Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(H...Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.展开更多
现有的波达方向(Direction of Arrival,DOA)估计方法大多依赖于阵列导向矩阵的精确无偏条件,而实际工程中由于时钟偏移、阵元位置偏差的存在导致该条件往往难以满足.为匹配阵列实际接收条件,本文基于部分校准嵌套阵列,提出了一种增益相...现有的波达方向(Direction of Arrival,DOA)估计方法大多依赖于阵列导向矩阵的精确无偏条件,而实际工程中由于时钟偏移、阵元位置偏差的存在导致该条件往往难以满足.为匹配阵列实际接收条件,本文基于部分校准嵌套阵列,提出了一种增益相位误差下的DOA估计新方法.该方法首先利用连乘子函数和简单的代数运算完成初始增益相位误差估计,然后通过协方差矩阵向量化和稀疏表示理论构建具有连续自由度的稀疏表示向量模型,最后考虑有效样本的影响,在初始增益相位误差估计的基础上应用稀疏总体最小均方(Sparse total least squares,STLS)算法完成波达方向估计.本文所提方法不仅对阵列增益相位误差不敏感,而且可依靠嵌套阵列高自由度特性和STLS算法的抗扰动特性获得改进的分辨率和估计精度,计算机仿真结果验证了所提算法的有效性.展开更多
文摘Objective INF2 is a member of the formins family.Abnormal expression and regulation of INF2 have been associated with the progression of various tumors,but the expression and role of INF2 in hepatocellular carcinoma(HCC)remain unclear.HCC is a highly lethal malignant tumor.Given the limitations of traditional treatments,this study explored the expression level,clinical value and potential mechanism of INF2 in HCC in order to seek new therapeutic targets.Methods In this study,we used public databases to analyze the expression of INF2 in pan-cancer and HCC,as well as the impact of INF2 expression levels on HCC prognosis.Quantitative real time polymerase chain reaction(RT-qPCR),Western blot,and immunohistochemistry were used to detect the expression level of INF2 in liver cancer cells and human HCC tissues.The correlation between INF2 expression and clinical pathological features was analyzed using public databases and clinical data of human HCC samples.Subsequently,the effects of INF2 expression on the biological function and Drp1 phosphorylation of liver cancer cells were elucidated through in vitro and in vivo experiments.Finally,the predictive value and potential mechanism of INF2 in HCC were further analyzed through database and immunohistochemical experiments.Results INF2 is aberrantly high expression in HCC samples and the high expression of INF2 is correlated with overall survival,liver cirrhosis and pathological differentiation of HCC patients.The expression level of INF2 has certain diagnostic value in predicting the prognosis and pathological differentiation of HCC.In vivo and in vitro HCC models,upregulated expression of INF2 triggers the proliferation and migration of the HCC cell,while knockdown of INF2 could counteract this effect.INF2 in liver cancer cells may affect mitochondrial division by inducing Drp1 phosphorylation and mediate immune escape by up-regulating PD-L1 expression,thus promoting tumor progression.Conclusion INF2 is highly expressed in HCC and is associated with poor prognosis.High expression of INF2 may promote HCC progression by inducing Drp1 phosphorylation and up-regulation of PD-L1 expression,and targeting INF2 may be beneficial for HCC patients with high expression of INF2.
文摘现有的波达方向(Direction of Arrival,DOA)估计方法大多依赖于阵列导向矩阵的精确无偏条件,而实际工程中由于时钟偏移、阵元位置偏差的存在导致该条件往往难以满足.为匹配阵列实际接收条件,本文基于部分校准嵌套阵列,提出了一种增益相位误差下的DOA估计新方法.该方法首先利用连乘子函数和简单的代数运算完成初始增益相位误差估计,然后通过协方差矩阵向量化和稀疏表示理论构建具有连续自由度的稀疏表示向量模型,最后考虑有效样本的影响,在初始增益相位误差估计的基础上应用稀疏总体最小均方(Sparse total least squares,STLS)算法完成波达方向估计.本文所提方法不仅对阵列增益相位误差不敏感,而且可依靠嵌套阵列高自由度特性和STLS算法的抗扰动特性获得改进的分辨率和估计精度,计算机仿真结果验证了所提算法的有效性.
