Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wa...Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wang W S et al,2018).The indica subspecies occupies 90%of the rice cultivation area,as opposed to only 10%occupied by the japonica subspecies,which is mainly distributed in northern China,Japan and Korea(Xu and Chen,2016).China is the only country in which japonica and indica are of equal importance.Two-thirds of the rice grown in China is indica,which is cultivated mostly in low-altitude and low-latitude regions,and one-third is japonica,farmed prevalently in high-altitude and high-latitude regions(Wang Y H et al,2018).After the introduction of semi-dwarf gene sdl,the ideal plant architecture breeding and the application of the Fi hybrid,accompanied by the improvement of cultivation methods and field management。展开更多
Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,...Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.展开更多
基金The study was supported by Natural Science Foundation(Grant No.U1708231).The data were provided by the Chinese new rice variety trial(2004-2018).
文摘Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wang W S et al,2018).The indica subspecies occupies 90%of the rice cultivation area,as opposed to only 10%occupied by the japonica subspecies,which is mainly distributed in northern China,Japan and Korea(Xu and Chen,2016).China is the only country in which japonica and indica are of equal importance.Two-thirds of the rice grown in China is indica,which is cultivated mostly in low-altitude and low-latitude regions,and one-third is japonica,farmed prevalently in high-altitude and high-latitude regions(Wang Y H et al,2018).After the introduction of semi-dwarf gene sdl,the ideal plant architecture breeding and the application of the Fi hybrid,accompanied by the improvement of cultivation methods and field management。
基金supported by the Zhejiang Provincial Key Research and Development Plan(Grant No.2019C02006)the National Key Research and Development Program of China(Grant Nos.2016YFD0200804 and 2016YFD0100601-15)+2 种基金the Key Program of Zhejiang Provincial Foundation for Natural Science(Grant No.LZ16C130002)the Zhejiang Fundamental Public Welfare Research Program(Grant No.LGN19C140008)the State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products(Grant No.2010DS700124-ZZ1907).
文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.
