The newly-discovered Donglufang Moe Cu porphyry-skarn deposit is located in the southern Yidun Terrane, southeast Tibet, with more than 80 million tonnes(Mt) of reserves(grading 0.15 wt.% Mo and0.48 wt.% Cu) hosted in...The newly-discovered Donglufang Moe Cu porphyry-skarn deposit is located in the southern Yidun Terrane, southeast Tibet, with more than 80 million tonnes(Mt) of reserves(grading 0.15 wt.% Mo and0.48 wt.% Cu) hosted in Triassic strata and Late Cretaceous granodiorite porphyry. Ree Os dating of molybdenum ore yielded a weighted mean age of 84.9 ± 1.0 Ma and an isochron age of 85.2 ± 0.6 Ma.LA-ICP-MS Ue Pb dating of zircons from the granodiorite porphyry yielded206 Pb/238 U ages ranging from 87.4 Ma to 84.2 Ma with a weighted mean206 Pb/238 U age of 85.1 ±0.5 Ma, indicating a temporal linkage between granitic magmatism and Moe Cu mineralization. Geochemical analyses show that the granodiorite porphyries are I-type granites with Si O_2 contents of 64.3 -66.7 wt.%. These rocks are typically metaluminous with high K_2 O/Na_2 O ratios, low Mg O(1.32 -1.56 wt.%), Cr(5.6 -12.9 ppm), Ni(3.79 -10.81 ppm), Mg#(43 -52) values, and high Sr(304 -844 ppm), Sr/Y(21.2 -50.8) and La/Yb ratios(37.0 -60.1). They are enriched in light rare-earth elements(LREE) relative to heavy rare-earth elements(HREE), with slightly negative Eu anomalies, and are enriched in Th, U and large ion lithophile elements(LILE, e.g., K and Rb), and depleted in high field strength elements(HFSE, e.g., Nb, Ta, P and Ti). They also show negative zircon εHf(t) values(-6.7 to -2.3) and negative whole rock εNd(t) values(à5.2 to-4.3), as well as old Hfe Nd model ages, indicating the magmas were derived from a thickened ancient lower crust within the garneteamphibolite facies. Considering the tectonic evolution of the Yidun Terrane, geochemical characteristics of granodiorite porphyry, and the ages of mineralization obtained in this study. We suggest that the Donglufang deposit was formed in a post-collisional setting, which has a genetic relationship with the emplacement of the granodiorite porphyry. The present study provide key information for the exploration of the Late Cretaceous metallogeny in the Yidun Terrane.展开更多
Background:The association and its population heterogeneities between low-density lipoprotein cholesterol(LDL-C)and all-cause and cardiovascular mortality remain unknown.We aimed to examine the dose-dependent associat...Background:The association and its population heterogeneities between low-density lipoprotein cholesterol(LDL-C)and all-cause and cardiovascular mortality remain unknown.We aimed to examine the dose-dependent associations of LDL-C levels with specific types of cardiovascular disease(CVD)mortality and heterogeneities in the associations among different population subgroups.Methods:A total of 2,968,462 participants aged 35-75 years from China Health Evaluation And risk Reduction through nationwide Teamwork(ChinaHEART)(2014-2019)were included.Cox proportional hazard models and Fine-Gray subdistribution hazard models were used to estimate associations between LDL-C categories(<70.0,70.0-99.9,100.0-129.9[reference group],130.0-159.9,160.0-189.9,and≥190.0 mg/dL)and all-cause and cause-specific mortality.Results:During a median follow-up of 3.7 years,57,391 and 23,241 deaths from all-cause and overall CVD were documented.We observed J-shaped associations between LDL-C and death from all-cause,overall CVD,coronary heart disease(CHD),and ischemic stroke,and an L-shaped association between LDL-C and hemorrhagic stroke(HS)mortality(P for non-linearity<0.001).Compared with the reference group(100.0-129.9 mg/dL),very low LDL-C levels(<70.0 mg/dL)were significantly associated with increased risk of overall CVD(hazard ratio[HR]:1.10,95%confidence interval[CI]:1.06-1.14)and HS mortality(HR:1.37,95%CI:1.29-1.45).Very high LDL-C levels(≥190.0 mg/dL)were associated with increased risk of overall CVD(HR:1.51,95%CI:1.40-1.62)and CHD mortality(HR:2.08,95%CI:1.92-2.24).The stronger associations of very low LDL-C with risk of CVD mortality were observed in individuals with older age,low or normal body mass index,low or moderate 10-year atherosclerotic CVD risk,and those without diagnosed CVD or taking statins.Stronger associations between very high LDL-C levels and all-cause and CVD mortality were observed in younger people.Conclusions:People with very low LDL-C had a higher risk of all-cause,CVD,and HS mortality;those with very high LDL-C had a higher risk of all-cause,CVD,and CHD mortality.On the basis of our findings,comprehensive health assessment is needed to evaluate cardiovascular risk and implement appropriate lipid-lowering therapy for people with very low LDL-C.展开更多
The effect of preparation parameters on the performance of zeolite for ammonium (20-300 mg N/L) adsorption from simulated wastewater is reported.It was found that the ratios of Na2O/SiO2 and Si/Al had a more important...The effect of preparation parameters on the performance of zeolite for ammonium (20-300 mg N/L) adsorption from simulated wastewater is reported.It was found that the ratios of Na2O/SiO2 and Si/Al had a more important influence than crystallization time on zeolite adsorption properties.Relatively low Na2O/SiO2 ratios were beneficial for fabrication of zeolites with high proportions of micropore area and volume,which led to the surface adsorption mechanism being dominated by surface free energy and pore effects.However,with decreasing Si/Al ratios,the effect of ion-exchange was more prominent due to the high negative surface potential of zeolite.In addition,the concentration of weak acid sites on the zeolites was increased with lower ratios of Na2O/SiO2 and Si/Al,which may promote ammonium removal.Therefore,the most effective zeolite for ammonium removal,which was fabricated at Na2O/SiO2 =1.375,Si/Al =4 and crystallization time of 48 hr,exhibited the cooperative effects of adsorption,ion-exchange and a large amount of weak acid sites.The maximum ammonium adsorption capacity (35.06 t 0.98 mg/g) and the removal efficiency (94.44%± 4.00%) were obtained at the dosage of 4.0 g/L zeolite NaX at ammonium concentrations of 300 mg N/L and 20 mg N/L,respectively.The Freundlich isotherm and pseudo-first-order kinetics models provided excellent fitting for the ammonium adsorption process.In addition,zeolite NaX showed about 1.23-3.2 times the ammonium adsorption capacity of clinoptilolite.The stable and efficient reusability of zeolite NaX after five regeneration cycles demonstrated that this adsorbent has considerable potential for practical industrial applications.展开更多
Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in ex...Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models.The OP9,a stromal cell line from mouse bone marrow,has hematopoietic supportive capacity.Here,we report that the OP9 has the immunophenotype （CD45-,CD11b-,FLK-1-,CD31-,CD34-,CD44＋,CD29＋,Sca-1＋,CD86-,and MHCII-） identical to canonical mouse MSCs.The expression of CD140a＋,CD140b＋,α-SMA＋ and Calponin＋ suggested the perivascular origin of OP9.Functionally,the OP9 had strong clonogenic ability and could be induced into osteocytes,chondrocytes and adipocytes.The lymphocyte transformation test （LTT） and mixed leukocyte reaction （MLR） showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens （PHA） or allogeneic lymphocytes （BALB/c T cells）.Finally,the migration of OP9 could be efficiently induced by bFGF,IGF-1,IL-3,PDGF-BB,TGF-β1 and TGF-β3.In conclusion,the OP9 were bona fide MSCs,and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.展开更多
Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stein cells （HSCs） are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos. ...Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stein cells （HSCs） are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos. This process is therefore known as the endothelial-to- hematopoietic transition （EHT）. Nevertheless, there is no functional evidence that documents the HSC transition from purified endothelial cells. In this study, we developed an OP9-DLl-based co-culture system that was able to facilitate the HSC specification and/or expansion in vitro of mouse embryonic day 10.5 （El0.5） Tie2~ cells remarkably. Then, the immunophenotypically defined endothelial ceils were harvested by a combination of surface markers （Flkl＋CD31 ~CD41 CD45 Ter119 ） from the caudal half of EI0.0-EI 1.0 mouse embryos. The transplantation of the endothelia/OP9-DL1 co-cultures led to long-term, high-level, multi-lineage, and multi-organ he- matopoietic reconstitution in the irradiated adult recipients. The induced HSC activity was initially observed at El0.5, and a significant increase was detected at El 1.0, which suggests a temporally specific regulation. Taken together, tbr the first time, we provide functional evidence showing the HSC potential of purified embryonic endothelial cells, which is indispensable for the emerging EHT concept. Moreover, the newly defined co-culture system will aid the exploration of the key molecules governing the HSC transition from embryonic and even postnatal endothelial cells, which has enormous significance in basic and translational research.展开更多
The objective of the study was to compare the concentration of lidocaine in different body fluids/tissues after subarachnoid space and intravenous administrations of a lethal dose of lidocaine.Totally 18 dogs were use...The objective of the study was to compare the concentration of lidocaine in different body fluids/tissues after subarachnoid space and intravenous administrations of a lethal dose of lidocaine.Totally 18 dogs were used in the experiment.Six dogs were given subarachnoid anesthesia,another were given an intravenous injection of a dose of 75 mg/kg weight of lidocaine hydrochloride in 5 min and the last 6 dogs were used as the blank control dogs and given a subarachnoid space injection or a femoral artery injection of the same volume of sodium chloride.As soon as its vital signs disappeared,each dog was dissected and the specimen,such as brain,cerebrospinal fluid(CSF)in lateral ventricle,CSF in subarachnoid space,spinal cord(cervical spinal cord,thoracic spinal cord,lumbar spinal cord,and waist spinal cord),heart,lung,liver,spleen,kidney,bile,urine,heart blood,peripheral blood,muscle in injection location,and muscle in no injection location,were collected for analysis of lidocaine immediately.Analysis was performed with gas chromatography‑mass spectrometry(GC‑MS).From the maximum to the minimum,the order of lidocaine concentration detected in the subarachnoid space‑administered dogs was as follows:CSF in subarachnoid space,waist spinal cord,thoracic spinal cord,CSF in lateral ventricle,lumbar spinal cord,cervical spinal cord,lung,kidney,muscle in injection location,heart,brain,spleen,heart blood,liver,peripheral blood,bile,muscle in no injection location,and urine.The order of lidocaine concentration detected in the intravenously administered dogs was as followed:Kidney,heart,lung,spleen,brain,liver,peripheral blood,bile,heart blood,cervical spinal cord,thoracic spinal cord,muscle in injection location,lumbar spinal cord,muscle in no injection location,CSF in subarachnoid space,urine,and CSF in lateral ventricle.The maximum concentration of lidocaine was detected in the subarachnoid space CSF of subarachnoid space‑administered dead dogs,while in intravenously injected dead dogs,the maximum concentration of lidocaine was detected in the kidney.Our study provides some useful data for the forensic identification of epidural anesthesia accidents to decide the way the lidocaine enters the body.展开更多
The health significance of triglyceride-rich lipoproteins,also known as remnant cholesterol,has been increasingly recognized.However,evidence of their associations with cause-specific mortality in the general populati...The health significance of triglyceride-rich lipoproteins,also known as remnant cholesterol,has been increasingly recognized.However,evidence of their associations with cause-specific mortality in the general population was previously insufficient.To explore these associations and their heterogeneities across subgroups,a prospective cohort study was conducted including 3,403,414 community-based participants from China HEART,an ongoing government-funded public health program throughout China,from November 2014 through December 2022.The study assessed mortality risk of all-cause mortality,cardiovascular disease(CVD)mortality(including mortality from ischemic heart diseases(IHD),ischemic stroke(IS),and hemorrhagic stroke(HS),separately),and cancer mortality(including lung cancer,stomach cancer,and liver cancer,separately).During the 4-year follow-up,23,646 individuals died from CVD(including 8807 from IHD,3067 from IS,and 5190 from HS),and 20,318 from cancer(including 6208 from lung cancer,3013 from liver cancer,and 2174 from stomach cancer).Compared with individuals with remnant cholesterol<17.9 mg/d L,multivariable-adjusted mortality hazard ratios(HRs)for individuals with remnant cholesterol≥27.7 mg/d L were 1.03(1.00–1.05)for all-cause mortality,1.17(1.12–1.21)for CVD(1.19(1.12–1.27)for IHD mortality,and 1.22(1.09–1.36)for IS mortality),and 0.90(0.87–0.94)for allcancer mortality(0.94(0.87–1.02)for lung cancer,0.59(0.53–0.66)for liver cancer,and 0.73(0.64–0.83)for stomach cancer).In summary,this study revealed a correlation between increased remnant cholesterol levels and an elevated risk of cardiovascular disease mortality,as well as a reduced risk of mortality for certain types of cancer.展开更多
Background Stroke is a devastating disease,including intracerebral haemorrhage(ICH)and ischaemic stroke.Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage.However,t...Background Stroke is a devastating disease,including intracerebral haemorrhage(ICH)and ischaemic stroke.Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage.However,the direct effects and features of systemic inflammation on brain injury,especially comparing between ischaemic and haemorrhagic stroke,are still obscure.Methods Pertussis toxin(PT)was used to build a pro-inflammatory milieu after ICH and ischaemic stroke in mouse model.The neurodeficits,stroke lesion,immune response and blood–brain barrier(BBB)destruction were assessed.Results In ICH mouse model,PT-induced systemic inflammation exacerbated neurological deficits,and enlarged haemorrhage lesion and perihaematomal oedema.We also found promoted leucocyte infiltration and inflammatory cytokine release into the brain after PT treatment.Moreover,the integrity of the BBB was further disrupted after receiving PT.Furthermore,we demonstrated that PT enhanced brain inflammation and aggravated stroke severity in middle cerebral artery occlusion mouse model.Conclusions Our results suggest that PT increases inflammatory response that exacerbates brain injury after ICH or ischaemic stroke in mouse model.展开更多
Stem cells(SCs)are cells with strong proliferation ability,multilineage differentiation potential and self-renewal capacity.SC transplantation represents an important therapeutic advancement for the treatment strategy...Stem cells(SCs)are cells with strong proliferation ability,multilineage differentiation potential and self-renewal capacity.SC transplantation represents an important therapeutic advancement for the treatment strategy of neurological diseases,both in the preclinical experimental and clinical settings.Innovative and breakthrough SC labelling and tracking technologies are widely used to monitor the distribution and viability of transplanted cells non-invasively and longitudinally.Here we summarised the research progress of the main tracers,labelling methods and imaging technologies involved in current SC tracking technologies for various neurological diseases.Finally,the applications,challenges and unresolved problems of current SC tracing technologies were discussed.展开更多
基金financially supported by the National Basic Research Program of China (No. 2015CB452605)the Natural Science Foundation of China (No. 41602089)Fundamental Research Funds for the Central Universities (No. 20170713174019)
文摘The newly-discovered Donglufang Moe Cu porphyry-skarn deposit is located in the southern Yidun Terrane, southeast Tibet, with more than 80 million tonnes(Mt) of reserves(grading 0.15 wt.% Mo and0.48 wt.% Cu) hosted in Triassic strata and Late Cretaceous granodiorite porphyry. Ree Os dating of molybdenum ore yielded a weighted mean age of 84.9 ± 1.0 Ma and an isochron age of 85.2 ± 0.6 Ma.LA-ICP-MS Ue Pb dating of zircons from the granodiorite porphyry yielded206 Pb/238 U ages ranging from 87.4 Ma to 84.2 Ma with a weighted mean206 Pb/238 U age of 85.1 ±0.5 Ma, indicating a temporal linkage between granitic magmatism and Moe Cu mineralization. Geochemical analyses show that the granodiorite porphyries are I-type granites with Si O_2 contents of 64.3 -66.7 wt.%. These rocks are typically metaluminous with high K_2 O/Na_2 O ratios, low Mg O(1.32 -1.56 wt.%), Cr(5.6 -12.9 ppm), Ni(3.79 -10.81 ppm), Mg#(43 -52) values, and high Sr(304 -844 ppm), Sr/Y(21.2 -50.8) and La/Yb ratios(37.0 -60.1). They are enriched in light rare-earth elements(LREE) relative to heavy rare-earth elements(HREE), with slightly negative Eu anomalies, and are enriched in Th, U and large ion lithophile elements(LILE, e.g., K and Rb), and depleted in high field strength elements(HFSE, e.g., Nb, Ta, P and Ti). They also show negative zircon εHf(t) values(-6.7 to -2.3) and negative whole rock εNd(t) values(à5.2 to-4.3), as well as old Hfe Nd model ages, indicating the magmas were derived from a thickened ancient lower crust within the garneteamphibolite facies. Considering the tectonic evolution of the Yidun Terrane, geochemical characteristics of granodiorite porphyry, and the ages of mineralization obtained in this study. We suggest that the Donglufang deposit was formed in a post-collisional setting, which has a genetic relationship with the emplacement of the granodiorite porphyry. The present study provide key information for the exploration of the Late Cretaceous metallogeny in the Yidun Terrane.
基金supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Science(No.2021-I2M-1-011)the National High Level Hospital Clinical Research Funding(Nos.2022-GSP-GG-4,2023-GSP-RC-20)the Ministry of Finance of China and National Health Commission of China,and the 111 Project from the Ministry of Education of China(No.B16005).
文摘Background:The association and its population heterogeneities between low-density lipoprotein cholesterol(LDL-C)and all-cause and cardiovascular mortality remain unknown.We aimed to examine the dose-dependent associations of LDL-C levels with specific types of cardiovascular disease(CVD)mortality and heterogeneities in the associations among different population subgroups.Methods:A total of 2,968,462 participants aged 35-75 years from China Health Evaluation And risk Reduction through nationwide Teamwork(ChinaHEART)(2014-2019)were included.Cox proportional hazard models and Fine-Gray subdistribution hazard models were used to estimate associations between LDL-C categories(<70.0,70.0-99.9,100.0-129.9[reference group],130.0-159.9,160.0-189.9,and≥190.0 mg/dL)and all-cause and cause-specific mortality.Results:During a median follow-up of 3.7 years,57,391 and 23,241 deaths from all-cause and overall CVD were documented.We observed J-shaped associations between LDL-C and death from all-cause,overall CVD,coronary heart disease(CHD),and ischemic stroke,and an L-shaped association between LDL-C and hemorrhagic stroke(HS)mortality(P for non-linearity<0.001).Compared with the reference group(100.0-129.9 mg/dL),very low LDL-C levels(<70.0 mg/dL)were significantly associated with increased risk of overall CVD(hazard ratio[HR]:1.10,95%confidence interval[CI]:1.06-1.14)and HS mortality(HR:1.37,95%CI:1.29-1.45).Very high LDL-C levels(≥190.0 mg/dL)were associated with increased risk of overall CVD(HR:1.51,95%CI:1.40-1.62)and CHD mortality(HR:2.08,95%CI:1.92-2.24).The stronger associations of very low LDL-C with risk of CVD mortality were observed in individuals with older age,low or normal body mass index,low or moderate 10-year atherosclerotic CVD risk,and those without diagnosed CVD or taking statins.Stronger associations between very high LDL-C levels and all-cause and CVD mortality were observed in younger people.Conclusions:People with very low LDL-C had a higher risk of all-cause,CVD,and HS mortality;those with very high LDL-C had a higher risk of all-cause,CVD,and CHD mortality.On the basis of our findings,comprehensive health assessment is needed to evaluate cardiovascular risk and implement appropriate lipid-lowering therapy for people with very low LDL-C.
基金supported by the China Postdoctoral Science Foundation(2018M631489)
文摘The effect of preparation parameters on the performance of zeolite for ammonium (20-300 mg N/L) adsorption from simulated wastewater is reported.It was found that the ratios of Na2O/SiO2 and Si/Al had a more important influence than crystallization time on zeolite adsorption properties.Relatively low Na2O/SiO2 ratios were beneficial for fabrication of zeolites with high proportions of micropore area and volume,which led to the surface adsorption mechanism being dominated by surface free energy and pore effects.However,with decreasing Si/Al ratios,the effect of ion-exchange was more prominent due to the high negative surface potential of zeolite.In addition,the concentration of weak acid sites on the zeolites was increased with lower ratios of Na2O/SiO2 and Si/Al,which may promote ammonium removal.Therefore,the most effective zeolite for ammonium removal,which was fabricated at Na2O/SiO2 =1.375,Si/Al =4 and crystallization time of 48 hr,exhibited the cooperative effects of adsorption,ion-exchange and a large amount of weak acid sites.The maximum ammonium adsorption capacity (35.06 t 0.98 mg/g) and the removal efficiency (94.44%± 4.00%) were obtained at the dosage of 4.0 g/L zeolite NaX at ammonium concentrations of 300 mg N/L and 20 mg N/L,respectively.The Freundlich isotherm and pseudo-first-order kinetics models provided excellent fitting for the ammonium adsorption process.In addition,zeolite NaX showed about 1.23-3.2 times the ammonium adsorption capacity of clinoptilolite.The stable and efficient reusability of zeolite NaX after five regeneration cycles demonstrated that this adsorbent has considerable potential for practical industrial applications.
基金supported by the National Natural Science Foundation (Nos 30871410 and 30600613)
文摘Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models.The OP9,a stromal cell line from mouse bone marrow,has hematopoietic supportive capacity.Here,we report that the OP9 has the immunophenotype （CD45-,CD11b-,FLK-1-,CD31-,CD34-,CD44＋,CD29＋,Sca-1＋,CD86-,and MHCII-） identical to canonical mouse MSCs.The expression of CD140a＋,CD140b＋,α-SMA＋ and Calponin＋ suggested the perivascular origin of OP9.Functionally,the OP9 had strong clonogenic ability and could be induced into osteocytes,chondrocytes and adipocytes.The lymphocyte transformation test （LTT） and mixed leukocyte reaction （MLR） showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens （PHA） or allogeneic lymphocytes （BALB/c T cells）.Finally,the migration of OP9 could be efficiently induced by bFGF,IGF-1,IL-3,PDGF-BB,TGF-β1 and TGF-β3.In conclusion,the OP9 were bona fide MSCs,and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
基金supported by the Chinese National Key Program on Basic Research(Nos.2011CB964800 and 2012CB966904)the National Natural Science Foundation of China(No. 30911130360)
文摘Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stein cells （HSCs） are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos. This process is therefore known as the endothelial-to- hematopoietic transition （EHT）. Nevertheless, there is no functional evidence that documents the HSC transition from purified endothelial cells. In this study, we developed an OP9-DLl-based co-culture system that was able to facilitate the HSC specification and/or expansion in vitro of mouse embryonic day 10.5 （El0.5） Tie2~ cells remarkably. Then, the immunophenotypically defined endothelial ceils were harvested by a combination of surface markers （Flkl＋CD31 ~CD41 CD45 Ter119 ） from the caudal half of EI0.0-EI 1.0 mouse embryos. The transplantation of the endothelia/OP9-DL1 co-cultures led to long-term, high-level, multi-lineage, and multi-organ he- matopoietic reconstitution in the irradiated adult recipients. The induced HSC activity was initially observed at El0.5, and a significant increase was detected at El 1.0, which suggests a temporally specific regulation. Taken together, tbr the first time, we provide functional evidence showing the HSC potential of purified embryonic endothelial cells, which is indispensable for the emerging EHT concept. Moreover, the newly defined co-culture system will aid the exploration of the key molecules governing the HSC transition from embryonic and even postnatal endothelial cells, which has enormous significance in basic and translational research.
基金funded by the National Natural Science Foundation Council of China(No.81172906)the National Key Technology R&D Program of China(No.2012BAK02B02-2)International technology cooperation plan project in Shanxi Province(No.2012081053).
文摘The objective of the study was to compare the concentration of lidocaine in different body fluids/tissues after subarachnoid space and intravenous administrations of a lethal dose of lidocaine.Totally 18 dogs were used in the experiment.Six dogs were given subarachnoid anesthesia,another were given an intravenous injection of a dose of 75 mg/kg weight of lidocaine hydrochloride in 5 min and the last 6 dogs were used as the blank control dogs and given a subarachnoid space injection or a femoral artery injection of the same volume of sodium chloride.As soon as its vital signs disappeared,each dog was dissected and the specimen,such as brain,cerebrospinal fluid(CSF)in lateral ventricle,CSF in subarachnoid space,spinal cord(cervical spinal cord,thoracic spinal cord,lumbar spinal cord,and waist spinal cord),heart,lung,liver,spleen,kidney,bile,urine,heart blood,peripheral blood,muscle in injection location,and muscle in no injection location,were collected for analysis of lidocaine immediately.Analysis was performed with gas chromatography‑mass spectrometry(GC‑MS).From the maximum to the minimum,the order of lidocaine concentration detected in the subarachnoid space‑administered dogs was as follows:CSF in subarachnoid space,waist spinal cord,thoracic spinal cord,CSF in lateral ventricle,lumbar spinal cord,cervical spinal cord,lung,kidney,muscle in injection location,heart,brain,spleen,heart blood,liver,peripheral blood,bile,muscle in no injection location,and urine.The order of lidocaine concentration detected in the intravenously administered dogs was as followed:Kidney,heart,lung,spleen,brain,liver,peripheral blood,bile,heart blood,cervical spinal cord,thoracic spinal cord,muscle in injection location,lumbar spinal cord,muscle in no injection location,CSF in subarachnoid space,urine,and CSF in lateral ventricle.The maximum concentration of lidocaine was detected in the subarachnoid space CSF of subarachnoid space‑administered dead dogs,while in intravenously injected dead dogs,the maximum concentration of lidocaine was detected in the kidney.Our study provides some useful data for the forensic identification of epidural anesthesia accidents to decide the way the lidocaine enters the body.
基金supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Science(2021-1-I2M-011)the National High-Level Hospital Clinical Research Funding(2022-GSP-GG-4)+1 种基金the Ministry of Finance of China and National Health Commission of Chinathe 111 Project from the Ministry of Education of China(B16005).
文摘The health significance of triglyceride-rich lipoproteins,also known as remnant cholesterol,has been increasingly recognized.However,evidence of their associations with cause-specific mortality in the general population was previously insufficient.To explore these associations and their heterogeneities across subgroups,a prospective cohort study was conducted including 3,403,414 community-based participants from China HEART,an ongoing government-funded public health program throughout China,from November 2014 through December 2022.The study assessed mortality risk of all-cause mortality,cardiovascular disease(CVD)mortality(including mortality from ischemic heart diseases(IHD),ischemic stroke(IS),and hemorrhagic stroke(HS),separately),and cancer mortality(including lung cancer,stomach cancer,and liver cancer,separately).During the 4-year follow-up,23,646 individuals died from CVD(including 8807 from IHD,3067 from IS,and 5190 from HS),and 20,318 from cancer(including 6208 from lung cancer,3013 from liver cancer,and 2174 from stomach cancer).Compared with individuals with remnant cholesterol<17.9 mg/d L,multivariable-adjusted mortality hazard ratios(HRs)for individuals with remnant cholesterol≥27.7 mg/d L were 1.03(1.00–1.05)for all-cause mortality,1.17(1.12–1.21)for CVD(1.19(1.12–1.27)for IHD mortality,and 1.22(1.09–1.36)for IS mortality),and 0.90(0.87–0.94)for allcancer mortality(0.94(0.87–1.02)for lung cancer,0.59(0.53–0.66)for liver cancer,and 0.73(0.64–0.83)for stomach cancer).In summary,this study revealed a correlation between increased remnant cholesterol levels and an elevated risk of cardiovascular disease mortality,as well as a reduced risk of mortality for certain types of cancer.
基金supported in part by the National Science Foundation of China(81971094,81771274)the Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing,China.
文摘Background Stroke is a devastating disease,including intracerebral haemorrhage(ICH)and ischaemic stroke.Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage.However,the direct effects and features of systemic inflammation on brain injury,especially comparing between ischaemic and haemorrhagic stroke,are still obscure.Methods Pertussis toxin(PT)was used to build a pro-inflammatory milieu after ICH and ischaemic stroke in mouse model.The neurodeficits,stroke lesion,immune response and blood–brain barrier(BBB)destruction were assessed.Results In ICH mouse model,PT-induced systemic inflammation exacerbated neurological deficits,and enlarged haemorrhage lesion and perihaematomal oedema.We also found promoted leucocyte infiltration and inflammatory cytokine release into the brain after PT treatment.Moreover,the integrity of the BBB was further disrupted after receiving PT.Furthermore,we demonstrated that PT enhanced brain inflammation and aggravated stroke severity in middle cerebral artery occlusion mouse model.Conclusions Our results suggest that PT increases inflammatory response that exacerbates brain injury after ICH or ischaemic stroke in mouse model.
基金by National Science Foundation of China(81801199,81830038,81971094,91949208)Beijing Municipal Science&Technology Commission(Z181100001818001).
文摘Stem cells(SCs)are cells with strong proliferation ability,multilineage differentiation potential and self-renewal capacity.SC transplantation represents an important therapeutic advancement for the treatment strategy of neurological diseases,both in the preclinical experimental and clinical settings.Innovative and breakthrough SC labelling and tracking technologies are widely used to monitor the distribution and viability of transplanted cells non-invasively and longitudinally.Here we summarised the research progress of the main tracers,labelling methods and imaging technologies involved in current SC tracking technologies for various neurological diseases.Finally,the applications,challenges and unresolved problems of current SC tracing technologies were discussed.
