建立接骨木多指标成分定量控制方法,采用化学计量学、加权逼近理想排序(technique for order preference by similarity to ideal solution,TOPSIS)法与灰色关联度(grey relational analysis,GRA)融合模型评价不同产地接骨木质量差异,...建立接骨木多指标成分定量控制方法,采用化学计量学、加权逼近理想排序(technique for order preference by similarity to ideal solution,TOPSIS)法与灰色关联度(grey relational analysis,GRA)融合模型评价不同产地接骨木质量差异,提高接骨木药材的整体质量控制水平。采用Discovery C_(18)色谱柱,乙腈-0.1%磷酸为流动相梯度洗脱,采用变波长程序,建立槲皮素与没食子酸、原儿茶酸、芦丁、异槲皮苷、矢车菊素-3-O-葡萄糖苷、白桦脂酸、莫诺苷、齐墩果酸、熊果酸、蛇菰宁、落叶松树脂醇的相对校正因子,利用相对校正因子的均值测定各成分含量,对比外标法与一测多评法间差异;按《中华人民共和国药典》2020年版标准检测浸出物和总灰分。对18批接骨木各评价指标检测结果进行主成分分析(principal component analysis,PCA)和正交偏最小二乘方判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA),挖掘影响产品质量的差异性标志物。建立加权TOPSIS与GRA融合模型,对不同产地接骨木药材质量进行综合评价。12个成分线性关系(R^(2)>0.9992)、仪器精密度、重复性、稳定性(24 h)均良好。准确度高,平均加样回收率(n=9)在96.95%~100.1%之间,相对标准偏差<2.0%。相对校正因子稳健性良好,2种方法差异不大。PCA结果显示18批接骨木聚为3类,OPLS-DA结果显示蛇菰宁、齐墩果酸、白桦脂酸、异槲皮苷、没食子酸和矢车菊素-3-O-葡萄糖苷是影响接骨木产品质量的差异性标志物。18批样品的相对贴近度在0.2362~0.7172之间,表明接骨木存在地域差异性。所建方法便捷经济,准确实用,可实现接骨木多指标成分快速分析;化学计量学联合加权TOPSIS与GRA融合模型可用于不同产地接骨木的整体质量评价。展开更多
AIM: To investigate proteomic changes in spinal cord and dorsal root ganglia (DRG) of rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis. METHODS: The colonic myeloperoxidase (MPO) activity and tumor necro...AIM: To investigate proteomic changes in spinal cord and dorsal root ganglia (DRG) of rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis. METHODS: The colonic myeloperoxidase (MPO) activity and tumor necrosis factor-(TNF- ) level were determined. A two-dimensional electrophoresis (2-DE)-based proteomic technique was used to profile the global protein expression changes in the DRG and spinal cord of the rats with acute colitis induced by intracolonic injection of TNBS. RESULTS: TNBS group showed significantly elevated colonic MPO activity and increased TNF-level. The proteins derived from lumbosacral enlargement of the spinal cord and DRG were resolved by 2-DE; and 26 and 19 proteins that displayed significantly different expression levels in the DRG and spinal cord were identified respectively. Altered proteins were found to be involved in a number of biological functions, such as inflammation/immunity, cell signaling, redox regulation, sulfate transport and cellular metabolism. The over-expression of the protein similar to potassium channel tetramerisation domain containing protein 12 (Kctd 12) and low expression of proteasome subunit type-1 (psma) were validated by Western blotting analysis. CONCLUSION: TNBS-induced colitis has a profound impact on protein profiling in the nervous system. This result helps understand the neurological pathogenesis of inflammatory bowel disease.展开更多
The treatment of sorbic acid manufacturing wastewater by NDA-150 resin was investigated. The experiments show that this process is suitable for the treatment of sorbic acid manufacturing wastewater. About 98% sorbic a...The treatment of sorbic acid manufacturing wastewater by NDA-150 resin was investigated. The experiments show that this process is suitable for the treatment of sorbic acid manufacturing wastewater. About 98% sorbic acid and 50% CODcr were removed under optimal operating conditions, and 70% of sorbic acid in sorbic acid manufacturing wastewater was reclaimed. This process makes it possible to recover valuable materials from the wastewater and to improve the quality of effluent water.展开更多
文摘建立接骨木多指标成分定量控制方法,采用化学计量学、加权逼近理想排序(technique for order preference by similarity to ideal solution,TOPSIS)法与灰色关联度(grey relational analysis,GRA)融合模型评价不同产地接骨木质量差异,提高接骨木药材的整体质量控制水平。采用Discovery C_(18)色谱柱,乙腈-0.1%磷酸为流动相梯度洗脱,采用变波长程序,建立槲皮素与没食子酸、原儿茶酸、芦丁、异槲皮苷、矢车菊素-3-O-葡萄糖苷、白桦脂酸、莫诺苷、齐墩果酸、熊果酸、蛇菰宁、落叶松树脂醇的相对校正因子,利用相对校正因子的均值测定各成分含量,对比外标法与一测多评法间差异;按《中华人民共和国药典》2020年版标准检测浸出物和总灰分。对18批接骨木各评价指标检测结果进行主成分分析(principal component analysis,PCA)和正交偏最小二乘方判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA),挖掘影响产品质量的差异性标志物。建立加权TOPSIS与GRA融合模型,对不同产地接骨木药材质量进行综合评价。12个成分线性关系(R^(2)>0.9992)、仪器精密度、重复性、稳定性(24 h)均良好。准确度高,平均加样回收率(n=9)在96.95%~100.1%之间,相对标准偏差<2.0%。相对校正因子稳健性良好,2种方法差异不大。PCA结果显示18批接骨木聚为3类,OPLS-DA结果显示蛇菰宁、齐墩果酸、白桦脂酸、异槲皮苷、没食子酸和矢车菊素-3-O-葡萄糖苷是影响接骨木产品质量的差异性标志物。18批样品的相对贴近度在0.2362~0.7172之间,表明接骨木存在地域差异性。所建方法便捷经济,准确实用,可实现接骨木多指标成分快速分析;化学计量学联合加权TOPSIS与GRA融合模型可用于不同产地接骨木的整体质量评价。
基金Supported by The Research Grants Council of Hong Kong,RGC-HKBU2/07CThe Hong Kong Jockey Club Institute of Chinese Medicine, JCICM4-07
文摘AIM: To investigate proteomic changes in spinal cord and dorsal root ganglia (DRG) of rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis. METHODS: The colonic myeloperoxidase (MPO) activity and tumor necrosis factor-(TNF- ) level were determined. A two-dimensional electrophoresis (2-DE)-based proteomic technique was used to profile the global protein expression changes in the DRG and spinal cord of the rats with acute colitis induced by intracolonic injection of TNBS. RESULTS: TNBS group showed significantly elevated colonic MPO activity and increased TNF-level. The proteins derived from lumbosacral enlargement of the spinal cord and DRG were resolved by 2-DE; and 26 and 19 proteins that displayed significantly different expression levels in the DRG and spinal cord were identified respectively. Altered proteins were found to be involved in a number of biological functions, such as inflammation/immunity, cell signaling, redox regulation, sulfate transport and cellular metabolism. The over-expression of the protein similar to potassium channel tetramerisation domain containing protein 12 (Kctd 12) and low expression of proteasome subunit type-1 (psma) were validated by Western blotting analysis. CONCLUSION: TNBS-induced colitis has a profound impact on protein profiling in the nervous system. This result helps understand the neurological pathogenesis of inflammatory bowel disease.
文摘The treatment of sorbic acid manufacturing wastewater by NDA-150 resin was investigated. The experiments show that this process is suitable for the treatment of sorbic acid manufacturing wastewater. About 98% sorbic acid and 50% CODcr were removed under optimal operating conditions, and 70% of sorbic acid in sorbic acid manufacturing wastewater was reclaimed. This process makes it possible to recover valuable materials from the wastewater and to improve the quality of effluent water.
