Objective: This study aims to elucidate and quantify the composition of Jiangtang Qingre formula(JQF), delineate the absorbed components in the bloodstream, predict the major biologically active components, and identi...Objective: This study aims to elucidate and quantify the composition of Jiangtang Qingre formula(JQF), delineate the absorbed components in the bloodstream, predict the major biologically active components, and identify potential targets for the treatment of diabetes mellitus(DM). Materials and Methods: The chemical composition and metabolites of JQF were elucidated using ultra-high-performance liquid chromatography(UHPLC)-linear ion trap quadrupole-orbitrap high-resolution mass spectrometry(MS). The various components of JQF were concurrently determined using UHPLC-triple–quadrupole MS. Network pharmacological analysis was employed to explore the bioactive components and potential therapeutic targets in DM. Results: A total of 63 compounds were identified and provisionally characterized, with fiavones, organic acids, and alkaloids emerging as the major chemical constituents. A robust analytical method that enables the simultaneous quantification of 24 representative components was successfully developed. The contents of 11 batches of samples were assessed. Ten prototype components were identified in rat plasma. The pathways associated with the efficacy of JQF in DM treatment were linked to signal transduction, endocrine and immune systems, lipid metabolism, and amino acid metabolism. Conclusion: This study systematically and comprehensively characterized the major chemical components and patterns in JQF, laying the groundwork for understanding its pharmacodynamic mechanisms and clinical applications.展开更多
基金financially by the Scientific and technological innovation project of China Academy of Chinese Medical Sciences (No. CI2021A01606, CI2021A04507, CI2021A04510, Cl2021A04502, CI2021A04509)National Administration of Traditional Chinese Medicine Young Qihuang Scholar Project,ChinaQianjiang Distinguished Expert Project of Hangzhou (2020), China
文摘Objective: This study aims to elucidate and quantify the composition of Jiangtang Qingre formula(JQF), delineate the absorbed components in the bloodstream, predict the major biologically active components, and identify potential targets for the treatment of diabetes mellitus(DM). Materials and Methods: The chemical composition and metabolites of JQF were elucidated using ultra-high-performance liquid chromatography(UHPLC)-linear ion trap quadrupole-orbitrap high-resolution mass spectrometry(MS). The various components of JQF were concurrently determined using UHPLC-triple–quadrupole MS. Network pharmacological analysis was employed to explore the bioactive components and potential therapeutic targets in DM. Results: A total of 63 compounds were identified and provisionally characterized, with fiavones, organic acids, and alkaloids emerging as the major chemical constituents. A robust analytical method that enables the simultaneous quantification of 24 representative components was successfully developed. The contents of 11 batches of samples were assessed. Ten prototype components were identified in rat plasma. The pathways associated with the efficacy of JQF in DM treatment were linked to signal transduction, endocrine and immune systems, lipid metabolism, and amino acid metabolism. Conclusion: This study systematically and comprehensively characterized the major chemical components and patterns in JQF, laying the groundwork for understanding its pharmacodynamic mechanisms and clinical applications.
