Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isofla...Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isoflavone content in soybean was detected on chromosome(Chr.) 05 in a recombinant inbred line(RIL) population from a cross of Huachun 2×Wayao. In this study, the parental lines were re-sequenced using the Illumina Solexa System with deep coverage. A total of 63,099 polymorphic long insertions and deletions(InDels)(≥15 bp)were identified between the parents Huachun 2 and Wayao. The InDels were unevenly distributed on 20chromosomes of soybean, varying from 1,826 in Chr. 12 to 4,544 in Chr. 18. A total of 10,002 long InDels(15.85% of total) were located in genic regions, including 1,139 large-effect long InDels which resulted in truncated or elongated protein sequences. In the qIF05-1 region, 68 long InDels were detected between the two parents. Using a progeny recombination experiment and genotype analysis, the qIF05-1 locus was mapped into a 102.2 kb genomic region, and this region contained 12 genes. By RNA-seq data analysis, genome sequence comparison and functional validation through ectopic expression in Arabidopsis thaliana, Glyma.05G208300(described as GmEGL3), which is a basic helix-loop-helix(bHLH) transcription factor in plants, emerged as the most likely confirmed gene in qIF05-1. These long InDels can be used as a type of complementary genetic method for QTL fine mapping, and they can facilitate genetic studies and molecular-assisted selection breeding in soybean.展开更多
目的观察应用脑膜中动脉(MMA)栓塞术治疗慢性硬脑膜下血肿(cSDH)的临床疗效,并探讨cSDH患者可单纯应用MMA栓塞治疗成功的影响因素。方法回顾性连续纳入北京医院神经外科2021年7月至2023年11月间应用MMA栓塞治疗的33例cSDH患者,收集患者...目的观察应用脑膜中动脉(MMA)栓塞术治疗慢性硬脑膜下血肿(cSDH)的临床疗效,并探讨cSDH患者可单纯应用MMA栓塞治疗成功的影响因素。方法回顾性连续纳入北京医院神经外科2021年7月至2023年11月间应用MMA栓塞治疗的33例cSDH患者,收集患者的人口学、临床和影像学资料,观察手术疗效及围手术期并发症情况,并进行临床和影像随访。根据是否联合应用钻孔引流术将患者分为单纯栓塞组和联合钻孔引流组,采用单因素分析(Fisher精确概率检验、t检验或Mann-Whitney U检验)进行组间上述资料的差异性比较,并应用多因素Logistic回归分析可单纯应用MMA栓塞治疗获得成功的影响因素。结果共33例患者43侧cSDH,22例患者单纯行MMA栓塞治疗成功,11例联合应用钻孔引流术。所有患者均取得技术上成功,1例患者术后发生面瘫,并发症发生率为3.0%(1/33)。共29例患者获得术后至少2周影像学随访,影像随访时间92.0(52.5,161.0)d,26例(89.7%)末次影像随访时cSDH最大血肿厚度较术前减少50%以上。33例患者临床随访时间为(304.9±190.5)d,93.9%(31/33)的患者神经功能较术前稳定或好转,无病例血肿复发。与联合钻孔引流组相比,单纯栓塞组患者年龄更小[(71.9±9.7)岁vs(81.4±9.9)岁,t=2.620,P=0.013],术前改良Rankin量表评分更低[2(1,3)分vs 4(2,4)分,Z=2.471,P=0.017],术前中线移位程度更轻[4.5(1.5,6.0)mm vs 8.0(7.0,9.0)mm,Z=3.618,P<0.001]。多因素Logistic回归分析结果显示,年龄较小(OR=0.63,95%CI:0.41~0.97,P=0.038)和术前中线移位程度较轻(OR=0.07,95%CI:0.01~0.72,P=0.026)是单纯MMA栓塞治疗成功的独立预测因素。结论初步观察显示,应用MMA栓塞治疗cSDH是安全有效的,并可作为首要治疗方式。年龄较小和中线移位程度较轻是cSDH患者可单纯应用MMA栓塞治疗成功的独立预测因素。展开更多
Background:Endovascular treatment(EVT)is an alternative option for symptomatic intracranial atherosclerotic disease(ICAD).However,the effect of EVT treatment on ICAD plaques is still unclear.This study describes the I...Background:Endovascular treatment(EVT)is an alternative option for symptomatic intracranial atherosclerotic disease(ICAD).However,the effect of EVT treatment on ICAD plaques is still unclear.This study describes the ICAD plaque characteristics after EVT treatment and analyzes the effect of different EVT treatments on plaque characteristics.Method:From 2017 January to 2022 January,ICAD patients who underwent endovascular treatment and had follow-up high-resolution magnetic resonance image(HRMRI)were enrolled in the study.Multiple plaque characteristics,including plaque enhancement,plaque burden,were measured based on preoperative,and follow-up HRMRI.Plaque characteristics and postoperative plaque changes were analyzed between different treatment groups.Result:Finally,50 intracranial atherosclerotic plaques in 45 patients were included.Including 28 male patients and 17 female,media age 63.0 years old.Among 50 plaques,41 received percutaneous angioplasty(including 22 plain balloons and 19 drug-coated balloons(DCB))and the other 9 underwent stenting.Stenosis rate,plaque burden and eccentricity index at the lesion site were significantly decreased after EVT compared with preoperative periods(p<0.001).And only the DCB group showed a significant reduction in plaque enhancement at follow-up(p<0.001).No significant preoperative and postoperative changes in other plaque characteristics were found.Conclusion:EVT treatment could compromise the characteristics of intracranial periarterial atherosclerotic plaques,and DCB treatment may result in a reduction in plaque enhancement after treatment.展开更多
基金supported by the China Agriculture Research System of MOF and MARA(CARS-04-PS12)the Research and Development Program in the Key-Areas of Guangdong Province,China(2020B020220008)the Guangdong Agricultural Research System,China(2023KJ136-03).
文摘Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isoflavone content in soybean was detected on chromosome(Chr.) 05 in a recombinant inbred line(RIL) population from a cross of Huachun 2×Wayao. In this study, the parental lines were re-sequenced using the Illumina Solexa System with deep coverage. A total of 63,099 polymorphic long insertions and deletions(InDels)(≥15 bp)were identified between the parents Huachun 2 and Wayao. The InDels were unevenly distributed on 20chromosomes of soybean, varying from 1,826 in Chr. 12 to 4,544 in Chr. 18. A total of 10,002 long InDels(15.85% of total) were located in genic regions, including 1,139 large-effect long InDels which resulted in truncated or elongated protein sequences. In the qIF05-1 region, 68 long InDels were detected between the two parents. Using a progeny recombination experiment and genotype analysis, the qIF05-1 locus was mapped into a 102.2 kb genomic region, and this region contained 12 genes. By RNA-seq data analysis, genome sequence comparison and functional validation through ectopic expression in Arabidopsis thaliana, Glyma.05G208300(described as GmEGL3), which is a basic helix-loop-helix(bHLH) transcription factor in plants, emerged as the most likely confirmed gene in qIF05-1. These long InDels can be used as a type of complementary genetic method for QTL fine mapping, and they can facilitate genetic studies and molecular-assisted selection breeding in soybean.
文摘目的观察应用脑膜中动脉(MMA)栓塞术治疗慢性硬脑膜下血肿(cSDH)的临床疗效,并探讨cSDH患者可单纯应用MMA栓塞治疗成功的影响因素。方法回顾性连续纳入北京医院神经外科2021年7月至2023年11月间应用MMA栓塞治疗的33例cSDH患者,收集患者的人口学、临床和影像学资料,观察手术疗效及围手术期并发症情况,并进行临床和影像随访。根据是否联合应用钻孔引流术将患者分为单纯栓塞组和联合钻孔引流组,采用单因素分析(Fisher精确概率检验、t检验或Mann-Whitney U检验)进行组间上述资料的差异性比较,并应用多因素Logistic回归分析可单纯应用MMA栓塞治疗获得成功的影响因素。结果共33例患者43侧cSDH,22例患者单纯行MMA栓塞治疗成功,11例联合应用钻孔引流术。所有患者均取得技术上成功,1例患者术后发生面瘫,并发症发生率为3.0%(1/33)。共29例患者获得术后至少2周影像学随访,影像随访时间92.0(52.5,161.0)d,26例(89.7%)末次影像随访时cSDH最大血肿厚度较术前减少50%以上。33例患者临床随访时间为(304.9±190.5)d,93.9%(31/33)的患者神经功能较术前稳定或好转,无病例血肿复发。与联合钻孔引流组相比,单纯栓塞组患者年龄更小[(71.9±9.7)岁vs(81.4±9.9)岁,t=2.620,P=0.013],术前改良Rankin量表评分更低[2(1,3)分vs 4(2,4)分,Z=2.471,P=0.017],术前中线移位程度更轻[4.5(1.5,6.0)mm vs 8.0(7.0,9.0)mm,Z=3.618,P<0.001]。多因素Logistic回归分析结果显示,年龄较小(OR=0.63,95%CI:0.41~0.97,P=0.038)和术前中线移位程度较轻(OR=0.07,95%CI:0.01~0.72,P=0.026)是单纯MMA栓塞治疗成功的独立预测因素。结论初步观察显示,应用MMA栓塞治疗cSDH是安全有效的,并可作为首要治疗方式。年龄较小和中线移位程度较轻是cSDH患者可单纯应用MMA栓塞治疗成功的独立预测因素。
基金funded by the Beijing Hospital Clinical Research 121 Project(BJ-2018-086 and BJ-2018-202)Capital’s Funds for Health Improvement and Research(2020-4-4053)
文摘Background:Endovascular treatment(EVT)is an alternative option for symptomatic intracranial atherosclerotic disease(ICAD).However,the effect of EVT treatment on ICAD plaques is still unclear.This study describes the ICAD plaque characteristics after EVT treatment and analyzes the effect of different EVT treatments on plaque characteristics.Method:From 2017 January to 2022 January,ICAD patients who underwent endovascular treatment and had follow-up high-resolution magnetic resonance image(HRMRI)were enrolled in the study.Multiple plaque characteristics,including plaque enhancement,plaque burden,were measured based on preoperative,and follow-up HRMRI.Plaque characteristics and postoperative plaque changes were analyzed between different treatment groups.Result:Finally,50 intracranial atherosclerotic plaques in 45 patients were included.Including 28 male patients and 17 female,media age 63.0 years old.Among 50 plaques,41 received percutaneous angioplasty(including 22 plain balloons and 19 drug-coated balloons(DCB))and the other 9 underwent stenting.Stenosis rate,plaque burden and eccentricity index at the lesion site were significantly decreased after EVT compared with preoperative periods(p<0.001).And only the DCB group showed a significant reduction in plaque enhancement at follow-up(p<0.001).No significant preoperative and postoperative changes in other plaque characteristics were found.Conclusion:EVT treatment could compromise the characteristics of intracranial periarterial atherosclerotic plaques,and DCB treatment may result in a reduction in plaque enhancement after treatment.
