BACKGROUND Intrauterine adhesion(IUA)can cause serious damage to women’s reproductive health,yet current treatment methods are difficult to achieve satisfactory results.In our previous studies,we demonstrated that me...BACKGROUND Intrauterine adhesion(IUA)can cause serious damage to women’s reproductive health,yet current treatment methods are difficult to achieve satisfactory results.In our previous studies,we demonstrated that menstrual-derived stromal stem cells(MenSCs),with high proliferative capacity and self-renewal ability,have a powerful therapeutic effect in patients with severe IUA.However,safety assessment of MenSCs transplantation is essential for its further application.AIM To evaluate the short-,medium-,and long-term biosafety of MenSCs via intrauterine transplantation in a rat model of IUA,with a focus on toxicity and tumorigenicity.METHODS MenSCs were injected into the sub-serosal layer of the uterus in an IUA rat model,for 3 d,3 mo,and 6 mo separately,to monitor the corresponding acute,sub-chronic,and chronic effects.Healthy rats of the same age served as negative controls.Toxicity effects were evaluated by body weight,organ weight,histopathology,hematology,and biochemistry tests.Tumorigenicity of MenSCs was investigated in Balb/c-nu mice in vivo and by colony formation assays in vitro.RESULTS Compared with the same week-old control group,all of the IUA rats receiving MenSC transplantation demonstrated no obvious changes in body weight,mainorgan weight,or blood cell composition during the acute,sub-chronic,and chronic observation periods.At the same time,serum biochemical tests showed no adverse effects on metabolism or liver and kidney function.After 4 wk of subcutaneous injection of Men SCs in Balb/c-nu nude mice,no tumor formation or cell metastasis was observed.Moreover,there was no tumor colony formation of Men SCs during soft agar culture in vitro.CONCLUSION There is no acute,sub-chronic,or chronic poisoning,infection,tumorigenesis,or endometriosis in rats with IUA after Men SC transplantation.The above results suggest that intrauterine transplantation of Men SCs is safe for endometrial treatment.展开更多
The hole transport layer(HTL)affects the device performance and stability of organic solar cells.In this work,a stable molybdenum oxide(MoO_(x))hole transport layer with low cost was prepared by adjusting the state of...The hole transport layer(HTL)affects the device performance and stability of organic solar cells.In this work,a stable molybdenum oxide(MoO_(x))hole transport layer with low cost was prepared by adjusting the state of the precursor solution with an alcoholic solution of molybdenum acetylacetonate through an oxidant.The MoO_(x) transport layer has good transmittance with a work function of 5.07 eV and higher surface energy.The PM6:Y6 devices using MoO_(x) HTL achieve a high efficiency of 16.8%.MoO_(x) HTL exhibits good applicability with excellent performance in both ternary and all-polymer systems.Air storage stability T80 of the all-polymer device using MoO_(x) HTL was over 600 h,much higher than 70 h of the PEDOT:PSS-based device,and its thermal stability at 85℃ and operational stability under light show better stability than that of the PEDOT:PSS hole transport layer.This work provides a facile and low-cost method to fabricate HTL for organic solar cells,which is beneficial to improve their efficiency and stability.展开更多
基金Supported by the National Key Research and Development Program,No.2018YFC1002105the Key Research and Development Program of Liaoning Province,No.2018020222the Major Special Construction Plan for Discipline Construction Project of China Medical University,No.3110118033。
文摘BACKGROUND Intrauterine adhesion(IUA)can cause serious damage to women’s reproductive health,yet current treatment methods are difficult to achieve satisfactory results.In our previous studies,we demonstrated that menstrual-derived stromal stem cells(MenSCs),with high proliferative capacity and self-renewal ability,have a powerful therapeutic effect in patients with severe IUA.However,safety assessment of MenSCs transplantation is essential for its further application.AIM To evaluate the short-,medium-,and long-term biosafety of MenSCs via intrauterine transplantation in a rat model of IUA,with a focus on toxicity and tumorigenicity.METHODS MenSCs were injected into the sub-serosal layer of the uterus in an IUA rat model,for 3 d,3 mo,and 6 mo separately,to monitor the corresponding acute,sub-chronic,and chronic effects.Healthy rats of the same age served as negative controls.Toxicity effects were evaluated by body weight,organ weight,histopathology,hematology,and biochemistry tests.Tumorigenicity of MenSCs was investigated in Balb/c-nu mice in vivo and by colony formation assays in vitro.RESULTS Compared with the same week-old control group,all of the IUA rats receiving MenSC transplantation demonstrated no obvious changes in body weight,mainorgan weight,or blood cell composition during the acute,sub-chronic,and chronic observation periods.At the same time,serum biochemical tests showed no adverse effects on metabolism or liver and kidney function.After 4 wk of subcutaneous injection of Men SCs in Balb/c-nu nude mice,no tumor formation or cell metastasis was observed.Moreover,there was no tumor colony formation of Men SCs during soft agar culture in vitro.CONCLUSION There is no acute,sub-chronic,or chronic poisoning,infection,tumorigenesis,or endometriosis in rats with IUA after Men SC transplantation.The above results suggest that intrauterine transplantation of Men SCs is safe for endometrial treatment.
基金the National Natural Science Foundation of China(No.21922505)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000).
文摘The hole transport layer(HTL)affects the device performance and stability of organic solar cells.In this work,a stable molybdenum oxide(MoO_(x))hole transport layer with low cost was prepared by adjusting the state of the precursor solution with an alcoholic solution of molybdenum acetylacetonate through an oxidant.The MoO_(x) transport layer has good transmittance with a work function of 5.07 eV and higher surface energy.The PM6:Y6 devices using MoO_(x) HTL achieve a high efficiency of 16.8%.MoO_(x) HTL exhibits good applicability with excellent performance in both ternary and all-polymer systems.Air storage stability T80 of the all-polymer device using MoO_(x) HTL was over 600 h,much higher than 70 h of the PEDOT:PSS-based device,and its thermal stability at 85℃ and operational stability under light show better stability than that of the PEDOT:PSS hole transport layer.This work provides a facile and low-cost method to fabricate HTL for organic solar cells,which is beneficial to improve their efficiency and stability.
