Osthole, an effective monomer in Chinese medicinal herbs, can cross the blood-brain barrier and protect against brain injury, with few toxic effects. In this study, a rat model of Alzheimer＇s disease was established ...Osthole, an effective monomer in Chinese medicinal herbs, can cross the blood-brain barrier and protect against brain injury, with few toxic effects. In this study, a rat model of Alzheimer＇s disease was established after intracerebroventricular injection of β-amyloid peptide （25-35）. Subsequently the rats were intraperitoneally treated with osthole （12.5 or 25.0 mg/kg） for 14 successive days. Results showed that osthole treatment significantly improved cognitive impairment and protected hippocampal neurons of AIzheimer＇s disease rats. Also, osthole treatment alleviated suppressed long-term potentiation in the hippocampus of Alzheimer＇s disease rats. In these osthole-treated Alzheimer＇s disease rats, the level of glutamate decreased, but there was no significant change in y-amino-butyric acid. These experimental findings suggest that osthole can improve learning and memory impairment, and increase synaptic plasticity in Alzheimer＇s disease rats. These effects of osthole may be because of its regulation of central glutamate and y-amino-butyric acid levels.展开更多
Infection and poor tissue repair are the key causes of percutaneous implantation failure. However, there is a lackof effective strategies to cope with due to its high requirements of sterilization, soft tissue healing...Infection and poor tissue repair are the key causes of percutaneous implantation failure. However, there is a lackof effective strategies to cope with due to its high requirements of sterilization, soft tissue healing, andosseointegration. In this work, L-arginine (L-Arg) was loaded onto a sulfonated polyetheretherketone (PEEK)surface to solve this issue. Under the infection condition, nitric oxide (NO) and reactive oxygen species (ROS) areproduced through catalyzing L-Arg by inducible nitric oxide synthase (iNOS) and thus play a role in bacteriasterilization. Under the tissue repair condition, L-Arg is catalyzed to ornithine by Arginase-1 (Arg-1), whichpromotes the proliferation and collagen secretion of L929 and rBMSCs. Notably, L-Arg loading samples couldpolarize macrophages to M1 and M2 in infection and tissue repair conditions, respectively. The results in vivoshow that the L-Arg loading samples could enhance infected soft tissue sealing and bone regeneration. Insummary, L-Arg loading sulfonated PEEK could polarize macrophage through metabolic reprogramming,providing multi-functions of antibacterial abilities, soft tissue repair, and bone regeneration, which gives a newidea to design percutaneous implantation materials.展开更多
目的评价生物陶瓷材料iRoot BP Plus应用于成熟恒牙不可复性牙髓炎冠髓切断术(FP)的治疗效果。方法收集2020年6月至2021年10月期间于广西医科大学附属口腔医院综合一门诊就诊,被诊断为“不可复性牙髓炎”,且在局部麻醉下使用iRoot BP P...目的评价生物陶瓷材料iRoot BP Plus应用于成熟恒牙不可复性牙髓炎冠髓切断术(FP)的治疗效果。方法收集2020年6月至2021年10月期间于广西医科大学附属口腔医院综合一门诊就诊,被诊断为“不可复性牙髓炎”,且在局部麻醉下使用iRoot BP Plus为盖髓剂进行FP的病例进行回顾性研究。随访资料收集至术后2年,通过对患者基本情况、术前病因、临床及影像学检查结果、诊断、术中治疗细节和术后临床体征,以及术后6、12、24个月影像学检查结果等资料数据进行汇总,统计各变量的例数及占比,评价其临床和影像学疗效。结果共收集25例患者(28颗患牙),其中男8例(32%)、女17例(68%),平均年龄(35.5±15.5)岁。术后3~24个月临床检查显示,患者原有症状消失且均无新发临床症状;术前7例根尖周阴影患者及2例牙周膜异常增宽患者在术后随访至12个月时均实现根尖阴影消失和牙周膜间隙恢复正常,其余16例术前根尖周影像正常患者均无新发根尖周异常。末次随访时,26颗患牙牙髓断面处见钙化桥形成,2颗患牙牙髓断面处未见明显钙化组织。结论iRoot BP Plus作为盖髓剂应用于成熟恒牙不可复性牙髓炎FP可取得良好的治疗效果。展开更多
基金supported by the Natural Science Foundation of Hebei Province, No. 2004000653Key Project of Hebei Province Health Department, No. 200901830
文摘Osthole, an effective monomer in Chinese medicinal herbs, can cross the blood-brain barrier and protect against brain injury, with few toxic effects. In this study, a rat model of Alzheimer＇s disease was established after intracerebroventricular injection of β-amyloid peptide （25-35）. Subsequently the rats were intraperitoneally treated with osthole （12.5 or 25.0 mg/kg） for 14 successive days. Results showed that osthole treatment significantly improved cognitive impairment and protected hippocampal neurons of AIzheimer＇s disease rats. Also, osthole treatment alleviated suppressed long-term potentiation in the hippocampus of Alzheimer＇s disease rats. In these osthole-treated Alzheimer＇s disease rats, the level of glutamate decreased, but there was no significant change in y-amino-butyric acid. These experimental findings suggest that osthole can improve learning and memory impairment, and increase synaptic plasticity in Alzheimer＇s disease rats. These effects of osthole may be because of its regulation of central glutamate and y-amino-butyric acid levels.
基金the National Natural Science Foundation of China(32371397,32000938,U21A20100)The Fundamental Research Funds for The Central Universities(YG2023ZD29)+2 种基金Shenzhen Science and Technology Funding(JCYJ20210324120009026)Laboratory Open Fund of Key Technology and Materials in Minimally Invasive Spine Surgery(2024JZWC-ZDB03,2024JZWC-YBA04)Talent project of Shanghai Tongren Hospital(TRKYRC-xx02)are acknowledged.
文摘Infection and poor tissue repair are the key causes of percutaneous implantation failure. However, there is a lackof effective strategies to cope with due to its high requirements of sterilization, soft tissue healing, andosseointegration. In this work, L-arginine (L-Arg) was loaded onto a sulfonated polyetheretherketone (PEEK)surface to solve this issue. Under the infection condition, nitric oxide (NO) and reactive oxygen species (ROS) areproduced through catalyzing L-Arg by inducible nitric oxide synthase (iNOS) and thus play a role in bacteriasterilization. Under the tissue repair condition, L-Arg is catalyzed to ornithine by Arginase-1 (Arg-1), whichpromotes the proliferation and collagen secretion of L929 and rBMSCs. Notably, L-Arg loading samples couldpolarize macrophages to M1 and M2 in infection and tissue repair conditions, respectively. The results in vivoshow that the L-Arg loading samples could enhance infected soft tissue sealing and bone regeneration. Insummary, L-Arg loading sulfonated PEEK could polarize macrophage through metabolic reprogramming,providing multi-functions of antibacterial abilities, soft tissue repair, and bone regeneration, which gives a newidea to design percutaneous implantation materials.
文摘目的评价生物陶瓷材料iRoot BP Plus应用于成熟恒牙不可复性牙髓炎冠髓切断术(FP)的治疗效果。方法收集2020年6月至2021年10月期间于广西医科大学附属口腔医院综合一门诊就诊,被诊断为“不可复性牙髓炎”,且在局部麻醉下使用iRoot BP Plus为盖髓剂进行FP的病例进行回顾性研究。随访资料收集至术后2年,通过对患者基本情况、术前病因、临床及影像学检查结果、诊断、术中治疗细节和术后临床体征,以及术后6、12、24个月影像学检查结果等资料数据进行汇总,统计各变量的例数及占比,评价其临床和影像学疗效。结果共收集25例患者(28颗患牙),其中男8例(32%)、女17例(68%),平均年龄(35.5±15.5)岁。术后3~24个月临床检查显示,患者原有症状消失且均无新发临床症状;术前7例根尖周阴影患者及2例牙周膜异常增宽患者在术后随访至12个月时均实现根尖阴影消失和牙周膜间隙恢复正常,其余16例术前根尖周影像正常患者均无新发根尖周异常。末次随访时,26颗患牙牙髓断面处见钙化桥形成,2颗患牙牙髓断面处未见明显钙化组织。结论iRoot BP Plus作为盖髓剂应用于成熟恒牙不可复性牙髓炎FP可取得良好的治疗效果。
