The Health Action Process Approach(HAPA),developed by German psychologist Schwarzer in the 1980s,provides a comprehensive framework for understanding and influencing health behavior change.By dividing behavior change ...The Health Action Process Approach(HAPA),developed by German psychologist Schwarzer in the 1980s,provides a comprehensive framework for understanding and influencing health behavior change.By dividing behavior change into three dynamic stages-pre-intention,intention,and action-HAPA highlights the critical role of self-efficacy as a driving force in adopting healthier behaviors.This stage-based approach addresses the limitations of continuous health behavior models by emphasizing individual differentiation and recognizing that behavior change is not linear but progresses through distinct phases.HAPA theory has demonstrated significant potential in clinical applications,particularly in orthopedic postoperative patients.Its implementation facilitates the development of positive behavioral intentions,enhances self-efficacy,and supports sustained health action,ultimately improving patients’health outcomes and quality of life.Tailored interventions based on HAPA stages ensure that patients receive appropriate guidance and support throughout their recovery journey.Despite its successes,limitations remain.Future research should focus on expanding the application of HAPA to various orthopedic conditions and developing more targeted behavioral plans and health education programs to optimize patient rehabilitation.Additionally,further exploration is needed to sustain HAPA’s effectiveness during long-term recovery.Strengthening interdisciplinary collaboration and integrating HAPA with other theoretical models could create a more comprehensive health behavior education system,providing robust support for orthopedic patients and advancing their overall recovery and well-being.展开更多
In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, a...In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar Purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar Purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in Purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.展开更多
Connected autonomous vehicles(CAVs)are a promising paradigm for implementing intelligent transportation systems.However,in CAVs scenarios,the sensing blind areas cause serious safety hazards.Existing vehicle-to-vehicl...Connected autonomous vehicles(CAVs)are a promising paradigm for implementing intelligent transportation systems.However,in CAVs scenarios,the sensing blind areas cause serious safety hazards.Existing vehicle-to-vehicle(V2V)technology is difficult to break through the sensing blind area and ensure reliable sensing information.To overcome these problems,considering infrastructures as a means to extend the sensing range is feasible based on the integrated sensing and communication(ISAC)technology.The mmWave base station(mmBS)transmits multiple beams consisting of communication beams and sensing beams.The sensing beams are responsible for sensing objects within the CAVs blind area,while the communication beams are responsible for transmitting the sensed information to the CAVs.To reduce the impact of inter-beam interference,a joint multiple beamwidth and power allocation(JMBPA)algorithm is proposed.By maximizing the communication transmission rate under the sensing constraints.The proposed non-convex optimization problem is transformed into a standard difference of two convex functions(D.C.)problem.Finally,the superiority of the lutions.The average transmission rate of communication beams remains over 3.4 Gbps,showcasing a significant improvement compared to other algorithms.Moreover,the satisfaction of sensing services remains steady.展开更多
The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of b...The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93)broodfish had offspring.The estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.展开更多
As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre...As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decreasing,posing a major threat to crop production.To address these problems,we need to improve the endurance of major crops.展开更多
Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes(ARGs).However,knowledge about the diversity and abundance of class 1 integrons and gene cas...Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes(ARGs).However,knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited.In this study,we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods.Further,we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes.A total of 42 isolates were inti-positive among the 208 strains isolated from drinking water,which tended to confer multi-drug resistance compared with intll-negative isolates.The absolute abundance of the intl1 average i.15×10^(9) copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chiorine disinfection.Furthermore,nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water,mainly conferring resistance to aminoglycosides and trimethoprim.The treatment processes,especially liquid chlorine disinfection,reduced most of the ARGs carried by gene cassettes,though some of the ARG subtypes persisted along the treatment and distribution ike aac(6)-Il,aadA,and dfrB2.The antibiotic resistance gene cassette array laac(6')-Ⅱ arr was most frequently detected,especially in the chlorinated water.This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer,indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.展开更多
Currently three major problems seriously limit the practical application of can-cer photodynamic therapy(PDT):(i)the hypoxic tumor microenvironment(TME);(ii)low generation efficiency of toxic reactive oxygen species(R...Currently three major problems seriously limit the practical application of can-cer photodynamic therapy(PDT):(i)the hypoxic tumor microenvironment(TME);(ii)low generation efficiency of toxic reactive oxygen species(ROS)in aggre-gates and(iii)shallow tissue penetration depth of excitation light.Very limited approaches are available for addressing all the above three problems with a single design.Herein,a rational“three birds with one stone”molecular and nanoengi-neering strategy is demonstrated:a photodynamic nanoplatform U-Ir@PAA-ABS based on the covalent combination of lanthanide-doped upconversion nanoparti-cles(UCNPs)and an AIE-active dinuclear Ir(III)complex provides a low oxygen concentration-dependent type-I photochemical process upon 980 nm irradiation by Föster resonance energy transfer(FRET).U-Ir@PAA-ABS targets mitochondria and has excellent phototoxicity even in severe hypoxia environments upon 980 nm irradiation,inducing a dual-mode cell death mechanism by apoptosis and ferropto-sis.Taken together,the in vitro and in vivo results demonstrate a successful strategy for improving the efficacy of PDT against hypoxic tumors.展开更多
Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhan...Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.展开更多
Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in m...Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.展开更多
Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing i...Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance.Establishing the base-line susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies.Here we evaluated the baseline susceptibil-ity to broflanilide for 3 cosmopolitan lepidopteran pest species,Helicoverpa armigera,Plutella xylostella,and Spodoptera frugiperda.Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China,with median lethal concentrations(LCso)ranging between 0.209 and 0.684,0.076 and 0.336,and 0.075 and 0.219 mg/L for H.armigera,P xylostella,and S.frugiperda,respectively.Among-population variability in susceptibility to broflanilide was moderate for H.armigera(3.3-fold),P xylostella(4.4-fold),and S.frugiperda(2.9-fold).The recommended diagnostic concentrations for H.armigera,P xylostella,and S.frugiperda were 8,4,and 2 mg/L,re-spectively.Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P xylostella and 1 spinosyns-resistant strain of S.frugiperda.Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.展开更多
We develop the effective modification strategy based on molecular engineering of s-triazine and its derivatives to improve the photoelectric performance of all-inorganic perovskites(AIP)for the first time.The surface ...We develop the effective modification strategy based on molecular engineering of s-triazine and its derivatives to improve the photoelectric performance of all-inorganic perovskites(AIP)for the first time.The surface modification strategy with cyanuric acid successfully increases the PLQY of AIP from 40.55%to 88.15%,and significantly enhances the current of the AIP film under 3 V by almost 20-fold(from4.44 m A to 81.20 m A).This work has proven the effectiveness of improving the photoelectric performances of AIP via s-triazine and its derivatives and also suggested the potential risks of reducing the photoelectric performance of AIP due to inappropriate substituents in conjugated organic ligands.展开更多
A novel mechanism of the arylation of arenes via norbornene(NBE)relay palladation through meta-to para-selectivity was revealed via density functional theory(DFT)calculations.Our calculated results revealed that the r...A novel mechanism of the arylation of arenes via norbornene(NBE)relay palladation through meta-to para-selectivity was revealed via density functional theory(DFT)calculations.Our calculated results revealed that the reaction was initiated by a[mono-N-protected amino acid ligand(MPAA)–Pd]complex to activate at first the meta-C–H guided by the directing group(DG),and para-arylation was subsequently achieved by NBE relay palladation from meta-to para-position.Significantly,the palladium/norbornene(Pd/NBE)cooperative catalysis was catalyzed by a Pd–Ag bimetallic complex,which accounted for the experimental fact that no yield detected without Ag.The reaction pathway through para-to meta-selectivity was also investigated,while this pathway was kinetically unfavorable.The results revealed that the initial DG guided C–H site activation was the rate-determining step and played an important role in determining site-selectivity.The primary meta-activation was favorable in energy due to the less ring strain in the cyclic nitrile-coordinated C–H transition states in the meta position.Moreover,the perfect cooperation of a remote directing template and a transient mediator NBE through the alternating association with the Pd center achieved the relay through meta-to para-position.The present results provide a reasonable insight into the para-C–H arylation by the Pd/MPAA/NBE cooperative catalysis in conjunction with a precise DG and Ag(I)additive.展开更多
基金Youth Project of Inner Mongolia Medical University in 2022(YKD2022QN020)。
文摘The Health Action Process Approach(HAPA),developed by German psychologist Schwarzer in the 1980s,provides a comprehensive framework for understanding and influencing health behavior change.By dividing behavior change into three dynamic stages-pre-intention,intention,and action-HAPA highlights the critical role of self-efficacy as a driving force in adopting healthier behaviors.This stage-based approach addresses the limitations of continuous health behavior models by emphasizing individual differentiation and recognizing that behavior change is not linear but progresses through distinct phases.HAPA theory has demonstrated significant potential in clinical applications,particularly in orthopedic postoperative patients.Its implementation facilitates the development of positive behavioral intentions,enhances self-efficacy,and supports sustained health action,ultimately improving patients’health outcomes and quality of life.Tailored interventions based on HAPA stages ensure that patients receive appropriate guidance and support throughout their recovery journey.Despite its successes,limitations remain.Future research should focus on expanding the application of HAPA to various orthopedic conditions and developing more targeted behavioral plans and health education programs to optimize patient rehabilitation.Additionally,further exploration is needed to sustain HAPA’s effectiveness during long-term recovery.Strengthening interdisciplinary collaboration and integrating HAPA with other theoretical models could create a more comprehensive health behavior education system,providing robust support for orthopedic patients and advancing their overall recovery and well-being.
基金supported by the National Natural Science Foundation of China, No. 30270502
文摘In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar Purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar Purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in Purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.
基金China Tele-com Research Institute Project(Grants No.HQBYG2200147GGN00)National Key R&D Program of China(2020YFB1807600)National Natural Science Foundation of China(NSFC)(Grant No.62022020).
文摘Connected autonomous vehicles(CAVs)are a promising paradigm for implementing intelligent transportation systems.However,in CAVs scenarios,the sensing blind areas cause serious safety hazards.Existing vehicle-to-vehicle(V2V)technology is difficult to break through the sensing blind area and ensure reliable sensing information.To overcome these problems,considering infrastructures as a means to extend the sensing range is feasible based on the integrated sensing and communication(ISAC)technology.The mmWave base station(mmBS)transmits multiple beams consisting of communication beams and sensing beams.The sensing beams are responsible for sensing objects within the CAVs blind area,while the communication beams are responsible for transmitting the sensed information to the CAVs.To reduce the impact of inter-beam interference,a joint multiple beamwidth and power allocation(JMBPA)algorithm is proposed.By maximizing the communication transmission rate under the sensing constraints.The proposed non-convex optimization problem is transformed into a standard difference of two convex functions(D.C.)problem.Finally,the superiority of the lutions.The average transmission rate of communication beams remains over 3.4 Gbps,showcasing a significant improvement compared to other algorithms.Moreover,the satisfaction of sensing services remains steady.
基金The Science and Technology Project of Guangdong Province under contract No.2019B121201001the National Key R&D Program of China under contract No.2019YFD0901301+3 种基金the Fund of China-Vietnam Joint Survey on Fish Stocks in the Common Fishing Zone of the Beibu Gulfthe Central Public-interest Scientific Institution Basal Research Fund,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science under contract No.2021SD14the China-ASEAN Maritime Cooperation Fund(China-Vietnam Fishery Stock Enhancement and Conservation in Beibu Gulf)the Hainan Provincial Natural Science Foundation of China under contract No.320QN358.
文摘The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93)broodfish had offspring.The estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.
基金supported by the National Natural Science Foundation of China(32271541)the National Key Research and Development Program of China(2021YFD1200701)+1 种基金Key Project of Maize Germplasm Improvement(2022010202,B21HJ0509)supported by Pinduoduo-China Agricultural University Research Fund(PC2023A01004)。
文摘As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decreasing,posing a major threat to crop production.To address these problems,we need to improve the endurance of major crops.
基金financially supported by the National Natural Science Foundation of China(Nos.52025102,52192682,and 52200057)the Jiangsu Funding Program for Excellent Postdoctoral Talent(China)(No.2022ZB35).
文摘Class 1 integrons are vital mobile genetic elements involved in the environmental transmission of antibiotic resistance genes(ARGs).However,knowledge about the diversity and abundance of class 1 integrons and gene cassettes during drinking water treatment and distribution is still limited.In this study,we aimed to uncover the prevalence of class 1 integrons in the drinking water treatment and distribution systems with the combination of culture-dependent and culture-independent methods.Further,we applied the nanopore sequencing method to characterize the diversity and arrangement of ARGs carried by class 1 integron-associated gene cassettes.A total of 42 isolates were inti-positive among the 208 strains isolated from drinking water,which tended to confer multi-drug resistance compared with intll-negative isolates.The absolute abundance of the intl1 average i.15×10^(9) copies/L in the source water and underwent the most significant reduction of over 99.9% after liquid chiorine disinfection.Furthermore,nanopore sequencing revealed that the class 1 integron-associated gene cassettes carried 51 subtypes of ARGs in drinking water,mainly conferring resistance to aminoglycosides and trimethoprim.The treatment processes,especially liquid chlorine disinfection,reduced most of the ARGs carried by gene cassettes,though some of the ARG subtypes persisted along the treatment and distribution ike aac(6)-Il,aadA,and dfrB2.The antibiotic resistance gene cassette array laac(6')-Ⅱ arr was most frequently detected,especially in the chlorinated water.This study underlined that drinking water was potential reservoir for integron-mediated ARGs transfer,indicating that the health risks of resistance gene cassettes in class 1 integrons deserved urgent attention.
基金NSFC,Grant/Award Numbers:52073045,51773195Key Scientific and Technological Project of Jilin Province,Grant/Award Number:20190701010GH+2 种基金Development and Reform Commission of Jilin Province,Grant/Award Number:2020C035-5Changchun Science and Technology Bureau,Grant/Award Number:21ZGY19EPSRC,Grant/Award Number:EP/L02621X/1。
文摘Currently three major problems seriously limit the practical application of can-cer photodynamic therapy(PDT):(i)the hypoxic tumor microenvironment(TME);(ii)low generation efficiency of toxic reactive oxygen species(ROS)in aggre-gates and(iii)shallow tissue penetration depth of excitation light.Very limited approaches are available for addressing all the above three problems with a single design.Herein,a rational“three birds with one stone”molecular and nanoengi-neering strategy is demonstrated:a photodynamic nanoplatform U-Ir@PAA-ABS based on the covalent combination of lanthanide-doped upconversion nanoparti-cles(UCNPs)and an AIE-active dinuclear Ir(III)complex provides a low oxygen concentration-dependent type-I photochemical process upon 980 nm irradiation by Föster resonance energy transfer(FRET).U-Ir@PAA-ABS targets mitochondria and has excellent phototoxicity even in severe hypoxia environments upon 980 nm irradiation,inducing a dual-mode cell death mechanism by apoptosis and ferropto-sis.Taken together,the in vitro and in vivo results demonstrate a successful strategy for improving the efficacy of PDT against hypoxic tumors.
基金supported by STI2030-Major Projects(2023ZD04069,Z231100003723004)the National Science Fund for Distinguished Young Scholars(32425041)the Chinese Universities Scientific Fund(2024TC165).
文摘Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.
基金This work was supported by grants from the National Natural Science Foundation of China(81570768 to Y.-C.W.,31900841 to Yan Li,91957205 and 31525012 to H.Y.,and 81471016 to J.J.)the Ministry of Science and Technology of China(2016YFA0500102 and 2016YFC1304905)CAS Key Laboratory of Nutrition,Metabolism and Food Safety(KLNMFS2019-01),and Chinese Academy of Sciences Interdisciplinary Innovation Team.
文摘Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.
基金supported by grants from National Key Research and Development Program of China(2021YFD1400704)the Sanya Institute of Nanjing Agricultural University(No.NAUSY-MS09).
文摘Broflanilide is a novel meta-diamide insecticide that acts as a y-aminobutyric acid-gated chloride channel allosteric modulator.With its unique mode of action,broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance.Establishing the base-line susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies.Here we evaluated the baseline susceptibil-ity to broflanilide for 3 cosmopolitan lepidopteran pest species,Helicoverpa armigera,Plutella xylostella,and Spodoptera frugiperda.Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China,with median lethal concentrations(LCso)ranging between 0.209 and 0.684,0.076 and 0.336,and 0.075 and 0.219 mg/L for H.armigera,P xylostella,and S.frugiperda,respectively.Among-population variability in susceptibility to broflanilide was moderate for H.armigera(3.3-fold),P xylostella(4.4-fold),and S.frugiperda(2.9-fold).The recommended diagnostic concentrations for H.armigera,P xylostella,and S.frugiperda were 8,4,and 2 mg/L,re-spectively.Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P xylostella and 1 spinosyns-resistant strain of S.frugiperda.Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.
基金funded by National Natural Science Foundation of China(No.52073045)the Key Scientific and Technological Project of Jilin Province(No.20190701010GH)+2 种基金the Development and Reform Commission of Jilin Province(No.2020C0355)the support from the Key Laboratory of Nanobiosensing and Nanobioanalysis at the Universities of Jilin Provincethe support from the Jilin Provincial Department of Education。
文摘We develop the effective modification strategy based on molecular engineering of s-triazine and its derivatives to improve the photoelectric performance of all-inorganic perovskites(AIP)for the first time.The surface modification strategy with cyanuric acid successfully increases the PLQY of AIP from 40.55%to 88.15%,and significantly enhances the current of the AIP film under 3 V by almost 20-fold(from4.44 m A to 81.20 m A).This work has proven the effectiveness of improving the photoelectric performances of AIP via s-triazine and its derivatives and also suggested the potential risks of reducing the photoelectric performance of AIP due to inappropriate substituents in conjugated organic ligands.
基金supported by the Natural Science Foundation of Shandong Province(ZR2019YQ11).
文摘A novel mechanism of the arylation of arenes via norbornene(NBE)relay palladation through meta-to para-selectivity was revealed via density functional theory(DFT)calculations.Our calculated results revealed that the reaction was initiated by a[mono-N-protected amino acid ligand(MPAA)–Pd]complex to activate at first the meta-C–H guided by the directing group(DG),and para-arylation was subsequently achieved by NBE relay palladation from meta-to para-position.Significantly,the palladium/norbornene(Pd/NBE)cooperative catalysis was catalyzed by a Pd–Ag bimetallic complex,which accounted for the experimental fact that no yield detected without Ag.The reaction pathway through para-to meta-selectivity was also investigated,while this pathway was kinetically unfavorable.The results revealed that the initial DG guided C–H site activation was the rate-determining step and played an important role in determining site-selectivity.The primary meta-activation was favorable in energy due to the less ring strain in the cyclic nitrile-coordinated C–H transition states in the meta position.Moreover,the perfect cooperation of a remote directing template and a transient mediator NBE through the alternating association with the Pd center achieved the relay through meta-to para-position.The present results provide a reasonable insight into the para-C–H arylation by the Pd/MPAA/NBE cooperative catalysis in conjunction with a precise DG and Ag(I)additive.
