Seed storage proteins and their abundance are closely related to the formation of rice quality and grain size.A better understanding of the molecular basis of seed storage proteins will provide important information f...Seed storage proteins and their abundance are closely related to the formation of rice quality and grain size.A better understanding of the molecular basis of seed storage proteins will provide important information for developing new rice breeding strategies.In this study,we report that a seed storage protein albumin,named OsRAL5,positively regulates rice quality and grain size.OsRAL5 is specifically expressed during the grain-filling stage,suggesting its key role in regulating seed development.Gene editing of OsRAL5 using the CRISPR/Cas9 system diminished grain size and weight,resulting in the development of white-core endosperm and a reduction in eating and cooking quality(ECQ).Conversely,the endosperm appearance became transparent,and both grain weight and ECQ improved in the OsRAL5 over-expressed lines.The major seed storage components differed significantly between the OsRAL5-edited(dOsRAL5-TS)and OsRAL5-overexpressed(OsRAL5-OE)lines compared with the wild type.The protein and total lipid contents both decreased in the dOsRAL5-TS lines and increased in the OsRAL5-OE lines.Collectively,the over-expression of OsRAL5 significantly increased nutritional content and simultaneously improved yield and ECQ.These results imply that OsRAL5 might be a promising candidate gene for breeding super rice varieties with increased yield potential and superior quality.展开更多
目的调查ICU谵妄的严重程度,分析其影响因素,探讨对患者预后的影响。方法纳入2022年9月一2023年9月入住山西省太原市某三级甲等医院综合ICU的患者410例,将其分为亚谵妄组、轻中度谵妄组和重度谵妄组,收集相关资料并于出院90d后随访。采...目的调查ICU谵妄的严重程度,分析其影响因素,探讨对患者预后的影响。方法纳入2022年9月一2023年9月入住山西省太原市某三级甲等医院综合ICU的患者410例,将其分为亚谵妄组、轻中度谵妄组和重度谵妄组,收集相关资料并于出院90d后随访。采用多因素Logistic回归分析谵妄严重程度的影响因素,采用Kaplan-Meier法绘制患者出院后90d的累积生存曲线并进行Log-rank检验。结果ICU谵妄发生率为36.77%;谵妄严重程度占比中,亚谵妄占32.20%,轻中度谵妄占47.32%,重度谵妄占20.48%。以亚妄为参照,年龄是轻中度谵妄的危险因素;机械通气时间是重度谵妄的危险因素;苯二氮草类药物使用、降钙素原、急性生理与慢性健康评分Ⅱ(Acute Physiology And Chronic Health EvaluationⅡ,APACHEⅡ)、胃肠功能分级是谵妄严重程度的危险因素(P<0.05);不同谵妄严重程度患者出院后90d病死率有影响。展开更多
基金supported by the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20211137)China Agriculture Research System(Grant No.CARS-01)。
文摘Seed storage proteins and their abundance are closely related to the formation of rice quality and grain size.A better understanding of the molecular basis of seed storage proteins will provide important information for developing new rice breeding strategies.In this study,we report that a seed storage protein albumin,named OsRAL5,positively regulates rice quality and grain size.OsRAL5 is specifically expressed during the grain-filling stage,suggesting its key role in regulating seed development.Gene editing of OsRAL5 using the CRISPR/Cas9 system diminished grain size and weight,resulting in the development of white-core endosperm and a reduction in eating and cooking quality(ECQ).Conversely,the endosperm appearance became transparent,and both grain weight and ECQ improved in the OsRAL5 over-expressed lines.The major seed storage components differed significantly between the OsRAL5-edited(dOsRAL5-TS)and OsRAL5-overexpressed(OsRAL5-OE)lines compared with the wild type.The protein and total lipid contents both decreased in the dOsRAL5-TS lines and increased in the OsRAL5-OE lines.Collectively,the over-expression of OsRAL5 significantly increased nutritional content and simultaneously improved yield and ECQ.These results imply that OsRAL5 might be a promising candidate gene for breeding super rice varieties with increased yield potential and superior quality.
文摘目的调查ICU谵妄的严重程度,分析其影响因素,探讨对患者预后的影响。方法纳入2022年9月一2023年9月入住山西省太原市某三级甲等医院综合ICU的患者410例,将其分为亚谵妄组、轻中度谵妄组和重度谵妄组,收集相关资料并于出院90d后随访。采用多因素Logistic回归分析谵妄严重程度的影响因素,采用Kaplan-Meier法绘制患者出院后90d的累积生存曲线并进行Log-rank检验。结果ICU谵妄发生率为36.77%;谵妄严重程度占比中,亚谵妄占32.20%,轻中度谵妄占47.32%,重度谵妄占20.48%。以亚妄为参照,年龄是轻中度谵妄的危险因素;机械通气时间是重度谵妄的危险因素;苯二氮草类药物使用、降钙素原、急性生理与慢性健康评分Ⅱ(Acute Physiology And Chronic Health EvaluationⅡ,APACHEⅡ)、胃肠功能分级是谵妄严重程度的危险因素(P<0.05);不同谵妄严重程度患者出院后90d病死率有影响。
