Dynamic covalent imine reactions between 2,3-dimethoxy-[1,1:4,1-terphenyl]-3,3,5,5-tetracarbaldehyde(DMTT)and cyclohexanediamine,p-phenylenediamine,and benzidine,respectively,generate a porous organic cage(DMPOC)and t...Dynamic covalent imine reactions between 2,3-dimethoxy-[1,1:4,1-terphenyl]-3,3,5,5-tetracarbaldehyde(DMTT)and cyclohexanediamine,p-phenylenediamine,and benzidine,respectively,generate a porous organic cage(DMPOC)and two covalent organic frameworks(COFs),USTB-29,and USTB-30.DMPOC shows a[3+6]topological cage-like structure according to single crystal X-ray diffraction result.In contrast,both microcrystalline USTB-29 and USTB-30 exhibit two-dimensional monoporous structures in an eclipsed AA stacking style based on powder X-ray diffraction and theoretical simulations.In addition,DMPOC is capable of efficiently absorbing the iodine vapor with an outstanding uptake of 5.10 g/g,much higher than that of USTB-29(3.07 g/g)and USTB-30(3.16 g/g).Cage to COFs transformations have been realized from DMPOC to USTB-29 and USTB-30 via the imine bond exchange with slightly increased iodine vapor uptake.Mechanism investigations uncover that both nitrogen and oxygen atoms of POC and COFs contribute to iodine vapor capture due to the formation of charge transfer matter,and loose interaction introducing adaptive expanding voids of DMPOC is suggested to capture more iodine vapor than that of COFs with strongπ-πinteractions.展开更多
Obtaining absolute pose based on pre-loaded satellite images is one of the important means of autonomous navigation for small Unmanned Aerial Vehicles(UAVs)in Global Navigation Satellite System(GNSS)denied environment...Obtaining absolute pose based on pre-loaded satellite images is one of the important means of autonomous navigation for small Unmanned Aerial Vehicles(UAVs)in Global Navigation Satellite System(GNSS)denied environments.Most of the previous works have tended to build Convolutional Neural Networks(CNNs)to extract features and then directly regress the pose,which will fail when solving the challenges caused by the huge viewpoint and size differences between“UAV-satellite”image pairs in real-world scenarios.Therefore,this paper proposes a probability distribution/regression integrated deep model with the attention-guided triple fusion mechanism,which estimates discrete distributions in pose space and three-dimensional vectors in translation space.In order to overcome the shortage of the relevant dataset,this paper simulates image datasets captured by UAVs with forward-facing cameras during target searching and autonomous attacking.The effectiveness,superiority,and robustness of the proposed method are verified by simulated datasets and flight tests.展开更多
Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples,and male infertility accounts for half of total infertility.Studies on genetically modified animal models hav...Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples,and male infertility accounts for half of total infertility.Studies on genetically modified animal models have identifed lots of genes involved in the pathogenesis of male infertility.The underlying causes,however,remain largely unclear.In this study,we provide evidence that EMCIO,one subunit of endoplasmic reticulum (ER)membrane protein complex (EMC),is required for male fertility.EMC10 is significantly decreased in spermatozoa from patients with asthenozoospermia and positively associated with human sperm motility. Male mice lacking Emc10 gene are completely sterile.Emc10-null spermatozoa exhibit multiple defects including abnormal morphology,decreased motility,impaired capacitation,and impotency of acrosome reaction,thereby which are incapable of fertilizing intact or ZP-free oocytes.However,intracytoplasmic sperm injection could rescue this defect caused by EMC10 deletion. Mechanistically,EMC10 deficiency leads to inactivation of Na/K-ATPase,in turn giving rise to an increased level of intraceltutar Na^+ in spermatozoa,which contributes to decreased sperm motility and abnormal morphology.Other mechanistic investigations demonstrate that the absence of EMC10 results in a reduction of HCO3^- entry and subsequent decreases of both cAMP-dependent protein kinase A substrate phosphorylation and protein tyrosine phosphorytation.These data demonstrate that EMC10 is indispensable to mate fertility via maintaining sperm ion balance of Na^+ and HCO3^-,and also suggest that EMC10 is a promising biomarker for male fertility and a potential pharmaceutical target to treat male infertility.展开更多
The lateral geometry and material property of plasmonic nanostructures are critical parameters for tailoring their optical resonance for sensing applications.While lateral geometry can be easily observed by a scanning...The lateral geometry and material property of plasmonic nanostructures are critical parameters for tailoring their optical resonance for sensing applications.While lateral geometry can be easily observed by a scanning electron microscope or an atomic force microscope,characterizing materials properties of plasmonic devices is not straightforward and requires delicate examination of material composition,cross-sectional thickness,and refractive index.In this study,a deep neural network is adopted to characterize these parameters of unknown plasmonic nanostructures through simple transmission spectra.The network architecture is established based on simulated data to achieve accurate identification of both geometric and material parameters.We then demonstrate that the network training by a mixture of simulated and experimental data can result in correct material property recognition.Our work may indicate a simple and intelligent characterization approach to plasmonic nanostructures by spectroscopic techniques.展开更多
Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.Ho...Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis.展开更多
A green tandem reaction,including insertion/aerobic oxidation/bisindolylation,starting from indoles and diazo compounds has been developed.The combination of water and fluorinated alcohol plays dual roles as solvent a...A green tandem reaction,including insertion/aerobic oxidation/bisindolylation,starting from indoles and diazo compounds has been developed.The combination of water and fluorinated alcohol plays dual roles as solvent and promoter in this chemical transformation.Molecular oxygen in the air acts as an oxidant.3,3’-Bis(indolyl)methanes with quaternary carbon were produced under metal-free conditions.No any catalyst and additive were required.N2 and water were released as sole by-products.Absence of water and fluorinated alcohol resulted in Wolff rearrangement product.展开更多
基金financially supported by the Natural Science Foundation of China(Nos.22235001,22175020,22131005,and 22261132512)Xiaomi Young Scholar Program+1 种基金the Fundamental Research Funds for the Central UniversitiesUniversity of Science and Technology Beijing。
文摘Dynamic covalent imine reactions between 2,3-dimethoxy-[1,1:4,1-terphenyl]-3,3,5,5-tetracarbaldehyde(DMTT)and cyclohexanediamine,p-phenylenediamine,and benzidine,respectively,generate a porous organic cage(DMPOC)and two covalent organic frameworks(COFs),USTB-29,and USTB-30.DMPOC shows a[3+6]topological cage-like structure according to single crystal X-ray diffraction result.In contrast,both microcrystalline USTB-29 and USTB-30 exhibit two-dimensional monoporous structures in an eclipsed AA stacking style based on powder X-ray diffraction and theoretical simulations.In addition,DMPOC is capable of efficiently absorbing the iodine vapor with an outstanding uptake of 5.10 g/g,much higher than that of USTB-29(3.07 g/g)and USTB-30(3.16 g/g).Cage to COFs transformations have been realized from DMPOC to USTB-29 and USTB-30 via the imine bond exchange with slightly increased iodine vapor uptake.Mechanism investigations uncover that both nitrogen and oxygen atoms of POC and COFs contribute to iodine vapor capture due to the formation of charge transfer matter,and loose interaction introducing adaptive expanding voids of DMPOC is suggested to capture more iodine vapor than that of COFs with strongπ-πinteractions.
基金supported by the National Natural Science Foundation of China(No.61973033)the Chongqing Natural Science Foundation,China(No.cstc2021jcyjmsxmX0737).
文摘Obtaining absolute pose based on pre-loaded satellite images is one of the important means of autonomous navigation for small Unmanned Aerial Vehicles(UAVs)in Global Navigation Satellite System(GNSS)denied environments.Most of the previous works have tended to build Convolutional Neural Networks(CNNs)to extract features and then directly regress the pose,which will fail when solving the challenges caused by the huge viewpoint and size differences between“UAV-satellite”image pairs in real-world scenarios.Therefore,this paper proposes a probability distribution/regression integrated deep model with the attention-guided triple fusion mechanism,which estimates discrete distributions in pose space and three-dimensional vectors in translation space.In order to overcome the shortage of the relevant dataset,this paper simulates image datasets captured by UAVs with forward-facing cameras during target searching and autonomous attacking.The effectiveness,superiority,and robustness of the proposed method are verified by simulated datasets and flight tests.
基金the National Basic Research Program (2014CB943103 to Y.Z.,2015CB943003 to Q.D.)the National Natural Science Foundation of China (31471104 and 31671203 to Y.Z.,81370753 to Q.D.,81070647 and 81370936 to X.W.)+1 种基金This work was also sponsored by grants from Shanghai Pujiang Program (16PJ1401700 to X.W.)Science and Technology Commission of Shanghai Municipality (16140901200 to X.W.).
文摘Infertility is a severe public health problem worldwide that prevails up to 15% in reproductive-age couples,and male infertility accounts for half of total infertility.Studies on genetically modified animal models have identifed lots of genes involved in the pathogenesis of male infertility.The underlying causes,however,remain largely unclear.In this study,we provide evidence that EMCIO,one subunit of endoplasmic reticulum (ER)membrane protein complex (EMC),is required for male fertility.EMC10 is significantly decreased in spermatozoa from patients with asthenozoospermia and positively associated with human sperm motility. Male mice lacking Emc10 gene are completely sterile.Emc10-null spermatozoa exhibit multiple defects including abnormal morphology,decreased motility,impaired capacitation,and impotency of acrosome reaction,thereby which are incapable of fertilizing intact or ZP-free oocytes.However,intracytoplasmic sperm injection could rescue this defect caused by EMC10 deletion. Mechanistically,EMC10 deficiency leads to inactivation of Na/K-ATPase,in turn giving rise to an increased level of intraceltutar Na^+ in spermatozoa,which contributes to decreased sperm motility and abnormal morphology.Other mechanistic investigations demonstrate that the absence of EMC10 results in a reduction of HCO3^- entry and subsequent decreases of both cAMP-dependent protein kinase A substrate phosphorylation and protein tyrosine phosphorytation.These data demonstrate that EMC10 is indispensable to mate fertility via maintaining sperm ion balance of Na^+ and HCO3^-,and also suggest that EMC10 is a promising biomarker for male fertility and a potential pharmaceutical target to treat male infertility.
基金supported by the National Natural Science Foundation of China(Nos.61974069,62022043,and 62235008)National Key Research and Development Program of China(No.2021YFA1202904)+2 种基金Natural Science Foundation of Jiangsu Province Major Project(No.BK20212012)State Key Laboratory of Organic Electronics and Information Displays(No.GDX2022010007)Nanjing University of Posts and Telecommunications(Nos.NUPTSF NY219008 and NJUPT 1311 Talent Program).
文摘The lateral geometry and material property of plasmonic nanostructures are critical parameters for tailoring their optical resonance for sensing applications.While lateral geometry can be easily observed by a scanning electron microscope or an atomic force microscope,characterizing materials properties of plasmonic devices is not straightforward and requires delicate examination of material composition,cross-sectional thickness,and refractive index.In this study,a deep neural network is adopted to characterize these parameters of unknown plasmonic nanostructures through simple transmission spectra.The network architecture is established based on simulated data to achieve accurate identification of both geometric and material parameters.We then demonstrate that the network training by a mixture of simulated and experimental data can result in correct material property recognition.Our work may indicate a simple and intelligent characterization approach to plasmonic nanostructures by spectroscopic techniques.
基金the financial support of the National Key Research and Development Program of China(2017YFA0505100)NSF of China(Grants 21974025 and 82121004)for this work.
文摘Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis.
基金financial support from the Ministry of Science and Technology of China (No.2016YFE0132600)Henan Center for Outstanding Overseas Scientists (No. GZS2020001)+1 种基金National Innovation and Entrepreneurship Training Program for College students (No.201910459064)Zhengzhou University
文摘A green tandem reaction,including insertion/aerobic oxidation/bisindolylation,starting from indoles and diazo compounds has been developed.The combination of water and fluorinated alcohol plays dual roles as solvent and promoter in this chemical transformation.Molecular oxygen in the air acts as an oxidant.3,3’-Bis(indolyl)methanes with quaternary carbon were produced under metal-free conditions.No any catalyst and additive were required.N2 and water were released as sole by-products.Absence of water and fluorinated alcohol resulted in Wolff rearrangement product.
