为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲...为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。展开更多
[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with ...[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.展开更多
In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat, six 7-month-old Hainan black goats and six hybrid offsprings were ...In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat, six 7-month-old Hainan black goats and six hybrid offsprings were selected to determine slaughter performance and muscle quality. The results showed that hybridization of Nubian black goat and Hainan black goat significantly improved the production performance and slaughter performance of hybrid offsprings (P>0.05). The meat quality of F1 hybrid black goat had no significant difference with that of Hainan black goat except for the hardness and elasticity (P<0.05). Therefore, the crossbred progenies of Nubian black goat and Hainan black goat have advantages in meat yield and meat performance, and the main meat quality indexes are not significantly different, which meets the market requirements for high-yield and high-quality hybrid combinations, and can be used for cross promotion.展开更多
文摘为了获得鸡热休克蛋白70(heat shock protein 70,HSP70)基因启动子区CpG岛的甲基化状态,初步探索HSP70基因在肌肉组织生长发育中的作用,试验以文昌鸡和北京油鸡为试验材料,采用MassArray质谱法检测鸡胸肌中HSP70基因启动子区CpG岛的甲基化水平,比较分析文昌鸡和北京油鸡HSP70基因的甲基化差异。结果表明:在HSP70基因启动子中共检测到65个CpG位点的甲基化水平;文昌鸡胸肌生长发育过程中有8个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈下降趋势;北京油鸡胸肌生长发育过程中只有3个CpG位点甲基化水平发生明显改变,其甲基化密度从第30天到第120天基本呈升高趋势,第120天时甲基化水平达到最高。说明文昌鸡与北京油鸡HSP70基因启动子区CpG岛的甲基化模式和水平不同,这可能导致两种鸡对应激反应产生不同的耐受程度。
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)
文摘[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01)Youth Science and Technology Talents Innovation Project of Hainan Association for Science and Technology(QCXM201802)
文摘In order to compare the slaughter performance and meat quality of Hainan black goats and hybrid offsprings of Nubian Black Goat and Hainan Black Goat, six 7-month-old Hainan black goats and six hybrid offsprings were selected to determine slaughter performance and muscle quality. The results showed that hybridization of Nubian black goat and Hainan black goat significantly improved the production performance and slaughter performance of hybrid offsprings (P>0.05). The meat quality of F1 hybrid black goat had no significant difference with that of Hainan black goat except for the hardness and elasticity (P<0.05). Therefore, the crossbred progenies of Nubian black goat and Hainan black goat have advantages in meat yield and meat performance, and the main meat quality indexes are not significantly different, which meets the market requirements for high-yield and high-quality hybrid combinations, and can be used for cross promotion.
