Heterodimerization in RTKs is of vital importance in the RTK signaling and cell functions.Heterodimerization between RTKs can result in diversity of downstream signals,increasing the ability of cells to respond to ext...Heterodimerization in RTKs is of vital importance in the RTK signaling and cell functions.Heterodimerization between RTKs can result in diversity of downstream signals,increasing the ability of cells to respond to external experiments.Traditional RTKs heterodimerization always occur in the same families and is lack of agonists to activate the heterodimeric RTKs signaling pathway.Herein,we developed the DNA agonist based on bivalent aptamers for the heterodimerized RTKs of different families,AF/AM-1,which could simultaneously activate FGFR1 and c-Met signaling.It is the first agonist that realizing the heterodimerization and activation of FGFR1 and c-Met,two different RTK families.The activation of FGFR1/c-Met heterodimer result in the down-stream signals transduction,such as the phosphorylation of Akt and Erk,inducing the cell migration and proliferation.The DNA agonist for RTK heterodimer of different families would have potential applications in the fields of biomedicine.展开更多
基金the National Natural Science Foundation of China(Nos.22104158,82174104,U1903211)the Natural Science Foundation of Hunan Province(No.2021JJ40041)+1 种基金Guangdong Basic and Applied Basic Research Foundation(No.2023A1515011346)Shenzhen Science and Technology Program(No.SZBH202130)。
文摘Heterodimerization in RTKs is of vital importance in the RTK signaling and cell functions.Heterodimerization between RTKs can result in diversity of downstream signals,increasing the ability of cells to respond to external experiments.Traditional RTKs heterodimerization always occur in the same families and is lack of agonists to activate the heterodimeric RTKs signaling pathway.Herein,we developed the DNA agonist based on bivalent aptamers for the heterodimerized RTKs of different families,AF/AM-1,which could simultaneously activate FGFR1 and c-Met signaling.It is the first agonist that realizing the heterodimerization and activation of FGFR1 and c-Met,two different RTK families.The activation of FGFR1/c-Met heterodimer result in the down-stream signals transduction,such as the phosphorylation of Akt and Erk,inducing the cell migration and proliferation.The DNA agonist for RTK heterodimer of different families would have potential applications in the fields of biomedicine.
