With the rapid development of photo-responsive nanomaterials,photo-triggered therapeutic strategies such as photothermal therapy(PTT)and photodynamic therapy(PDT)have been new alternatives to current cancer therapeuti...With the rapid development of photo-responsive nanomaterials,photo-triggered therapeutic strategies such as photothermal therapy(PTT)and photodynamic therapy(PDT)have been new alternatives to current cancer therapeutic methods.Herein,we have fabricated oxygen vacancy-engineered BaTiO_(3)(BTO-Ov)nanoparticles(NPs)for near-infrared(NIR)light-triggered PTT,PDT,and catalytic therapy cooperatively for significantly improving cancer therapy.Compared to pristine BaTiO_(3)nanoparticles,BTO-Ov has stronger NIR light absorption and narrower band gap structure,which results in superior photothermal conversion and superoxide radical generation capabilities through PTT and PDT.Meanwhile,due to the existence of Ti^(3+),BTO-Ov also exhibits peroxidase(POD)-like activity to produce hydroxyl radical under tumor environment,which can be further improved under 808 nm light irradiation.Both in vitro and in vivo results demonstrate that such a multifunctional therapeutic nanoplatform can achieve a high therapeutic efficacy triggered by a single NIR light irradiation.The defect engineering strategy can be used as a general approach to fabricate multifunctional cancer therapeutic nanoplatform.展开更多
Arabinogalactan proteins(AGPs)are widely distributed in plant cells.Fasciclin-like AGPs(FLAs)belong to a subclass of AGPs that play important roles in plant growth and development.However,little is known about the bio...Arabinogalactan proteins(AGPs)are widely distributed in plant cells.Fasciclin-like AGPs(FLAs)belong to a subclass of AGPs that play important roles in plant growth and development.However,little is known about the biological functions of rice FLA.Herein,we report the identification of a male-sterile mutant of DEFECTIVE EXINE AND APERTURE PATTERNING1(DEAP1)in rice.The deap1 mutant anthers produced aberrant pollen grains with defective exine formation and a flattened aperture annulus and exhibited slightly delayed tapetum degradation.DEAP1 encodes a plasma membrane-associated member of groupⅢplant FLAs and is specifically and temporally expressed in reproductive cells and the tapetum layer during male development.Gene expression studies revealed reduced transcript accumulation of genes related to exine formation,aperture patterning,and tapetum development in deap1 mutants.Moreover,DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3 s(OsD6PKL3s),homologs of a known Arabidopsis aperture protein,to affect rice pollen aperture development.Our findings suggested that DEAP1 is involved in male reproductive development and may affect exine formation and aperture patterning,thereby providing new insights into the molecular functions of plant FLAs in male fertility.展开更多
基金the National Nature Science Foundation of China(Nos.82072065 and 81471784)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16021103)the National Youth Talent Support Program.
文摘With the rapid development of photo-responsive nanomaterials,photo-triggered therapeutic strategies such as photothermal therapy(PTT)and photodynamic therapy(PDT)have been new alternatives to current cancer therapeutic methods.Herein,we have fabricated oxygen vacancy-engineered BaTiO_(3)(BTO-Ov)nanoparticles(NPs)for near-infrared(NIR)light-triggered PTT,PDT,and catalytic therapy cooperatively for significantly improving cancer therapy.Compared to pristine BaTiO_(3)nanoparticles,BTO-Ov has stronger NIR light absorption and narrower band gap structure,which results in superior photothermal conversion and superoxide radical generation capabilities through PTT and PDT.Meanwhile,due to the existence of Ti^(3+),BTO-Ov also exhibits peroxidase(POD)-like activity to produce hydroxyl radical under tumor environment,which can be further improved under 808 nm light irradiation.Both in vitro and in vivo results demonstrate that such a multifunctional therapeutic nanoplatform can achieve a high therapeutic efficacy triggered by a single NIR light irradiation.The defect engineering strategy can be used as a general approach to fabricate multifunctional cancer therapeutic nanoplatform.
基金supported by funds from the National Natural Science Foundation of China(31901482,31971863,31971867 and 32171966)the Open Research Fund of the State Key Laboratory of Hybrid Rice(Hunan Hybrid Rice Research Centre,2016KF10)the Sichuan Province Science and Technology Support Program(2021YFYZ0016,2021YFYZ0027,2020YJ0139)。
文摘Arabinogalactan proteins(AGPs)are widely distributed in plant cells.Fasciclin-like AGPs(FLAs)belong to a subclass of AGPs that play important roles in plant growth and development.However,little is known about the biological functions of rice FLA.Herein,we report the identification of a male-sterile mutant of DEFECTIVE EXINE AND APERTURE PATTERNING1(DEAP1)in rice.The deap1 mutant anthers produced aberrant pollen grains with defective exine formation and a flattened aperture annulus and exhibited slightly delayed tapetum degradation.DEAP1 encodes a plasma membrane-associated member of groupⅢplant FLAs and is specifically and temporally expressed in reproductive cells and the tapetum layer during male development.Gene expression studies revealed reduced transcript accumulation of genes related to exine formation,aperture patterning,and tapetum development in deap1 mutants.Moreover,DEAP1 may interact with two rice D6 PROTEIN KINASE-LIKE3 s(OsD6PKL3s),homologs of a known Arabidopsis aperture protein,to affect rice pollen aperture development.Our findings suggested that DEAP1 is involved in male reproductive development and may affect exine formation and aperture patterning,thereby providing new insights into the molecular functions of plant FLAs in male fertility.
