An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascert...An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascertain at early infection stages owing to the unfavourable transcript representation compared to the host genes.In this study,we compare two sequencing techniques,RNAseq and enrichment sequencing(RenSeq and PenSeq)of cDNA,to investigate gene expression patterns in the doubled monoploid potato(DM)infected with the late blight pathogen Phytophthora infestans.Our results reveal distinct advantages of cDNA RenSeq and PenSeq over traditional RNAseq in terms of target gene representation and transcriptional quantification at early infection stages.Throughout the infection time course,cDNA enrichment sequencing enables transcriptomic analyses for more targeted host and pathogen genes.For highly expressed genes that were sampled in parallel by both cDNA enrichment and RNAseq,a high level of concordance in expression profiles is observed,indicative of at least semi-quantitative gene expression representation following enrichment.展开更多
A 45-year-old female with known situs inversus totalis presented with left-sided abdominal discomfort. Chest X-ray, abdominal ultrasonography and CT scan confirmed the diagnosis of a gallstone, as well as, situs inver...A 45-year-old female with known situs inversus totalis presented with left-sided abdominal discomfort. Chest X-ray, abdominal ultrasonography and CT scan confirmed the diagnosis of a gallstone, as well as, situs inversus;Laparoscopic cholecystectomy was safely performed with mirror image of standard 4 ports. Callots triangle dissection was done with epigastric working port by surgeon, but gallbladder fossa dissection was done by surgical assistant from midclavicular port as main working port. Laparoscopic surgeon should be careful for view of reversed relationships and also for existence of other anomalies.展开更多
The expression of estrogen receptor(ER)and human epidermal growth factor receptor-2(HER2)in breast cancer can change in response to treatment and pivotally influence tumor behavior and clinical management[1].Receptor ...The expression of estrogen receptor(ER)and human epidermal growth factor receptor-2(HER2)in breast cancer can change in response to treatment and pivotally influence tumor behavior and clinical management[1].Receptor discordance has been observed at various distant metastatic site(bone,lung,liver,and brain),with a routine loss of ER and gains in HER2 reported[2].This receptor discordance can influence tumor responsiveness to both HER2 inhibitor and endocrine therapies.Although the mechanisms underlying receptor expression changes are not fully understood,we recently reported gains in ESR1 promoter hypermethylation as a potential driver of ER loss during disease progression[3].In this study,mechanisms underlying altered receptor expression and associated disease outcomes were examined following neoadjuvant trastuzumab treatment.展开更多
In this study we have reported the design and development of a facile,sensitive,selective,and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers.Electrospu...In this study we have reported the design and development of a facile,sensitive,selective,and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers.Electrospun nanofibers were characterized using scanning electron microscope(SEM),transmission electron microscope(TEM),X-ray diffraction(XRD),X-ray photoelectron spectroscope(XPS),UV-Visible spectroscope(UV-VIS),and Fourier-transform infrared spectroscope(FTIR).Electrochemical properties of MWCNT-ZnO nanofiber-modified electrodes were assessed using electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV).Binding event of atrazine to anti-atrazine antibody,which immobilized on nanofiber-modified electrode via EDC and NHS chemistry,was transduced with EIS.Due to high conductivity,surface area,and low bandgap of MWCNT-ZnO nanofibers,we have achieved the sensitivity and limit of detection(LoD)of sensor as 21.61(KΩμg^(−1) mL^(−1))cm^(−2) and 5.368zM for a wide detection range of 10 zM–1µM.The proposed immunosensing platform has good stability,selectivity,repeatability,and reproducibility,and are less prone to interference.展开更多
基金supported by the Rural & Environment Science & Analytical Services (RESAS) Division of the Scottish Government through project JHI-B1-1the Biotechnology and Biological Sciences Research Council (BBSRC) through awards BB/ S015663/1+2 种基金BB/X009068/1Research Leaders 2025 fellowship funded by European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no. 754380the Research/Scientific Computing teams at The James Hutton Institute and NIAB for providing computational resources and technical support for the “UK’s Crop Diversity Bioinformatics HPC” (BBSRC grant BB/ S019669/1)。
文摘An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascertain at early infection stages owing to the unfavourable transcript representation compared to the host genes.In this study,we compare two sequencing techniques,RNAseq and enrichment sequencing(RenSeq and PenSeq)of cDNA,to investigate gene expression patterns in the doubled monoploid potato(DM)infected with the late blight pathogen Phytophthora infestans.Our results reveal distinct advantages of cDNA RenSeq and PenSeq over traditional RNAseq in terms of target gene representation and transcriptional quantification at early infection stages.Throughout the infection time course,cDNA enrichment sequencing enables transcriptomic analyses for more targeted host and pathogen genes.For highly expressed genes that were sampled in parallel by both cDNA enrichment and RNAseq,a high level of concordance in expression profiles is observed,indicative of at least semi-quantitative gene expression representation following enrichment.
文摘A 45-year-old female with known situs inversus totalis presented with left-sided abdominal discomfort. Chest X-ray, abdominal ultrasonography and CT scan confirmed the diagnosis of a gallstone, as well as, situs inversus;Laparoscopic cholecystectomy was safely performed with mirror image of standard 4 ports. Callots triangle dissection was done with epigastric working port by surgeon, but gallbladder fossa dissection was done by surgical assistant from midclavicular port as main working port. Laparoscopic surgeon should be careful for view of reversed relationships and also for existence of other anomalies.
基金support from Science Foundation Ireland Frontiers for the Future Award(19/FFP/6443)Science Foundation Ireland Strategic Partnership Program,Precision Oncology Ireland(18/SPP/3522)(L.S.Y.)+2 种基金Breast Cancer Now Fellowship Award with the generous support of Walk the Walk(2019AugSF1310)(D.V.)Science Foundation Ireland(20/FFP-P/8597)(D.V.)Breast Cancer Ireland program Grant(18239A01)(L.S.Y.).
文摘The expression of estrogen receptor(ER)and human epidermal growth factor receptor-2(HER2)in breast cancer can change in response to treatment and pivotally influence tumor behavior and clinical management[1].Receptor discordance has been observed at various distant metastatic site(bone,lung,liver,and brain),with a routine loss of ER and gains in HER2 reported[2].This receptor discordance can influence tumor responsiveness to both HER2 inhibitor and endocrine therapies.Although the mechanisms underlying receptor expression changes are not fully understood,we recently reported gains in ESR1 promoter hypermethylation as a potential driver of ER loss during disease progression[3].In this study,mechanisms underlying altered receptor expression and associated disease outcomes were examined following neoadjuvant trastuzumab treatment.
文摘In this study we have reported the design and development of a facile,sensitive,selective,and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers.Electrospun nanofibers were characterized using scanning electron microscope(SEM),transmission electron microscope(TEM),X-ray diffraction(XRD),X-ray photoelectron spectroscope(XPS),UV-Visible spectroscope(UV-VIS),and Fourier-transform infrared spectroscope(FTIR).Electrochemical properties of MWCNT-ZnO nanofiber-modified electrodes were assessed using electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV).Binding event of atrazine to anti-atrazine antibody,which immobilized on nanofiber-modified electrode via EDC and NHS chemistry,was transduced with EIS.Due to high conductivity,surface area,and low bandgap of MWCNT-ZnO nanofibers,we have achieved the sensitivity and limit of detection(LoD)of sensor as 21.61(KΩμg^(−1) mL^(−1))cm^(−2) and 5.368zM for a wide detection range of 10 zM–1µM.The proposed immunosensing platform has good stability,selectivity,repeatability,and reproducibility,and are less prone to interference.
