African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild pigs.There is no effective vaccine,and the control of the disease relies mainly on surveillance and ...African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild pigs.There is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected pigs.Previously,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and p30.However,the antibodies against these proteins do not provide efficient protection against ASFV infection in pigs.Therefore,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still required.In this study,we expressed and purified a recombinant p B602 L protein.The purified p B602 L protein was then used as an antigen to develop an indirect ELISA assay.This assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine parvovirus.This assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV sera.The coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in pigs.The results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 days.Our results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.展开更多
[目的]用Meta分析的方法评价IL-8基因251 A/T位点的多态性与亚洲人群胃癌易感性的关系。[方法]检索Web of science、PubMed、EMBASE、万方数据库、中国生物医学文献数据库、中文科技期刊数据库、中国期刊全文数据库,日期均从各数据库开...[目的]用Meta分析的方法评价IL-8基因251 A/T位点的多态性与亚洲人群胃癌易感性的关系。[方法]检索Web of science、PubMed、EMBASE、万方数据库、中国生物医学文献数据库、中文科技期刊数据库、中国期刊全文数据库,日期均从各数据库开始建库至2018年1月。全面检索IL-8基因251 A/T位点的多态性与胃癌易感性的病例对照研究文献,采用STATA统计软件进行Meta分析。[结果]最终纳入15篇病例对照研究进行Meta分析,共计3738例胃癌患者、4497例健康对照者。分析结果显示,IL-8基因251 A/T位点在等位基因模型(A vs T:OR=1.12;95%CI为1.04~1.21;P=0.002)、共显性模型(AA vs AT:OR=1.15;95%CI为1.00~1.32;P=0.050)、相加模型(AA vs TT:OR=1.36;95%CI为1.18~1.57;P=0.000)、相加模型(AT vs TT:OR=1.23;95%CI为1.11~1.36;P=0.000)、显性模型(AA vs AT+TT:OR=1.23;95%CI为1.08~1.40;P=0.002)、隐性模型(TT vs AA+AT:OR=1.26;95%CI为1.15~1.39)下均与亚洲人群胃癌易感性有关。[结论]IL-8基因251 A/T位点多态性与亚洲人群胃癌的易感性相关。展开更多
基金supported by grants from the National Key R&D Program of China (2016YFD0500705, 2017YFD0500105, and 2017YFC1200502)the Fundamental Research Funds for the Central Publicinterest Research Institutes (Y2017LM08)。
文摘African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild pigs.There is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected pigs.Previously,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and p30.However,the antibodies against these proteins do not provide efficient protection against ASFV infection in pigs.Therefore,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still required.In this study,we expressed and purified a recombinant p B602 L protein.The purified p B602 L protein was then used as an antigen to develop an indirect ELISA assay.This assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine parvovirus.This assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV sera.The coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in pigs.The results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 days.Our results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.
文摘[目的]用Meta分析的方法评价IL-8基因251 A/T位点的多态性与亚洲人群胃癌易感性的关系。[方法]检索Web of science、PubMed、EMBASE、万方数据库、中国生物医学文献数据库、中文科技期刊数据库、中国期刊全文数据库,日期均从各数据库开始建库至2018年1月。全面检索IL-8基因251 A/T位点的多态性与胃癌易感性的病例对照研究文献,采用STATA统计软件进行Meta分析。[结果]最终纳入15篇病例对照研究进行Meta分析,共计3738例胃癌患者、4497例健康对照者。分析结果显示,IL-8基因251 A/T位点在等位基因模型(A vs T:OR=1.12;95%CI为1.04~1.21;P=0.002)、共显性模型(AA vs AT:OR=1.15;95%CI为1.00~1.32;P=0.050)、相加模型(AA vs TT:OR=1.36;95%CI为1.18~1.57;P=0.000)、相加模型(AT vs TT:OR=1.23;95%CI为1.11~1.36;P=0.000)、显性模型(AA vs AT+TT:OR=1.23;95%CI为1.08~1.40;P=0.002)、隐性模型(TT vs AA+AT:OR=1.26;95%CI为1.15~1.39)下均与亚洲人群胃癌易感性有关。[结论]IL-8基因251 A/T位点多态性与亚洲人群胃癌的易感性相关。
