of two AuNP probes further improved the de- tection sensitivity such that even 1 fmol L-t of target miR-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye, The platform simultaneously detected lung cancer related miR-126 and miR-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual miRNA detection.展开更多
Azo dyes are widely applied in the textile industry but are not entirely consumed during the dyeing process and can thus be discharged to the environment in wastewater.However,azo dyes can be degraded using various el...Azo dyes are widely applied in the textile industry but are not entirely consumed during the dyeing process and can thus be discharged to the environment in wastewater.However,azo dyes can be degraded using various electron donors,and in this paper,Acid Orange 7(AO7)degradation performance is investigated using methane(CH4)as the sole electron donor.Methane has multiple sources and is readily available and inexpensive.Experiments using ^(13)C-labeled isotopes showed that AO7 degradation was coupled with anaerobic oxidation of methane(AOM)and,subsequently,affected by the initial concentrations of AO7.Higher concentrations of AO7 could inhibit the activity of microorganisms,which was confirmed by the long-term performance of AO7 degradation,with maximum removal rates of 8.94 mg/(L·d)in a batch reactor and 280 mg/(L·d)in a hollow fiber membrane bioreactor(HfMBR).High-throughput sequencing using 16S rRNA genes showed that Candidatus Methanoperedens,affiliated to ANME-2d,dominated the microbial community in the batch reactor and HfMBR.Additionally,the relative abundance of Proteobacteria bacteria(Phenylobacterium,Pseudomonas,and Geothermobacter)improved after AO7 degradation.This outcome suggested that ANME-2d alone,or acting synergistically with partner bacteria,played a key role in the process of AO7 degradation coupled with AOM.展开更多
Background:As one of the most common diseases in urology,a large number of preclinical studies have been accumulated to explore the etiological mechanism and potential intervention of chronic prostatitis/chronic pelvi...Background:As one of the most common diseases in urology,a large number of preclinical studies have been accumulated to explore the etiological mechanism and potential intervention of chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS).Methods:In this study,we systematically evaluated the current status of preclinical research on CP/CPPS through bibliometrics analysis using VOSviewer and Citespace.Characteristics of publication such as year,country/region,institution,author,journal,citation,and keywords were analyzed.Based on the bibliometrics analysis results of keywords,we summarized the possible mechanisms and promising treatments for CP/CPPS narratively.Results:According to the results of this study,the most common mechanisms involved in CP/CPPS were as follows:Disturbed immune and inflammation mediators,immune cell dysfunction,oxidative stress,dysregulated signaling pathways,apoptosis,gut microbiota,and testosterone metabolism.Traditional Chinese Medicine and extracorporeal shock wave therapy have important potential in the treatment of CP/CPPS.Conclusion:Further translational studies targeting the above mechanisms and validating the objective efficacy of potential treatments indicated by preclinical studies in clinical patients are needed in the future.展开更多
基金supported by Grants from National Key Research and Development Program of China(Nos.2018YFA0108202 and 2017YFA0205300)National Science Foundation of China(Nos.61571429,61571077,61801464,and 61801465)+1 种基金the STS Project of the Chinese Academy of Sciences(No.KFJ-STS-SCYD-120)the Science and Technology Commission of Shanghai Municipality(Nos.16410711800 and 4391901900).
文摘Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for cancer diagnosis in liquid biopsy.Because of their nanoscale size,complex biogenesis,and methodological limitations related to exosome isolation and detection,advancements in their analysis remain slow.Microfluidic technology offers a better analytic approach compared with conventional methods.Here,we developed a bead-based microarray for exosome isolation and multiplexed tumor marker detection.Using this method,exosomes are isolated by binding to antibodies on the bead surface,and tumor markers on the exosomes are detected through quantum dot(QD)probes.The beads are then uniformly trapped and queued among micropillars in the chip.This design benefits fluorescence observation by dispersing the signals into every single bead,thereby avoiding optical interference and enabling more accurate test results.We analyzed exosomes in the cell culture supernatant of lung cancer and endothelial cell lines,and different lung cancer markers labeled with three QD probes were used to conduct multiplexed detection of exosome surface protein markers.Lung cancer-derived samples showed much higher(~sixfold-tenfold)fluorescence intensity than endothelial cell samples,and different types of lung cancer samples showed distinctive marker expression levels.Additionally,using the chip to detect clinical plasma samples from cancer patients showed good diagnostic power and revealed a well consistency with conventional tests for serological markers.These results provide insight into a promising method for exosome tumor marker detection and early-stage cancer diagnosis.
基金supported by the National Key R&D Program of China(2023YFC3304600).
文摘Existing multi-view deep subspace clustering methods aim to learn a unified representation from multi-view data,while the learned representation is difficult to maintain the underlying structure hidden in the origin samples,especially the high-order neighbor relationship between samples.To overcome the above challenges,this paper proposes a novel multi-order neighborhood fusion based multi-view deep subspace clustering model.We creatively integrate the multi-order proximity graph structures of different views into the self-expressive layer by a multi-order neighborhood fusion module.By this design,the multi-order Laplacian matrix supervises the learning of the view-consistent self-representation affinity matrix;then,we can obtain an optimal global affinity matrix where each connected node belongs to one cluster.In addition,the discriminative constraint between views is designed to further improve the clustering performance.A range of experiments on six public datasets demonstrates that the method performs better than other advanced multi-view clustering methods.The code is available at https://github.com/songzuolong/MNF-MDSC(accessed on 25 December 2024).
基金This research was supported by funding from the Shaanxi Provincial Science and Technology Innovation Team(2022TD-15).
文摘Modified starch was better suitable for food processing,and fermentation was one of the effective methods to modify starch.This study investigated the separate and synergistic fermentation of Lactiplantibacillus plantarum and Saccharomyces cerevisiae could whether affect the multi-scale structure and physicochemical properties of highland barley starch(HBS).The results of multi-scale structure determination of HBS showed that compared with unfermented HBS(32.27%),fermentation showed a significant increase in relative crystallinity(35.49-39.81%),while the crystal type of HBS as observed from X-ray diffraction(A-type crystalline pattern)did not change.The small angle X-ray scattering revealed that fermentation reduced the amorphous region and increased the crystalline layer thickness of the HBS.Consequently,the changes in the physicochemical properties showed that the peak viscosity,trough viscosity,and final viscosity of HBS were reduced after fermentation,and the aging of starch gel was delayed significantly.Our results showed that Lactiplantibacillus plantarum and Saccharomyces cerevisiae fermentations improve the physicochemical properties of HBS by modifying the multi-scale structure of starch,especially the synergistic fermentation effect was more effective.
基金supported by the National Basic Research Program of China (2012CB933303)the National Natural Science Foundation of China (61571429, 61571077, 61401442)+2 种基金the Innovation Team of Henan University of Science and Technology (2015XTD003)the Science and Technology Commission of Shanghai Municipality (12441902600, 1402H233900)the Shanghai Clinical Center/Shanghai Xuhui Central Hospital, Chinese Academic of Sciences (BRC2012002)
文摘In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle （AuNP） probes and a gene chip to detect microRNAs （miRNAs）. Specific oligonucleotides were attached to the glass surface as capture probes for the target miRNAs, which were then detected via hybridization to the AuNP probes. The signal was amplified via the re- duction of HAuCI4 by H202. The use of a single AuNP probe detected 10 pmol L-1 of target miRNA. The recovery rate for miR-126 from fetal bovine serum was 81.5%-109.1%. The biosensor detection of miR-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR （qPCR） results. The use of two AuNP probes further improved the de- tection sensitivity such that even 1 fmol L-t of target miR-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye, The platform simultaneously detected lung cancer related miR-126 and miR-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual miRNA detection.
基金supported by the National Natural Science Foundation of China(Grant Nos.51178444 and 51878175)the Program for Innovative Research Team in Science and Technology in Fujian Province University(No.IRTSTFJ)and the Startup Foundation for Introducing Talent of NU1ST.
文摘Azo dyes are widely applied in the textile industry but are not entirely consumed during the dyeing process and can thus be discharged to the environment in wastewater.However,azo dyes can be degraded using various electron donors,and in this paper,Acid Orange 7(AO7)degradation performance is investigated using methane(CH4)as the sole electron donor.Methane has multiple sources and is readily available and inexpensive.Experiments using ^(13)C-labeled isotopes showed that AO7 degradation was coupled with anaerobic oxidation of methane(AOM)and,subsequently,affected by the initial concentrations of AO7.Higher concentrations of AO7 could inhibit the activity of microorganisms,which was confirmed by the long-term performance of AO7 degradation,with maximum removal rates of 8.94 mg/(L·d)in a batch reactor and 280 mg/(L·d)in a hollow fiber membrane bioreactor(HfMBR).High-throughput sequencing using 16S rRNA genes showed that Candidatus Methanoperedens,affiliated to ANME-2d,dominated the microbial community in the batch reactor and HfMBR.Additionally,the relative abundance of Proteobacteria bacteria(Phenylobacterium,Pseudomonas,and Geothermobacter)improved after AO7 degradation.This outcome suggested that ANME-2d alone,or acting synergistically with partner bacteria,played a key role in the process of AO7 degradation coupled with AOM.
基金The National Natural Science Foundation of China,Grant/Award Number:82160148The Cuiying Scientific Training Program for Undergraduates of Lanzhou University Second Hospital,Grant/Award Numbers:CYXZ2021-16,CYXZ2022-41。
文摘Background:As one of the most common diseases in urology,a large number of preclinical studies have been accumulated to explore the etiological mechanism and potential intervention of chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS).Methods:In this study,we systematically evaluated the current status of preclinical research on CP/CPPS through bibliometrics analysis using VOSviewer and Citespace.Characteristics of publication such as year,country/region,institution,author,journal,citation,and keywords were analyzed.Based on the bibliometrics analysis results of keywords,we summarized the possible mechanisms and promising treatments for CP/CPPS narratively.Results:According to the results of this study,the most common mechanisms involved in CP/CPPS were as follows:Disturbed immune and inflammation mediators,immune cell dysfunction,oxidative stress,dysregulated signaling pathways,apoptosis,gut microbiota,and testosterone metabolism.Traditional Chinese Medicine and extracorporeal shock wave therapy have important potential in the treatment of CP/CPPS.Conclusion:Further translational studies targeting the above mechanisms and validating the objective efficacy of potential treatments indicated by preclinical studies in clinical patients are needed in the future.
