The more frequent occurrence and severer drought events resulting from climate change are increasingly affecting the physiological performance of trees and ecosystem carbon sequestration in many regions of the world.H...The more frequent occurrence and severer drought events resulting from climate change are increasingly affecting the physiological performance of trees and ecosystem carbon sequestration in many regions of the world.However,our understanding of the mechanisms underlying the responses and adaption of forest trees to prolonged and multi-year drought is still limited.To address this problem,we conducted a long-term manipulative throughfall reduction(TFR,reduction of natural throughfall by 50%–70%during growing seasons)experiment in a natural oriental white oak(Quercus aliena var.acuteserrata Maxim.)forest under warm-temperate climate.After seven years of continuous TFR treatment,the aboveground growth in Q.aliena var.acuteserrata started to decline.Compared with the control plots,trees in the TFR treatment significantly reduced growth increments of stems(14.2%)and leaf area index(6.8%).The rate of net photosynthesis appeared to be more susceptible to changes in soil water in trees subjected to the TFR than in the control.The TFR-treated trees allocated significantly more photosynthates to belowground,leading to enhanced growth and nonstructural carbohydrates(NSC)storage in roots.The 7-year continuous TFR treatment increased the biomass,the production and the NSC concentration in the fine roots by 53.6%,153.6%and 9.6%,respectively.There were clear trade-offs between the aboveground growth and the fine root biomass and NSC storage in Q.aliena var.acuteserrata trees in response to the multi-year TFR treatment.A negative correlation between the fine root NSC concentration and soil water suggested a strategy of preferential C storage over growth when soil water became deficient;the stored NSC during water limitation would then help promote root growth when drought stress is released.Our findings demonstrate the warm-temperate oak forest adopted a more conservative NSC use strategy in response to long-term drought stress,with enhanced root growth and NSC storage at the expenses of above-ground growth to mitigate climate changeinduced drought.展开更多
Objectives:Harvesting fruits prematurely is a serious problem in many Camellia oleifera‘Huaxin’orchards.Systematic views of dynamic alterations in central metabolism associated with the oil content and oil quality o...Objectives:Harvesting fruits prematurely is a serious problem in many Camellia oleifera‘Huaxin’orchards.Systematic views of dynamic alterations in central metabolism associated with the oil content and oil quality of this cultivar at different harvesting dates were investigated.Materials and Methods:Oil trait analysis was performed in conjunction with data-independent acquisition proteomics analysis using kernels harvested at three time points.Samples were taken from different years and locations to verify oil trait changes,and parallel reaction monitoring(PRM)was performed for some pivotal proteins to validate changes in their abundance.Results:Samples harvested at 6 d after the recommended harvesting date had the highest contents and best fatty acid composition of Camellia oil.A total of 614 differentially accumulated proteins were detected in three pairwise comparisons.Six days prior to the recommended harvesting date,the maximum expression of ribonucloprotein,helicase ATP-binding domain-containing protein,and glycerol kinase and the highest seed moisture content were observed.The highest mitogen-activated protein kinase 6,very-long-chain 3-oxoacyl-CoA reductase and stearoyl-CoA desaturase expression appeared on the recommended harvesting date.A delayed effect was observed for proteomic and oil trait data.Oil trait and PRM validations indicated that the results were highly reliable.Conclusions:The current data strongly suggest that in Guizhou Province,‘Huaxin’cannot be harvested earlier than the recommended harvesting date;appropriately delaying the harvesting time can improve the ultimate production and quality of Camellia oil.展开更多
基金supported by the National Key Research and Development Program of China,China(No.2021YFD2200405)National Natural Science Foundation of China,China(No.31930078)+1 种基金the Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2020QB009)the special funding for long term forest ecosystem research from National Forestry and Grassland Administration and Ecology and Nature Conservation Institute,Chinese Academy of Forestry.
文摘The more frequent occurrence and severer drought events resulting from climate change are increasingly affecting the physiological performance of trees and ecosystem carbon sequestration in many regions of the world.However,our understanding of the mechanisms underlying the responses and adaption of forest trees to prolonged and multi-year drought is still limited.To address this problem,we conducted a long-term manipulative throughfall reduction(TFR,reduction of natural throughfall by 50%–70%during growing seasons)experiment in a natural oriental white oak(Quercus aliena var.acuteserrata Maxim.)forest under warm-temperate climate.After seven years of continuous TFR treatment,the aboveground growth in Q.aliena var.acuteserrata started to decline.Compared with the control plots,trees in the TFR treatment significantly reduced growth increments of stems(14.2%)and leaf area index(6.8%).The rate of net photosynthesis appeared to be more susceptible to changes in soil water in trees subjected to the TFR than in the control.The TFR-treated trees allocated significantly more photosynthates to belowground,leading to enhanced growth and nonstructural carbohydrates(NSC)storage in roots.The 7-year continuous TFR treatment increased the biomass,the production and the NSC concentration in the fine roots by 53.6%,153.6%and 9.6%,respectively.There were clear trade-offs between the aboveground growth and the fine root biomass and NSC storage in Q.aliena var.acuteserrata trees in response to the multi-year TFR treatment.A negative correlation between the fine root NSC concentration and soil water suggested a strategy of preferential C storage over growth when soil water became deficient;the stored NSC during water limitation would then help promote root growth when drought stress is released.Our findings demonstrate the warm-temperate oak forest adopted a more conservative NSC use strategy in response to long-term drought stress,with enhanced root growth and NSC storage at the expenses of above-ground growth to mitigate climate changeinduced drought.
基金supported by the Natural Science Foundation of China(No.32260376)the Natural Science Foundation of Guizhou Province(No.QKHJC-ZK[2021]YB159)+2 种基金the Doctoral Scientific Research Start-up Foundation from Tongren University(Nos.trxyDH1910,trxyDH1911)the Natural Science Foundation from the Bureau of Education,Guizhou Province(Nos.QJKY[2020]068,QJKY[2020]171,QJKY[2019]052)the Science and Technology Supporting Project from the Bureau of Science&Technology,Tongren City(Nos.TSKY[2020]125,TSKY[2019]084),China.
文摘Objectives:Harvesting fruits prematurely is a serious problem in many Camellia oleifera‘Huaxin’orchards.Systematic views of dynamic alterations in central metabolism associated with the oil content and oil quality of this cultivar at different harvesting dates were investigated.Materials and Methods:Oil trait analysis was performed in conjunction with data-independent acquisition proteomics analysis using kernels harvested at three time points.Samples were taken from different years and locations to verify oil trait changes,and parallel reaction monitoring(PRM)was performed for some pivotal proteins to validate changes in their abundance.Results:Samples harvested at 6 d after the recommended harvesting date had the highest contents and best fatty acid composition of Camellia oil.A total of 614 differentially accumulated proteins were detected in three pairwise comparisons.Six days prior to the recommended harvesting date,the maximum expression of ribonucloprotein,helicase ATP-binding domain-containing protein,and glycerol kinase and the highest seed moisture content were observed.The highest mitogen-activated protein kinase 6,very-long-chain 3-oxoacyl-CoA reductase and stearoyl-CoA desaturase expression appeared on the recommended harvesting date.A delayed effect was observed for proteomic and oil trait data.Oil trait and PRM validations indicated that the results were highly reliable.Conclusions:The current data strongly suggest that in Guizhou Province,‘Huaxin’cannot be harvested earlier than the recommended harvesting date;appropriately delaying the harvesting time can improve the ultimate production and quality of Camellia oil.
