BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain...BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain to be further explored.AIM The aim was to reveal the possible mechanisms by which hypoxia enhances the proliferation of hP-MSCs.METHODS A hypoxic cell incubator(2.5%O2)was used to mimic a hypoxic microenvironment.Cell counting kit-8 and 5-ethynyl-20-deoxyuridine incorporation assays were used to assay the proliferation of hP-MSCs.The cell cycle was profiled by flow cytometry.Transcriptome profiling of hP-MSCs under hypoxia was performed by RNA sequencing.CD99 mRNA expression was assayed by reverse transcription-polymerase chain reaction.Small interfering RNA-mediated hypoxia-inducible factor 1α(HIF-1α)or CD99 knockdown of hP-MSCs,luciferase reporter assays,and the ERK1/2 signaling inhibitor PD98059 were used in the mechanistic analysis.Protein expression was assayed by western blotting;immunofluorescence assays were conducted to evaluate changes in expression levels.RESULTS Hypoxia enhanced hP-MSC proliferation,increased the expression of cyclin E1,cyclin-dependent kinase 2,and cyclin A2,and decreased the expression of p21.Under hypoxia,CD99 expression was increased by HIF-1α.CD99-specific small interfering RNA or the ERK1/2 signaling inhibitor PD98059 abrogated the hypoxia-induced increase in cell proliferation.CONCLUSION Hypoxia promoted hP-MSCs proliferation in a manner dependent on CD99 regulation of the MAPK/ERK signaling pathway in vitro.展开更多
Reportedly,maxillary premolars are the most complex teeth for performing endodontic treatment,[1] with root canals varying based on race,genetic predisposition,diet,region,gender,[2] and perhaps age.To date,few detail...Reportedly,maxillary premolars are the most complex teeth for performing endodontic treatment,[1] with root canals varying based on race,genetic predisposition,diet,region,gender,[2] and perhaps age.To date,few detailed studies have been conducted to assess number of roots and root canal morphology of maxillary premolars in the elderly.The present study analyzed root canal morphology of maxillary premolars in elderly individuals using cone-beam computed tomography （CBCT）,which has substantial clinical value in guiding endodontic treatment.[3]展开更多
To the Editor： Kaposi＇s varicelliform eruption （KVE） is a distinct viral skin disease which is characterized by disseminated vesiculopustules and erosions superimposed on the preexisting dermatosis. It is mostly c...To the Editor： Kaposi＇s varicelliform eruption （KVE） is a distinct viral skin disease which is characterized by disseminated vesiculopustules and erosions superimposed on the preexisting dermatosis. It is mostly caused by herpes simplex virus （HSV） type 1 or 2 and rarely by vaccine-virus or Coxsackie A16 virus. The KVE is normally described in atopic dermatitis （AD）, but also observed in association with papulosquamous or acantholytic dermatosis. Report about rosacea complicated with KVE is rare. On the other hand, tacrolimus ointment has been demonstrated to be an effective treatment for steroid-induced rosacea. Here, we report a case of KVE in a patient with rosacea during long-term treatment with 0.03% tacrolimus ointment.展开更多
基金Stem Cell and Translational Research from the National Key Research and Development Program of China,No.2020YFA0113003National Natural Science Foundation of China, No. 81971756.
文摘BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain to be further explored.AIM The aim was to reveal the possible mechanisms by which hypoxia enhances the proliferation of hP-MSCs.METHODS A hypoxic cell incubator(2.5%O2)was used to mimic a hypoxic microenvironment.Cell counting kit-8 and 5-ethynyl-20-deoxyuridine incorporation assays were used to assay the proliferation of hP-MSCs.The cell cycle was profiled by flow cytometry.Transcriptome profiling of hP-MSCs under hypoxia was performed by RNA sequencing.CD99 mRNA expression was assayed by reverse transcription-polymerase chain reaction.Small interfering RNA-mediated hypoxia-inducible factor 1α(HIF-1α)or CD99 knockdown of hP-MSCs,luciferase reporter assays,and the ERK1/2 signaling inhibitor PD98059 were used in the mechanistic analysis.Protein expression was assayed by western blotting;immunofluorescence assays were conducted to evaluate changes in expression levels.RESULTS Hypoxia enhanced hP-MSC proliferation,increased the expression of cyclin E1,cyclin-dependent kinase 2,and cyclin A2,and decreased the expression of p21.Under hypoxia,CD99 expression was increased by HIF-1α.CD99-specific small interfering RNA or the ERK1/2 signaling inhibitor PD98059 abrogated the hypoxia-induced increase in cell proliferation.CONCLUSION Hypoxia promoted hP-MSCs proliferation in a manner dependent on CD99 regulation of the MAPK/ERK signaling pathway in vitro.
文摘Reportedly,maxillary premolars are the most complex teeth for performing endodontic treatment,[1] with root canals varying based on race,genetic predisposition,diet,region,gender,[2] and perhaps age.To date,few detailed studies have been conducted to assess number of roots and root canal morphology of maxillary premolars in the elderly.The present study analyzed root canal morphology of maxillary premolars in elderly individuals using cone-beam computed tomography （CBCT）,which has substantial clinical value in guiding endodontic treatment.[3]
文摘To the Editor： Kaposi＇s varicelliform eruption （KVE） is a distinct viral skin disease which is characterized by disseminated vesiculopustules and erosions superimposed on the preexisting dermatosis. It is mostly caused by herpes simplex virus （HSV） type 1 or 2 and rarely by vaccine-virus or Coxsackie A16 virus. The KVE is normally described in atopic dermatitis （AD）, but also observed in association with papulosquamous or acantholytic dermatosis. Report about rosacea complicated with KVE is rare. On the other hand, tacrolimus ointment has been demonstrated to be an effective treatment for steroid-induced rosacea. Here, we report a case of KVE in a patient with rosacea during long-term treatment with 0.03% tacrolimus ointment.
