Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal ex...Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.展开更多
To treat technical problems of data acquisition,alarm management, and historical data archiving of radiation protection control system in Thorium Molten Salt Reactor, a network prototype system was designed based on e...To treat technical problems of data acquisition,alarm management, and historical data archiving of radiation protection control system in Thorium Molten Salt Reactor, a network prototype system was designed based on experimental physics and industrial control system.Radiation level of many locations in Jiading campus of Shanghai Institute of Applied Physics is being continuously monitored, and boundaries of accelerator facilities and radiochemical areas are defined by the access control system. In this paper, we introduce the control system design,including human–machine interfaces, alarm system, historical data archiving system, and the software for access control. The software development followed the standard of Capability Maturity Model Integration(CMMIò) Level3, and the software had passed a third-party test, which indicated that the functionality and the reliability could fulfill the requirements of the radiation protection system.展开更多
Heart disease is a major cause of human death worldwide.The switching/sucrose non-fermenting(SWI/SNF)complex plays a crucial role during heart development and heart disease.Brahma-related gene 1(Brg1),a critical adeno...Heart disease is a major cause of human death worldwide.The switching/sucrose non-fermenting(SWI/SNF)complex plays a crucial role during heart development and heart disease.Brahma-related gene 1(Brg1),a critical adenosine triphosphatase(ATP)catalytic subunit of the SWI/SNF complex,participates in several physiological and pathological processes by utilizing the energy generated by ATP hydrolysis to regulate gene transcription,DNA repair,and DNA replication.And Brg1 is involved in systemic diseases,including nervous,cardiovascular,and immune system diseases.Recently,the role of Brg1 in heart disease has been studied in-depth.展开更多
基金funding from the Basic Research Project of the Education Department of Shaanxi Province(21JC010,21JP035)the Young and Middle-Aged Scientific Research and Innovation Team of the Shaanxi Provincial Administration of Traditional Chinese Medicine(2022SLRHLJ001)the 2023 Central Financial Transfer Payment Local Project“Innovation and Improvement of Five Types of Hospital Preparations,Such as Roumudan Granules”.
文摘Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.
基金supported by the ‘‘Strategic Priority Research Program’’ of the Chinese Academy of Sciences (No. XDA02010300)
文摘To treat technical problems of data acquisition,alarm management, and historical data archiving of radiation protection control system in Thorium Molten Salt Reactor, a network prototype system was designed based on experimental physics and industrial control system.Radiation level of many locations in Jiading campus of Shanghai Institute of Applied Physics is being continuously monitored, and boundaries of accelerator facilities and radiochemical areas are defined by the access control system. In this paper, we introduce the control system design,including human–machine interfaces, alarm system, historical data archiving system, and the software for access control. The software development followed the standard of Capability Maturity Model Integration(CMMIò) Level3, and the software had passed a third-party test, which indicated that the functionality and the reliability could fulfill the requirements of the radiation protection system.
文摘Heart disease is a major cause of human death worldwide.The switching/sucrose non-fermenting(SWI/SNF)complex plays a crucial role during heart development and heart disease.Brahma-related gene 1(Brg1),a critical adenosine triphosphatase(ATP)catalytic subunit of the SWI/SNF complex,participates in several physiological and pathological processes by utilizing the energy generated by ATP hydrolysis to regulate gene transcription,DNA repair,and DNA replication.And Brg1 is involved in systemic diseases,including nervous,cardiovascular,and immune system diseases.Recently,the role of Brg1 in heart disease has been studied in-depth.
