Chlorpyrifos is a pesticide widely used in agricultural production with a relatively long residual half-life in soil.Addressing the problem of residual chlorpyrifos is of universal concern.In this study,rice hull bioc...Chlorpyrifos is a pesticide widely used in agricultural production with a relatively long residual half-life in soil.Addressing the problem of residual chlorpyrifos is of universal concern.In this study,rice hull biochar was used as an immobilized carrier to prepare the immobilized strain H27 for the remediation of chlorpyrifos-contamination soil.Soil microorganisms after remediation were investigated by ecotoxicological methods.The immobilized strain H27 had the highest removal rate of chlorpyrifos when 10%bacterial solution was added to the liquid medium containing 0.075-0.109 mm diameter biochar cultured for 22 hr.This study on the removal of chlorpyrifos by immobilized strain H27 showed that the initial concentration of chlorpyrifos in solution was 25mg/L,and the removal rate reached 97.4%after 7 days of culture.In the soil,the removal rate of the immobilized bacteria group increased throughout the experiment,which was significantly higher than that of the free bacteria and biochar treatment groups.The Biolog-ECO test,T-RFLP and RT-RCR were used to study the effects of the soil microbial community and nitrogen cycling functional genes during chlorpyrifos degradation.It was found that ICP group had the highest diversity index among the four treatment groups.The microflora of segment containing 114 bp was the dominant bacterial community,and the dominant microflora of the immobilized bacteria group was more evenly distributed.The influence of each treatment group on ammonia-oxidizing bacteria(AOB)was greater than on ammonia-oxidizing archaea(AOA).This study offers a sound scientific basis for the practical application of immobilized bacteria to reduce residual soil pesticides.展开更多
Large commercial complexes are large in scale, complex in function, and located in densely populated areas that are prone to casualties due to unfavorable evacuation. To comprehend the safety evacuation of large comme...Large commercial complexes are large in scale, complex in function, and located in densely populated areas that are prone to casualties due to unfavorable evacuation. To comprehend the safety evacuation of large commercial complex buildings in China, investigate the safety evacuation problems encountered during the evacuation process and the evacuation optimization design strategy, the paper uses Pathfinder to build a simulation model based on literature research and study to simulate the evacuation of personnel in a large commercial complex in Dalian and explore its problems during the evacuation process. The results show that the type of personnel has an effect on the large commercial complex’s evacuation simulation results;the total number of evacuees is non-linearly correlated with the time change curve;some staircases take a long time to evacuate and have a low utilization rate. To improve evacuation efficiency, optimization suggestions for safety exits, evacuation stairs, and evacuation channels are made based on the results.展开更多
In-depth knowledge on the role of pyridine as a bottleneck restricting the successful application of anammox-based process treating refractory coking wastewater remains unknown.In this study,the effect of short-term p...In-depth knowledge on the role of pyridine as a bottleneck restricting the successful application of anammox-based process treating refractory coking wastewater remains unknown.In this study,the effect of short-term pyridine addition on a simultaneous anammox and denitrification(SAD)system fed with 25–150 mg/L pyridine was explored.The short-term operation showed that the highest total nitrogen(TN)removal efficiency was achieved at 25–50 mg/L of pyridine.As the pyridine addition increased,the contribution of the anammox pathway in nitrogen removal decreased from 99.3%to 79.1%,while the denitrification capability gradually improved.The specific anammox activity(SAA)at 150 mg/L pyridine decreased by 56.7%of the maximum SAA.The modified non-competitive inhibition model indicated that the 50%inhibitory concentration(IC_(50))of pyridine on anammox was 84.18 mg/L and the substrate inhibition constant(Ki)of pyridine for self-degradation was 135.19 mg/L according to the Haldane model.Moreover,high-throughput sequencing confirmed the abundance of Candidatus Kuenenia as the amount of anammox species decreased,while the amounts of denitrifiers and pyridine degraders significantly increased as the pyridine stress increased.Finally,the possible pathways of nitrogen bioconversion and pyridine biodegradation in the SAD system were elucidated through metagenomic analysis and gas chromatography/mass spectrometry results.The findings of this study enlarge the understanding of the removal mechanisms of complex nitrogenous pyridinecontaining wastewater treated by the SAD process.展开更多
Adhesion of P-type and type-1 fimbriated uropathogenic E.coli(UPEC)to uroepithelial cells initiates urinary tract infections(UTIs).This research aimed to evaluate the capacities of selected cranberry polyphenols and t...Adhesion of P-type and type-1 fimbriated uropathogenic E.coli(UPEC)to uroepithelial cells initiates urinary tract infections(UTIs).This research aimed to evaluate the capacities of selected cranberry polyphenols and their microbial metabolites to inhibit such adhesion in vitro using a modified fluorometric method.Data showed that the inhibition capacity of myricetin increased with concentration and plateaued at 70%.It had IC50 values of 13.2μM against P-type E.coli and 5.50μM against type-1 E.coli.Quercetin showed similar anti-adhesion capacities to myricetin.Procyanidin A2 and B2 had weaker anti-adhesion activities than myricetin and quercetin,with maximal inhibition capacities of 20%-30%against UPEC.Hippuric acid,a major metabolite of cranberry polyphenols in human urine,showed a maximal inhibition of 20%at 558μM against type-1 E.coli adhesion,whereas no anti-adhesion activity against P-type E.coli was detected.The fractions of cranberry fruit powder enriched with proanthocyanidin polymers showed the highest anti-adhesion activities compared to the fractions enriched with anthocyanins,flavonols,or proanthocyanidin oligomers.Overall,the anti-adhesion activities of cranberry polyphenols and metabolites depend on their structures and the types of fimbriae on E.coli.展开更多
Aims:In this study,we aimed to identify cuproptosis-associated ferroptosis genes in the atherosclerosis microarray of the Gene Expression Omnibus(GEO)database and to explore hub gene-mediated immune infiltration in at...Aims:In this study,we aimed to identify cuproptosis-associated ferroptosis genes in the atherosclerosis microarray of the Gene Expression Omnibus(GEO)database and to explore hub gene-mediated immune infiltration in atherosclerosis.Background:Immune infiltration plays a crucial role in atherosclerosis development.Ferroptosis is a mode of cell death caused by the iron-dependent accumulation of lipid peroxides.Cuproptosis is a recently discovered type of programmed cell death.No previous studies have examined the mechanism of cuproptosis-associated ferroptosis gene regulation in immune infiltration in atherosclerosis.Methods:We searched the qualified atherosclerosis gene microarray in the GEO database,integrated it with ferroptosis and cuproptosis genes,and calculated the correlation coefficients.We then obtained the cuproptosis-associated ferrop-tosis gene matrix and screened differentially expressed genes.Subsequently,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses and protein–protein interaction network analysis of differen-tially expressed genes.We also screened hub genes according to the Matthews correlation coefficient(MCC)algorithm.We conducted enrichment analysis of hub genes to explore their functions and predict related microRNAs(P<0.05).We also used the single-sample gene set enrichment analysis(ssGSEA)algorithm to analyze the relationships between hub genes and immune infiltration,and used immune-associated hub genes to construct a risk model.Finally,we used the drug prediction results and molecular docking technology to explore potential therapeutic drugs targeting the hub genes.Results:Seventy-eight cuproptosis-associated ferroptosis genes were found to be involved in the cellular response to oxidative and chemical stress,and to be enriched in multiple pathways,including ferroptosis,glutathione metabolism,and atherosclerosis.Ten hub genes were identified with the MCC algorithm;according to the ssGSEA algorithm,these genes were closely associated with immune infiltration,thus indicating that cuproptosis-associated ferroptosis genes may participate in atherosclerosis by mediating immune infiltration.The receiver operating characteristic curve indi-cated that the model had a good ability to predict atherosclerosis risk.The results of drug prediction(adjusted P<0.001)and molecular docking showed that glutathione may be a potential therapeutic drug that targets the hub genes.Conclusion:Cuproptosis-associated ferroptosis genes are associated with immune infiltration in atherosclerosis.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.42277039 and 42207026)the Natural Science Foundation of Shandong Province,China(No.ZR2022QD098)the Special Funds of Taishan Scholar of Shandong Province,China.
文摘Chlorpyrifos is a pesticide widely used in agricultural production with a relatively long residual half-life in soil.Addressing the problem of residual chlorpyrifos is of universal concern.In this study,rice hull biochar was used as an immobilized carrier to prepare the immobilized strain H27 for the remediation of chlorpyrifos-contamination soil.Soil microorganisms after remediation were investigated by ecotoxicological methods.The immobilized strain H27 had the highest removal rate of chlorpyrifos when 10%bacterial solution was added to the liquid medium containing 0.075-0.109 mm diameter biochar cultured for 22 hr.This study on the removal of chlorpyrifos by immobilized strain H27 showed that the initial concentration of chlorpyrifos in solution was 25mg/L,and the removal rate reached 97.4%after 7 days of culture.In the soil,the removal rate of the immobilized bacteria group increased throughout the experiment,which was significantly higher than that of the free bacteria and biochar treatment groups.The Biolog-ECO test,T-RFLP and RT-RCR were used to study the effects of the soil microbial community and nitrogen cycling functional genes during chlorpyrifos degradation.It was found that ICP group had the highest diversity index among the four treatment groups.The microflora of segment containing 114 bp was the dominant bacterial community,and the dominant microflora of the immobilized bacteria group was more evenly distributed.The influence of each treatment group on ammonia-oxidizing bacteria(AOB)was greater than on ammonia-oxidizing archaea(AOA).This study offers a sound scientific basis for the practical application of immobilized bacteria to reduce residual soil pesticides.
文摘Large commercial complexes are large in scale, complex in function, and located in densely populated areas that are prone to casualties due to unfavorable evacuation. To comprehend the safety evacuation of large commercial complex buildings in China, investigate the safety evacuation problems encountered during the evacuation process and the evacuation optimization design strategy, the paper uses Pathfinder to build a simulation model based on literature research and study to simulate the evacuation of personnel in a large commercial complex in Dalian and explore its problems during the evacuation process. The results show that the type of personnel has an effect on the large commercial complex’s evacuation simulation results;the total number of evacuees is non-linearly correlated with the time change curve;some staircases take a long time to evacuate and have a low utilization rate. To improve evacuation efficiency, optimization suggestions for safety exits, evacuation stairs, and evacuation channels are made based on the results.
基金supported by the National Natural Science Foundation of China(Grant No.21607111)the Key Research and Development Projects of Shanxi Province(No.201803D31052)the Opening Project of National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology and State Key Laboratory of Pollution Control,Resource Reuse Foundation,Tongji University(No.PCRRF18011).
文摘In-depth knowledge on the role of pyridine as a bottleneck restricting the successful application of anammox-based process treating refractory coking wastewater remains unknown.In this study,the effect of short-term pyridine addition on a simultaneous anammox and denitrification(SAD)system fed with 25–150 mg/L pyridine was explored.The short-term operation showed that the highest total nitrogen(TN)removal efficiency was achieved at 25–50 mg/L of pyridine.As the pyridine addition increased,the contribution of the anammox pathway in nitrogen removal decreased from 99.3%to 79.1%,while the denitrification capability gradually improved.The specific anammox activity(SAA)at 150 mg/L pyridine decreased by 56.7%of the maximum SAA.The modified non-competitive inhibition model indicated that the 50%inhibitory concentration(IC_(50))of pyridine on anammox was 84.18 mg/L and the substrate inhibition constant(Ki)of pyridine for self-degradation was 135.19 mg/L according to the Haldane model.Moreover,high-throughput sequencing confirmed the abundance of Candidatus Kuenenia as the amount of anammox species decreased,while the amounts of denitrifiers and pyridine degraders significantly increased as the pyridine stress increased.Finally,the possible pathways of nitrogen bioconversion and pyridine biodegradation in the SAD system were elucidated through metagenomic analysis and gas chromatography/mass spectrometry results.The findings of this study enlarge the understanding of the removal mechanisms of complex nitrogenous pyridinecontaining wastewater treated by the SAD process.
基金funded in part by University of Florida Research Foundation.TEM images were acquired by Nicole J.Machi and Rodolfo Alvarado in the Interdisciplinary Center for Biotechnology Research at University of Florida.
文摘Adhesion of P-type and type-1 fimbriated uropathogenic E.coli(UPEC)to uroepithelial cells initiates urinary tract infections(UTIs).This research aimed to evaluate the capacities of selected cranberry polyphenols and their microbial metabolites to inhibit such adhesion in vitro using a modified fluorometric method.Data showed that the inhibition capacity of myricetin increased with concentration and plateaued at 70%.It had IC50 values of 13.2μM against P-type E.coli and 5.50μM against type-1 E.coli.Quercetin showed similar anti-adhesion capacities to myricetin.Procyanidin A2 and B2 had weaker anti-adhesion activities than myricetin and quercetin,with maximal inhibition capacities of 20%-30%against UPEC.Hippuric acid,a major metabolite of cranberry polyphenols in human urine,showed a maximal inhibition of 20%at 558μM against type-1 E.coli adhesion,whereas no anti-adhesion activity against P-type E.coli was detected.The fractions of cranberry fruit powder enriched with proanthocyanidin polymers showed the highest anti-adhesion activities compared to the fractions enriched with anthocyanins,flavonols,or proanthocyanidin oligomers.Overall,the anti-adhesion activities of cranberry polyphenols and metabolites depend on their structures and the types of fimbriae on E.coli.
基金This study was funded by the Education Department of Hebei Province(project No.QN2016145)a student research project at Chengde Medical University(project No.2021101,2022086)+1 种基金the university-level scientific research project in CDMC(project No.202118)Fundamental Research Funds for Chengde Medical University(project No.KY202220).
文摘Aims:In this study,we aimed to identify cuproptosis-associated ferroptosis genes in the atherosclerosis microarray of the Gene Expression Omnibus(GEO)database and to explore hub gene-mediated immune infiltration in atherosclerosis.Background:Immune infiltration plays a crucial role in atherosclerosis development.Ferroptosis is a mode of cell death caused by the iron-dependent accumulation of lipid peroxides.Cuproptosis is a recently discovered type of programmed cell death.No previous studies have examined the mechanism of cuproptosis-associated ferroptosis gene regulation in immune infiltration in atherosclerosis.Methods:We searched the qualified atherosclerosis gene microarray in the GEO database,integrated it with ferroptosis and cuproptosis genes,and calculated the correlation coefficients.We then obtained the cuproptosis-associated ferrop-tosis gene matrix and screened differentially expressed genes.Subsequently,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses and protein–protein interaction network analysis of differen-tially expressed genes.We also screened hub genes according to the Matthews correlation coefficient(MCC)algorithm.We conducted enrichment analysis of hub genes to explore their functions and predict related microRNAs(P<0.05).We also used the single-sample gene set enrichment analysis(ssGSEA)algorithm to analyze the relationships between hub genes and immune infiltration,and used immune-associated hub genes to construct a risk model.Finally,we used the drug prediction results and molecular docking technology to explore potential therapeutic drugs targeting the hub genes.Results:Seventy-eight cuproptosis-associated ferroptosis genes were found to be involved in the cellular response to oxidative and chemical stress,and to be enriched in multiple pathways,including ferroptosis,glutathione metabolism,and atherosclerosis.Ten hub genes were identified with the MCC algorithm;according to the ssGSEA algorithm,these genes were closely associated with immune infiltration,thus indicating that cuproptosis-associated ferroptosis genes may participate in atherosclerosis by mediating immune infiltration.The receiver operating characteristic curve indi-cated that the model had a good ability to predict atherosclerosis risk.The results of drug prediction(adjusted P<0.001)and molecular docking showed that glutathione may be a potential therapeutic drug that targets the hub genes.Conclusion:Cuproptosis-associated ferroptosis genes are associated with immune infiltration in atherosclerosis.
