BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a disease of increasing global prevalence and an important risk factor for the development of insulin resistance,type 2 diabetes,non-alcoholic steatohepatitis and ...BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a disease of increasing global prevalence and an important risk factor for the development of insulin resistance,type 2 diabetes,non-alcoholic steatohepatitis and hepatocellular carcinoma,but the pathogenesis is not clear.The aim of this study was to explore the role of ILF3 in NAFLD.AIM To investigate the molecular processes through which ILF3 facilitates the advancement of NAFLD by inhibiting the expression of p-AMPK.This exploration seeks to provide new insights into the etiology of NAFLD and evaluate the potential of ILF3 as a diagnostic marker and potential treatment focus for future interventions.METHODS In vitro and in vivo experiments were conducted using HepG2 cells and NAFLD animal models.The effects of ILF3 knockdown on lipid synthesis and triglyceride(TG)secretion were examined by analyzing the expression levels of p-AMPK.Additionally,the roles of ILF3 and the AMPK signaling pathway were verified using techniques such as Western blotting,quantitative reverse transcription PCR,Oil Red O staining,and immunohistochemistry.RESULTS Investigations revealed an increase in ILF3 Levels within both HepG2 cells and animal models of NAFLD,concurrently with a decrease in p-AMPK expression.Knocking down ILF3 activated the AMPK pathway,reducing lipid production and TG secretion in hepatocytes,thereby mitigating the advancement of NAFLD.CONCLUSION ILF3 promotes the evolution of NAFLD by inhibiting the expression of p-AMPK.The knockdown of ILF3 activates the AMPK signaling pathway,alleviating the severity of NAFLD.These findings underscore the function of ILF3 in the pathogenesis of NAFLD and demonstrate its viability as a treatment focus and diagnostic indicator.展开更多
The high‐voltage isolated energy supply transformer(HIET)is the key component of the HVDC breaker,which is used for energy transmission and the potential isolation.There is a lack of research results that can meet en...The high‐voltage isolated energy supply transformer(HIET)is the key component of the HVDC breaker,which is used for energy transmission and the potential isolation.There is a lack of research results that can meet engineering applications at home and abroad.The design of a 500 kV HIET with dry‐type insulation is proposed,using 10 cascade connected 50 kV sub‐transformers(STs),which uses silicon rubber suitable for DC voltage as the main insulation material.High‐voltage(HV)winding of ST adopts double‐shielding structure of coil semi‐conductive shielding and inner semi‐conductive shielding to improve electric field distribution.The simulation analysis of an electric field at different insulation thicknesses and temperatures shows that the electric field decreases with an increase in thickness of insulation.When the thickness is greater than 25 mm,the electric field reduction effect is significantly weakened.The electric field is closely related to temperature,when at 20℃,the electric field between double‐shielding is relatively uniform.When at 70℃,the inner shielding electric field is much higher than coil shielding,and undergoes obvious reversal.In addition,the 500 kV HIET's electric field has been analysed.The 500kV HIET prototype has been manufactured and passed the type test,and successfully applied to the 500 kV Zhangbei DC grid project.展开更多
基金Supported by the Wuhan Science and Technology Bureau Project,No.2022020801020552(to Zhan T)Wuhan Health and Family Planning Commission Medical Research Project,No.WX20M01(to Tian X).
文摘BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is a disease of increasing global prevalence and an important risk factor for the development of insulin resistance,type 2 diabetes,non-alcoholic steatohepatitis and hepatocellular carcinoma,but the pathogenesis is not clear.The aim of this study was to explore the role of ILF3 in NAFLD.AIM To investigate the molecular processes through which ILF3 facilitates the advancement of NAFLD by inhibiting the expression of p-AMPK.This exploration seeks to provide new insights into the etiology of NAFLD and evaluate the potential of ILF3 as a diagnostic marker and potential treatment focus for future interventions.METHODS In vitro and in vivo experiments were conducted using HepG2 cells and NAFLD animal models.The effects of ILF3 knockdown on lipid synthesis and triglyceride(TG)secretion were examined by analyzing the expression levels of p-AMPK.Additionally,the roles of ILF3 and the AMPK signaling pathway were verified using techniques such as Western blotting,quantitative reverse transcription PCR,Oil Red O staining,and immunohistochemistry.RESULTS Investigations revealed an increase in ILF3 Levels within both HepG2 cells and animal models of NAFLD,concurrently with a decrease in p-AMPK expression.Knocking down ILF3 activated the AMPK pathway,reducing lipid production and TG secretion in hepatocytes,thereby mitigating the advancement of NAFLD.CONCLUSION ILF3 promotes the evolution of NAFLD by inhibiting the expression of p-AMPK.The knockdown of ILF3 activates the AMPK signaling pathway,alleviating the severity of NAFLD.These findings underscore the function of ILF3 in the pathogenesis of NAFLD and demonstrate its viability as a treatment focus and diagnostic indicator.
文摘The high‐voltage isolated energy supply transformer(HIET)is the key component of the HVDC breaker,which is used for energy transmission and the potential isolation.There is a lack of research results that can meet engineering applications at home and abroad.The design of a 500 kV HIET with dry‐type insulation is proposed,using 10 cascade connected 50 kV sub‐transformers(STs),which uses silicon rubber suitable for DC voltage as the main insulation material.High‐voltage(HV)winding of ST adopts double‐shielding structure of coil semi‐conductive shielding and inner semi‐conductive shielding to improve electric field distribution.The simulation analysis of an electric field at different insulation thicknesses and temperatures shows that the electric field decreases with an increase in thickness of insulation.When the thickness is greater than 25 mm,the electric field reduction effect is significantly weakened.The electric field is closely related to temperature,when at 20℃,the electric field between double‐shielding is relatively uniform.When at 70℃,the inner shielding electric field is much higher than coil shielding,and undergoes obvious reversal.In addition,the 500 kV HIET's electric field has been analysed.The 500kV HIET prototype has been manufactured and passed the type test,and successfully applied to the 500 kV Zhangbei DC grid project.
