Epithelial-mesenchymal transition is a well-defined,reversible process in which epithelial cells lose their epithelial phenotype and acquire mesenchymal-like features.Epithelial-mesenchymal transition contributes sign...Epithelial-mesenchymal transition is a well-defined,reversible process in which epithelial cells lose their epithelial phenotype and acquire mesenchymal-like features.Epithelial-mesenchymal transition contributes significantly to the metastasis,invasion,and development of treatment resistance in cancer cells.There have been many studies on suppression of tumor epithelial-mesenchymal transition by Chinese medicine in recent years,mainly based on Chinese herbal drug monomers and compounds.In this review,we aim to describe the research progress on Chinese medicine in the t ransforming growth factor beta(T GF-β)sig naling pathway,we hope these will provide some guidance for further research on Chinese medicine targeting epithelial-mesenchymal transition.展开更多
Silicon(Si)particles were functionalized using carbon dots(CDs)to enhance the interaction between the Si particles and the binders.First,CDs rich in polar groups were synthesized using a simple hydrothermal method.The...Silicon(Si)particles were functionalized using carbon dots(CDs)to enhance the interaction between the Si particles and the binders.First,CDs rich in polar groups were synthesized using a simple hydrothermal method.Then,CDs were loaded on the Si surface by impregnation to obtain the functionalized Si particles(Si/CDs).The phases and microstructures of the Si/CDs were observed using Fourier-transform infrared reflection,X-ray diffraction,scanning electron microscopy,and high-resolution transmission electron microscopy.Si/CDs were used as the active material of the anode for electrochemical performance experiments.The electrochemical performance of the Si/CD electrode was assessed using cyclic voltammetry,electrochemical impedance spectroscopy,and constant current charge and discharge experiment.The electrodes prepared with Si/CDs showed good mechanical structure stability and electrochemical performance.After 150 cycles at 0.2 C,the capacity retention rate of the Si/CD electrode was 64.0%,which is twice as much as that of pure Si electrode under the same test conditions.展开更多
AIM： To introduce a bioimpedance gastric motility mea- surement method based on an electrical-mechanical composite concept and a preliminary clinical application. METHODS： A noninvasive gastric motility measure- men...AIM： To introduce a bioimpedance gastric motility mea- surement method based on an electrical-mechanical composite concept and a preliminary clinical application. METHODS： A noninvasive gastric motility measure- ment method combining electrogastrograrn （EGG） and impedance gastric motility （IGM） test was used. Prelim- inary clinical application studies of patients with func- tional dyspepsia （FD） and gastritis, as well as healthy controls, were carried out. Twenty-eight FD patients （mean age 40.9±9.7 years） and 40 healthy volun- teers （mean age 30.9±7.9 years） were involved. IGM spectrum was measured for both the healthy subjects and FD patients, and outcomes were compared in the FD patients before treatment and 1 wk and 3 wk after treatment. IGM parameters were obtained from 30 erosive gastritis patients （mean age 50.5±13.0 years） and 40 healthy adults, and IGM and EGG results were compared in the gastritis patients before treatment and 1 wk after treatment.RESULTS： There were significant differences in the IGM parameters between the FD patients and healthy subjects, and FD patients had a poorer gastric motility [percentage of normal frequency （PNF） 70.8±25.5 in healthy subjects and 28.3 =t= 16.9 in FD patients, P 〈 0.01]. After 1 wk administration of domperidone 10 mg, tid, the gastric motility of FD patients was not im- proved, although the EGG of the patients had returned to normal. After 3 wk of treatment, the IGM rhythm of the FD patients became normal. There was a significant difference in IGM parameters between the two groups （PNF 70.4：1：25.5 for healthy subjects and 36.1 4- 21.8 for gastritis patients, P 〈 0.05）. The EGG rhythm of the gastritis patients returned to normal （frequency insta- bility coefficient 2.22±0.43 before treatment and 1.77 ：t： 0.19 one wk after treatment, P 〈 0.05） after 1 wk of treatment with sodium rabeprazole tablets, 10 mg, qd, po, qm, while some IGM parameters showed a tenden- cy toward improvement but had not reached statistical significance. CONCLUSION： The electrical-mechanical composite measurement method showed an attractive clinical appli- cation prospect in gastric motility research and evaluation.展开更多
Generally speaking, being an efficient information hiding scheme, what we want to achieve is high embedding capacity of the cover image and high visual quality of the stego image, high visual quality is also called em...Generally speaking, being an efficient information hiding scheme, what we want to achieve is high embedding capacity of the cover image and high visual quality of the stego image, high visual quality is also called embedding efficiency. This paper mainly studies on the information hiding technology based on gray-scale digital images and especially considers the improvement of embedding capacity and embedding efficiency. For the purpose of that, two algorithms for information hiding were proposed, one is called high capacity of information hiding algorithm (HCIH for short), which achieves high embedding rate, and the other is called high quality of information hiding algorithm (HQIH for short), which realizes high embedding efficiency. The simulation experiments show that our proposed algorithms achieve better performance.展开更多
The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and ...The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.展开更多
LiMn_(6) hexatomic-rings act as functional units in Li-rich layered oxides(LLOs),which determine the capacity,voltage,and structural stability of LLOs.However,the symmetry of the LiMn_(6) hexatomic-ring is always brok...LiMn_(6) hexatomic-rings act as functional units in Li-rich layered oxides(LLOs),which determine the capacity,voltage,and structural stability of LLOs.However,the symmetry of the LiMn_(6) hexatomic-ring is always broken,especially in the grain surface of LLOs,which will greatly affect its electrochemical performance.Herein,the symmetry-breaking of LiMn_(6) hexatomic-ring in the grain surface of Li_(2)MnO_(3) was studied,and their effect on charge compensation mechanism and structure evolution behavior was thoroughly investigated.The results show that the electrochemical activity of the symmetry-broken LiMn_(6) hexatomic-ring is higher than that of the unbroken LiMn_(6),and the former is more favorable for spinelization on the grain surface.Furthermore,the exposure proportion of crystallographic planes with different symmetry-broken LiMn_(6) hexatomic-ring has also been discussed,which can be adjusted by changing the partial pressure of oxygen.The in-depth understanding of the symmetry-breaking of LiMn_(6) hexatomic-ring will provide more targeted strategies for designing high-performance LLOs cathodes for lithium-ion batteries.展开更多
Background:This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A(TrkA)receptor in mammalian cells by using genetic code expansion technology...Background:This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A(TrkA)receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway.The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated.Methods:Two photosensitive unnatural amino acids,p-azido-L-phenylalanine(AzF)and photo-caged tyrosine(ONB)were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis.Western blotting and laser confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell differentiation before and after photostimulation.Results:Our results supplemented the light-controlled results of the TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in regulating the MAPK/ERK pathway.Conclusion:This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.展开更多
Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinica...Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.展开更多
The majority of the literature on the transformation of cultural promotion space in old residential areas in the United States and abroad is written from the top-down perspective of God,such as architects,planners,dev...The majority of the literature on the transformation of cultural promotion space in old residential areas in the United States and abroad is written from the top-down perspective of God,such as architects,planners,developers,and even government officials,and only a few of them examine the designer’s work from the perspective of aborigines.To sample life and gain insight into human nature,find another means to be as near to the public as possible,listen to the voice of users,and conduct an in-depth examination of the freestyle works altered by the old residential districts through the“people’s architectural planner.”展开更多
Heterogeneous graphs contain multiple types of entities and relations,which are capable of modeling complex interactions.Embedding on heterogeneous graphs has become an essential tool for analyzing and understanding s...Heterogeneous graphs contain multiple types of entities and relations,which are capable of modeling complex interactions.Embedding on heterogeneous graphs has become an essential tool for analyzing and understanding such graphs.Although these meticulously designed methods make progress,they are limited by model design and computational resources,making it difficult to scale to large-scale heterogeneous graph data and hindering the application and promotion of these methods.In this paper,we propose Restage,a relation structure-aware hierarchical heterogeneous graph embedding framework.Under this framework,embedding only a smaller-scale graph with existing graph representation learning methods is sufficient to obtain node representations on the original heterogeneous graph.We consider two types of relation structures in heterogeneous graphs:interaction relations and affiliation relations.Firstly,we design a relation structure-aware coarsening method to successively coarsen the original graph to the top-level layer,resulting in a smaller-scale graph.Secondly,we allow any unsupervised representation learning methods to obtain node embeddings on the top-level graph.Finally,we design a relation structure-aware refinement method to successively refine the node embeddings from the top-level graph back to the original graph,obtaining node embeddings on the original graph.Experimental results on three public heterogeneous graph datasets demonstrate the enhanced scalability of representation learning methods by the proposed Restage.On another large-scale graph,the speed of existing representation learning methods is increased by up to eighteen times at most.展开更多
The rapid expansion of renewable energies asks for great progress of energy-storage technologies for sustainable energy supplies,which raises the compelling demand of high-performance rechargeable batteries.To satisfy...The rapid expansion of renewable energies asks for great progress of energy-storage technologies for sustainable energy supplies,which raises the compelling demand of high-performance rechargeable batteries.To satisfy the huge demand from the coming energy-storage market,the resource and cost-effectiveness of rechargeable batteries become more and more important.Manganese(Mn)as a key transition element with advantages including high abundance,low cost,and low toxicity derives various kinds(spinels,layered oxides,polyanions,Prussian blue analogs,etc.)of high-performance Mn-based electrode materials,especially cathodes,for rechargeable batteries ranging from Li-ion batteries,Na-ion batteries,aqueous batteries,to multivalent metal-ion batteries.It is anticipated that Mn-based materials with Mn as the major transition-metal element will constitute a flourishing family of Mn-based rechargeable batteries(Mn RBs)for large-scale and differentiated energy-storage applications.On the other hand,several critical issues including Jahn-Teller effect,Mn dissolution,and O release greatly hinder the pace of Mn RBs,which require extensive material optimizations and battery/system improvements.This review aims to provide an investigation about Mn-based materials and batteries for the coming energy-storage demands,with compelling issues and challenges that must be overcome.展开更多
The pursuit of highly active oxygen evolution reaction(OER)catalysts,especially those free of noble metals,is a focal point in fuel cell research.Utilizing extensive density functional theory calculations,this study d...The pursuit of highly active oxygen evolution reaction(OER)catalysts,especially those free of noble metals,is a focal point in fuel cell research.Utilizing extensive density functional theory calculations,this study designed and evaluated the activity and stability of singleatom catalysts(SACs)composed of 3d,4d and 5d transition metals supported on tungsten-based MXene for OER applications.Results highlighted the exceptional OER performance of Ni@W_(2)CO_(2),Rh@W_(2)CO_(2)and Pt@W_(2)CO_(2),displaying remarkably low overpotentials The catalytic activity of TM@W_(2)CO_(2)SACs exhibited a robust correlation with surface properties,particularly the d-band center index and surface work function.Moreover Ni@W_(2)CO_(2),Rh@W_(2)CO_(2)and Pt@W_(2)CO_(2)emerged as promising candidates for OER and oxygen reduction reaction(ORR)bifunctional catalysis,while Pt@W_(2)CO_(2)and Rh@W_(2)CO_(2)showed high potential for OER and hydrogen evolution reaction(HER)bifunctional catalysis The effectiveness of tungsten-based MXene as a substrate for non-noble-metal SACs marks a breakthrough in OER catalyst design,driving advancements towards sustainable energy solutions and addressing critical challenges in energy conversion and storage.展开更多
Dear Editor,Major depressive disorder(MDD)is one of the most common psychiatric illnesses that significantly increase the risk of suicide.1 Stress-triggered dysfunctions of microglia have been identified as a commonly...Dear Editor,Major depressive disorder(MDD)is one of the most common psychiatric illnesses that significantly increase the risk of suicide.1 Stress-triggered dysfunctions of microglia have been identified as a commonly occurred pathological feature of MDD.1–3 Microglial dysfunction contributes to the pathogenesis of MDD via immunoresponses/neuroinflammation-mediated neural damage and pathological synapse loss-mediated neural circuit disruption.1 Although the involvement of microglia in MDD has been widely investigated,the molecular mechanisms underlying microglial dysfunction remain largely unknown.Recently,we identified glutaminase 1(Gls1)as one key protein that participates in microglial dysfunction.4–6 Gls1 catalyzes the hydrolysis of glutamine to produce glutamate in the brain.4 Besides its well-known role in excitatory neurotoxicity,we found Gls1 up-regulation in microglia in animal models of Alzheimer’s disease and ischemic stroke.4,7 Gls1 activates microglia to overproduce cytokines and release inflammatory extracellular vesicles,therefore leading to neuroinflammation in animal models of Alzheimer’s disease and ischemic stroke.4–6 More importantly,Gls1 has been found to be up-regulated in the brains of MDD patients,and microglial Gls1 deficiency mitigated LPS-induced depression-like behaviors.8 However,LPS exposure is not an appropriate model to mimic MDD phenotypes,leaving the involvement of Gls1 in MDD an undetermined question.展开更多
For some time now,the research on gastric motility and function has fallen behind in the amount of research on gastric endocrine,exocrine secretion,and gastric morphology.In this paper,a noninvasive method to study ga...For some time now,the research on gastric motility and function has fallen behind in the amount of research on gastric endocrine,exocrine secretion,and gastric morphology.In this paper,a noninvasive method to study gastric motility was developed,taking bioimpedance measurements over the gastric area simultaneously with the electrogastrography(EGG).This is based on the concept of observing and analyzing simultaneously the intrinsic electrical gastric activity(basic electric rhythm) and the mechanical gastric activity.Additionally,preliminary clinical studies of healthy subjects and subjects with functional dyspepsia(FD) and gastritis were carried out.The impedance gastric motility(IGM) measurements of the healthy and FD subjects were compared,along with the studies of the FD subjects before treatment and after one week and three weeks of treatment.We also compared IGM measurements of healthy subjects and subjects with erosive gastritis,along with the studies of the subjects with erosive gastritis before treatment and after one week of treatment.Results show that FD subjects have poor gastric motility(P<0.01).After a week of treatment,the gastric motility of FD subjects was not yet improved although the EGG had returned to normal by this time.By three weeks of treatment,the regular IGM rhythm returned in FD subjects.There was a significant difference of IGM parameters between the gastritis and healthy subjects(P<0.05).The EGG rhythm of the gastritis subjects returned to normal at one week post-treatment,while IGM parameters showed a trend to improvement(P>0.05),These results suggest the possibility of clinic application of the proposed method.展开更多
基金supported by the Science and Technology Project of Hebei Education Department(QN2018060)Hebei Natural Science Foundation(H2022209027)+2 种基金Tangshan Science and Technology Project(21130230C)and Tangshan Talent Funding Project(A202203021)the Central Government Guides Local Funds For Science a nd Technology Development(Free Exploration o f Basic Research):the synergistic effect of nuclear targeted PTX/CUR nanodelivery system combined photothermal/photodynamic on breast cancer(236Z2704G)Natural Science Foundation of Hebei Province(H2022209068).
文摘Epithelial-mesenchymal transition is a well-defined,reversible process in which epithelial cells lose their epithelial phenotype and acquire mesenchymal-like features.Epithelial-mesenchymal transition contributes significantly to the metastasis,invasion,and development of treatment resistance in cancer cells.There have been many studies on suppression of tumor epithelial-mesenchymal transition by Chinese medicine in recent years,mainly based on Chinese herbal drug monomers and compounds.In this review,we aim to describe the research progress on Chinese medicine in the t ransforming growth factor beta(T GF-β)sig naling pathway,we hope these will provide some guidance for further research on Chinese medicine targeting epithelial-mesenchymal transition.
基金financially supported by the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang,China(No.2019R01006)the National Key R&D Program of China(Grant No.2018YFB0104300).
文摘Silicon(Si)particles were functionalized using carbon dots(CDs)to enhance the interaction between the Si particles and the binders.First,CDs rich in polar groups were synthesized using a simple hydrothermal method.Then,CDs were loaded on the Si surface by impregnation to obtain the functionalized Si particles(Si/CDs).The phases and microstructures of the Si/CDs were observed using Fourier-transform infrared reflection,X-ray diffraction,scanning electron microscopy,and high-resolution transmission electron microscopy.Si/CDs were used as the active material of the anode for electrochemical performance experiments.The electrochemical performance of the Si/CD electrode was assessed using cyclic voltammetry,electrochemical impedance spectroscopy,and constant current charge and discharge experiment.The electrodes prepared with Si/CDs showed good mechanical structure stability and electrochemical performance.After 150 cycles at 0.2 C,the capacity retention rate of the Si/CD electrode was 64.0%,which is twice as much as that of pure Si electrode under the same test conditions.
基金Supported by The National Natural Science Foundation of China, No. 60471041 and 60901045
文摘AIM： To introduce a bioimpedance gastric motility mea- surement method based on an electrical-mechanical composite concept and a preliminary clinical application. METHODS： A noninvasive gastric motility measure- ment method combining electrogastrograrn （EGG） and impedance gastric motility （IGM） test was used. Prelim- inary clinical application studies of patients with func- tional dyspepsia （FD） and gastritis, as well as healthy controls, were carried out. Twenty-eight FD patients （mean age 40.9±9.7 years） and 40 healthy volun- teers （mean age 30.9±7.9 years） were involved. IGM spectrum was measured for both the healthy subjects and FD patients, and outcomes were compared in the FD patients before treatment and 1 wk and 3 wk after treatment. IGM parameters were obtained from 30 erosive gastritis patients （mean age 50.5±13.0 years） and 40 healthy adults, and IGM and EGG results were compared in the gastritis patients before treatment and 1 wk after treatment.RESULTS： There were significant differences in the IGM parameters between the FD patients and healthy subjects, and FD patients had a poorer gastric motility [percentage of normal frequency （PNF） 70.8±25.5 in healthy subjects and 28.3 =t= 16.9 in FD patients, P 〈 0.01]. After 1 wk administration of domperidone 10 mg, tid, the gastric motility of FD patients was not im- proved, although the EGG of the patients had returned to normal. After 3 wk of treatment, the IGM rhythm of the FD patients became normal. There was a significant difference in IGM parameters between the two groups （PNF 70.4：1：25.5 for healthy subjects and 36.1 4- 21.8 for gastritis patients, P 〈 0.05）. The EGG rhythm of the gastritis patients returned to normal （frequency insta- bility coefficient 2.22±0.43 before treatment and 1.77 ：t： 0.19 one wk after treatment, P 〈 0.05） after 1 wk of treatment with sodium rabeprazole tablets, 10 mg, qd, po, qm, while some IGM parameters showed a tenden- cy toward improvement but had not reached statistical significance. CONCLUSION： The electrical-mechanical composite measurement method showed an attractive clinical appli- cation prospect in gastric motility research and evaluation.
文摘Generally speaking, being an efficient information hiding scheme, what we want to achieve is high embedding capacity of the cover image and high visual quality of the stego image, high visual quality is also called embedding efficiency. This paper mainly studies on the information hiding technology based on gray-scale digital images and especially considers the improvement of embedding capacity and embedding efficiency. For the purpose of that, two algorithms for information hiding were proposed, one is called high capacity of information hiding algorithm (HCIH for short), which achieves high embedding rate, and the other is called high quality of information hiding algorithm (HQIH for short), which realizes high embedding efficiency. The simulation experiments show that our proposed algorithms achieve better performance.
基金sponsored by the Guangdong Provincial Natural Science Foundation,No.S2012010009592the Science and Technology Talent Foundation of Guangdong Provincial Natural Science Foundation,No.30900725+2 种基金the Joint Research Program by Southern Medical University-Shunde Guizhou Hospital,No.09000608the Science Foshan Municipal Key Project in Medical Sciences,No.201008063and the Shunde Medical Research Program,No.2011050
文摘The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.
基金financially supported by the National Key R&D Program of China (2022YFB2404400)the National Natural Science Foundation of China (92263206,and 22002004)“The Youth Beijing Scholars program” (PXM2021_014204_000023)。
文摘LiMn_(6) hexatomic-rings act as functional units in Li-rich layered oxides(LLOs),which determine the capacity,voltage,and structural stability of LLOs.However,the symmetry of the LiMn_(6) hexatomic-ring is always broken,especially in the grain surface of LLOs,which will greatly affect its electrochemical performance.Herein,the symmetry-breaking of LiMn_(6) hexatomic-ring in the grain surface of Li_(2)MnO_(3) was studied,and their effect on charge compensation mechanism and structure evolution behavior was thoroughly investigated.The results show that the electrochemical activity of the symmetry-broken LiMn_(6) hexatomic-ring is higher than that of the unbroken LiMn_(6),and the former is more favorable for spinelization on the grain surface.Furthermore,the exposure proportion of crystallographic planes with different symmetry-broken LiMn_(6) hexatomic-ring has also been discussed,which can be adjusted by changing the partial pressure of oxygen.The in-depth understanding of the symmetry-breaking of LiMn_(6) hexatomic-ring will provide more targeted strategies for designing high-performance LLOs cathodes for lithium-ion batteries.
文摘Background:This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A(TrkA)receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway.The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated.Methods:Two photosensitive unnatural amino acids,p-azido-L-phenylalanine(AzF)and photo-caged tyrosine(ONB)were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis.Western blotting and laser confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell differentiation before and after photostimulation.Results:Our results supplemented the light-controlled results of the TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in regulating the MAPK/ERK pathway.Conclusion:This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.
基金Supported by grants from the Natural Science Foundation of Heilongjiang Province(No.H201335)Development Center for Medical Science and Technology,Ministry of Health,China(No.W2012FZ105)
文摘Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.
文摘The majority of the literature on the transformation of cultural promotion space in old residential areas in the United States and abroad is written from the top-down perspective of God,such as architects,planners,developers,and even government officials,and only a few of them examine the designer’s work from the perspective of aborigines.To sample life and gain insight into human nature,find another means to be as near to the public as possible,listen to the voice of users,and conduct an in-depth examination of the freestyle works altered by the old residential districts through the“people’s architectural planner.”
基金supported by the National Natural Science Foundation of China(Nos.1876001,61602003,and 61673020)the National High Technology Research and Development Program(No.2017YFB1401903)the Provincial Natural Science Foundation of Anhui Province(No.1708085QF156).
文摘Heterogeneous graphs contain multiple types of entities and relations,which are capable of modeling complex interactions.Embedding on heterogeneous graphs has become an essential tool for analyzing and understanding such graphs.Although these meticulously designed methods make progress,they are limited by model design and computational resources,making it difficult to scale to large-scale heterogeneous graph data and hindering the application and promotion of these methods.In this paper,we propose Restage,a relation structure-aware hierarchical heterogeneous graph embedding framework.Under this framework,embedding only a smaller-scale graph with existing graph representation learning methods is sufficient to obtain node representations on the original heterogeneous graph.We consider two types of relation structures in heterogeneous graphs:interaction relations and affiliation relations.Firstly,we design a relation structure-aware coarsening method to successively coarsen the original graph to the top-level layer,resulting in a smaller-scale graph.Secondly,we allow any unsupervised representation learning methods to obtain node embeddings on the top-level graph.Finally,we design a relation structure-aware refinement method to successively refine the node embeddings from the top-level graph back to the original graph,obtaining node embeddings on the original graph.Experimental results on three public heterogeneous graph datasets demonstrate the enhanced scalability of representation learning methods by the proposed Restage.On another large-scale graph,the speed of existing representation learning methods is increased by up to eighteen times at most.
基金financially supported by the National Key R&D Program of China(2022YFB2404400)the National Natural Science Foundation of China(92263206,21875007,21975006,21974007,and U19A2018)+1 种基金the Youth Beijing Scholars program(PXM2021_014204_000023)the Beijing Natural Science Foundation(2222001 and KZ202010005007)。
文摘The rapid expansion of renewable energies asks for great progress of energy-storage technologies for sustainable energy supplies,which raises the compelling demand of high-performance rechargeable batteries.To satisfy the huge demand from the coming energy-storage market,the resource and cost-effectiveness of rechargeable batteries become more and more important.Manganese(Mn)as a key transition element with advantages including high abundance,low cost,and low toxicity derives various kinds(spinels,layered oxides,polyanions,Prussian blue analogs,etc.)of high-performance Mn-based electrode materials,especially cathodes,for rechargeable batteries ranging from Li-ion batteries,Na-ion batteries,aqueous batteries,to multivalent metal-ion batteries.It is anticipated that Mn-based materials with Mn as the major transition-metal element will constitute a flourishing family of Mn-based rechargeable batteries(Mn RBs)for large-scale and differentiated energy-storage applications.On the other hand,several critical issues including Jahn-Teller effect,Mn dissolution,and O release greatly hinder the pace of Mn RBs,which require extensive material optimizations and battery/system improvements.This review aims to provide an investigation about Mn-based materials and batteries for the coming energy-storage demands,with compelling issues and challenges that must be overcome.
基金financially supported by the National Natural Science Foundation of China(Nos.22002004 and 92263206)the National Key R&D Program of China(No.2022YFB2404400)+1 种基金The Youth Beijing Scholars program(No.PXM2021_014204_000023)the Science and Technology Projects of China Minmetals Corporation(No.2021ZXA03)。
文摘The pursuit of highly active oxygen evolution reaction(OER)catalysts,especially those free of noble metals,is a focal point in fuel cell research.Utilizing extensive density functional theory calculations,this study designed and evaluated the activity and stability of singleatom catalysts(SACs)composed of 3d,4d and 5d transition metals supported on tungsten-based MXene for OER applications.Results highlighted the exceptional OER performance of Ni@W_(2)CO_(2),Rh@W_(2)CO_(2)and Pt@W_(2)CO_(2),displaying remarkably low overpotentials The catalytic activity of TM@W_(2)CO_(2)SACs exhibited a robust correlation with surface properties,particularly the d-band center index and surface work function.Moreover Ni@W_(2)CO_(2),Rh@W_(2)CO_(2)and Pt@W_(2)CO_(2)emerged as promising candidates for OER and oxygen reduction reaction(ORR)bifunctional catalysis,while Pt@W_(2)CO_(2)and Rh@W_(2)CO_(2)showed high potential for OER and hydrogen evolution reaction(HER)bifunctional catalysis The effectiveness of tungsten-based MXene as a substrate for non-noble-metal SACs marks a breakthrough in OER catalyst design,driving advancements towards sustainable energy solutions and addressing critical challenges in energy conversion and storage.
基金supported in part by research grants from the National Natural Science Foundation of China(Nos.91949204 and 81830037 to J.C.Z.,Nos.81971145 and 82271477 to X.X.)Independent Original Basic Research Program of Tongji University(No.22120220596 to X.X.).
文摘Dear Editor,Major depressive disorder(MDD)is one of the most common psychiatric illnesses that significantly increase the risk of suicide.1 Stress-triggered dysfunctions of microglia have been identified as a commonly occurred pathological feature of MDD.1–3 Microglial dysfunction contributes to the pathogenesis of MDD via immunoresponses/neuroinflammation-mediated neural damage and pathological synapse loss-mediated neural circuit disruption.1 Although the involvement of microglia in MDD has been widely investigated,the molecular mechanisms underlying microglial dysfunction remain largely unknown.Recently,we identified glutaminase 1(Gls1)as one key protein that participates in microglial dysfunction.4–6 Gls1 catalyzes the hydrolysis of glutamine to produce glutamate in the brain.4 Besides its well-known role in excitatory neurotoxicity,we found Gls1 up-regulation in microglia in animal models of Alzheimer’s disease and ischemic stroke.4,7 Gls1 activates microglia to overproduce cytokines and release inflammatory extracellular vesicles,therefore leading to neuroinflammation in animal models of Alzheimer’s disease and ischemic stroke.4–6 More importantly,Gls1 has been found to be up-regulated in the brains of MDD patients,and microglial Gls1 deficiency mitigated LPS-induced depression-like behaviors.8 However,LPS exposure is not an appropriate model to mimic MDD phenotypes,leaving the involvement of Gls1 in MDD an undetermined question.
基金Project (Nos. 60471041 and 60901045) supported by the National Natural Science Foundation of China
文摘For some time now,the research on gastric motility and function has fallen behind in the amount of research on gastric endocrine,exocrine secretion,and gastric morphology.In this paper,a noninvasive method to study gastric motility was developed,taking bioimpedance measurements over the gastric area simultaneously with the electrogastrography(EGG).This is based on the concept of observing and analyzing simultaneously the intrinsic electrical gastric activity(basic electric rhythm) and the mechanical gastric activity.Additionally,preliminary clinical studies of healthy subjects and subjects with functional dyspepsia(FD) and gastritis were carried out.The impedance gastric motility(IGM) measurements of the healthy and FD subjects were compared,along with the studies of the FD subjects before treatment and after one week and three weeks of treatment.We also compared IGM measurements of healthy subjects and subjects with erosive gastritis,along with the studies of the subjects with erosive gastritis before treatment and after one week of treatment.Results show that FD subjects have poor gastric motility(P<0.01).After a week of treatment,the gastric motility of FD subjects was not yet improved although the EGG had returned to normal by this time.By three weeks of treatment,the regular IGM rhythm returned in FD subjects.There was a significant difference of IGM parameters between the gastritis and healthy subjects(P<0.05).The EGG rhythm of the gastritis subjects returned to normal at one week post-treatment,while IGM parameters showed a trend to improvement(P>0.05),These results suggest the possibility of clinic application of the proposed method.
