Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(H...Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.展开更多
沉香是我国传统名贵南药,广泛应用于医药、香水、香料、宗教等方面,现已形成了工业、文化产业和农业三大类产业,市场价值极高。本研究在中国知网、维普网和万方数据库中以“沉香结香”为主题词检索,导出1978—2022年发表的文章,利用Cite...沉香是我国传统名贵南药,广泛应用于医药、香水、香料、宗教等方面,现已形成了工业、文化产业和农业三大类产业,市场价值极高。本研究在中国知网、维普网和万方数据库中以“沉香结香”为主题词检索,导出1978—2022年发表的文章,利用CiteSpace软件进行可视化分析;在Web of Science以“agarwood production method”为检索词,可视化分析1978—2022年发表的外文文献。结果表明,国内沉香结香的研究一共分为3个阶段,1978—2007年为萌芽阶段,仅有零星文章产出;2008—2017年为快速发展阶段,大量文章产出并在2017年达到顶峰(41篇);2018—2022年为稳定阶段,持续产出文章并维持在年均20篇以上;有关沉香结香机理的研究发文量前10的期刊中,90%等级均在科技核心以上,排在前10的研究结构均处于沉香产业发达地区。国外沉香结香的研究起源于2008年,整体发文量趋势逐年增加;被引量前10的期刊均为SCI;研究量前10的国家均为GDP前列的国家。有关沉香自然结香和人工结香差异分析的研究成为当前研究热点,未来如何利用2种不同沉香结香方式的成分差异发展沉香资源,将会是新的研究趋势。本研究结果将为科研人员研究提供进一步参考方向,也为生产种植者提供沉香结香参考。展开更多
文摘Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.
文摘沉香是我国传统名贵南药,广泛应用于医药、香水、香料、宗教等方面,现已形成了工业、文化产业和农业三大类产业,市场价值极高。本研究在中国知网、维普网和万方数据库中以“沉香结香”为主题词检索,导出1978—2022年发表的文章,利用CiteSpace软件进行可视化分析;在Web of Science以“agarwood production method”为检索词,可视化分析1978—2022年发表的外文文献。结果表明,国内沉香结香的研究一共分为3个阶段,1978—2007年为萌芽阶段,仅有零星文章产出;2008—2017年为快速发展阶段,大量文章产出并在2017年达到顶峰(41篇);2018—2022年为稳定阶段,持续产出文章并维持在年均20篇以上;有关沉香结香机理的研究发文量前10的期刊中,90%等级均在科技核心以上,排在前10的研究结构均处于沉香产业发达地区。国外沉香结香的研究起源于2008年,整体发文量趋势逐年增加;被引量前10的期刊均为SCI;研究量前10的国家均为GDP前列的国家。有关沉香自然结香和人工结香差异分析的研究成为当前研究热点,未来如何利用2种不同沉香结香方式的成分差异发展沉香资源,将会是新的研究趋势。本研究结果将为科研人员研究提供进一步参考方向,也为生产种植者提供沉香结香参考。
