Nanotechnology is a key advanced technology enabling contribution,development,and sustainable impact on food,medicine,and agriculture sectors.Nanomaterials have potential to lead qualitative and quantitative productio...Nanotechnology is a key advanced technology enabling contribution,development,and sustainable impact on food,medicine,and agriculture sectors.Nanomaterials have potential to lead qualitative and quantitative production of healthier,safer,and high-quality functional foods which are perishable or semi-perishable in nature.Nanotechnologies are superior than conventional food processing technologies with increased shelf life of food products,preventing contamination,and production of enhanced food quality.This comprehensive review on nanotechnologies for functional food development describes the current trends and future perspectives of advanced nanomaterials in food sector considering processing,packaging,security,and storage.Applications of nanotechnologies enhance the food bioavailability,taste,texture,and consistency,achieved through modification of particle size,possible cluster formation,and surface charge of food nanomaterials.In addition,the nanodelivery-mediated nutraceuticals,synergistic action of nanomaterials in food protection,and the application of nanosensors in smart food packaging for monitoring the quality of the stored foods and the common methods employed for assessing the impact of nanomaterials in biological systems are also discussed.展开更多
Agro seeds are vulnerable to environmental stressors,adversely affecting seed vigor,crop growth,and crop productivity.Different agrochemical-based seed treatments enhance seed germination,but they can also cause damag...Agro seeds are vulnerable to environmental stressors,adversely affecting seed vigor,crop growth,and crop productivity.Different agrochemical-based seed treatments enhance seed germination,but they can also cause damage to the environment;therefore,sustainable technologies such as nano-based agrochemicals are urgently needed.Nanoagrochemicals can reduce the dose-dependent toxicity of seed treatment,thereby improving seed viability and ensuring the controlled release of nanoagrochemical active ingredients However,the applications of nanoagrochemicals to plants in the field raise concerns about nanomaterial safety,exposure levels,and toxicological implications to the environment and human health.In the present comprehensive review,the development,scope,challenges,and risk assessments of nanoagrochemicals on seed treatment are discussed.Moreover,the implementation obstacles for nanoagrochemicals use in seed treatments,their commercialization potential,and the need for policy regulations to assess possible risks are also discussed.Based on our knowledge,this is the first time that we have presented legendary literature to readers in order to help them gain a deeper understanding of upcoming nanotechnologies that may enable the development of future generation seed treatment agrochemical formulations,their scope,and potential risks associated with seed treatment.展开更多
Fibrinolytic enzymes are considered as a significant therapeutic agent for thrombosis.Keeping the quest for identification of effective fibrinolytic enzyme(s)from microbial source,a comparative study was done on six B...Fibrinolytic enzymes are considered as a significant therapeutic agent for thrombosis.Keeping the quest for identification of effective fibrinolytic enzyme(s)from microbial source,a comparative study was done on six Bacillus spp.isolated from fruit and vegetable waste.The fibrinolytic activities of the isolates were confirmed by a fibrin plate assay.Six potential isolates were identified by 16s rRNA sequencing as Bacillus altitudinus HEM05,Bacillus halotolerance ARKH1,Bacillus safensis CO05,Bacillus subtilis subsp.inaquorum BE1,Bacillus tequilensis SAS23 and Bacillus siamensis BHO27.Extracellular fibrinolytic enzymes were purified to homogeneity from all six isolates by acetone precipitation,anion exchange chromatography and gel filtration chromatography.Purified fibrinolytic enzymes showed fibrinolytic specific activity ranging from 60.92 U mg^(-1) to 220.19 U mg^(-1).Molecular weight(SDS PAGE)of purified enzymes ranged from 30,200 kDa to 69,500 kDa.Enzymes were effective over wide range of pH from 5 to 10,with optimum pH between 7 and 10.The enzymes were active between 37℃ to 60℃.Effect of inhibitory activity of metal ions,protease inhibitors and amidolytic activity on purified enzymes indicated fibrinolytic enzymes from Bacillus altitudinus HEM05,Bacillus halotolerance ARKH1,Bacillus safensis CO05 as plasmin serine protease,from Bacillus subtilis subsp.inaquorum BE1,Bacillus tequilensis SAS23 as subtilin chymotrypsin like metalloprotease and from Bacillus siamensis BHO27 as subtilin chymotrypsin.In vitro assays for the purified enzyme preparations from all six isolates confirmed enzymes could activate plasminogen and significantly degraded the fibrin net of blood clot.展开更多
基金funded by National Key R&D Program of China (2018YFC1706200)National Natural Science Fund of China (31571735,81522049)+4 种基金Shanghai Science and Technology Committee Project(17JC1404300)Zhejiang Provincial Ten Thousands Program for Leading Talents of Science and Technology Innovation (2018R52050)Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents,TCM Foundation for Distinguished Young Talents of Zhejiang Province (2020ZQ014)Project of Zhejiang Chinese Medical University (2019ZR15)Opening project of Zhejiang provincial preponderant and characteristic subject of Key University (Traditional Chinese Pharmacology),Zhejiang Chinese Medical University (ZYAOX2018004,ZYAOX2018019)
文摘Nanotechnology is a key advanced technology enabling contribution,development,and sustainable impact on food,medicine,and agriculture sectors.Nanomaterials have potential to lead qualitative and quantitative production of healthier,safer,and high-quality functional foods which are perishable or semi-perishable in nature.Nanotechnologies are superior than conventional food processing technologies with increased shelf life of food products,preventing contamination,and production of enhanced food quality.This comprehensive review on nanotechnologies for functional food development describes the current trends and future perspectives of advanced nanomaterials in food sector considering processing,packaging,security,and storage.Applications of nanotechnologies enhance the food bioavailability,taste,texture,and consistency,achieved through modification of particle size,possible cluster formation,and surface charge of food nanomaterials.In addition,the nanodelivery-mediated nutraceuticals,synergistic action of nanomaterials in food protection,and the application of nanosensors in smart food packaging for monitoring the quality of the stored foods and the common methods employed for assessing the impact of nanomaterials in biological systems are also discussed.
文摘Agro seeds are vulnerable to environmental stressors,adversely affecting seed vigor,crop growth,and crop productivity.Different agrochemical-based seed treatments enhance seed germination,but they can also cause damage to the environment;therefore,sustainable technologies such as nano-based agrochemicals are urgently needed.Nanoagrochemicals can reduce the dose-dependent toxicity of seed treatment,thereby improving seed viability and ensuring the controlled release of nanoagrochemical active ingredients However,the applications of nanoagrochemicals to plants in the field raise concerns about nanomaterial safety,exposure levels,and toxicological implications to the environment and human health.In the present comprehensive review,the development,scope,challenges,and risk assessments of nanoagrochemicals on seed treatment are discussed.Moreover,the implementation obstacles for nanoagrochemicals use in seed treatments,their commercialization potential,and the need for policy regulations to assess possible risks are also discussed.Based on our knowledge,this is the first time that we have presented legendary literature to readers in order to help them gain a deeper understanding of upcoming nanotechnologies that may enable the development of future generation seed treatment agrochemical formulations,their scope,and potential risks associated with seed treatment.
文摘Fibrinolytic enzymes are considered as a significant therapeutic agent for thrombosis.Keeping the quest for identification of effective fibrinolytic enzyme(s)from microbial source,a comparative study was done on six Bacillus spp.isolated from fruit and vegetable waste.The fibrinolytic activities of the isolates were confirmed by a fibrin plate assay.Six potential isolates were identified by 16s rRNA sequencing as Bacillus altitudinus HEM05,Bacillus halotolerance ARKH1,Bacillus safensis CO05,Bacillus subtilis subsp.inaquorum BE1,Bacillus tequilensis SAS23 and Bacillus siamensis BHO27.Extracellular fibrinolytic enzymes were purified to homogeneity from all six isolates by acetone precipitation,anion exchange chromatography and gel filtration chromatography.Purified fibrinolytic enzymes showed fibrinolytic specific activity ranging from 60.92 U mg^(-1) to 220.19 U mg^(-1).Molecular weight(SDS PAGE)of purified enzymes ranged from 30,200 kDa to 69,500 kDa.Enzymes were effective over wide range of pH from 5 to 10,with optimum pH between 7 and 10.The enzymes were active between 37℃ to 60℃.Effect of inhibitory activity of metal ions,protease inhibitors and amidolytic activity on purified enzymes indicated fibrinolytic enzymes from Bacillus altitudinus HEM05,Bacillus halotolerance ARKH1,Bacillus safensis CO05 as plasmin serine protease,from Bacillus subtilis subsp.inaquorum BE1,Bacillus tequilensis SAS23 as subtilin chymotrypsin like metalloprotease and from Bacillus siamensis BHO27 as subtilin chymotrypsin.In vitro assays for the purified enzyme preparations from all six isolates confirmed enzymes could activate plasminogen and significantly degraded the fibrin net of blood clot.
