AIM:To evaluate the outcomes of patients withmedium-sized hepatocellular carcinoma(HCC)who underwent percutaneous microwave ablation(MWA).METHODS:We retrospectively reviewed all patients with a single medium-sized HCC...AIM:To evaluate the outcomes of patients withmedium-sized hepatocellular carcinoma(HCC)who underwent percutaneous microwave ablation(MWA).METHODS:We retrospectively reviewed all patients with a single medium-sized HCC who underwent percutaneous MWA from January 2010 to January2013.Technical success,technical effectiveness and complications were subsequently observed.Survival curves were constructed using the Kaplan-Meier method.The Cox proportional hazards model was fitted to each variable.The relative prognostic significance o f t h e va r i a b l e s fo r p re d i c t i n g ove ra l l s u r v i va l rate,recurrence-free survival rate and local tumor recurrence(s)was assessed using univariate analysis.All variables with a P value<0.20 were subjected to multivariate analysis.RESULTS:The study included 182 patients(mean age,58 years;age range:22-86 years)with a single HCC(mean size,3.72±0.54 cm;range:3.02-5.00cm).The estimated technical effectiveness rate was93%in 182 patients.The major complication rate was2.7%(5/182),including liver abscess in 4 cases,and abdominal bleeding at the puncture site in 1 case.Thirty-day mortality rate was 0.5%(1/182).One patient died due to liver abscess-related septicemia.Cumulative recurrence-free survival and overall survival(OS)rates were 51%,36%,27%and 89%,74%,60%at 1,2,and 3 years,respectively.Age(P=0.017)and tumor diameter(P=0.029)were independent factors associated with local tumor recurrence.None of the factors had a statistically significant impact on recurrence-free survival.Serum albumin level(P=0.009)and new lesion(s)(P=0.029)were independently associated with OS.CONCLUSION:Percutaneous MWA is a relatively safe and effective treatment for patients with medium-sized HCC.展开更多
AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive...AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive and Liver Disease Department of our hospital were randomly selected. RT-PCR was used to detect the level of miR-106b in serum samples. Laboratory and imaging examinations were carried out to confirm the HCC diagnosis in patients who had a > 2-fold change in miR-106b levels. Ultrasound-guided biopsy was also used for HCC diagnosis when necessary. On this basis, the clinical data of these subjects, including history of hepatitis virus infection, obesity, long-term history of alcohol use and stage of HCC, were collected. Then, the impact of these factors on the level of miR1-06b in serum was analyzed. Furthermore, receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic efficacy of miR-106b for HCC. RESULTS A total of 35 subjects had abnormal serum miR-106b levels, of which 20 subjects were diagnosed with HCC. t-test revealed that the difference in serum miR-106b level in terms of sex, age, history of hepatitis virus infection, obesity and long-term history of alcohol use was not statistically significant. However, serum miR-106b levels in patients with advanced HCC (stage. /.) was higher than in patients with early HCC (stage./.), and the difference was statistically significant (P = 0.000). Moreover, the ROC curve revealed that the area under the curve value for miR-106b was 0.885, which shows that serum miR-106b level has a certain clinical value for HCC diagnosis. CONCLUSION The random sampling survey shows that serum miR-106b level is a valuable diagnostic marker for HCC. However, the diagnostic threshold value needs to be further researched.展开更多
The polymerase chain reaction(PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three...The polymerase chain reaction(PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv.oryzae, X. oryzae pv. oryzicola, and Burkholderia glumae. The unique PCR primer sets were designed from portions of a putative glycosyltransferase gene of X. oryzae pv. oryzae, an Avr Rxo gene of X. oryzae pv. oryzicola, and an internal transcribed spacer(ITS) sequence of B. glumae. Using a multiplex PCR assay, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix. Using SYBR Green real-time PCR assays, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected at 1, 1, and 10 fg μL-1, respectively. These newly designed molecular assays are sensitive and could be reliable tools for pathogen detection and disease forecasting.展开更多
Catalytic performances of the CO2 reforming of toluene on Co/MgO catalysts with different cobalt loadings were evaluated in a fluidized-bed reactor. The results showed that the conversion of toluene and the stability ...Catalytic performances of the CO2 reforming of toluene on Co/MgO catalysts with different cobalt loadings were evaluated in a fluidized-bed reactor. The results showed that the conversion of toluene and the stability of Co/MgO increased, but the apparent reaction rate decreased at the initial stage with increasing the amount of metallic Co formed from the reduction of Co/MgO catalysts at 700 ~C. The deactivation of Co/MgO catalysts was mainly resulted from that a part of the metallic Co was oxidized by CO2 and could not be re-reduced by H2 at reaction temperature. Therefore, the excess metallic Co on the higher Co loading catalysts was beneficial to the catalyst stability.展开更多
BACKGROUND Non-alcoholic fatty liver disease has become the most common chronic liver disease worldwide,which originates from the accumulation of triglyceride(TG)in the liver.Patients with type 2 diabetes mellitus(T2D...BACKGROUND Non-alcoholic fatty liver disease has become the most common chronic liver disease worldwide,which originates from the accumulation of triglyceride(TG)in the liver.Patients with type 2 diabetes mellitus(T2DM)are considered to have a predisposition to hepatic steatosis.However,the influencing factors for hepatic fat accumulation in T2DM patients remain unclear.AIM To investigate the influencing factors for hepatic fat accumulation in T2DM patients.METHODS We enrolled 329 T2DM patients admitted to the Endocrinology Department of the First Affiliated Hospital of Soochow University,who underwent MR mDIXONQuant examination to quantify the hepatic fat fraction(HFF).According to body mass index(BMI),the patients were divided into normal weight,overweight,and obese groups.The differences in general statistics,biochemical parameters,islet function,and HFF were compared among the three groups.The associations between HFF and other parameters and the influences of various parameters on the severity of hepatic fat accumulation were analyzed.RESULTS The HFF of T2DM patients gradually increased in the normal weight,overweight,and obese groups(P<0.05).Spearman correlation analysis showed that in T2DM patients,HFF was negatively correlated with age and high-density lipoprotein cholesterol(P<0.05),whereas it was positively correlated with BMI,waist-hip ratio,fasting plasma glucose,alanine aminotransferase(ALT),aspartate aminotransferase,bilirubin,glutamyl transpeptidase,lactate dehydrogenase,albumin(ALB),uric acid(UA),total cholesterol,TG,low-density lipoprotein cholesterol(LDL-C),C-reactive protein,free triiodothyronine,fasting insulin,fasting C-peptide,and homeostasis model assessment of insulin resistance(P<0.05).Multiple linear regression analysis showed significant positive influences of BMI,ALT,LDL-C,UA,and ALB on HFF in T2DM patients(P<0.05).Binary logistic regression analysis showed that BMI,ALT,ALB,and LDL-C were independent risk factors for moderate to severe fatty liver in T2DM patients,and obesity increased the risk of being complicated with moderate to severe fatty liver by 4.03 times(P<0.05).CONCLUSION The HFF of T2DM patients increases with BMI.Higher BMI,ALT,ALB,and LDLC are independent risk factors for moderate to severe fatty liver in T2DM patients.展开更多
文摘AIM:To evaluate the outcomes of patients withmedium-sized hepatocellular carcinoma(HCC)who underwent percutaneous microwave ablation(MWA).METHODS:We retrospectively reviewed all patients with a single medium-sized HCC who underwent percutaneous MWA from January 2010 to January2013.Technical success,technical effectiveness and complications were subsequently observed.Survival curves were constructed using the Kaplan-Meier method.The Cox proportional hazards model was fitted to each variable.The relative prognostic significance o f t h e va r i a b l e s fo r p re d i c t i n g ove ra l l s u r v i va l rate,recurrence-free survival rate and local tumor recurrence(s)was assessed using univariate analysis.All variables with a P value<0.20 were subjected to multivariate analysis.RESULTS:The study included 182 patients(mean age,58 years;age range:22-86 years)with a single HCC(mean size,3.72±0.54 cm;range:3.02-5.00cm).The estimated technical effectiveness rate was93%in 182 patients.The major complication rate was2.7%(5/182),including liver abscess in 4 cases,and abdominal bleeding at the puncture site in 1 case.Thirty-day mortality rate was 0.5%(1/182).One patient died due to liver abscess-related septicemia.Cumulative recurrence-free survival and overall survival(OS)rates were 51%,36%,27%and 89%,74%,60%at 1,2,and 3 years,respectively.Age(P=0.017)and tumor diameter(P=0.029)were independent factors associated with local tumor recurrence.None of the factors had a statistically significant impact on recurrence-free survival.Serum albumin level(P=0.009)and new lesion(s)(P=0.029)were independently associated with OS.CONCLUSION:Percutaneous MWA is a relatively safe and effective treatment for patients with medium-sized HCC.
文摘AIM To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels. METHODS A total of 335 subjects who underwent checkups in the Digestive and Liver Disease Department of our hospital were randomly selected. RT-PCR was used to detect the level of miR-106b in serum samples. Laboratory and imaging examinations were carried out to confirm the HCC diagnosis in patients who had a > 2-fold change in miR-106b levels. Ultrasound-guided biopsy was also used for HCC diagnosis when necessary. On this basis, the clinical data of these subjects, including history of hepatitis virus infection, obesity, long-term history of alcohol use and stage of HCC, were collected. Then, the impact of these factors on the level of miR1-06b in serum was analyzed. Furthermore, receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic efficacy of miR-106b for HCC. RESULTS A total of 35 subjects had abnormal serum miR-106b levels, of which 20 subjects were diagnosed with HCC. t-test revealed that the difference in serum miR-106b level in terms of sex, age, history of hepatitis virus infection, obesity and long-term history of alcohol use was not statistically significant. However, serum miR-106b levels in patients with advanced HCC (stage. /.) was higher than in patients with early HCC (stage./.), and the difference was statistically significant (P = 0.000). Moreover, the ROC curve revealed that the area under the curve value for miR-106b was 0.885, which shows that serum miR-106b level has a certain clinical value for HCC diagnosis. CONCLUSION The random sampling survey shows that serum miR-106b level is a valuable diagnostic marker for HCC. However, the diagnostic threshold value needs to be further researched.
基金support of the National 863 Project (2012AA021601)the New Seedling program for graduate students of Zhejiang Province (2012R409012)
文摘The polymerase chain reaction(PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv.oryzae, X. oryzae pv. oryzicola, and Burkholderia glumae. The unique PCR primer sets were designed from portions of a putative glycosyltransferase gene of X. oryzae pv. oryzae, an Avr Rxo gene of X. oryzae pv. oryzicola, and an internal transcribed spacer(ITS) sequence of B. glumae. Using a multiplex PCR assay, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix. Using SYBR Green real-time PCR assays, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected at 1, 1, and 10 fg μL-1, respectively. These newly designed molecular assays are sensitive and could be reliable tools for pathogen detection and disease forecasting.
基金supported by the National Basic Research Program of China(2013CB228104)the Hi-Tech Research and Development Program of China(2007AA05Z415)
文摘Catalytic performances of the CO2 reforming of toluene on Co/MgO catalysts with different cobalt loadings were evaluated in a fluidized-bed reactor. The results showed that the conversion of toluene and the stability of Co/MgO increased, but the apparent reaction rate decreased at the initial stage with increasing the amount of metallic Co formed from the reduction of Co/MgO catalysts at 700 ~C. The deactivation of Co/MgO catalysts was mainly resulted from that a part of the metallic Co was oxidized by CO2 and could not be re-reduced by H2 at reaction temperature. Therefore, the excess metallic Co on the higher Co loading catalysts was beneficial to the catalyst stability.
基金Suzhou Health Committee and Science and Technology Bureau,No.KJXW2019005.
文摘BACKGROUND Non-alcoholic fatty liver disease has become the most common chronic liver disease worldwide,which originates from the accumulation of triglyceride(TG)in the liver.Patients with type 2 diabetes mellitus(T2DM)are considered to have a predisposition to hepatic steatosis.However,the influencing factors for hepatic fat accumulation in T2DM patients remain unclear.AIM To investigate the influencing factors for hepatic fat accumulation in T2DM patients.METHODS We enrolled 329 T2DM patients admitted to the Endocrinology Department of the First Affiliated Hospital of Soochow University,who underwent MR mDIXONQuant examination to quantify the hepatic fat fraction(HFF).According to body mass index(BMI),the patients were divided into normal weight,overweight,and obese groups.The differences in general statistics,biochemical parameters,islet function,and HFF were compared among the three groups.The associations between HFF and other parameters and the influences of various parameters on the severity of hepatic fat accumulation were analyzed.RESULTS The HFF of T2DM patients gradually increased in the normal weight,overweight,and obese groups(P<0.05).Spearman correlation analysis showed that in T2DM patients,HFF was negatively correlated with age and high-density lipoprotein cholesterol(P<0.05),whereas it was positively correlated with BMI,waist-hip ratio,fasting plasma glucose,alanine aminotransferase(ALT),aspartate aminotransferase,bilirubin,glutamyl transpeptidase,lactate dehydrogenase,albumin(ALB),uric acid(UA),total cholesterol,TG,low-density lipoprotein cholesterol(LDL-C),C-reactive protein,free triiodothyronine,fasting insulin,fasting C-peptide,and homeostasis model assessment of insulin resistance(P<0.05).Multiple linear regression analysis showed significant positive influences of BMI,ALT,LDL-C,UA,and ALB on HFF in T2DM patients(P<0.05).Binary logistic regression analysis showed that BMI,ALT,ALB,and LDL-C were independent risk factors for moderate to severe fatty liver in T2DM patients,and obesity increased the risk of being complicated with moderate to severe fatty liver by 4.03 times(P<0.05).CONCLUSION The HFF of T2DM patients increases with BMI.Higher BMI,ALT,ALB,and LDLC are independent risk factors for moderate to severe fatty liver in T2DM patients.
